Status
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Gene information
General information
TC033552
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009505
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 179622..182600
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033552
    iBeetle screen iB_08621

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033552 RA
      MTSEEKIAD YEVFITTMH INLGWSPLH CMIEKKKSS 
      LAQTLINQN VDINSLDKF RRSPLSIAS AKNNNSLVE 
      ILLSSGAKV NRGFPLHYA CEKNHVKIA QTLIENGAD 
      LNLERDSNY PLLIAINKG HIETAIFLL QNGADVHIV 
      DKHKETALH WACDRGLEL LIQPLINNG CDIDAKDRS 
      EMTPLNICL VRQENRPDL VKLLIKNGA LISLRDSTG 
      CTYLHSCFN QDSLTEFLK HGLDPNLQD VNNETPLYS 
      HSRFTKHDY CNILLEYGA DPNIARKGG WTPLHVAAS 
      EGYLDYVKE LIKHKASVN AASAQNITP LHLAAKFGH 
      LEIVKELIK VGAFIDIPI IDLCDVNSS LPHFVDSEW 
      VIEGQSPFD LATKFENFE ICDFLIATR KNTSGSSLM 
      GWTLLHHSV WSGFVDLPN SAVVNVCDK FGRTPLSLA 
      AAKNNSDIV KKLHKAGGD VDLGLPLHF AVRSEALDC 
      VSLLLDLKC DVNKTSNHE FENTPLLDA IYESKINSA 
      MALIRHKID VNCTNKWGE TPLELACRY TNLDLAKEL 
      LNCGACVNS TNFCKETPL NVAALFKNI DILSLLIGS 
      NSDVNFRDH FDCTPLHSA VGENFEAGV LKLVKAGAA 
      VNVCNDDFE TPLILAVDN ANLNVTKIL IDHNSDVNF 
      QTPEGWSAL HTSVYCGNL NCVKELLKS GAEVNVASS 
      ENVLPIYLA AIKGWDSVV EELLDRGSL LDPYIFEYG 
      VFRDDTGSF SKFETYHIV AGNTPLLGG CAKGHLGVV 
      KLLESRGAS LNCVNSWGN NVLHEAVLS QSVEVVEFL 
      LGLNADFNV GNKSGKTPL DLAKEINTE TIINLIINK 
      PI
    CDS sequence
    • >TC033552 RA
      atgacttca gaagaaaaa atcgcagat tatgaagtg 
      ttcatcacc acgatgcac ataaattta ggatggtcc 
      cctttacat tgcatgatt gagaaaaaa aaaagttcg 
      cttgcccaa actctaata aatcaaaat gtggacata 
      aactccttg gacaaattc cgtcgctct cctctatct 
      atcgccagc gctaagaac aataactcc ctagttgaa 
      attttacta tcatctggt gcaaaagtc aatcgcgga 
      tttcctctt cactacgcg tgtgaaaaa aaccacgta 
      aaaattgcg caaactctt attgaaaac ggcgccgat 
      ttaaattta gaacgcgac agtaattac ccacttctg 
      atagcgata aacaaggga cacatcgaa acggcaatt 
      tttttatta caaaacgga gctgatgtt catattgtt 
      gataaacac aaagaaacg gcccttcat tgggcttgc 
      gaccggggc ttggaactt ttaatccaa cccttgatc 
      aacaatgga tgtgatata gatgccaaa gatcgctct 
      gaaatgacc cctttaaac atatgttta gttcgtcaa 
      gaaaaccga ccagatttg gtcaaactc ttgatcaaa 
      aacggagct ttaattagt ttaagggac agtacaggt 
      tgcacttac ttgcactct tgtttcaat caagattca 
      ctgaccgag tttttaaaa catggactt gacccgaat 
      ttacaagat gtgaataac gaaacacca ctttattca 
      cattcaagg tttacaaaa catgattat tgtaatatt 
      ttgcttgag tatggtgct gatccaaac attgcgcga 
      aaagggggc tggactcca ctacatgta gcagcaagt 
      gagggttat ttagactac gtcaaagaa ttaatcaaa 
      cacaaagcg agtgttaac gcagctagt gctcaaaat 
      atcactccc ttacaccta gctgcaaaa tttggacat 
      cttgaaatt gtgaaggag ttaatcaaa gtgggggct 
      tttattgac attccgatc atagatttg tgtgatgtt 
      aactcgtcc ctgccgcat tttgtggac tctgagtgg 
      gtgattgag ggtcaatct ccgtttgat ttggctaca 
      aaattcgaa aacttcgaa atttgtgat tttcttatt 
      gcaaccaga aaaaatacg tctgggagt agtttgatg 
      gggtggact cttttgcac cactctgtg tggagtgga 
      ttcgtcgat ttgcccaat tctgctgtt gttaacgtt 
      tgtgataaa tttggccga actccttta tcgttagct 
      gcagctaag aataattca gatatagtc aaaaaattg 
      cacaaggca ggtggtgat gtggattta ggactgcct 
      ttacatttt gcggtccgt agcgaggcc ttagattgt 
      gtttcccta cttttggat ttgaaatgt gatgttaac 
      aagacttct aatcacgaa tttgaaaat acaccgctc 
      ctggacgcg atttatgaa tcgaaaatt aactcagcg 
      atggcttta attcgtcac aagattgac gtaaattgt 
      actaacaaa tggggcgaa acgccactt gaacttgct 
      tgcagatac accaattta gatttggct aaagaactg 
      ttaaactgt ggagcctgc gttaactcg accaatttt 
      tgtaaagaa acgcctcta aacgtggca gctctgttc 
      aaaaacatc gacattttg agtttactt attggcagt 
      aacagtgat gttaatttt cgagatcat tttgattgt 
      acaccttta catagtgca gttggagaa aatttcgaa 
      gctggagtt ttgaaactt gtaaaagcg ggggctgcg 
      gtaaatgtt tgcaatgac gattttgaa actccccta 
      atcctagct gttgataac gcgaattta aacgtgact 
      aaaattttg attgaccac aacagtgat gttaatttt 
      caaacaccg gaaggttgg agtgccctt catacaagt 
      gtctactgt ggaaattta aactgcgtg aaagagttg 
      ttaaaaagt ggggccgaa gttaatgtt gcaagtagt 
      gaaaatgtg cttccgatt tatttagct gctattaag 
      gggtgggac agtgtggtt gaagagttg ttggatagg 
      gggagtttg ttggatcct tatattttc gaatatggt 
      gtttttaga gacgacact gggagtttt tccaagttt 
      gaaacgtat cacattgtg gccgggaat acgcctctg 
      cttgggggt tgtgctaag gggcacttg ggtgtagtt 
      aaattgtta gagtcacgt ggggcgtct ttaaactgt 
      gttaatagt tgggggaat aatgtattg catgaagcg 
      gttttgagc caaagtgtg gaagttgtg gagttttta 
      ttgggatta aatgccgat tttaacgta gggaataaa 
      agtgggaag acgcctcta gatttggcc aaagaaata 
      aatactgaa actattatt aatttaatt attaataaa 
      ccaatttaa
    mRNA sequence
    • >TC033552 RA
      accgataaa taaattaat aacagtgta gtgagtcat 
      tcgataatt tttatagtc tgaataaaa tcgttgaca 
      tcgctaaat agtgaggta cctacctac gacgaatta 
      ttatttgtg ttattatat gtgtaaaag acgcgagaa 
      attaaatct gttttacaa aaaaaaaaa ttacgtatg 
      aatattacg ccataagtt gcaatgact tcagaagaa 
      aaaatcgca gattatgaa gtgttcatc accacgatg 
      cacataaat ttaggatgg tccccttta cattgcatg 
      attgagaaa aaaaaaagt tcgcttgcc caaactcta 
      ataaatcaa aatgtggac ataaactcc ttggacaaa 
      ttccgtcgc tctcctcta tctatcgcc agcgctaag 
      aacaataac tccctagtt gaaatttta ctatcatct 
      ggtgcaaaa gtcaatcgc ggatttcct cttcactac 
      gcgtgtgaa aaaaaccac gtaaaaatt gcgcaaact 
      cttattgaa aacggcgcc gatttaaat ttagaacgc 
      gacagtaat tacccactt ctgatagcg ataaacaag 
      ggacacatc gaaacggca attttttta ttacaaaac 
      ggagctgat gttcatatt gttgataaa cacaaagaa 
      acggccctt cattgggct tgcgaccgg ggcttggaa 
      cttttaatc caacccttg atcaacaat ggatgtgat 
      atagatgcc aaagatcgc tctgaaatg accccttta 
      aacatatgt ttagttcgt caagaaaac cgaccagat 
      ttggtcaaa ctcttgatc aaaaacgga gctttaatt 
      agtttaagg gacagtaca ggttgcact tacttgcac 
      tcttgtttc aatcaagat tcactgacc gagttttta 
      aaacatgga cttgacccg aatttacaa gatgtgaat 
      aacgaaaca ccactttat tcacattca aggtttaca 
      aaacatgat tattgtaat attttgctt gagtatggt 
      gctgatcca aacattgcg cgaaaaggg ggctggact 
      ccactacat gtagcagca agtgagggt tatttagac 
      tacgtcaaa gaattaatc aaacacaaa gcgagtgtt 
      aacgcagct agtgctcaa aatatcact cccttacac 
      ctagctgca aaatttgga catcttgaa attgtgaag 
      gagttaatc aaagtgggg gcttttatt gacattccg 
      atcatagat ttgtgtgat gttaactcg tccctgccg 
      cattttgtg gactctgag tgggtgatt gagggtcaa 
      tctccgttt gatttggct acaaaattc gaaaacttc 
      gaaatttgt gattttctt attgcaacc agaaaaaat 
      acgtctggg agtagtttg atggggtgg actcttttg 
      caccactct gtgtggagt ggattcgtc gatttgccc 
      aattctgct gttgttaac gtttgtgat aaatttggc 
      cgaactcct ttatcgtta gctgcagct aagaataat 
      tcagatata gtcaaaaaa ttgcacaag gcaggtggt 
      gatgtggat ttaggactg cctttacat tttgcggtc 
      cgtagcgag gccttagat tgtgtttcc ctacttttg 
      gatttgaaa tgtgatgtt aacaagact tctaatcac 
      gaatttgaa aatacaccg ctcctggac gcgatttat 
      gaatcgaaa attaactca gcgatggct ttaattcgt 
      cacaagatt gacgtaaat tgtactaac aaatggggc 
      gaaacgcca cttgaactt gcttgcaga tacaccaat 
      ttagatttg gctaaagaa ctgttaaac tgtggagcc 
      tgcgttaac tcgaccaat ttttgtaaa gaaacgcct 
      ctaaacgtg gcagctctg ttcaaaaac atcgacatt 
      ttgagttta cttattggc agtaacagt gatgttaat 
      tttcgagat cattttgat tgtacacct ttacatagt 
      gcagttgga gaaaatttc gaagctgga gttttgaaa 
      cttgtaaaa gcgggggct gcggtaaat gtttgcaat 
      gacgatttt gaaactccc ctaatccta gctgttgat 
      aacgcgaat ttaaacgtg actaaaatt ttgattgac 
      cacaacagt gatgttaat tttcaaaca ccggaaggt 
      tggagtgcc cttcataca agtgtctac tgtggaaat 
      ttaaactgc gtgaaagag ttgttaaaa agtggggcc 
      gaagttaat gttgcaagt agtgaaaat gtgcttccg 
      atttattta gctgctatt aaggggtgg gacagtgtg 
      gttgaagag ttgttggat agggggagt ttgttggat 
      ccttatatt ttcgaatat ggtgttttt agagacgac 
      actgggagt ttttccaag tttgaaacg tatcacatt 
      gtggccggg aatacgcct ctgcttggg ggttgtgct 
      aaggggcac ttgggtgta gttaaattg ttagagtca 
      cgtggggcg tctttaaac tgtgttaat agttggggg 
      aataatgta ttgcatgaa gcggttttg agccaaagt 
      gtggaagtt gtggagttt ttattggga ttaaatgcc 
      gattttaac gtagggaat aaaagtggg aagacgcct 
      ctagatttg gccaaagaa ataaatact gaaactatt 
      attaattta attattaat aaaccaatt taattgtta 
      ttgaatgaa cgaataaat attttttgt cgatttttt 
      tttaatttg ttggatcta taaagttac tgaaaatat 
      gtaaaacca caggtaaaa ctttaaatt taaaaatac 
      tacaatata agtgtaagt actaattcg aaacagcga 
      aaacagcca attattatt atggctttt atttatagt 
      tgtattttt gtttgctaa aataacatt aaaaaatga 
      aaatggta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY