Status
Please wait ...
Gene information
General information
TC033521 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1123348..1129014
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033521
    iBeetle screen iB_08598

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033521 RA
      MLKFYARFE ISDETGDPL TDHDMTQLH YSKITSLQK 
      AAFAKFPDL RSFSLANVA SVDTRKALI KHFGPLNKD 
      KLRLIATYL KLVPSPEEE TEVNWCRLD EEFLRELLV 
      SRHERRTSQ LEALNEMPL YPTEEVIWD ENVVPTAYF 
      SGEGCLALP KLNLQFLTL HDYLLRNFN LFRLESTYE 
      IRQDIEDAV SRLCPWRAE DDSVYWGGW ARMAQPILN 
      FAVVEVAKP NIGEKRPSR VRADVTVNL SVRPEIKSE 
      WENLRKHDV CFLITVRPP NSIGTKYDY KQPFIPQVG 
      LHCVRGCEI EGMLDTNGR VIEDGPELR PNIPGDKRT 
      YRVWLDCNQ YRDDMNNVN QGKEDVYEE FNILMRRKP 
      KENNFKAVL ETIRELMNT ECVVPDWLH DIILGYGDP 
      SAAHYAKMP SPTTTMDFN DTFIDMDHL RSCFPDYQI 
      RVKTNDPKK LVRPFRLTF GDLGTGSEK VITVEPYVT 
      PKRGPYHFN EPKKNSIPF TPTQIEAIK SGMQPGLTL 
      VVGPPGTGK TDVAVQIIS NIYHNFPNQ RTLIVTHSN 
      QALNQLFEK IVALDIDER HLLRLGHGE EALETEKDF 
      SRYGRVNYV LAKRLDLLV EVQKLQESL DVKGDVAYT 
      CETAGHFYL YQILSRWEN YQSIVKPKR QQDVPIEKV 
      NAEFPFHKF FADAQPLFK GKSFDEDLE IAESCFRYI 
      EHIFKELEE FRAFELLRS GLDRSKYLL VKEAKIIAM 
      TCTHAALKR KELVEMGFK YDNILMEEA AQILEIETF 
      IPLLLQNPQ DGYNRLKRW IMIGDHHQL PPVIKNMAF 
      QKYSNMEQS LFTRLVRLG VPTVDLDGQ GRARPNICN 
      LYKWRYKNL GNLAHVLNW AEYRTANAG FAFDFQLID 
      TQDFNGVGE SEPSPYFYQ NLAEAEYCV AVFMYMRLI 
      GYPAHKITI LTTYNGQKH LIRDVINAR CANNPLIGR 
      PHKVTTVDK YQGQQNDFI LLSLVRTKA VGHLRDVRR 
      LVVAMSRAR LGLYVFARV ALFQNCFEL TPAFEQLTQ 
      RPTKLHLVV NETYPAKRY NDEKPQEVV VSQDMTYMA 
      NFVYTFYTQ RVKQMKEYY EQTQKWMKP GACEVKEHE 
      YFVSSHPGE DRDTDSEDE REEEEETVQ LPVPIEDEP 
      IEAETIDSE MPEEEDERS EVRDTGEVE NDE
    CDS sequence
    • >TC033521 RA
      atgctgaaa ttttacgca agattcgaa atcagcgac 
      gaaacggga gatccctta accgaccac gacatgaca 
      cagttacac tactcaaaa attacctca cttcagaaa 
      gcagcgttt gccaaattt cccgatttg cgtagtttt 
      tctttggcc aacgttgcg agtgtggac acgcgcaag 
      gctctaatc aaacatttc ggtccctta aacaaagat 
      aagttgaga ctgatcgca acgtattta aagttggtg 
      ccgtctccc gaggaggaa acggaggtt aattggtgc 
      cgattagat gaggagttt ttgcgcgaa cttttggtg 
      tctagacat gaacgaagg acatcgcag ttggaggct 
      ttaaatgaa atgccgctt tatccaacc gaggaagtg 
      atctgggat gagaacgtg gtaccaact gcttatttt 
      tcaggggaa ggctgtctg gctttgccc aagctgaat 
      ctacagttc ctgacactt cacgattat cttttgagg 
      aactttaat ctgtttcgc ttggaatcg acttacgaa 
      attcggcaa gacatcgaa gatgcagtc agtcggctg 
      tgcccttgg cgggctgaa gacgacagc gtttattgg 
      ggcggttgg gcccgcatg gctcaaccg atcctaaat 
      ttcgccgtg gtagaagtg gcaaaacca aacattggt 
      gaaaaaagg ccaagtcgc gtgcgcgcc gacgtcaca 
      gttaatctt tccgtacgt ccggaaata aaatccgag 
      tgggaaaat ttacgcaaa cacgacgtc tgtttcctg 
      ataactgtg cgcccacca aactcaatc ggcacaaaa 
      tacgactat aaacaaccc tttatacca caagtcggc 
      ctccattgc gtgcgaggc tgcgagatt gagggaatg 
      ttggataca aacggccga gtgattgaa gatgggcct 
      gaactccgt ccgaacatc ccgggcgat aaacggacg 
      tatcgcgtt tggttggat tgtaaccag tatcgggac 
      gacatgaac aatgttaac caaggcaag gaggacgtc 
      tacgaagag tttaacatt ttgatgcgt cgcaagccc 
      aaagaaaac aattttaag gcagtgctt gaaacgatc 
      cgggagttg atgaatacg gaatgtgtt gttccggat 
      tggttgcat gacattatt ttgggctac ggtgacccg 
      agtgctgca cattacgcc aaaatgccc agcccgacc 
      actactatg gattttaac gatactttc atagacatg 
      gatcatttg cgttcgtgt ttccccgat tatcaaatc 
      agggtgaaa acgaacgat ccgaaaaaa ttagttcgg 
      ccttttcgc ctaactttt ggtgatttg ggcaccggt 
      agtgagaaa gtaatcaca gttgaaccg tacgttaca 
      ccaaaacgt ggcccttat cattttaac gaacccaaa 
      aagaattcc atcccgttc actccgact caaatcgag 
      gcgattaaa tcgggcatg cagcccggt ttaaccctc 
      gtggtgggg cccccaggt accggcaag accgacgtt 
      gctgtacag ataatctcc aatatttat cacaatttt 
      cccaatcag cgcaccctc attgttacg cactcgaat 
      caggcgctt aatcagctc tttgagaaa attgtggcg 
      ttggatatc gacgagcga catttgttg cggcttggg 
      cacggcgag gaagctctc gagactgag aaggatttc 
      agtcgttat ggacgtgtt aactatgtg ttagctaag 
      cggttggat ttgttggtt gaggtgcaa aaattgcaa 
      gaatcgctc gatgtgaaa ggcgatgtt gcatatacg 
      tgtgaaacc gccgggcat ttttatctc tatcagatt 
      ttgtcgagg tgggagaat tatcaaagt attgttaag 
      ccgaaacgg caacaggat gtgccgatt gagaaagtt 
      aatgccgag tttccgttt cataagttt ttcgctgat 
      gcgcaaccg cttttcaaa gggaagagc tttgatgag 
      gatttggaa attgccgaa agttgcttc agatacatt 
      gagcacatt ttcaaagaa ctggaagaa ttccgcgcc 
      tttgaatta ctgcgctca ggcctagac cgttccaaa 
      tatttgctc gtaaaagag gcgaaaata atcgcaatg 
      acttgcacc cacgctgct ttaaaacgc aaagaactg 
      gtggaaatg ggtttcaaa tacgataat attctcatg 
      gaagaagcg gcccaaatc ctcgaaatc gaaaccttt 
      attccactc ctcctccaa aacccacaa gacggttat 
      aaccgcttg aaacgttgg ataatgata ggggaccac 
      caccagttg cccccagtc atcaaaaac atggccttc 
      cagaaatac tccaacatg gagcaaagt ctcttcacc 
      cgcttggtt cgcttaggc gtcccgact gtagattta 
      gatggacaa gggcgtgcc agacccaat atttgcaac 
      ttgtacaag tggcgttac aaaaacctg ggtaatttg 
      gctcatgtg ttgaactgg gcggaatat cgcactgca 
      aatgctggg tttgcgttc gatttccaa ctgattgat 
      actcaggat tttaacggg gtgggggag tcggagcct 
      agtccttat ttttaccag aatctggca gaggctgag 
      tattgtgtt gccgttttc atgtatatg agactgatt 
      ggttatccg gcgcataaa ataactatt ttgacgacg 
      tacaacggg caaaaacac ttgatacgg gacgttatc 
      aatgcaagg tgtgccaat aacccgttg attgggagg 
      ccacataaa gtcacaacg gttgataag tatcaaggg 
      caacaaaac gattttatt ttgttgtcg ctggtgagg 
      actaaagct gtggggcat ttgcgggat gtgaggcga 
      ttggttgtg gcgatgtca cgtgccagg ctggggttg 
      tatgttttt gcgagagtt gccctcttt cagaattgt 
      tttgagttg actcctgcc tttgaacag ttgactcaa 
      agaccgact aagttgcat ttggtggtc aatgagacg 
      tatcctgcc aagcggtac aatgacgag aaaccgcag 
      gaagtggta gtgagtcag gatatgact tatatggcc 
      aattttgtc tacacgttt tatacacaa agggttaag 
      caaatgaag gagtattat gagcaaacg caaaagtgg 
      atgaaacct ggagcttgt gaggtcaaa gaacacgaa 
      tatttcgtg tcttcgcat ccgggtgaa gaccgcgat 
      actgacagt gaagatgag agggaagaa gaggaagaa 
      actgtgcag ttgcctgtg ccaattgag gatgagccg 
      attgaagca gagacgatt gattccgaa atgcccgaa 
      gaagaggac gagaggagc gaagttcgg gatacgggc 
      gaagtggag aatgatgaa taa
    mRNA sequence
    • >TC033521 RA
      cggaataca ataaattct taccttaaa tgcgtacga 
      tgagtaact gttgattag agagcgcta ttcaaaatg 
      ataattcta ttttaataa ccacatcac gtttctgca 
      cgcttatga ccgaaaagt gttttagcc agaggttta 
      gcggaattt gaagcgatc ttccagcta actcttttc 
      ccacccaga cgacacaac atagtagta attttgaaa 
      atgctctta gacatgctg aaattttac gcaagattc 
      gaaatcagc gacgaaacg ggagatccc ttaaccgac 
      cacgacatg acacagtta cactactca aaaattacc 
      tcacttcag aaagcagcg tttgccaaa tttcccgat 
      ttgcgtagt ttttctttg gccaacgtt gcgagtgtg 
      gacacgcgc aaggctcta atcaaacat ttcggtccc 
      ttaaacaaa gataagttg agactgatc gcaacgtat 
      ttaaagttg gtgccgtct cccgaggag gaaacggag 
      gttaattgg tgccgatta gatgaggag tttttgcgc 
      gaacttttg gtgtctaga catgaacga aggacatcg 
      cagttggag gctttaaat gaaatgccg ctttatcca 
      accgaggaa gtgatctgg gatgagaac gtggtacca 
      actgcttat ttttcaggg gaaggctgt ctggctttg 
      cccaagctg aatctacag ttcctgaca cttcacgat 
      tatcttttg aggaacttt aatctgttt cgcttggaa 
      tcgacttac gaaattcgg caagacatc gaagatgca 
      gtcagtcgg ctgtgccct tggcgggct gaagacgac 
      agcgtttat tggggcggt tgggcccgc atggctcaa 
      ccgatccta aatttcgcc gtggtagaa gtggcaaaa 
      ccaaacatt ggtgaaaaa aggccaagt cgcgtgcgc 
      gccgacgtc acagttaat ctttccgta cgtccggaa 
      ataaaatcc gagtgggaa aatttacgc aaacacgac 
      gtctgtttc ctgataact gtgcgccca ccaaactca 
      atcggcaca aaatacgac tataaacaa ccctttata 
      ccacaagtc ggcctccat tgcgtgcga ggctgcgag 
      attgaggga atgttggat acaaacggc cgagtgatt 
      gaagatggg cctgaactc cgtccgaac atcccgggc 
      gataaacgg acgtatcgc gtttggttg gattgtaac 
      cagtatcgg gacgacatg aacaatgtt aaccaaggc 
      aaggaggac gtctacgaa gagtttaac attttgatg 
      cgtcgcaag cccaaagaa aacaatttt aaggcagtg 
      cttgaaacg atccgggag ttgatgaat acggaatgt 
      gttgttccg gattggttg catgacatt attttgggc 
      tacggtgac ccgagtgct gcacattac gccaaaatg 
      cccagcccg accactact atggatttt aacgatact 
      ttcatagac atggatcat ttgcgttcg tgtttcccc 
      gattatcaa atcagggtg aaaacgaac gatccgaaa 
      aaattagtt cggcctttt cgcctaact tttggtgat 
      ttgggcacc ggtagtgag aaagtaatc acagttgaa 
      ccgtacgtt acaccaaaa cgtggccct tatcatttt 
      aacgaaccc aaaaagaat tccatcccg ttcactccg 
      actcaaatc gaggcgatt aaatcgggc atgcagccc 
      ggtttaacc ctcgtggtg gggccccca ggtaccggc 
      aagaccgac gttgctgta cagataatc tccaatatt 
      tatcacaat tttcccaat cagcgcacc ctcattgtt 
      acgcactcg aatcaggcg cttaatcag ctctttgag 
      aaaattgtg gcgttggat atcgacgag cgacatttg 
      ttgcggctt gggcacggc gaggaagct ctcgagact 
      gagaaggat ttcagtcgt tatggacgt gttaactat 
      gtgttagct aagcggttg gatttgttg gttgaggtg 
      caaaaattg caagaatcg ctcgatgtg aaaggcgat 
      gttgcatat acgtgtgaa accgccggg catttttat 
      ctctatcag attttgtcg aggtgggag aattatcaa 
      agtattgtt aagccgaaa cggcaacag gatgtgccg 
      attgagaaa gttaatgcc gagtttccg tttcataag 
      tttttcgct gatgcgcaa ccgcttttc aaagggaag 
      agctttgat gaggatttg gaaattgcc gaaagttgc 
      ttcagatac attgagcac attttcaaa gaactggaa 
      gaattccgc gcctttgaa ttactgcgc tcaggccta 
      gaccgttcc aaatatttg ctcgtaaaa gaggcgaaa 
      ataatcgca atgacttgc acccacgct gctttaaaa 
      cgcaaagaa ctggtggaa atgggtttc aaatacgat 
      aatattctc atggaagaa gcggcccaa atcctcgaa 
      atcgaaacc tttattcca ctcctcctc caaaaccca 
      caagacggt tataaccgc ttgaaacgt tggataatg 
      ataggggac caccaccag ttgccccca gtcatcaaa 
      aacatggcc ttccagaaa tactccaac atggagcaa 
      agtctcttc acccgcttg gttcgctta ggcgtcccg 
      actgtagat ttagatgga caagggcgt gccagaccc 
      aatatttgc aacttgtac aagtggcgt tacaaaaac 
      ctgggtaat ttggctcat gtgttgaac tgggcggaa 
      tatcgcact gcaaatgct gggtttgcg ttcgatttc 
      caactgatt gatactcag gattttaac ggggtgggg 
      gagtcggag cctagtcct tatttttac cagaatctg 
      gcagaggct gagtattgt gttgccgtt ttcatgtat 
      atgagactg attggttat ccggcgcat aaaataact 
      attttgacg acgtacaac gggcaaaaa cacttgata 
      cgggacgtt atcaatgca aggtgtgcc aataacccg 
      ttgattggg aggccacat aaagtcaca acggttgat 
      aagtatcaa gggcaacaa aacgatttt attttgttg 
      tcgctggtg aggactaaa gctgtgggg catttgcgg 
      gatgtgagg cgattggtt gtggcgatg tcacgtgcc 
      aggctgggg ttgtatgtt tttgcgaga gttgccctc 
      tttcagaat tgttttgag ttgactcct gcctttgaa 
      cagttgact caaagaccg actaagttg catttggtg 
      gtcaatgag acgtatcct gccaagcgg tacaatgac 
      gagaaaccg caggaagtg gtagtgagt caggatatg 
      acttatatg gccaatttt gtctacacg ttttataca 
      caaagggtt aagcaaatg aaggagtat tatgagcaa 
      acgcaaaag tggatgaaa cctggagct tgtgaggtc 
      aaagaacac gaatatttc gtgtcttcg catccgggt 
      gaagaccgc gatactgac agtgaagat gagagggaa 
      gaagaggaa gaaactgtg cagttgcct gtgccaatt 
      gaggatgag ccgattgaa gcagagacg attgattcc 
      gaaatgccc gaagaagag gacgagagg agcgaagtt 
      cgggatacg ggcgaagtg gagaatgat gaataattt 
      aataaaaaa atttaatga ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY