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Gene information
General information
TC009322 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 383326..385753
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009322
    iBeetle screen iB_08615

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009322 RA
      MSVFLTCRL KNSIGRKKF FNFSSDIVR QLSQQVKQN 
      ESFVPFMDV IKFRREEAR RSILVQVQS AQSFKELHS 
      YCNSVGNVR QMLHYTVGV EPLNFIVVE FASEKDVTN 
      VLERSSYLE DSQVVPVQS QFLWFRASN RKLPKLKQS 
      KSAILSIEN GNQCVTPSE IVNLLSKCD SVSEQMNVL 
      YNSTKLNDL GTRLRFLTA RQVENAVRG MFPKAKAYP 
      FGSSVNGYG KMGCDLDLV LRLCDDKVK NDARLMFHC 
      KGLVGSERT ASQRNMEAI GDLLQLFLP GCSQVRRIL 
      QARVPIIKY YQQLTDVEC DLSMANMSG VHMSDFLYI 
      MGSLDARIR PLVFTIRKW ASEIGLTNS SPGRWITNF 
      SLTLLVLAF LQKPINSKP ILPSLNTLV KLAEPKDSY 
      MTEDGINCT FLRDITKLK TPTENKESL ETLLVEFFE 
      FYSQFDFAS KALCLNESV AITKPEHCA LYIVNPLER 
      GLNVSKNVS MEELDRFRV EARNAAWIL ESQENKNEN 
      WGILSIFEN KRKASSVVN FAFANKHGR LVEVSTLFE 
      ENTDKPVEY KNASVKKQV ENIKNDTKN KLKELQKTN 
      R
    CDS sequence
    • >TC009322 RA
      atgtcggtt tttttgact tgccgtttg aaaaattca 
      attgggcga aagaaattc ttcaatttc agtagtgat 
      attgtacgc caattgtct caacaagtg aagcaaaat 
      gagtcgttt gtgcccttt atggacgtt ataaaattt 
      cggcgagag gaggccaga cgcagtatt ttagtgcaa 
      gtccaatcg gctcagtcc tttaaagaa ctccactcg 
      tattgcaat tctgtgggg aatgttagg caaatgttg 
      cactacaca gttggggtt gaaccactg aatttcatt 
      gtggtggaa tttgcatcc gaaaaggac gtaaccaat 
      gttcttgag cgcagttct tatctggaa gacagtcaa 
      gtggttcca gtccagtca caatttttg tggtttagg 
      gcttcaaat cgtaaatta ccaaaatta aagcagtcc 
      aaaagtgcc attttaagc attgaaaat ggtaatcag 
      tgtgtgaca ccttctgaa atagtgaat ttgttgagc 
      aaatgtgat tcagtgtct gagcaaatg aatgttctt 
      tacaattca accaaattg aacgatctt gggacgaga 
      ttacgtttt ttaactgca agacaagtc gaaaatgct 
      gttaggggg atgtttcca aaggctaag gcttatcca 
      tttggctcc tcggtcaat gggtatgga aaaatgggt 
      tgtgaccta gacctagtc ttacgatta tgtgacgat 
      aaagtaaaa aatgatgcc agattaatg tttcactgt 
      aagggttta gtagggagt gaaagaacc gcttcacaa 
      cgaaatatg gaagcaatc ggtgattta ttgcagtta 
      tttttgcca gggtgttca caagtccgc cgaatattg 
      caagccaga gtccctata ataaaatat taccagcaa 
      ctgactgac gttgagtgt gatctttca atggcaaac 
      atgtctgga gtccacatg tccgacttc ctgtacatc 
      atggggtcc ctcgacgcg cgcattcgc ccccttgtt 
      ttcacaatt cgcaaatgg gcctccgag atcggctta 
      acaaactca agccccggt cgttggatc acaaacttc 
      tccctaact ctgctagtc ctcgcattt ttgcaaaaa 
      ccgatcaat tccaaacca attcttcca tcactgaac 
      actttagtc aaactggcc gaacccaaa gactcttac 
      atgactgaa gatggaata aattgcact ttccttcga 
      gacattaca aagctcaaa accccgacc gaaaataag 
      gaaagtttg gaaacactt cttgttgag tttttcgaa 
      ttttattcg cagtttgat ttcgcgtcg aaagctttg 
      tgtttgaat gaatcggtc gcgataaca aaacccgaa 
      cattgcgct ctgtatatt gtgaatccg ttagaaagg 
      gggctcaat gttagtaaa aatgtcagt atggaagaa 
      ctggaccgg tttcgagtt gaggcgaga aacgctgct 
      tggatttta gaatcacag gagaacaag aatgaaaat 
      tggggcatt ttatcaatt ttcgaaaat aaacgcaag 
      gctagcagt gttgtgaat ttcgcattt gcaaataaa 
      cacggaagg ttggtggaa gtgagcact ttatttgag 
      gaaaatacc gacaaacct gttgagtat aaaaacgcc 
      tctgtgaag aaacaagtg gagaacata aaaaacgac 
      acgaaaaat aaactgaaa gaactacaa aaaacaaac 
      aggtga
    mRNA sequence
    • >TC009322 RA
      aatgtcggt ttttttgac ttgccgttt gaaaaattc 
      aattgggcg aaagaaatt cttcaattt cagtagtga 
      tattgtacg ccaattgtc tcaacaagt gaagcaaaa 
      tgagtcgtt tgtgccctt tatggacgt tataaaatt 
      tcggcgaga ggaggccag acgcagtat tttagtgca 
      agtccaatc ggctcagtc ctttaaaga actccactc 
      gtattgcaa ttctgtggg gaatgttag gcaaatgtt 
      gcactacac agttggggt tgaaccact gaatttcat 
      tgtggtgga atttgcatc cgaaaagga cgtaaccaa 
      tgttcttga gcgcagttc ttatctgga agacagtca 
      agtggttcc agtccagtc acaattttt gtggtttag 
      ggcttcaaa tcgtaaatt accaaaatt aaagcagtc 
      caaaagtgc cattttaag cattgaaaa tggtaatca 
      gtgtgtgac accttctga aatagtgaa tttgttgag 
      caaatgtga ttcagtgtc tgagcaaat gaatgttct 
      ttacaattc aaccaaatt gaacgatct tgggacgag 
      attacgttt tttaactgc aagacaagt cgaaaatgc 
      tgttagggg gatgtttcc aaaggctaa ggcttatcc 
      atttggctc ctcggtcaa tgggtatgg aaaaatggg 
      ttgtgacct agacctagt cttacgatt atgtgacga 
      taaagtaaa aaatgatgc cagattaat gtttcactg 
      taagggttt agtagggag tgaaagaac cgcttcaca 
      acgaaatat ggaagcaat cggtgattt attgcagtt 
      atttttgcc agggtgttc acaagtccg ccgaatatt 
      gcaagccag agtccctat aataaaata ttaccagca 
      actgactga cgttgagtg tgatctttc aatggcaaa 
      catgtctgg agtccacat gtccgactt cctgtacat 
      catggggtc cctcgacgc gcgcattcg cccccttgt 
      tttcacaat tcgcaaatg ggcctccga gatcggctt 
      aacaaactc aagccccgg tcgttggat cacaaactt 
      ctccctaac tctgctagt cctcgcatt tttgcaaaa 
      accgatcaa ttccaaacc aattcttcc atcactgaa 
      cactttagt caaactggc cgaacccaa agactctta 
      catgactga agatggaat aaattgcac tttccttcg 
      agacattac aaagctcaa aaccccgac cgaaaataa 
      ggaaagttt ggaaacact tcttgttga gtttttcga 
      attttattc gcagtttga tttcgcgtc gaaagcttt 
      gtgtttgaa tgaatcggt cgcgataac aaaacccga 
      acattgcgc tctgtatat tgtgaatcc gttagaaag 
      ggggctcaa tgttagtaa aaatgtcag tatggaaga 
      actggaccg gtttcgagt tgaggcgag aaacgctgc 
      ttggatttt agaatcaca ggagaacaa gaatgaaaa 
      ttggggcat tttatcaat tttcgaaaa taaacgcaa 
      ggctagcag tgttgtgaa tttcgcatt tgcaaataa 
      acacggaag gttggtgga agtgagcac tttatttga 
      ggaaaatac cgacaaacc tgttgagta taaaaacgc 
      ctctgtgaa gaaacaagt ggagaacat aaaaaacga 
      cacgaaaaa taaactgaa agaactaca aaaaacaaa 
      caggtgact ttgtaacaa tgtatttaa gtattatgt 
      atacatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY