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Gene information
General information
TC031360
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012943 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18939654..19000378
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031360
    iBeetle screen iB_08595

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031360 RA
      MCDVRRRTW VLLGLLLGL FTGGDALKD 
VSVTIPPAV KIMDTVTLQ CRYDLQGEP 
LYTVKWYKG GQEFFRYIP KELPSTQVF 
PLPGINVDL SKSSPNEVV LRNVQPEVT 
GRYKCEVSS DAPNFYTFI VSGYMYVIG 
LILGSTAIP RFTARTNRH KPRFKLIRT 
NNIILHYET NSIYTYFKI ASLMRASPE 
LTFFKDFTL
    • >TC031360 RB
      MCDVRRRTW VLLGLLLGL FTGGDALKD 
VSVTIPPAV KIMDTVTLQ CRYDLQGEP 
LYTVKWYKG GQEFFRYIP KELPSTQVF 
PLPGINVDL SKSSPNEVV LRNVQPEVT 
GRYKCEVSS DAPNFYTFI VSGYMYVIG 
LTAIHILRA GQLHEDVTY FKIASLMRA 
SPELTFFKD FTL
    CDS sequence
    • >TC031360 RA
      atgtgcgac gtccgacgc aggacttgg gtcttgttg 
gggttgctg ctgggattg tttaccggt ggggacgct 
ctcaaggac gtatcagtg accattcct cccgccgtc 
aaaatcatg gacaccgtc acgctgcag tgccggtac 
gacctccaa ggggagccc ctctacacc gtcaagtgg 
tacaagggc ggccaggag ttcttccgg tacatcccc 
aaggagctg ccaagcacg caggtgttc cctctgccg 
gggatcaac gtcgatctg agcaagtcc agccctaac 
gaggtcgtc ctgcgaaat gtgcagccg gaagtcacc 
ggacgatac aaatgcgag gtttcctca gacgcacca 
aatttctac actttcata gtctcgggg tacatgtac 
gtgatcgga ctgatactc gggagcacc gcaattcca 
cgatttacg gccaggaca aatcgccac aaaccgaga 
tttaaatta attcgaacc aacaatatt attttgcat 
tatgaaaca aattcaatc tatacatat tttaaaatc 
gcttcgctc atgagggct tcgccggaa ttaacattt 
ttcaaagat tttactcta tag
    • >TC031360 RB
      atgtgcgac gtccgacgc aggacttgg gtcttgttg 
gggttgctg ctgggattg tttaccggt ggggacgct 
ctcaaggac gtatcagtg accattcct cccgccgtc 
aaaatcatg gacaccgtc acgctgcag tgccggtac 
gacctccaa ggggagccc ctctacacc gtcaagtgg 
tacaagggc ggccaggag ttcttccgg tacatcccc 
aaggagctg ccaagcacg caggtgttc cctctgccg 
gggatcaac gtcgatctg agcaagtcc agccctaac 
gaggtcgtc ctgcgaaat gtgcagccg gaagtcacc 
ggacgatac aaatgcgag gtttcctca gacgcacca 
aatttctac actttcata gtctcgggg tacatgtac 
gtgatcggt cttactgcc atccatatc cttagagct 
ggtcagtta catgaggat gttacatat tttaaaatc 
gcttcgctc atgagggct tcgccggaa ttaacattt 
ttcaaagat tttactcta tag
    mRNA sequence
    • >TC031360 RA
      tcagccccc aagaaacac acgcatttt attgaaaaa 
ggagccgcc gccgggggc gagatttca gttcggcga 
cggcgacgg aggtaggag tggcgtcgc tcttgcgtc 
tgtggagat gtgcgcggc gcagcttca tcaacggtg 
tctctctcc agtttccgc gcgctcttc caaatagca 
ggacgtcga acacaactg gccaaaagc ggcttttcc 
aacttttta gtgacactt tttccctct cggtgcaaa 
aaatgcgag cgtcttgtc ggcgtcgag gcgcttcgt 
gaagtggtg cgtgtgtgt ggtgagctc aagaagcga 
caaaatgtg cgacgtccg acgcaggac ttgggtctt 
gttggggtt gctgctggg attgtttac cggtgggga 
cgctctcaa ggacgtatc agtgaccat tcctcccgc 
cgtcaaaat catggacac cgtcacgct gcagtgccg 
gtacgacct ccaagggga gcccctcta caccgtcaa 
gtggtacaa gggcggcca ggagttctt ccggtacat 
ccccaagga gctgccaag cacgcaggt gttccctct 
gccggggat caacgtcga tctgagcaa gtccagccc 
taacgaggt cgtcctgcg aaatgtgca gccggaagt 
caccggacg atacaaatg cgaggtttc ctcagacgc 
accaaattt ctacacttt catagtctc ggggtacat 
gtacgtgat cggactgat actcgggag caccgcaat 
tccacgatt tacggccag gacaaatcg ccacaaacc 
gagatttaa attaattcg aaccaacaa tattatttt 
gcattatga aacaaattc aatctatac atattttaa 
aatcgcttc gctcatgag ggcttcgcc ggaattaac 
atttttcaa agattttac tctatagga taattatat 
aactactta tatcaaaaa tagataaag agttatctc 
aatcagttg tgtgaatta gaaaaaaat aatgaaaat 
tcggtgtat atatctctt ataatcgtg gaaaagttt 
accacttgt tcttgacgg ataaaaaaa aacgctatt 
ataagaatt cgttctgct catgagtgg actacgtcc 
acggattgt ctttttttt ctaattagt cgcagactt 
gagtcagct ccagctgac gctaatatc caaacggta 
ttgtatcat agttttcta aaacaaccc tcttatcat 
aaacaaaac caaattctt ttgtttcga ttttattta 
agagttcct tagttttag tatctctgg taattcgat 
tatattgcg tactgattt tactgtctt tcttttcta 
aaatattat ttatacttt cggaattaa tctttttgg 
acgtttcat aatttagcg taaatcctt ttattttac 
aaacaactt tacctgtgt ttgttttat aacaataac 
ttttaggac ctgttgata tccaatcta ttatccata 
cgtctttac ctaattcgt ctgtccatt ctcccaata 
aacagtctg ttttgatag tatttttac catcgtcta 
tatatacaa cgctatcag tatcataat atacaactg 
catctccca atttatcta acatttcgt acaatctta 
gtcttgcgt ttgcagttg taaaagcag ctataaata 
tattggtat tgaaattat tttcaacat aacaatctt 
ttaacttat aatcaattt gtaacatat tttcattta 
tatcgttta tatgtatat tatcaagtc tgtcatcta 
ataaaattt cataaaatc ttttaacat ctgtaacat 
attctgttg cacc
    • >TC031360 RB
      tcagccccc aagaaacac acgcatttt attgaaaaa 
ggagccgcc gccgggggc gagatttca gttcggcga 
cggcgacgg aggtaggag tggcgtcgc tcttgcgtc 
tgtggagat gtgcgcggc gcagcttca tcaacggtg 
tctctctcc agtttccgc gcgctcttc caaatagca 
ggacgtcga acacaactg gccaaaagc ggcttttcc 
aacttttta gtgacactt tttccctct cggtgcaaa 
aaatgcgag cgtcttgtc ggcgtcgag gcgcttcgt 
gaagtggtg cgtgtgtgt ggtgagctc aagaagcga 
caaaatgtg cgacgtccg acgcaggac ttgggtctt 
gttggggtt gctgctggg attgtttac cggtgggga 
cgctctcaa ggacgtatc agtgaccat tcctcccgc 
cgtcaaaat catggacac cgtcacgct gcagtgccg 
gtacgacct ccaagggga gcccctcta caccgtcaa 
gtggtacaa gggcggcca ggagttctt ccggtacat 
ccccaagga gctgccaag cacgcaggt gttccctct 
gccggggat caacgtcga tctgagcaa gtccagccc 
taacgaggt cgtcctgcg aaatgtgca gccggaagt 
caccggacg atacaaatg cgaggtttc ctcagacgc 
accaaattt ctacacttt catagtctc ggggtacat 
gtacgtgat cggtcttac tgccatcca tatccttag 
agctggtca gttacatga ggatgttac atattttaa 
aatcgcttc gctcatgag ggcttcgcc ggaattaac 
atttttcaa agattttac tctatagga taattatat 
aactactta tatcaaaaa tagataaag agttatctc 
aatcagttg tgtgaatta gaaaaaaat aatgaaaat 
tcggtgtat atatctctt ataatcgtg gaaaagttt 
accacttgt tcttgacgg ataaaaaaa aacgctatt 
ataagaatt cgttctgct catgagtgg actacgtcc 
acggattgt ctttttttt ctaattagt cgcagactt 
gagtcagct ccagctgac gctaatatc caaacggta 
ttgtatcat agttttcta aaacaaccc tcttatcat 
aaacaaaac caaattctt ttgtttcga ttttattta 
agagttcct tagttttag tatctctgg taattcgat 
tatattgcg tactgattt tactgtctt tcttttcta 
aaatattat ttatacttt cggaattaa tctttttgg 
acgtttcat aatttagcg taaatcctt ttattttac 
aaacaactt tacctgtgt ttgttttat aacaataac 
ttttaggac ctgttgata tccaatcta ttatccata 
cgtctttac ctaattcgt ctgtccatt ctcccaata 
aacagtctg ttttgatag tatttttac catcgtcta 
tatatacaa cgctatcag tatcataat atacaactg 
catctccca atttatcta acatttcgt acaatctta 
gtcttgcgt ttgcagttg taaaagcag ctataaata 
tattggtat tgaaattat tttcaacat aacaatctt 
ttaacttat aatcaattt gtaacatat tttcattta 
tatcgttta tatgtatat tatcaagtc tgtcatcta 
ataaaattt cataaaatc ttttaacat ctgtaacat 
attctgttg cacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY