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Gene information
General information
TC033514 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1455592..1461616
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033514
    iBeetle screen iB_08588

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 2

    tropharium anterior structure irregular - Number of animals showing the phenotype: 1

    vitellarium stage sequence irregular - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033514 RA
      MAADSDGCD LTTIVTCEG NLSDPDFAN RFKAVVGTL 
      NGILGDVVT VHKLEPWNS VRVTLSIPR EAALRLRKL 
      ASEGSAQLR NLGILSVQV EGDQVISLR IANSVNAEP 
      QEIRLQTTQ DGSGSSQDG PLSRFLAPA TEPEKVQFK 
      SPNVVCPPD SVVPKVPVS AATVVSTTP PTTKSYAGP 
      FPFTSMNQA IHSQANYAT PPPPYPGKH PAVTISSPL 
      LVNLLQNDG ATSPKTTET PVSSAVNRT VNSFVQKPN 
      LPRTIVRQK NTKPQANTS PSALSNTSI RQNSKPNST 
      ITPPPYPNN TATTHLPPP PPYSVAVTR QWDSVVQTS 
      PFLDLTPSL TDDLEHLLP TLERDLARS PPEIPEELT 
      HGDDKRNFL INPLTGELE PQSSGESET EDLGDVFTG 
      LPSPAALSD DDTNSTIRP DTTDQSDSE TRSSHSDSG 
      KHSRIKTKT VRDRGRDSP NLKPTEKIK LRLKLEKSE 
      PINTAYKVD VSFIQQPKK ASTSVVAAS PGEELRVPP 
      LHISLRGRH HAFITNKKK KLTDKAKMR KKIATDSAI 
      IDETDEKKK KFKSNNHDS TDLISKLVI HNNYKDKQK 
      ERRGSDSEL TRANLKFVD SNGIISVEK KRRLSQSEP 
      GLESEPPIL GSTNVGTIA SLPQKSRKD KSKVKEVFK 
      SKDVVRNKS FSKSCLGEK FTKQVALPT GEIDMGAKF 
      KQSLMEGTE KGIPRPPHR TENVHQIGV ENKKIEKAD 
      QIKVEAEPP PLKEKSPEP DKCNTPDRK QADLGDKQA 
      GRSPNSGAQ GEDSGIESM DALSEKSPN QASQSPHAD 
      IPEPIKSKT QVPDMPHML DIEAQLAKM EGLNGVEDM 
      NENQHNTQP KQLGQCCEL TSALQDSLK QGAVSLSSV 
      NVASPHAVK EQLQPAEVT LVPVKTKTK EDDLEPLPV 
      RVTPPLYTY SNPEKGRGS ESPTMSDED SNSCSNNIH 
      KTKSLLEQL LIEIPDNQT PSSPSPATR SVRTRALSK 
      LNSPELNSP VSTTIKPNR VATTPAAKR KRHESDSSN 
      NSIDDTRNK KTRKCSENA VELIKACIG VEATKANNV 
      KKSAKSQDE SSDSDEPLI EKVRKTATA NSVGKKAKS 
      GNGVKGVAV TTRRSVRTT AIPAPNTRS KGDKVGVDS 
      EALRRKTRS AVSEGDSKR KKEVK
    CDS sequence
    • >TC033514 RA
      atggcagca gacagtgat ggctgcgat ttaacgaca 
      atagtcaca tgcgaaggc aatctttca gaccctgac 
      ttcgcaaat cgcttcaaa gctgtcgtc ggcacactg 
      aacggtatt ctgggagac gtcgtcaca gtccacaag 
      ctggagccc tggaattcg gtgcgtgta acgctgtct 
      attcctcgg gaagcagcg ttacgtttg aggaagctg 
      gccagcgag ggctcggcc cagctccgc aacctgggg 
      atactcagt gtgcaggtg gaaggcgac caagtcatc 
      tccttgcgc atcgccaac tccgtcaac gcggagccc 
      caggaaatc cgcctccaa accacccaa gatggcagc 
      ggtagcagt caagacggc cccttgtct cgattcctg 
      gcccctgcg acggaaccg gaaaaagtg cagttcaag 
      tcacccaac gtggtgtgc ccccctgac agtgtggtc 
      cctaaagta cccgtcagc gctgcaacg gttgttagc 
      accacgccc ccgaccacc aaaagctac gcgggacct 
      ttccccttc accagcatg aaccaggcg atccatagt 
      caagcgaat tacgccact ccaccgccc ccctatcca 
      ggaaaacac ccagcggtg acgatctcc agccccctt 
      ctggtgaac ctgcttcag aacgacggg gcaacgtca 
      ccgaaaaca accgaaact ccagtgagc agtgcagtg 
      aacaggacg gtgaattcg tttgtgcaa aaaccgaac 
      ttgcctcga actatagtc aggcagaag aacaccaaa 
      cctcaggca aacacgtca ccaagcgcc ttatcaaat 
      acttccatt aggcagaat agtaaacca aacagtaca 
      attacacca ccaccgtac cccaacaac acggcaaca 
      actcatctg cccccaccg cccccctac tcggtggcg 
      gtcactcgc cagtgggac tccgtagta caaacttca 
      cctttcttg gacttgacg ccttcactc accgacgac 
      ttggagcat ctccttcca acactggag cgtgacctt 
      gccaggagc cctcccgaa atcccagag gaattgacc 
      cacggggac gacaaacgc aactttctc atcaacccc 
      ttaaccggc gaattggaa ccgcaatcg agcggcgag 
      agcgaaacc gaagacctg ggggatgtc tttaccgga 
      ctaccatca cccgcagct ctgtcggac gacgacacc 
      aattcaaca atacgccca gacacaacg gaccaaagt 
      gacagtgaa acgcgctcc tcacacagt gacagtggc 
      aaacactcc cgtatcaaa acgaaaacg gtccgtgac 
      cgtggtcgc gactcccca aaccttaaa ccaaccgaa 
      aaaatcaaa ctccgactc aaattggaa aaatccgaa 
      ccgatcaat accgcctac aaagtcgat gttagtttc 
      atccaacag ccgaagaag gcgtccacg tccgtcgtc 
      gccgcgtca ccaggtgaa gaactccgc gttccccct 
      ttacacatt tcgttacgt ggacgccac cacgcattc 
      atcaccaac aagaagaaa aagctcaca gacaaggcg 
      aaaatgcgc aagaaaatc gccaccgac agtgcaata 
      atcgacgaa accgacgag aaaaagaag aaattcaag 
      tcgaataat catgattcg acggacctc atctccaaa 
      ttggtcatt cacaacaac tacaaggac aagcaaaag 
      gaacgacgc ggaagcgat tcggaattg acacgagcc 
      aatttgaaa tttgtcgat agtaatgga attatttcc 
      gttgagaag aagcgtcga ttgagtcag tctgagccg 
      ggtctcgaa tcagaaccg ccgattttg ggttcgacc 
      aatgtcgga acaattgca agtctaccg caaaaatcg 
      cgaaaagac aagagtaaa gtcaaggag gtgttcaag 
      agtaaagat gtagtcagg aataagagt tttagtaag 
      agttgtttg ggtgaaaag tttacgaaa caggtggcg 
      ttgccgacg ggtgaaatt gacatgggg gcgaaattt 
      aagcaaagt ttgatggaa ggcacggaa aagggaatt 
      ccaaggccg ccgcatcgg actgaaaat gtacatcag 
      atcggtgtg gagaataag aaaatcgaa aaggctgat 
      cagataaaa gtggaggcg gagccgcct cccctgaag 
      gagaaatcc cccgagccg gacaagtgc aacactccc 
      gatcgcaaa caggccgat ttgggcgat aaacaggcg 
      ggccgttca ccgaattcc ggtgcccaa ggcgaagac 
      agcggtatc gaaagtatg gacgctcta agcgaaaaa 
      tcaccgaat caggccagc caaagtcca cacgccgac 
      attcccgaa ccaatcaaa tcaaaaaca caagtgcct 
      gacatgcct cacatgctc gacattgaa gcccaattg 
      gccaaaatg gaaggtttg aacggcgtt gaagacatg 
      aacgagaac caacacaac acccaaccg aaacagttg 
      ggtcagtgt tgcgaatta acttcggca ctgcaagat 
      agtctcaaa caaggagct gtcagtctg tcatcggtc 
      aacgtggcg agtccgcac gctgtgaaa gaacaactt 
      caaccggcg gaagtcact ctcgttccg gtcaagacc 
      aagacgaaa gaagacgat ttggagccg cttccggtt 
      cgtgtaacg ccgcctctc tacacttat tcgaatccg 
      gagaaaggc cgaggttcc gaaagtccg acaatgtcg 
      gatgaagac agcaattcg tgttccaac aacatccac 
      aaaacgaaa agtctcctc gaacagctc ctgattgaa 
      atacctgat aatcaaacg ccgagttca ccgagccct 
      gcgacacgt tcggttcgc actagagcc ctctcgaag 
      ttgaacagt cccgaatta aattcgccg gtttcgaca 
      acaattaaa ccaaatcgt gttgcgacg acaccagcc 
      gccaaaagg aaacgacac gaatcggac agttccaac 
      aacagcata gacgatacg aggaataaa aagacaagg 
      aaatgctcg gaaaatgca gttgaactg attaaggca 
      tgtattggt gtcgaggcg accaaagcg aataatgtt 
      aaaaagtcg gcgaaatcg caagacgaa agttcggac 
      agtgacgaa ccgctgatt gagaaagtc aggaaaacg 
      gcaacggcg aatagtgtg gggaagaag gccaaaagt 
      gggaatggg gtcaaaggt gttgcggtg acgacgagg 
      aggtcggtg aggacgaca gcgatcccg gcgccgaat 
      acgagaagt aaaggggat aaagttggg gtggattcg 
      gaagcgctg aggaggaag acaaggagt gctgtgtcg 
      gagggcgac agcaaacgc aagaaagag gtaaagtga
    mRNA sequence
    • >TC033514 RA
      cgattttga caaggtgcg catctagtg agcgcatct 
      cgaatggcg tcgagttgt tattgttga aagcgttta 
      ataatttct ttaaggatt atgagtgtt tttatggag 
      tttttaaaa gccttcttg caaattagt gatcctaaa 
      gtatactag tacaagtgc agaggagaa ctttccggc 
      tggattggc tttttgagt gtcaaaagt atgtcacag 
      agaatcggg ttgatgtaa aacttcgat aatcggaat 
      gtgactccc accaagggt aagtcggcg gatgagaga 
      tggggcctc tctaacttt gccgatttt agatggcag 
      cagacagtg atggctgcg atttaacga caatagtca 
      catgcgaag gcaatcttt cagaccctg acttcgcaa 
      atcgcttca aagctgtcg tcggcacac tgaacggta 
      ttctgggag acgtcgtca cagtccaca agctggagc 
      cctggaatt cggtgcgtg taacgctgt ctattcctc 
      gggaagcag cgttacgtt tgaggaagc tggccagcg 
      agggctcgg cccagctcc gcaacctgg ggatactca 
      gtgtgcagg tggaaggcg accaagtca tctccttgc 
      gcatcgcca actccgtca acgcggagc cccaggaaa 
      tccgcctcc aaaccaccc aagatggca gcggtagca 
      gtcaagacg gccccttgt ctcgattcc tggcccctg 
      cgacggaac cggaaaaag tgcagttca agtcaccca 
      acgtggtgt gcccccctg acagtgtgg tccctaaag 
      tacccgtca gcgctgcaa cggttgtta gcaccacgc 
      ccccgacca ccaaaagct acgcgggac ctttcccct 
      tcaccagca tgaaccagg cgatccata gtcaagcga 
      attacgcca ctccaccgc ccccctatc caggaaaac 
      acccagcgg tgacgatct ccagccccc ttctggtga 
      acctgcttc agaacgacg gggcaacgt caccgaaaa 
      caaccgaaa ctccagtga gcagtgcag tgaacagga 
      cggtgaatt cgtttgtgc aaaaaccga acttgcctc 
      gaactatag tcaggcaga agaacacca aacctcagg 
      caaacacgt caccaagcg ccttatcaa atacttcca 
      ttaggcaga atagtaaac caaacagta caattacac 
      caccaccgt accccaaca acacggcaa caactcatc 
      tgcccccac cgcccccct actcggtgg cggtcactc 
      gccagtggg actccgtag tacaaactt cacctttct 
      tggacttga cgccttcac tcaccgacg acttggagc 
      atctccttc caacactgg agcgtgacc ttgccagga 
      gccctcccg aaatcccag aggaattga cccacgggg 
      acgacaaac gcaactttc tcatcaacc ccttaaccg 
      gcgaattgg aaccgcaat cgagcggcg agagcgaaa 
      ccgaagacc tgggggatg tctttaccg gactaccat 
      cacccgcag ctctgtcgg acgacgaca ccaattcaa 
      caatacgcc cagacacaa cggaccaaa gtgacagtg 
      aaacgcgct cctcacaca gtgacagtg gcaaacact 
      cccgtatca aaacgaaaa cggtccgtg accgtggtc 
      gcgactccc caaacctta aaccaaccg aaaaaatca 
      aactccgac tcaaattgg aaaaatccg aaccgatca 
      ataccgcct acaaagtcg atgttagtt tcatccaac 
      agccgaaga aggcgtcca cgtccgtcg tcgccgcgt 
      caccaggtg aagaactcc gcgttcccc ctttacaca 
      tttcgttac gtggacgcc accacgcat tcatcacca 
      acaagaaga aaaagctca cagacaagg cgaaaatgc 
      gcaagaaaa tcgccaccg acagtgcaa taatcgacg 
      aaaccgacg agaaaaaga agaaattca agtcgaata 
      atcatgatt cgacggacc tcatctcca aattggtca 
      ttcacaaca actacaagg acaagcaaa aggaacgac 
      gcggaagcg attcggaat tgacacgag ccaatttga 
      aatttgtcg atagtaatg gaattattt ccgttgaga 
      agaagcgtc gattgagtc agtctgagc cgggtctcg 
      aatcagaac cgccgattt tgggttcga ccaatgtcg 
      gaacaattg caagtctac cgcaaaaat cgcgaaaag 
      acaagagta aagtcaagg aggtgttca agagtaaag 
      atgtagtca ggaataaga gttttagta agagttgtt 
      tgggtgaaa agtttacga aacaggtgg cgttgccga 
      cgggtgaaa ttgacatgg gggcgaaat ttaagcaaa 
      gtttgatgg aaggcacgg aaaagggaa ttccaaggc 
      cgccgcatc ggactgaaa atgtacatc agatcggtg 
      tggagaata agaaaatcg aaaaggctg atcagataa 
      aagtggagg cggagccgc ctcccctga aggagaaat 
      cccccgagc cggacaagt gcaacactc ccgatcgca 
      aacaggccg atttgggcg ataaacagg cgggccgtt 
      caccgaatt ccggtgccc aaggcgaag acagcggta 
      tcgaaagta tggacgctc taagcgaaa aatcaccga 
      atcaggcca gccaaagtc cacacgccg acattcccg 
      aaccaatca aatcaaaaa cacaagtgc ctgacatgc 
      ctcacatgc tcgacattg aagcccaat tggccaaaa 
      tggaaggtt tgaacggcg ttgaagaca tgaacgaga 
      accaacaca acacccaac cgaaacagt tgggtcagt 
      gttgcgaat taacttcgg cactgcaag atagtctca 
      aacaaggag ctgtcagtc tgtcatcgg tcaacgtgg 
      cgagtccgc acgctgtga aagaacaac ttcaaccgg 
      cggaagtca ctctcgttc cggtcaaga ccaagacga 
      aagaagacg atttggagc cgcttccgg ttcgtgtaa 
      cgccgcctc tctacactt attcgaatc cggagaaag 
      gccgaggtt ccgaaagtc cgacaatgt cggatgaag 
      acagcaatt cgtgttcca acaacatcc acaaaacga 
      aaagtctcc tcgaacagc tcctgattg aaatacctg 
      ataatcaaa cgccgagtt caccgagcc ctgcgacac 
      gttcggttc gcactagag ccctctcga agttgaaca 
      gtcccgaat taaattcgc cggtttcga caacaatta 
      aaccaaatc gtgttgcga cgacaccag ccgccaaaa 
      ggaaacgac acgaatcgg acagttcca acaacagca 
      tagacgata cgaggaata aaaagacaa ggaaatgct 
      cggaaaatg cagttgaac tgattaagg catgtattg 
      gtgtcgagg cgaccaaag cgaataatg ttaaaaagt 
      cggcgaaat cgcaagacg aaagttcgg acagtgacg 
      aaccgctga ttgagaaag tcaggaaaa cggcaacgg 
      cgaatagtg tggggaaga aggccaaaa gtgggaatg 
      gggtcaaag gtgttgcgg tgacgacga ggaggtcgg 
      tgaggacga cagcgatcc cggcgccga atacgagaa 
      gtaaagggg ataaagttg gggtggatt cggaagcgc 
      tgaggagga agacaagga gtgctgtgt cggagggcg 
      acagcaaac gcaagaaag aggtaaagt gacagtatc 
      agagtggcc gtccatccg acggtctcc agctactag 
      aagcagagc ctctcacta ggcccccat acgtaacag 
      tgcccatac tgcagcccc agccttgaa cacctttaa 
      tttgaaaat taaacataa aaaagttaa acagcagcc 
      acagccatg tcaccgtta ctgtacaga ttgtgatat 
      acccactat aatattgta aatagctag gatcaatta 
      ttgtaaata gaagagaga aaacaagag agaagcagt 
      gtcacaaag tcacacccg tttatataa atgtaatat 
      aatgtggta ttttccata cgagctaac gcatatata 
      atacataat acatactat tctttacta ttgtttgca 
      ttttacgta gaaaaacat caaatatct cacgattgt 
      atgtacatt gtatgatta ttgtaaagt tgttgacat 
      attgtttac ttttagctt agttgtatc tgtttttct 
      ttattatta ttgattttt taactatta tttatacca 
      caaagcctt gtagggtat gtttcttgt ttctttacg 
      taaaataca atgacttgt aatgttctt aattgtaga 
      tttgtggaa aggttgatg attataaac tatgcattg 
      ttaaataga aatatctt
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