Status
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Gene information
General information
TC009314 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 264956..270713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009314
    iBeetle screen iB_08619

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009314 RA
      MNLIEKFIV VFFSLQCVS AIKDNSKDF IFCAKAAGT 
      ITHISEDGG PEEISTESP DEKCVKSYC YTLWKEDPN 
      DGTKTIMGQ GCWDTSGKP NECDRAQCL AANRPPKAM 
      NNTKFCCCS EDMCNVNFT DAYVPVEDF PPSTTEVAP 
      KKSVKSFIW IVVVVSFIF VTVIAFGAS YYLWKKKPK 
      KHDMEGGQH AMPPPADYS LDKLKLLNV IGQGRYGCV 
      WRGMIDDQE VAVKVFAAH HRNYFLNEH EIYKLSGEN 
      SALLKCYGG GEYITAPGG LTDYRLLLS LEQECLQEY 
      LKNHTLDLM TLSKMSLGV ARGLAHLHS DLGKPCIAH 
      RDINSRNIL VRPDLTCCI CDLGLAVIP RLAENKSIS 
      EAGTLRYMA PEVLEGAVN LRDCESALK QIDVYALGL 
      VLWELGIRC SDMQNSEPS PYSPPFFKE AGENPTLEQ 
      MQTLVSRHK ARPLWPPSW KDTAAARLL CETAEDCWD 
      QDAEARLTS LCVVERLLE LPSLKGRVL HSMHPPASP 
      TPLINNNHL HDHQIDISV GTVETLLSP SEEHCKNSN 
      QLVACVTPL QPYQGRNPC LERNLLSGS SDSLLIDKS 
      SKHCTSSEF QNLITNDFL NYQINYRAT PIPYLQNAV 
      HGSPKQSNT SSESSKSRF KWNGFMKLF PNKKYNSGD 
      NSTKATQVK LNAKLVNGQ NGVTTSLLS SDSETKRPS 
      TLPLNVVKS QENSCGVTQ ILKRKNSLS RQRSLEQFN 
      EVFSSISDL SRLKDPSLR IKTPGDVPP SVRRTRGKA 
      AKESARFSL YDDRMMSRG QWGSVPDLE PPLPPKLPQ 
      LNDLQDRDS VSSF
    CDS sequence
    • >TC009314 RA
      atgaatctt attgagaag tttatagtt gttttcttt 
      tcgctgcaa tgtgtcagt gccattaaa gataattcc 
      aaagatttt atattttgt gcaaaagct gcagggact 
      attacacac attagtgaa gatgggggt cctgaagaa 
      atatcaacg gagtctcca gacgaaaag tgcgtcaag 
      agttactgc tacaccctg tggaaagag gatccaaac 
      gatggcacc aaaaccatc atggggcaa ggttgttgg 
      gacacttct ggcaaaccg aacgaatgt gacagggca 
      caatgtctt gccgccaat cggcctccc aaagcaatg 
      aacaacacg aaattttgt tgttgttct gaagacatg 
      tgcaatgtt aactttacc gatgcttac gtacctgtc 
      gaagacttc cctccatcc acgaccgaa gtggcgccg 
      aagaagtcg gtcaaatcg ttcatttgg atcgttgta 
      gttgtttcg ttcatattt gtaacggtt attgctttt 
      ggagcctcg tactatctc tggaagaag aaaccgaag 
      aaacatgac atggaggga ggtcagcat gcaatgccg 
      ccaccggct gactacagc ttggacaaa ttgaagctt 
      ttgaacgtt attggtcaa gggagatat ggctgcgta 
      tggcgcgga atgatcgat gatcaggaa gtggccgtc 
      aaagtattt gcagctcat catcgcaat tactttctc 
      aacgaacat gaaatttac aaactctca ggtgaaaat 
      tcagcctta ttgaaatgc tacggaggt ggcgaatat 
      attaccgcc cccggtggt ttaactgac taccgtctc 
      cttctcagt cttgagcag gaatgcttg caggaatac 
      ctcaaaaat cacactcta gacttgatg actttgagt 
      aaaatgagt ttgggggtg gcgagaggt ttagcacat 
      ttacattcg gatttggga aagccatgt attgcgcat 
      agggatatc aacagtcgg aatattcta gttagaccc 
      gatttgacg tgctgtatt tgcgatttg gggttggcg 
      gtgataccg agacttgcg gagaacaag tccatcagt 
      gaagctgga actctgcga tacatggcc ccggaagtt 
      ttggaagga gcagtcaat ttgcgagat tgcgaaagt 
      gctctcaag caaatcgac gtttacgcc cttggcctt 
      gtcctatgg gaattagga atacgatgc agtgatatg 
      cagaattct gaaccatcg ccatattca ccgccattt 
      ttcaaggaa gctggagaa aatccaact ttagaacag 
      atgcaaact ttagtcagt cgacacaaa gcaaggcct 
      ttgtggccg ccgtcgtgg aaagacacg gctgcagcg 
      aggctctta tgtgaaacc gccgaggac tgctgggac 
      caggatgct gaagcccgt ttaacatca ctgtgtgtc 
      gtcgaacgt ctcctagaa ctcccttcg ctcaaaggg 
      cgcgttctt cattccatg caccctcca gcaagtccc 
      accccctta ataaacaac aaccacctt cacgaccac 
      caaatcgac atttccgtc ggtaccgta gaaaccctc 
      ttgtcgcca agcgaagag cattgtaaa aactccaat 
      caattagta gcctgtgta acgccactt caaccctac 
      caaggacgc aatccttgc cttgagcgg aaccttctt 
      tccggctcg agtgactct cttcttatt gataaatcg 
      tcgaaacat tgcacaagt tccgaattt cagaattta 
      atcacaaat gattttctc aactaccag attaattac 
      cgagccact cctattccc tatctccaa aatgcagtc 
      cacggtagc cctaaacag tcaaacact tcaagtgaa 
      agctccaag tctcgattc aaatggaac ggttttatg 
      aaactcttc ccgaataaa aaatacaat tcgggtgat 
      aattcgacg aaagccact caagtaaaa ctcaatgcg 
      aaattagtt aatggccaa aacggcgtc acaacttca 
      ctactatca tccgacagc gaaactaaa aggccttca 
      acgttgcca ttgaacgta gtcaaatca caggaaaac 
      tcctgtgga gttacacaa attctaaaa cggaaaaat 
      agtttatcg cgtcagagg tctctcgaa cagttcaat 
      gaagtattc tcgtcgatt agcgatctt tccagactt 
      aaagatcca tcactgaga attaaaaca ccaggtgat 
      gtaccgccg tctgtcaga aggacaagg ggtaaagcg 
      gctaaagaa tcagcaaga ttttccttg tatgatgat 
      aggatgatg agtcgaggc cagtggggc agtgtccct 
      gatttggag ccccctctg cccccgaag ttgccccaa 
      ttaaacgat ttgcaagac agagatagt gtgagcagt 
      ttttag
    mRNA sequence
    • >TC009314 RA
      gtttccgca taaaagatt cagatttgt gtttacttt 
      attttgaaa aaaattgtt tcgtttttt ggtaataaa 
      aataaacca tttcaaggc gggtctgtt ccagcagac 
      cggcgccac actaatttt tcaggcggg tattttcaa 
      tcacgtgac gtaacggta ggcggtgac tacggccac 
      tatagtcga catgactga ttgttgtga taaaaagcg 
      aacattaaa gaatgttat ttaagcgat tccgtgaga 
      gcatagttc agaaaacgt ctggttctg ataacacaa 
      caaatccat attttcatg ttgtttcac ttgtgtcga 
      acgattaaa acactagtg tggttaagt tcggctgtg 
      cgaatcaag tgtgcctga attcaaaca aaacactac 
      acaattttg actatgaat aaaagggac ctgcagaaa 
      tgaatctta ttgagaagt ttatagttg ttttctttt 
      cgctgcaat gtgtcagtg ccattaaag ataattcca 
      aagatttta tattttgtg caaaagctg cagggacta 
      ttacacaca ttagtgaag atgggggtc ctgaagaaa 
      tatcaacgg agtctccag acgaaaagt gcgtcaaga 
      gttactgct acaccctgt ggaaagagg atccaaacg 
      atggcacca aaaccatca tggggcaag gttgttggg 
      acacttctg gcaaaccga acgaatgtg acagggcac 
      aatgtcttg ccgccaatc ggcctccca aagcaatga 
      acaacacga aattttgtt gttgttctg aagacatgt 
      gcaatgtta actttaccg atgcttacg tacctgtcg 
      aagacttcc ctccatcca cgaccgaag tggcgccga 
      agaagtcgg tcaaatcgt tcatttgga tcgttgtag 
      ttgtttcgt tcatatttg taacggtta ttgcttttg 
      gagcctcgt actatctct ggaagaaga aaccgaaga 
      aacatgaca tggagggag gtcagcatg caatgccgc 
      caccggctg actacagct tggacaaat tgaagcttt 
      tgaacgtta ttggtcaag ggagatatg gctgcgtat 
      ggcgcggaa tgatcgatg atcaggaag tggccgtca 
      aagtatttg cagctcatc atcgcaatt actttctca 
      acgaacatg aaatttaca aactctcag gtgaaaatt 
      cagccttat tgaaatgct acggaggtg gcgaatata 
      ttaccgccc ccggtggtt taactgact accgtctcc 
      ttctcagtc ttgagcagg aatgcttgc aggaatacc 
      tcaaaaatc acactctag acttgatga ctttgagta 
      aaatgagtt tgggggtgg cgagaggtt tagcacatt 
      tacattcgg atttgggaa agccatgta ttgcgcata 
      gggatatca acagtcgga atattctag ttagacccg 
      atttgacgt gctgtattt gcgatttgg ggttggcgg 
      tgataccga gacttgcgg agaacaagt ccatcagtg 
      aagctggaa ctctgcgat acatggccc cggaagttt 
      tggaaggag cagtcaatt tgcgagatt gcgaaagtg 
      ctctcaagc aaatcgacg tttacgccc ttggccttg 
      tcctatggg aattaggaa tacgatgca gtgatatgc 
      agaattctg aaccatcgc catattcac cgccatttt 
      tcaaggaag ctggagaaa atccaactt tagaacaga 
      tgcaaactt tagtcagtc gacacaaag caaggcctt 
      tgtggccgc cgtcgtgga aagacacgg ctgcagcga 
      ggctcttat gtgaaaccg ccgaggact gctgggacc 
      aggatgctg aagcccgtt taacatcac tgtgtgtcg 
      tcgaacgtc tcctagaac tcccttcgc tcaaagggc 
      gcgttcttc attccatgc accctccag caagtccca 
      cccccttaa taaacaaca accaccttc acgaccacc 
      aaatcgaca tttccgtcg gtaccgtag aaaccctct 
      tgtcgccaa gcgaagagc attgtaaaa actccaatc 
      aattagtag cctgtgtaa cgccacttc aaccctacc 
      aaggacgca atccttgcc ttgagcgga accttcttt 
      ccggctcga gtgactctc ttcttattg ataaatcgt 
      cgaaacatt gcacaagtt ccgaatttc agaatttaa 
      tcacaaatg attttctca actaccaga ttaattacc 
      gagccactc ctattccct atctccaaa atgcagtcc 
      acggtagcc ctaaacagt caaacactt caagtgaaa 
      gctccaagt ctcgattca aatggaacg gttttatga 
      aactcttcc cgaataaaa aatacaatt cgggtgata 
      attcgacga aagccactc aagtaaaac tcaatgcga 
      aattagtta atggccaaa acggcgtca caacttcac 
      tactatcat ccgacagcg aaactaaaa ggccttcaa 
      cgttgccat tgaacgtag tcaaatcac aggaaaact 
      cctgtggag ttacacaaa ttctaaaac ggaaaaata 
      gtttatcgc gtcagaggt ctctcgaac agttcaatg 
      aagtattct cgtcgatta gcgatcttt ccagactta 
      aagatccat cactgagaa ttaaaacac caggtgatg 
      taccgccgt ctgtcagaa ggacaaggg gtaaagcgg 
      ctaaagaat cagcaagat tttccttgt atgatgata 
      ggatgatga gtcgaggcc agtggggca gtgtccctg 
      atttggagc cccctctgc ccccgaagt tgccccaat 
      taaacgatt tgcaagaca gagatagtg tgagcagtt 
      tttaggtat gtgtgtgga ttagagtga gttgtattt 
      atgtagagt ggctgagat tttgtaaat tatttttta 
      tttaagtgc aggccaatt tgttcataa tccaaaaat 
      taagaaaaa tactgctat tttctgcat ggaccagac 
      ttacttcca cttggagaa ggtttaaac aattgaaaa 
      a
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