Status
Please wait ...
Gene information
General information
TC009490 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 363220..367664
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009490
    iBeetle screen iB_08616

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009490 RA
      MGFESDSPI HTVWKSDLY RMQLAAPVP IPIISDEVP 
      PDLPSFLGR DYHGAITHI QAQNLLSPH ENGAYLVRS 
      SRSANGQFH TLSLKFNDK IHHYKLFYD PNSGLYVRE 
      KRYDCVRSL VADGLVTMY LELKAPLIL QRLATANYQ 
      ESPYMTLNK RKLRALSKE HAKNKNYVK DSNIPPNIP 
      LSQQILGPN FPEKYEKQH AFKLTTFKG LNWCELCGN 
      FLWGFTFQG KKCDDCGMI AHVRCSEKF PNDCVPDLK 
      YLRGVFGIE LTTLLTAHN AKLPFVVTK CVAEVEARG 
      LTTEGIYRL SGFAEEIEA IKMAFDKDG DKADLSQEK 
      YPNVNVITG ALKSYLRLL PIPLITFIV HPLLIDAMQ 
      HKNHDLKMS SVKHALKSL PKAHYDTLK YMIEHLHRV 
      SLHSAINKM NSHNLATVF APTLIGPSE SASDVMPDM 
      TSDILLIET LITHCERIF KN
    • >TC009490 RB
      MVGVGIFNL NKLDMEACC NYLYPPSFD FLRLYRMQL 
      AAPVPIPII SDEVPPDLP SFLGRDYHG AITHIQAQN 
      LLSPHENGA YLVRSSRSA NGQFHTLSL KFNDKIHHY 
      KLFYDPNSG LYVREKRYD CVRSLVADG LVTMYLELK 
      APLILQRLA TANYQESPY MTLNKRKLR ALSKEHAKN 
      KNYVKDSNI PPNIPLSQQ ILGPNFPEK YEKQHAFKL 
      TTFKGLNWC ELCGNFLWG FTFQGKKCD DCGMIAHVR 
      CSEKFPNDC VPDLKYLRG VFGIELTTL LTAHNAKLP 
      FVVTKCVAE VEARGLTTE GIYRLSGFA EEIEAIKMA 
      FDKDGDKAD LSQEKYPNV NVITGALKS YLRLLPIPL 
      ITFIVHPLL IDAMQHKNH DLKMSSVKH ALKSLPKAH 
      YDTLKYMIE HLHRVSLHS AINKMNSHN LATVFAPTL 
      IGPSESASD VMPDMTSDI LLIETLITH CERIFKN
    CDS sequence
    • >TC009490 RA
      atgggtttc gagtcggat tccccgatc cacacagtt 
      tggaagagt gacctgtac cgtatgcag ctggctgct 
      cccgtccct attcccata atcagtgac gaggtgcct 
      cccgatttg cccagtttt ttgggccgc gactaccac 
      ggggctatc acgcacatc caggcccag aatctcctc 
      agcccccat gaaaatggg gcttatttg gtgcgcagc 
      agtcgctcg gccaacggc cagtttcac accttgagt 
      ctcaaattt aacgataaa attcatcat tacaaactt 
      ttctatgac ccaaattcg gggctttat gttagggaa 
      aaacgctat gactgtgtc aggtcgtta gtggctgat 
      ggcttagtc acaatgtat ctcgaactt aaggcacct 
      ttaattttg caaagactt gccaccgct aattatcaa 
      gagagtcct tacatgacg ttaaataag cgcaagttg 
      agggcttta agcaaagaa catgccaaa aataaaaat 
      tacgtgaaa gattcaaac attccgcca aatattcca 
      ttaagtcag cagatatta gggcccaat tttccagag 
      aaatatgaa aaacaacac gcgtttaaa ttgacaacg 
      tttaagggg cttaattgg tgtgaattg tgtgggaat 
      tttttgtgg ggctttacg tttcaagga aagaaatgt 
      gatgattgt ggaatgata gctcatgtt cgctgttct 
      gagaaattc cccaacgat tgtgtcccc gacttgaaa 
      tatcttcgt ggagtgttt ggtattgaa ctgaccaca 
      cttttaaca gcccacaat gccaaatta ccatttgtg 
      gtaacgaaa tgtgtggct gaagttgaa gctagagga 
      cttacaacg gagggaatt tatcgtttg agtggtttt 
      gctgaagaa attgaagcg attaaaatg gctttcgat 
      aaagatggc gataaagcc gatttaagt caggaaaaa 
      tacccaaat gttaatgta ataacaggg gcgttaaaa 
      tcatatctt agactttta ccaattccg cttattaca 
      tttatcgtt catccactt ctcattgac gccatgcaa 
      cataaaaat cacgatttg aaaatgtcg tcagttaag 
      cacgccctt aaatcattg ccgaaagca cattatgat 
      actttgaaa tacatgatt gagcattta cacagggtg 
      tctttacat tcggcaata aataaaatg aattctcac 
      aacttggca actgttttt gccccaact ctgattgga 
      ccaagcgaa tctgcaagc gacgtaatg cctgatatg 
      acaagtgat attttatta attgaaact ctcatcaca 
      cattgtgaa cgtattttt aaaaattag
    • >TC009490 RB
      atggtcggt gttggaatt ttcaattta aataaacta 
      gacatggag gcgtgttgc aactacttg taccctccg 
      tctttcgat ttcttgcgc ttgtaccgt atgcagctg 
      gctgctccc gtccctatt cccataatc agtgacgag 
      gtgcctccc gatttgccc agttttttg ggccgcgac 
      taccacggg gctatcacg cacatccag gcccagaat 
      ctcctcagc ccccatgaa aatggggct tatttggtg 
      cgcagcagt cgctcggcc aacggccag tttcacacc 
      ttgagtctc aaatttaac gataaaatt catcattac 
      aaacttttc tatgaccca aattcgggg ctttatgtt 
      agggaaaaa cgctatgac tgtgtcagg tcgttagtg 
      gctgatggc ttagtcaca atgtatctc gaacttaag 
      gcaccttta attttgcaa agacttgcc accgctaat 
      tatcaagag agtccttac atgacgtta aataagcgc 
      aagttgagg gctttaagc aaagaacat gccaaaaat 
      aaaaattac gtgaaagat tcaaacatt ccgccaaat 
      attccatta agtcagcag atattaggg cccaatttt 
      ccagagaaa tatgaaaaa caacacgcg tttaaattg 
      acaacgttt aaggggctt aattggtgt gaattgtgt 
      gggaatttt ttgtggggc tttacgttt caaggaaag 
      aaatgtgat gattgtgga atgatagct catgttcgc 
      tgttctgag aaattcccc aacgattgt gtccccgac 
      ttgaaatat cttcgtgga gtgtttggt attgaactg 
      accacactt ttaacagcc cacaatgcc aaattacca 
      tttgtggta acgaaatgt gtggctgaa gttgaagct 
      agaggactt acaacggag ggaatttat cgtttgagt 
      ggttttgct gaagaaatt gaagcgatt aaaatggct 
      ttcgataaa gatggcgat aaagccgat ttaagtcag 
      gaaaaatac ccaaatgtt aatgtaata acaggggcg 
      ttaaaatca tatcttaga cttttacca attccgctt 
      attacattt atcgttcat ccacttctc attgacgcc 
      atgcaacat aaaaatcac gatttgaaa atgtcgtca 
      gttaagcac gcccttaaa tcattgccg aaagcacat 
      tatgatact ttgaaatac atgattgag catttacac 
      agggtgtct ttacattcg gcaataaat aaaatgaat 
      tctcacaac ttggcaact gtttttgcc ccaactctg 
      attggacca agcgaatct gcaagcgac gtaatgcct 
      gatatgaca agtgatatt ttattaatt gaaactctc 
      atcacacat tgtgaacgt atttttaaa aattag
    mRNA sequence
    • >TC009490 RA
      tttgcttca tgataaaaa tgcaatata ttgccaatc 
      gataagtcc acacgtgtt tattattgc atacactat 
      atttattta tacataaag cacaaaaag cacgcctat 
      gataaaagg aagttggac gcaccttta aggagcaag 
      aaacgataa caagccaag ttactgcca aacattatt 
      ccaaatatt tctcaggaa tgtggtaag agtgtcagt 
      ggaataaaa ttaggccaa aggagataa gacccaaac 
      tgcgttcac ctatattat gttataagt gtataatgt 
      agttttagg cagaataga gcaggaaaa atttacaag 
      aaacttact tattgccga agcaataat tcaagttgc 
      atttaaacc aaatattgc aagtttatt atttcatat 
      tttgttatt aaattgttt aataatttt atcaacttt 
      tccttgtag attagattt tattttgaa attatcacg 
      ggaataaat attgctcat ttccacatt attggtact 
      taacgtgct acactcaca cacaaacta agtttgttg 
      cgctctctg ttgccaaaa aaaatatta gtaattacg 
      ctatatcgt aataaaaag tcatttttc gtaataata 
      ctaataaac atgacggta agttaaact tgtcattgt 
      cattacaat tcataaaaa tgtcaacat cacattttt 
      cagaactgt agaaaaaat ggcaacagc gcattaatt 
      ttttttggg gtcaaacga atcaccaat taaaatagt 
      ctgtgtttc cgtgtgatt ttgtgttaa ttggatttt 
      agtgacaaa tttacagcc aaggaaatt caccaatta 
      taagagtaa accaattgt gaaaagtta tttttatta 
      ttgtttttc aacggttta aaatcggct attcgaggg 
      cgcaaaaat cttagcttg aaattataa ttttacttt 
      agccatgct catgataag aactcaaca cctgttata 
      tcggtaaaa tcctaattt tattatttt tgtcaaaaa 
      tatgtcaaa tttgaattt tacagtccc ctagttctg 
      tcttaattt gcatcaatt tttctaaaa cctatacaa 
      taaataaag aaaatgccg ttaaaagcg acaatatga 
      tggaaaaac tgacatttt tttctggaa ttgtccgta 
      aaatagtac ttttcagca cactttatc tgtcaagtc 
      gtgtcaaaa atgactaaa aaagtgcat cgaatgcat 
      aatatttaa aaatgcaat gtgctaaaa ttacttttt 
      attacactg agtgcggct aaagtagta caaggtgtg 
      ttaaaatga ttttcatct agtcgctat gaatttctc 
      atactgacg gacattaat aataaaaaa ggtgttttc 
      ttgcacaat atttacttg cttcttaac caaacattt 
      gaatgcaat taattgact gttatgacg ttaaattca 
      tttctatct agatgagaa tcattttaa cacactttg 
      tattttacg agtaggaat atttaagtc ataatatta 
      ttgtgatgt aaaaaatgt aaaattatt tattttcac 
      tattacgta tagataatt ttattgaag ttaatttag 
      tacaaagag gtaacattc cttattgtg taatttttg 
      atctaataa atgaacctc aattacgaa ttgtttgct 
      tttgaaatc cccgcctta acttgacca atggcgttc 
      acctgtcac ttttccttg taattattt acctgctgt 
      catttttcg tgcaccaaa atattaaat gtttaccga 
      tgcaataaa aatgcacct ccgttatca atgtaacta 
      agataagat aaccgtcga gatgggttt cgagtcgga 
      ttccccgat ccacacagt ttggaagag tgacctgta 
      ccgtatgca gctggctgc tcccgtccc tattcccat 
      aatcagtga cgaggtgcc tcccgattt gcccagttt 
      tttgggccg cgactacca cggggctat cacgcacat 
      ccaggccca gaatctcct cagccccca tgaaaatgg 
      ggcttattt ggtgcgcag cagtcgctc ggccaacgg 
      ccagtttca caccttgag tctcaaatt taacgataa 
      aattcatca ttacaaact tttctatga cccaaattc 
      ggggcttta tgttaggga aaaacgcta tgactgtgt 
      caggtcgtt agtggctga tggcttagt cacaatgta 
      tctcgaact taaggcacc tttaatttt gcaaagact 
      tgccaccgc taattatca agagagtcc ttacatgac 
      gttaaataa gcgcaagtt gagggcttt aagcaaaga 
      acatgccaa aaataaaaa ttacgtgaa agattcaaa 
      cattccgcc aaatattcc attaagtca gcagatatt 
      agggcccaa ttttccaga gaaatatga aaaacaaca 
      cgcgtttaa attgacaac gtttaaggg gcttaattg 
      gtgtgaatt gtgtgggaa ttttttgtg gggctttac 
      gtttcaagg aaagaaatg tgatgattg tggaatgat 
      agctcatgt tcgctgttc tgagaaatt ccccaacga 
      ttgtgtccc cgacttgaa atatcttcg tggagtgtt 
      tggtattga actgaccac acttttaac agcccacaa 
      tgccaaatt accatttgt ggtaacgaa atgtgtggc 
      tgaagttga agctagagg acttacaac ggagggaat 
      ttatcgttt gagtggttt tgctgaaga aattgaagc 
      gattaaaat ggctttcga taaagatgg cgataaagc 
      cgatttaag tcaggaaaa atacccaaa tgttaatgt 
      aataacagg ggcgttaaa atcatatct tagactttt 
      accaattcc gcttattac atttatcgt tcatccact 
      tctcattga cgccatgca acataaaaa tcacgattt 
      gaaaatgtc gtcagttaa gcacgccct taaatcatt 
      gccgaaagc acattatga tactttgaa atacatgat 
      tgagcattt acacagggt gtctttaca ttcggcaat 
      aaataaaat gaattctca caacttggc aactgtttt 
      tgccccaac tctgattgg accaagcga atctgcaag 
      cgacgtaat gcctgatat gacaagtga tattttatt 
      aattgaaac tctcatcac acattgtga acgtatttt 
      taaaaatta gcgactaaa agacaacaa tgctctctg 
      gtttaaaaa tttttcgtt gtgttttcg gtgcaaaat 
      caattgtga ttatttttg tgatataca aggcaatat 
      taagtatta tcggtaagg tactagtgt ttctaccat 
      ttgtaatct cacgtactc tattatttt
    • >TC009490 RB
      tttgcttca tgataaaaa tgcaatata ttgccaatc 
      gataagtcc acacgtgtt tattattgc atacactat 
      atttattta tacataaag cacaaaaag cacgcctat 
      gataaaagg aagttggac gcaccttta aggagcaag 
      aaacgataa caagccaag ttactgcca aacattatt 
      ccaaatatt tctcaggaa tgtggtaag agtgtcagt 
      ggaataaaa ttaggccaa aggagataa gacccaaac 
      tgcgttcac ctatattat gttataagt gtataatgt 
      agttttagg cagaataga gcaggaaaa atttacaag 
      aaacttact tattgccga agcaataat tcaagttgc 
      atttaaacc aaatattgc aagtttatt atttcatat 
      tttgttatt aaattgttt aataatttt atcaacttt 
      tccttgtag attagattt tattttgaa attatcacg 
      ggaataaat attgctcat ttccacatt attggtact 
      taacgtgct acactcaca cacaaacta agtttgttg 
      cgctctctg ttgccaaaa aaaatatta gtaattacg 
      ctatatcgt aataaaaag tcatttttc gtaataata 
      ctaataaac atgacggta agttaaact tgtcattgt 
      cattacaat tcataaaaa tgtcaacat cacattttt 
      cagaactgt agaaaaaat ggcaacagc gcattaatt 
      ttttttggg gtcaaacga atcaccaat taaaatagt 
      ctgtgtttc cgtgtgatt ttgtgttaa ttggatttt 
      agtgacaaa tttacagcc aaggaaatt caccaatta 
      taagagtaa accaattgt gaaaagtta tttttatta 
      ttgtttttc aacggttta aaatcggct attcgaggg 
      cgcaaaaat cttagcttg aaattataa ttttacttt 
      agccatgct catgataag aactcaaca cctgttata 
      tcggtaaaa tcctaattt tattatttt tgtcaaaaa 
      tatgtcaaa tttgaattt tacagtccc ctagttctg 
      tcttaattt gcatcaatt tttctaaaa cctatacaa 
      taaataaag aaaatgccg ttaaaagcg acaatatga 
      tggaaaaac tgacatttt tttctggaa ttgtccgta 
      aaatagtac ttttcagca cactttatc tgtcaagtc 
      gtgtcaaaa atgactaaa aaagtgcat cgaatgcat 
      aatatttaa aaatgcaat gtgctaaaa ttacttttt 
      attacactg agtgcggct aaagtagta caaggtgtg 
      ttaaaatga ttttcatct agtcgctat gaatttctc 
      atactgacg gacattaat aataaaaaa ggtgttttc 
      ttgcacaat atttacttg cttcttaac caaacattt 
      gaatgcaat taattgact gttatgacg ttaaattca 
      tttctatct agatgagaa tcattttaa cacactttg 
      tattttacg agtaggaat atttaagtc ataatatta 
      ttgtgatgt aaaaaatgt aaaattatt tattttcac 
      tattacgta tagataatt ttattgaag ttaatttag 
      tacaaagag gtaacattc cttattgtg taatttttg 
      atctaataa atgaacctc aattacgaa ttgtttgct 
      tttgaaatc cccgcctta acttgacca atggcgttc 
      acctgtcac ttttccttg taattattt acctgctgt 
      catttttcg tgcaccaaa atattaaat gtttaccga 
      tgcaataaa aatgcacct ccgttatca atgtaacta 
      agataagat aaccgtcga gatgggttt cgagtcgga 
      ttccccgat ccacacagt ttggaagag tgaccgtaa 
      gtgctatgc tatcgtaac ctaacttaa ctcaataaa 
      atggtcggt gttggaatt ttcaattta aataaacta 
      gacatggag gcgtgttgc aactacttg taccctccg 
      tctttcgat ttcttgcgc ttgtaccgt atgcagctg 
      gctgctccc gtccctatt cccataatc agtgacgag 
      gtgcctccc gatttgccc agttttttg ggccgcgac 
      taccacggg gctatcacg cacatccag gcccagaat 
      ctcctcagc ccccatgaa aatggggct tatttggtg 
      cgcagcagt cgctcggcc aacggccag tttcacacc 
      ttgagtctc aaatttaac gataaaatt catcattac 
      aaacttttc tatgaccca aattcgggg ctttatgtt 
      agggaaaaa cgctatgac tgtgtcagg tcgttagtg 
      gctgatggc ttagtcaca atgtatctc gaacttaag 
      gcaccttta attttgcaa agacttgcc accgctaat 
      tatcaagag agtccttac atgacgtta aataagcgc 
      aagttgagg gctttaagc aaagaacat gccaaaaat 
      aaaaattac gtgaaagat tcaaacatt ccgccaaat 
      attccatta agtcagcag atattaggg cccaatttt 
      ccagagaaa tatgaaaaa caacacgcg tttaaattg 
      acaacgttt aaggggctt aattggtgt gaattgtgt 
      gggaatttt ttgtggggc tttacgttt caaggaaag 
      aaatgtgat gattgtgga atgatagct catgttcgc 
      tgttctgag aaattcccc aacgattgt gtccccgac 
      ttgaaatat cttcgtgga gtgtttggt attgaactg 
      accacactt ttaacagcc cacaatgcc aaattacca 
      tttgtggta acgaaatgt gtggctgaa gttgaagct 
      agaggactt acaacggag ggaatttat cgtttgagt 
      ggttttgct gaagaaatt gaagcgatt aaaatggct 
      ttcgataaa gatggcgat aaagccgat ttaagtcag 
      gaaaaatac ccaaatgtt aatgtaata acaggggcg 
      ttaaaatca tatcttaga cttttacca attccgctt 
      attacattt atcgttcat ccacttctc attgacgcc 
      atgcaacat aaaaatcac gatttgaaa atgtcgtca 
      gttaagcac gcccttaaa tcattgccg aaagcacat 
      tatgatact ttgaaatac atgattgag catttacac 
      agggtgtct ttacattcg gcaataaat aaaatgaat 
      tctcacaac ttggcaact gtttttgcc ccaactctg 
      attggacca agcgaatct gcaagcgac gtaatgcct 
      gatatgaca agtgatatt ttattaatt gaaactctc 
      atcacacat tgtgaacgt atttttaaa aattagcga 
      ctaaaagac aacaatgct ctctggttt aaaaatttt 
      tcgttgtgt tttcggtgc aaaatcaat tgtgattat 
      ttttgtgat atacaaggc aatattaag tattatcgg 
      taaggtact agtgtttct accatttgt aatctcacg 
      tactctatt atttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY