| General information | |||
| TC033514 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1455592..1461616 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033514 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08588 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
tropharium anterior structure irregular - Number of animals showing the phenotype: 1
vitellarium stage sequence irregular - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033514 RA
MAADSDGCD LTTIVTCEG NLSDPDFAN RFKAVVGTL NGILGDVVT VHKLEPWNS VRVTLSIPR EAALRLRKL ASEGSAQLR NLGILSVQV EGDQVISLR IANSVNAEP QEIRLQTTQ DGSGSSQDG PLSRFLAPA TEPEKVQFK SPNVVCPPD SVVPKVPVS AATVVSTTP PTTKSYAGP FPFTSMNQA IHSQANYAT PPPPYPGKH PAVTISSPL LVNLLQNDG ATSPKTTET PVSSAVNRT VNSFVQKPN LPRTIVRQK NTKPQANTS PSALSNTSI RQNSKPNST ITPPPYPNN TATTHLPPP PPYSVAVTR QWDSVVQTS PFLDLTPSL TDDLEHLLP TLERDLARS PPEIPEELT HGDDKRNFL INPLTGELE PQSSGESET EDLGDVFTG LPSPAALSD DDTNSTIRP DTTDQSDSE TRSSHSDSG KHSRIKTKT VRDRGRDSP NLKPTEKIK LRLKLEKSE PINTAYKVD VSFIQQPKK ASTSVVAAS PGEELRVPP LHISLRGRH HAFITNKKK KLTDKAKMR KKIATDSAI IDETDEKKK KFKSNNHDS TDLISKLVI HNNYKDKQK ERRGSDSEL TRANLKFVD SNGIISVEK KRRLSQSEP GLESEPPIL GSTNVGTIA SLPQKSRKD KSKVKEVFK SKDVVRNKS FSKSCLGEK FTKQVALPT GEIDMGAKF KQSLMEGTE KGIPRPPHR TENVHQIGV ENKKIEKAD QIKVEAEPP PLKEKSPEP DKCNTPDRK QADLGDKQA GRSPNSGAQ GEDSGIESM DALSEKSPN QASQSPHAD IPEPIKSKT QVPDMPHML DIEAQLAKM EGLNGVEDM NENQHNTQP KQLGQCCEL TSALQDSLK QGAVSLSSV NVASPHAVK EQLQPAEVT LVPVKTKTK EDDLEPLPV RVTPPLYTY SNPEKGRGS ESPTMSDED SNSCSNNIH KTKSLLEQL LIEIPDNQT PSSPSPATR SVRTRALSK LNSPELNSP VSTTIKPNR VATTPAAKR KRHESDSSN NSIDDTRNK KTRKCSENA VELIKACIG VEATKANNV KKSAKSQDE SSDSDEPLI EKVRKTATA NSVGKKAKS GNGVKGVAV TTRRSVRTT AIPAPNTRS KGDKVGVDS EALRRKTRS AVSEGDSKR KKEVK
- >TC033514 RA
atggcagca gacagtgat ggctgcgat ttaacgaca atagtcaca tgcgaaggc aatctttca gaccctgac ttcgcaaat cgcttcaaa gctgtcgtc ggcacactg aacggtatt ctgggagac gtcgtcaca gtccacaag ctggagccc tggaattcg gtgcgtgta acgctgtct attcctcgg gaagcagcg ttacgtttg aggaagctg gccagcgag ggctcggcc cagctccgc aacctgggg atactcagt gtgcaggtg gaaggcgac caagtcatc tccttgcgc atcgccaac tccgtcaac gcggagccc caggaaatc cgcctccaa accacccaa gatggcagc ggtagcagt caagacggc cccttgtct cgattcctg gcccctgcg acggaaccg gaaaaagtg cagttcaag tcacccaac gtggtgtgc ccccctgac agtgtggtc cctaaagta cccgtcagc gctgcaacg gttgttagc accacgccc ccgaccacc aaaagctac gcgggacct ttccccttc accagcatg aaccaggcg atccatagt caagcgaat tacgccact ccaccgccc ccctatcca ggaaaacac ccagcggtg acgatctcc agccccctt ctggtgaac ctgcttcag aacgacggg gcaacgtca ccgaaaaca accgaaact ccagtgagc agtgcagtg aacaggacg gtgaattcg tttgtgcaa aaaccgaac ttgcctcga actatagtc aggcagaag aacaccaaa cctcaggca aacacgtca ccaagcgcc ttatcaaat acttccatt aggcagaat agtaaacca aacagtaca attacacca ccaccgtac cccaacaac acggcaaca actcatctg cccccaccg cccccctac tcggtggcg gtcactcgc cagtgggac tccgtagta caaacttca cctttcttg gacttgacg ccttcactc accgacgac ttggagcat ctccttcca acactggag cgtgacctt gccaggagc cctcccgaa atcccagag gaattgacc cacggggac gacaaacgc aactttctc atcaacccc ttaaccggc gaattggaa ccgcaatcg agcggcgag agcgaaacc gaagacctg ggggatgtc tttaccgga ctaccatca cccgcagct ctgtcggac gacgacacc aattcaaca atacgccca gacacaacg gaccaaagt gacagtgaa acgcgctcc tcacacagt gacagtggc aaacactcc cgtatcaaa acgaaaacg gtccgtgac cgtggtcgc gactcccca aaccttaaa ccaaccgaa aaaatcaaa ctccgactc aaattggaa aaatccgaa ccgatcaat accgcctac aaagtcgat gttagtttc atccaacag ccgaagaag gcgtccacg tccgtcgtc gccgcgtca ccaggtgaa gaactccgc gttccccct ttacacatt tcgttacgt ggacgccac cacgcattc atcaccaac aagaagaaa aagctcaca gacaaggcg aaaatgcgc aagaaaatc gccaccgac agtgcaata atcgacgaa accgacgag aaaaagaag aaattcaag tcgaataat catgattcg acggacctc atctccaaa ttggtcatt cacaacaac tacaaggac aagcaaaag gaacgacgc ggaagcgat tcggaattg acacgagcc aatttgaaa tttgtcgat agtaatgga attatttcc gttgagaag aagcgtcga ttgagtcag tctgagccg ggtctcgaa tcagaaccg ccgattttg ggttcgacc aatgtcgga acaattgca agtctaccg caaaaatcg cgaaaagac aagagtaaa gtcaaggag gtgttcaag agtaaagat gtagtcagg aataagagt tttagtaag agttgtttg ggtgaaaag tttacgaaa caggtggcg ttgccgacg ggtgaaatt gacatgggg gcgaaattt aagcaaagt ttgatggaa ggcacggaa aagggaatt ccaaggccg ccgcatcgg actgaaaat gtacatcag atcggtgtg gagaataag aaaatcgaa aaggctgat cagataaaa gtggaggcg gagccgcct cccctgaag gagaaatcc cccgagccg gacaagtgc aacactccc gatcgcaaa caggccgat ttgggcgat aaacaggcg ggccgttca ccgaattcc ggtgcccaa ggcgaagac agcggtatc gaaagtatg gacgctcta agcgaaaaa tcaccgaat caggccagc caaagtcca cacgccgac attcccgaa ccaatcaaa tcaaaaaca caagtgcct gacatgcct cacatgctc gacattgaa gcccaattg gccaaaatg gaaggtttg aacggcgtt gaagacatg aacgagaac caacacaac acccaaccg aaacagttg ggtcagtgt tgcgaatta acttcggca ctgcaagat agtctcaaa caaggagct gtcagtctg tcatcggtc aacgtggcg agtccgcac gctgtgaaa gaacaactt caaccggcg gaagtcact ctcgttccg gtcaagacc aagacgaaa gaagacgat ttggagccg cttccggtt cgtgtaacg ccgcctctc tacacttat tcgaatccg gagaaaggc cgaggttcc gaaagtccg acaatgtcg gatgaagac agcaattcg tgttccaac aacatccac aaaacgaaa agtctcctc gaacagctc ctgattgaa atacctgat aatcaaacg ccgagttca ccgagccct gcgacacgt tcggttcgc actagagcc ctctcgaag ttgaacagt cccgaatta aattcgccg gtttcgaca acaattaaa ccaaatcgt gttgcgacg acaccagcc gccaaaagg aaacgacac gaatcggac agttccaac aacagcata gacgatacg aggaataaa aagacaagg aaatgctcg gaaaatgca gttgaactg attaaggca tgtattggt gtcgaggcg accaaagcg aataatgtt aaaaagtcg gcgaaatcg caagacgaa agttcggac agtgacgaa ccgctgatt gagaaagtc aggaaaacg gcaacggcg aatagtgtg gggaagaag gccaaaagt gggaatggg gtcaaaggt gttgcggtg acgacgagg aggtcggtg aggacgaca gcgatcccg gcgccgaat acgagaagt aaaggggat aaagttggg gtggattcg gaagcgctg aggaggaag acaaggagt gctgtgtcg gagggcgac agcaaacgc aagaaagag gtaaagtga
- >TC033514 RA
cgattttga caaggtgcg catctagtg agcgcatct cgaatggcg tcgagttgt tattgttga aagcgttta ataatttct ttaaggatt atgagtgtt tttatggag tttttaaaa gccttcttg caaattagt gatcctaaa gtatactag tacaagtgc agaggagaa ctttccggc tggattggc tttttgagt gtcaaaagt atgtcacag agaatcggg ttgatgtaa aacttcgat aatcggaat gtgactccc accaagggt aagtcggcg gatgagaga tggggcctc tctaacttt gccgatttt agatggcag cagacagtg atggctgcg atttaacga caatagtca catgcgaag gcaatcttt cagaccctg acttcgcaa atcgcttca aagctgtcg tcggcacac tgaacggta ttctgggag acgtcgtca cagtccaca agctggagc cctggaatt cggtgcgtg taacgctgt ctattcctc gggaagcag cgttacgtt tgaggaagc tggccagcg agggctcgg cccagctcc gcaacctgg ggatactca gtgtgcagg tggaaggcg accaagtca tctccttgc gcatcgcca actccgtca acgcggagc cccaggaaa tccgcctcc aaaccaccc aagatggca gcggtagca gtcaagacg gccccttgt ctcgattcc tggcccctg cgacggaac cggaaaaag tgcagttca agtcaccca acgtggtgt gcccccctg acagtgtgg tccctaaag tacccgtca gcgctgcaa cggttgtta gcaccacgc ccccgacca ccaaaagct acgcgggac ctttcccct tcaccagca tgaaccagg cgatccata gtcaagcga attacgcca ctccaccgc ccccctatc caggaaaac acccagcgg tgacgatct ccagccccc ttctggtga acctgcttc agaacgacg gggcaacgt caccgaaaa caaccgaaa ctccagtga gcagtgcag tgaacagga cggtgaatt cgtttgtgc aaaaaccga acttgcctc gaactatag tcaggcaga agaacacca aacctcagg caaacacgt caccaagcg ccttatcaa atacttcca ttaggcaga atagtaaac caaacagta caattacac caccaccgt accccaaca acacggcaa caactcatc tgcccccac cgcccccct actcggtgg cggtcactc gccagtggg actccgtag tacaaactt cacctttct tggacttga cgccttcac tcaccgacg acttggagc atctccttc caacactgg agcgtgacc ttgccagga gccctcccg aaatcccag aggaattga cccacgggg acgacaaac gcaactttc tcatcaacc ccttaaccg gcgaattgg aaccgcaat cgagcggcg agagcgaaa ccgaagacc tgggggatg tctttaccg gactaccat cacccgcag ctctgtcgg acgacgaca ccaattcaa caatacgcc cagacacaa cggaccaaa gtgacagtg aaacgcgct cctcacaca gtgacagtg gcaaacact cccgtatca aaacgaaaa cggtccgtg accgtggtc gcgactccc caaacctta aaccaaccg aaaaaatca aactccgac tcaaattgg aaaaatccg aaccgatca ataccgcct acaaagtcg atgttagtt tcatccaac agccgaaga aggcgtcca cgtccgtcg tcgccgcgt caccaggtg aagaactcc gcgttcccc ctttacaca tttcgttac gtggacgcc accacgcat tcatcacca acaagaaga aaaagctca cagacaagg cgaaaatgc gcaagaaaa tcgccaccg acagtgcaa taatcgacg aaaccgacg agaaaaaga agaaattca agtcgaata atcatgatt cgacggacc tcatctcca aattggtca ttcacaaca actacaagg acaagcaaa aggaacgac gcggaagcg attcggaat tgacacgag ccaatttga aatttgtcg atagtaatg gaattattt ccgttgaga agaagcgtc gattgagtc agtctgagc cgggtctcg aatcagaac cgccgattt tgggttcga ccaatgtcg gaacaattg caagtctac cgcaaaaat cgcgaaaag acaagagta aagtcaagg aggtgttca agagtaaag atgtagtca ggaataaga gttttagta agagttgtt tgggtgaaa agtttacga aacaggtgg cgttgccga cgggtgaaa ttgacatgg gggcgaaat ttaagcaaa gtttgatgg aaggcacgg aaaagggaa ttccaaggc cgccgcatc ggactgaaa atgtacatc agatcggtg tggagaata agaaaatcg aaaaggctg atcagataa aagtggagg cggagccgc ctcccctga aggagaaat cccccgagc cggacaagt gcaacactc ccgatcgca aacaggccg atttgggcg ataaacagg cgggccgtt caccgaatt ccggtgccc aaggcgaag acagcggta tcgaaagta tggacgctc taagcgaaa aatcaccga atcaggcca gccaaagtc cacacgccg acattcccg aaccaatca aatcaaaaa cacaagtgc ctgacatgc ctcacatgc tcgacattg aagcccaat tggccaaaa tggaaggtt tgaacggcg ttgaagaca tgaacgaga accaacaca acacccaac cgaaacagt tgggtcagt gttgcgaat taacttcgg cactgcaag atagtctca aacaaggag ctgtcagtc tgtcatcgg tcaacgtgg cgagtccgc acgctgtga aagaacaac ttcaaccgg cggaagtca ctctcgttc cggtcaaga ccaagacga aagaagacg atttggagc cgcttccgg ttcgtgtaa cgccgcctc tctacactt attcgaatc cggagaaag gccgaggtt ccgaaagtc cgacaatgt cggatgaag acagcaatt cgtgttcca acaacatcc acaaaacga aaagtctcc tcgaacagc tcctgattg aaatacctg ataatcaaa cgccgagtt caccgagcc ctgcgacac gttcggttc gcactagag ccctctcga agttgaaca gtcccgaat taaattcgc cggtttcga caacaatta aaccaaatc gtgttgcga cgacaccag ccgccaaaa ggaaacgac acgaatcgg acagttcca acaacagca tagacgata cgaggaata aaaagacaa ggaaatgct cggaaaatg cagttgaac tgattaagg catgtattg gtgtcgagg cgaccaaag cgaataatg ttaaaaagt cggcgaaat cgcaagacg aaagttcgg acagtgacg aaccgctga ttgagaaag tcaggaaaa cggcaacgg cgaatagtg tggggaaga aggccaaaa gtgggaatg gggtcaaag gtgttgcgg tgacgacga ggaggtcgg tgaggacga cagcgatcc cggcgccga atacgagaa gtaaagggg ataaagttg gggtggatt cggaagcgc tgaggagga agacaagga gtgctgtgt cggagggcg acagcaaac gcaagaaag aggtaaagt gacagtatc agagtggcc gtccatccg acggtctcc agctactag aagcagagc ctctcacta ggcccccat acgtaacag tgcccatac tgcagcccc agccttgaa cacctttaa tttgaaaat taaacataa aaaagttaa acagcagcc acagccatg tcaccgtta ctgtacaga ttgtgatat acccactat aatattgta aatagctag gatcaatta ttgtaaata gaagagaga aaacaagag agaagcagt gtcacaaag tcacacccg tttatataa atgtaatat aatgtggta ttttccata cgagctaac gcatatata atacataat acatactat tctttacta ttgtttgca ttttacgta gaaaaacat caaatatct cacgattgt atgtacatt gtatgatta ttgtaaagt tgttgacat attgtttac ttttagctt agttgtatc tgtttttct ttattatta ttgattttt taactatta tttatacca caaagcctt gtagggtat gtttcttgt ttctttacg taaaataca atgacttgt aatgttctt aattgtaga tttgtggaa aggttgatg attataaac tatgcattg ttaaataga aatatctt
