Status
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Gene information
General information
TC009420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1469010..1485396
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009420
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08587

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands complete content not present - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands content split - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection



    abdominal odoriferous glands colour irregular - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection



    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: there seems to be an A-P-polarity present
    larva potentially circumference decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva segment potentially number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009420 RA
      MEKQMSEEL LVPLSSDEC GHSGGDPDS DEDVITNYH 
      ISSLDMLPR PGPLPTISV TPHSPASKI YPVLEDSLQ 
      QVRELHESV QHMRNVTIP DTYIPNSKD LAIALNPIL 
      SQMARLSSS CPTLNEANQ DMDLLGGSL GSSPVHQNR 
      KGSTQDWLC QPETQRRRS WSALEDLTI KDKAKHTRQ 
      RSISLSSME SEADESSFI DNVDGSTTK LLGPDAPAV 
      LRHRNARGS GGATSTHSL NEADLQNDF NKIKAKREA 
      EQRLLPQRL PLQKSISTP SIIAVRDLA PEPILAGAQ 
      PTLPMGRPS GTESETEEE GSRSGQARY SHSTHLPYV 
      SFEGHSEKR RKRGSLFFR KKKDKNKKV AHQWVSACY 
      GSSQICDVC AKHLSNKPA LYCECCGTT VHQNTCKDN 
      IAECNKPKS TKNSNKMSG FGVPNSKNS FSKRGSASM 
      PGNVNSTSN SQILCDDKD SDLHGHYDG TSFPDDVPL 
      VPLEFLSDS PITAAELCA DLSLGLHEN EPDSWTPHV 
      GKEIARKLK EKEVKRQEH IYEFILTEK HHCMTLLVM 
      QKVFVEGLQ KYFQLGANV ERMFPRLAD LTELHLGLL 
      SRLRQRQRE GPVVSTIAD ILLEQFSNT NASKLKSAY 
      GEFCSRHRD AVELYKYYL QNDARFGQF VRHCQANPL 
      LKKKGIPEC ILFITQRLT KYPLLIEPL IKTSKENKK 
      EQDILHKAL ALIKEILVE VDAQVAEKE KEDRKLEIY 
      NRIDAKSYT VHRDHKFKK SDILQGNRS LKFEGVAML 
      MQGRGKMQI VLVIVLSDV LFFLQENSH KYSFFTPDN 
      KAGVVSLQK LLVREKAGQ DSRGIYLIS SNPADPEMF 
      ELKVHKPKD KQIWIHAIR EAVQSCPED DEDSSVLSA 
      EERQSLLNA KQIRHFVGL LRQKDIEQA LLLEEKMSL 
      QLKLLSAAG IDPPSPSSY RQLVSENVD TGQMWKEVL 
      TAVQEVNRL VSSLYTTGT NLSRSVSSA GEHQSEAYV 
      SPILPKRAE TFGGFDNPN QPPLKVLGK KNHSSGGTP 
      VATPEVETV KPGDSKLFE KLPYRNYVI PTTIGSSNN 
      LNMENSQQL TAPPDAPAL LSLGREQQY TAVKLSHYV 
      YTLLCIISQ LMTTNESYQ AQLNALKVG GEGARQYRH 
      NQQLEELRN LQDKLSGEK AAWIATRDQ EAKELEEKK 
      AELLKLQEQ IRVEQEDIK EQREQLFRK MEKLTNQGL 
      IISPNVGAL SLTGQLDDC KDSSEDSPQ SDSSTSIAS 
      SASGQNSQS TMEKKKDTN KWSKNSLTK SQLPMNLIS 
      TTNQQKVQQ NVQVKQQIP LKLASRLSS SGPLAGPTG 
      TPQILPLKL SQDEKVRRT STSGYQRLS PPSGETTPT 
      TSHSHTRTG SSPASMQVS PPGPAPAQK GARQNFPKI 
      GDEEVIYF
    CDS sequence
    • >TC009420 RA
      atggagaaa caaatgtcc gaagaactt ctggtaccc 
      ctttcttca gacgaatgt ggccacagt ggcggcgat 
      ccagatagt gacgaagac gtcatcaca aactaccac 
      atttcaagc ctggatatg cttcctcgg ccaggccct 
      ttacccaca atcagtgtt acacctcat tcgcctgcc 
      agtaaaatc tatccagtt ctcgaagac agccttcag 
      caagttcgc gaattgcat gagagtgtc cagcacatg 
      cgaaatgtc actataccg gatacgtat ataccgaat 
      tcgaaagac cttgctatt gcacttaat cccatcctg 
      tcccaaatg gcacggctt agctcaagc tgtcccaca 
      cttaacgaa gcaaaccaa gacatggat ttgctgggc 
      ggttccctg ggatcgtca ccagtgcac cagaaccgc 
      aaaggcagc actcaggac tggctctgc cagcctgaa 
      acccagcga aggcgctcc tggtcagcg ctggaagac 
      ctgacaatc aaggacaaa gccaaacac acgcgccag 
      cgaagcatc tcgctgagt agtatggag tcggaagcg 
      gacgagtcc tcatttatc gataatgta gatggtagc 
      actactaag ttactaggt cctgatgcc cctgcggtt 
      ttaaggcac cggaacgcg aggggctca gggggcgcc 
      actagcacg cattcgttg aacgaggct gatttacaa 
      aatgatttt aataaaatt aaggcgaaa agggaggcg 
      gaacagaga ctgctgccg cagaggttg cccttacag 
      aaatctatc tcaacacct tccattatt gcggttcgg 
      gatctggct ccggaaccg attctggca ggcgctcaa 
      cccactcta cccatgggc cgtcccagt ggaaccgaa 
      agcgaaact gaagaagag ggcagccgt tctggtcaa 
      gcccgttac tcgcattct acacatttg ccatacgtt 
      tcgttcgaa ggccattcg gagaaacgg aggaaacgg 
      ggcagcttg tttttcaga aagaaaaag gataaaaat 
      aagaaagtt gcgcatcag tgggtatcg gcctgctac 
      ggatcatcg caaatttgt gatgtgtgt gccaaacat 
      ttgagcaat aaaccggcg ctttactgt gaatgctgt 
      ggaaccaca gtgcaccaa aacacttgt aaagacaac 
      atagctgag tgtaacaaa cccaaaagc acaaagaat 
      agtaacaaa atgtccggt tttggggtt ccaaattct 
      aaaaactcg ttttcgaag cgtggctca gcatcaatg 
      cctggcaat gtaaatagt acttcaaac agccaaatt 
      ttatgtgat gataaggat agcgatttg cacggacat 
      tacgacgga acgagtttt ccggacgat gttccgctg 
      gttcctctc gaatttctc tccgattct cccataact 
      gcagcggaa ttgtgcgcg gatttgtct ctaggtttg 
      cacgagaac gagccggac tcgtggacg ccgcatgtg 
      gggaaggaa atcgcccgg aaattgaag gaaaaagaa 
      gtgaagagg caggaacat atctatgaa ttcatcctc 
      actgagaag catcactgt atgacactg ctcgttatg 
      caaaaagtt tttgttgaa ggtttgcag aaatatttt 
      caattgggg gctaacgtg gaaaggatg tttccccga 
      ttggctgat ttaacggag ctccacttg gggctgctt 
      tcgcggctg agacagcga caaagggaa ggtccggta 
      gtttcgacg attgcggat atcttgctg gagcagttt 
      tccaacact aatgcctcc aaacttaaa tccgcctac 
      ggcgagttt tgcagcaga caccgcgac gccgtcgaa 
      ctctacaaa tattatctc caaaacgac gcccgtttc 
      ggccaattc gtgcgccac tgtcaagcg aaccccctt 
      ttgaagaaa aagggcatt cccgagtgc attttattc 
      ataacccaa cgcctaacc aaatacccg ctcctgatc 
      gaacccctg atcaaaact agcaaagaa aacaagaaa 
      gaacaagac atcctccac aaagccctc gctctgatc 
      aaagagatc ctggtcgaa gtggacgcc caagtggcc 
      gaaaaggag aaagaagac cgcaaattg gagatttac 
      aaccgaatt gacgccaaa tcttacaca gtccaccgc 
      gaccacaaa ttcaaaaaa tcggacatt ttgcaagga 
      aatcgctca ctgaaattc gagggcgta gccatgcta 
      atgcaaggc cggggaaaa atgcaaata gttctagta 
      attgtcctt tcggacgtg ctatttttc ctgcaagaa 
      aactcgcac aaatactcg tttttcaca cccgataac 
      aaagctgga gtggtgtcg ctgcagaag ctgctagtg 
      cgggaaaaa gccggacag gactctcgg gggatttac 
      ctgatttcg tcgaatccg gccgatccg gaaatgttc 
      gagctgaaa gtgcacaag cccaaggac aagcagatt 
      tggatccat gccatacgg gaggcggtg cagtcgtgt 
      ccagaggat gacgaagac agcagtgtc ctaagtgcc 
      gaggagaga cagagtctt ttgaacgca aaacaaatt 
      cggcatttc gtcggtctt ttgcgccaa aaagacata 
      gaacaggcg ttacttctg gaggaaaaa atgtctctg 
      caactcaaa ctgttgagt gctgctgga atcgaccca 
      ccttcgcct tcgtcgtac cgacaactt gtcagcgaa 
      aatgtggac acgggacag atgtggaag gaagtgtta 
      acagcagtc caggaggtc aaccgtttg gtgagctcg 
      ctctacacc actggcacc aacctatcg cgcagtgtg 
      agttcggcg ggcgagcac cagagcgag gcttacgtc 
      tccccaatt ctaccaaaa cgtgccgaa acgttcggt 
      ggcttcgac aatcccaac caaccaccg ttgaaagtt 
      ttaggaaag aaaaaccac tcttcgggt ggcacccct 
      gtggcaaca cccgaagtg gaaaccgtc aaaccaggc 
      gattcgaaa ctattcgaa aagcttccg taccggaat 
      tacgtcatt ccgacgacg attggaagc tcgaataac 
      ttgaatatg gaaaattcg cagcagcta acggcgcca 
      cctgacgcc ccagccttg ctttcgctc ggcagggaa 
      cagcaatat acggccgtt aagttatct cattatgtt 
      tatactctt ctttgtatt atttcgcag ttgatgact 
      acgaatgaa agttatcaa gctcaatta aatgcctta 
      aaagtcgga ggggaggga gcaagacaa tacagacac 
      aatcagcag ttagaagaa ttaagaaat ttgcaagac 
      aagttgagt ggggaaaag gcggcgtgg atcgccacc 
      cgggatcaa gaggccaag gaactggag gagaaaaaa 
      gccgaactt cttaaatta caagaacaa attcgcgtc 
      gaacaagaa gacatcaaa gaacagcgc gagcaattg 
      tttagaaaa atggagaaa ttaacaaac caaggtttg 
      ataatctcc ccaaacgtt ggcgctttg tccttgacc 
      ggccaactt gacgactgt aaagacagt tcggaggac 
      agcccccag tcagacagt tccacttcc atagcgtcg 
      tcagccagc ggtcaaaac tcacaatca acaatggag 
      aagaaaaaa gacactaat aaatggagc aagaactct 
      ctaacgaaa tcgcagttg cccatgaat ctgatcagt 
      accaccaac cagcaaaag gtgcaacag aacgtccaa 
      gtcaagcag caaatcccc ctgaaactg gcgtccaga 
      ctgagctcg agcgggcct ctggcgggc cccacgggg 
      accccccag atcctgccc ctcaagctg agccaagac 
      gaaaaggtc aggcggacg agcacctcg ggctaccag 
      cggctgagt cccccgtcg ggcgaaacc accccgaca 
      acttcacat tcgcacacc aggaccggc tctagcccg 
      gccagcatg caggtgtcc cctccgggg cccgcgccc 
      gcgcaaaag ggcgcccgc caaaatttc cccaaaatc 
      ggcgacgaa gaggtcatt tatttctga
    mRNA sequence
    • >TC009420 RA
      tggtgggag tttcgtgag cctacttta tgaaatcaa 
      ttgtgcaat attcaggca aaatgttcg caaaacact 
      gagataatg tagacttat tgtagaaaa aactactta 
      aaatgtatc aaaagtagt gtcgttagt gttttattt 
      tgaaattta caagatcaa ttgtattac aagaaatct 
      gtgagtact ttttgtgta tgtattgtg tgtaaagtg 
      tgacttttt ctttgctgt ttgtaatag aattactgt 
      tatactgtg cgaaattgg tgtacagtt aaaaaaaaa 
      acttcggta caattttga agaagcatc aagttgcca 
      cctgtctgt aaccatgga gaaacaaat gtccgaaga 
      acttctggt acccctttc ttcagacga atgtggcca 
      cagtggcgg cgatccaga tagtgacga agacgtcat 
      cacaaacta ccacatttc aagcctgga tatgcttcc 
      tcggccagg ccctttacc cacaatcag tgttacacc 
      tcattcgcc tgccagtaa aatctatcc agttctcga 
      agacagcct tcagcaagt tcgcgaatt gcatgagag 
      tgtccagca catgcgaaa tgtcactat accggatac 
      gtatatacc gaattcgaa agaccttgc tattgcact 
      taatcccat cctgtccca aatggcacg gcttagctc 
      aagctgtcc cacacttaa cgaagcaaa ccaagacat 
      ggatttgct gggcggttc cctgggatc gtcaccagt 
      gcaccagaa ccgcaaagg cagcactca ggactggct 
      ctgccagcc tgaaaccca gcgaaggcg ctcctggtc 
      agcgctgga agacctgac aatcaagga caaagccaa 
      acacacgcg ccagcgaag catctcgct gagtagtat 
      ggagtcgga agcggacga gtcctcatt tatcgataa 
      tgtagatgg tagcactac taagttact aggtcctga 
      tgcccctgc ggttttaag gcaccggaa cgcgagggg 
      ctcaggggg cgccactag cacgcattc gttgaacga 
      ggctgattt acaaaatga ttttaataa aattaaggc 
      gaaaaggga ggcggaaca gagactgct gccgcagag 
      gttgccctt acagaaatc tatctcaac accttccat 
      tattgcggt tcgggatct ggctccgga accgattct 
      ggcaggcgc tcaacccac tctacccat gggccgtcc 
      cagtggaac cgaaagcga aactgaaga agagggcag 
      ccgttctgg tcaagcccg ttactcgca ttctacaca 
      tttgccata cgtttcgtt cgaaggcca ttcggagaa 
      acggaggaa acggggcag cttgttttt cagaaagaa 
      aaaggataa aaataagaa agttgcgca tcagtgggt 
      atcggcctg ctacggatc atcgcaaat ttgtgatgt 
      gtgtgccaa acatttgag caataaacc ggcgcttta 
      ctgtgaatg ctgtggaac cacagtgca ccaaaacac 
      ttgtaaaga caacatagc tgagtgtaa caaacccaa 
      aagcacaaa gaatagtaa caaaatgtc cggttttgg 
      ggttccaaa ttctaaaaa ctcgttttc gaagcgtgg 
      ctcagcatc aatgcctgg caatgtaaa tagtacttc 
      aaacagcca aattttatg tgatgataa ggatagcga 
      tttgcacgg acattacga cggaacgag ttttccgga 
      cgatgttcc gctggttcc tctcgaatt tctctccga 
      ttctcccat aactgcagc ggaattgtg cgcggattt 
      gtctctagg tttgcacga gaacgagcc ggactcgtg 
      gacgccgca tgtggggaa ggaaatcgc ccggaaatt 
      gaaggaaaa agaagtgaa gaggcagga acatatcta 
      tgaattcat cctcactga gaagcatca ctgtatgac 
      actgctcgt tatgcaaaa agtttttgt tgaaggttt 
      gcagaaata ttttcaatt gggggctaa cgtggaaag 
      gatgtttcc ccgattggc tgatttaac ggagctcca 
      cttggggct gctttcgcg gctgagaca gcgacaaag 
      ggaaggtcc ggtagtttc gacgattgc ggatatctt 
      gctggagca gttttccaa cactaatgc ctccaaact 
      taaatccgc ctacggcga gttttgcag cagacaccg 
      cgacgccgt cgaactcta caaatatta tctccaaaa 
      cgacgcccg tttcggcca attcgtgcg ccactgtca 
      agcgaaccc ccttttgaa gaaaaaggg cattcccga 
      gtgcatttt attcataac ccaacgcct aaccaaata 
      cccgctcct gatcgaacc cctgatcaa aactagcaa 
      agaaaacaa gaaagaaca agacatcct ccacaaagc 
      cctcgctct gatcaaaga gatcctggt cgaagtgga 
      cgcccaagt ggccgaaaa ggagaaaga agaccgcaa 
      attggagat ttacaaccg aattgacgc caaatctta 
      cacagtcca ccgcgacca caaattcaa aaaatcgga 
      cattttgca aggaaatcg ctcactgaa attcgaggg 
      cgtagccat gctaatgca aggccgggg aaaaatgca 
      aatagttct agtaattgt cctttcgga cgtgctatt 
      tttcctgca agaaaactc gcacaaata ctcgttttt 
      cacacccga taacaaagc tggagtggt gtcgctgca 
      gaagctgct agtgcggga aaaagccgg acaggactc 
      tcgggggat ttacctgat ttcgtcgaa tccggccga 
      tccggaaat gttcgagct gaaagtgca caagcccaa 
      ggacaagca gatttggat ccatgccat acgggaggc 
      ggtgcagtc gtgtccaga ggatgacga agacagcag 
      tgtcctaag tgccgagga gagacagag tcttttgaa 
      cgcaaaaca aattcggca tttcgtcgg tcttttgcg 
      ccaaaaaga catagaaca ggcgttact tctggagga 
      aaaaatgtc tctgcaact caaactgtt gagtgctgc 
      tggaatcga cccaccttc gccttcgtc gtaccgaca 
      acttgtcag cgaaaatgt ggacacggg acagatgtg 
      gaaggaagt gttaacagc agtccagga ggtcaaccg 
      tttggtgag ctcgctcta caccactgg caccaacct 
      atcgcgcag tgtgagttc ggcgggcga gcaccagag 
      cgaggctta cgtctcccc aattctacc aaaacgtgc 
      cgaaacgtt cggtggctt cgacaatcc caaccaacc 
      accgttgaa agttttagg aaagaaaaa ccactcttc 
      gggtggcac ccctgtggc aacacccga agtggaaac 
      cgtcaaacc aggcgattc gaaactatt cgaaaagct 
      tccgtaccg gaattacgt cattccgac gacgattgg 
      aagctcgaa taacttgaa tatggaaaa ttcgcagca 
      gctaacggc gccacctga cgccccagc cttgctttc 
      gctcggcag ggaacagca atatacggc cgttaagtt 
      atctcatta tgtttatac tcttctttg tattatttc 
      gcagttgat gactacgaa tgaaagtta tcaagctca 
      attaaatgc cttaaaagt cggagggga gggagcaag 
      acaatacag acacaatca gcagttaga agaattaag 
      aaatttgca agacaagtt gagtgggga aaaggcggc 
      gtggatcgc cacccggga tcaagaggc caaggaact 
      ggaggagaa aaaagccga acttcttaa attacaaga 
      acaaattcg cgtcgaaca agaagacat caaagaaca 
      gcgcgagca attgtttag aaaaatgga gaaattaac 
      aaaccaagg tttgataat ctccccaaa cgttggcgc 
      tttgtcctt gaccggcca acttgacga ctgtaaaga 
      cagttcgga ggacagccc ccagtcaga cagttccac 
      ttccatagc gtcgtcagc cagcggtca aaactcaca 
      atcaacaat ggagaagaa aaaagacac taataaatg 
      gagcaagaa ctctctaac gaaatcgca gttgcccat 
      gaatctgat cagtaccac caaccagca aaaggtgca 
      acagaacgt ccaagtcaa gcagcaaat ccccctgaa 
      actggcgtc cagactgag ctcgagcgg gcctctggc 
      gggccccac ggggacccc ccagatcct gcccctcaa 
      gctgagcca agacgaaaa ggtcaggcg gacgagcac 
      ctcgggcta ccagcggct gagtccccc gtcgggcga 
      aaccacccc gacaacttc acattcgca caccaggac 
      cggctctag cccggccag catgcaggt gtcccctcc 
      ggggcccgc gcccgcgca aaagggcgc ccgccaaaa 
      tttccccaa aatcggcga cgaagaggt catttattt 
      ctgacggtt ttcgacgaa aatgttcaa ttccgtttc 
      tttgtttgt acgaaggtg taaaagaga taacacgaa 
      tttttatta atgttgacg caatctttc ggtcgataa 
      aacagtttt cactagggg aagacgagt cgctgccga 
      tatcaagtt ggcacaaat tgacttgtg atactagtt 
      tgacagtca aggttgcca aattaaatt gaaaattaa 
      gcagcttga ctacaggaa tagtcaatg cagtgttgg 
      caacacatt ttttactaa aaaaaaata acggagcgt 
      taggcggcg gccgtttcc acccgactt tgggagtac 
      tattagttg tttaatgtg acacttttc gatgtgttc 
      ttttttact ttctcgtgt cttcctaac attataatc 
      agtattgac taatgtact caaaacttg tgacaatac 
      aaagttcta tcataaaat ctactttaa taattcagt 
      agtgcagtt tacaacaat agtgttttc aataatatg 
      tttagtaaa atatacaag aagcgaacg ttcatcacc 
      cgatggaca cacgtttac ttgcttgaa cttgcactg 
      ctgtcaagc tctttaaat tcggactta ttttttacc 
      aaaaaaaga caagcaatt caagtatta tttcgtgag 
      cttggttgt gcggtgcta attcaaaca agtaaacaa 
      agttgttaa taattgtta atagttgtt tatttattt 
      ataaataat tctctgcaa aattggcac cagtacacg 
      actgcttgt acgactgcg attttgcaa agaacgcaa 
      aaggcctac tttttgtct cataaagtg atcgtgtgt 
      aattggaga aatattttt ataagacat atttatcta 
      tctttcaag cagaaactt acgtatgga aggaaactc 
      ggcaattta aagtatttt tattatgta catagcgta 
      acgtgtaag ggttatatc cactcgaaa aataacact 
      tctttatat aattatttt tacttgtta taaagtaag 
      acttgtaaa cagtattaa ttttcgaag tagactgtt 
      taatgttac aatatcctc tattgaaga ttacaccac 
      actgtgtta ataaaaatg tattcgcaa c
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