Status
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Gene information
General information
TC014868 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9417637..9432172
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014868
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    vitellarium size decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 3
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3

    previtellogenic egg chamber potentially position irregular - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014868 RA
      MNNKRKNRG GPLRTQRAR SVIAEGVYN NAHFMHSAT 
      LQIGNVVRI KTASGVVWE GVFKTFSSN FDVVLEVAA 
      KVDNPNSPT QTLLADSFV DKLIFKSGD ISTMEARDV 
      DLEYPTKDT FQTDTAISA RLNGNSNKE EKELEPWEP 
      SSGINGGDN IPLELENAN GWDANDMFR KNEQEYGVT 
      STFDQSLKG YTVPLRSAD TADYREAEA KAAQIANEI 
      ENQPQYKAR LELENGDEE AAFAAVVRP NQEPNDKYI 
      PPAKRKNQN SGKLVRSTP PPSSTQGPS PKGNAPPPM 
      PYPNNVHAM HMPPPMHQM PNHGRPSHT PPHGYNQPM 
      RPPLNQNRA QMNGEAKPR GPRGQVPME QMAAAQQQV 
      KQPPPPQPP QPQQPPPQQ PTPPQQSIS PQQQQQQQQ 
      QQDPVEKVT NTFKKSTLN PNAKEFVLN PAAKPFQPR 
      SPSTPSASR PHTPQTPSH SPYIPATVS GPVGQPHMP 
      VMMPVGYMM TSQPQYQPP PQGSRVRKI PMSQMRTDV 
      TSQMQVAAA TGQPLLAPP IQQFVYPPG MNPQGYHQM 
      HTLRMYETP PQIQYLPPN PSNTPSPAQ PPQYTPGQG 
      PQPPPQQYQ APPPQSHPQ FQPLMCSIL HTQPHMMQN 
      MQYLQQAPP PQHPIPVML PPQHPQAQQ HGPAP
    CDS sequence
    • >TC014868 RA
      atgaacaac aagaggaaa aacagggga gggccgctg 
      aggacccag agggctaga tctgtaata gcagaaggt 
      gtttacaat aatgcacat tttatgcat tctgcgact 
      ctacaaatc ggaaatgtt gttcgtata aaaacggct 
      tccggtgta gtatgggaa ggcgtgttc aagacattt 
      tcgagcaac ttcgatgtc gtcctcgag gtggctgca 
      aaagtcgac aatccaaac agcccaact caaacccta 
      ttggcggat agtttcgtc gataagtta atttttaaa 
      tcgggcgac atatcaacg atggaagca cgcgatgtt 
      gacttggag taccccaca aaggacacg tttcaaaca 
      gacacggct atttcagcc cgtcttaat ggcaattcg 
      aataaggaa gagaaagaa ctcgaaccg tgggaaccc 
      tcttcgggg atcaacggc ggcgacaac atcccctta 
      gagctggag aacgcaaac ggttgggac gccaatgat 
      atgtttcgg aaaaatgag caagaatac ggcgttact 
      agtactttt gaccaatcg ttgaagggc tacactgtg 
      ccgttacgg agtgccgat acggccgat tatcgcgag 
      gccgaagca aaagcggca caaatcgcc aacgaaatc 
      gagaatcaa ccgcagtat aaggcacgc cttgagctc 
      gaaaacggc gatgaagag gcggcgttt gctgccgtt 
      gttcggccg aatcaagaa cctaacgat aagtatatt 
      ccgccggcg aaacgaaag aatcaaaat tcgggaaaa 
      ttggtgcga agtacgcca ccaccttcc tccacacaa 
      ggcccctcc cccaaaggc aacgctccg cctccgatg 
      ccctaccca aacaacgtc cacgcaatg cacatgccc 
      ccgcccatg catcaaatg cccaatcat ggacggccg 
      tcgcatacg cccccgcat gggtacaac caacccatg 
      cggccgccg ttgaaccag aatagggca cagatgaat 
      ggcgaggcg aagccgagg ggtcctcga ggacaagtg 
      cctatggaa cagatggcg gcggcgcag caacaagtt 
      aaacaaccg ccaccacca cagccacca caaccacaa 
      cagccgccg ccacaacaa ccgacgccg cctcaacag 
      agtatatcg ccacaacag caacagcag caacaacaa 
      cagcaagat ccggtggag aaggtgacg aatacgttc 
      aagaagagt acattgaat ccgaatgcg aaggagttt 
      gtgctcaat ccggcggct aaaccgttc cagccacgt 
      tctccaagc acgccgtca gcgagccgc ccccacacc 
      ccccaaacc ccctctcac agcccatac atccctgca 
      acagtgagc ggtcccgta ggccaaccc cacatgcct 
      gtcatgatg ccggttggc tacatgatg acaagtcaa 
      ccccaatac cagcccccg ccacaaggt agccgcgtc 
      cgtaaaatt ccgatgagc caaatgcgt acggacgtc 
      accagtcaa atgcaagtc gctgcggca acaggtcaa 
      ccactccta gctccgccc attcaacaa ttcgtctat 
      ccaccgggc atgaatccg caaggatat catcaaatg 
      cacaccctt cggatgtac gaaaccccg ccccaaatc 
      caatacctc ccacccaat ccatcgaat acgccctcg 
      ccggcccaa ccgccccaa tacacaccg ggccagggc 
      ccccagccg ccgccccaa cagtatcaa gccccgcct 
      ccgcaaagt catccgcag tttcagcct ctcatgtgt 
      tccatactt catacccag ccgcacatg atgcaaaat 
      atgcagtac ctgcagcaa gccccgcct ccgcaacat 
      cccattccg gttatgctc cccccacaa cacccacaa 
      gcgcaacag catgggccg gccccttag
    mRNA sequence
    • >TC014868 RA
      ctagaggaa agaagcaat gaccgaagt gtgttgtgc 
      gcatgcgca gtttgtcca tgtcactct gacagacaa 
      cactttgtg caaatgctg tgttataat tgtttgtta 
      tcacaggaa tcggttttt taaagcacc catccaagc 
      accaggtct cgaacgaag tgtgtcgag gaggacgcc 
      accgcctac ggtaccgtc tgccacgaa tgtgaagag 
      gtgtctttg gagctgcct gctttgtta gagggccgc 
      gaacgtacc gcgtgaata ttttttttc gagcagttg 
      tactttcaa cgactccaa aattccggt accgacgag 
      aaataagga ccataccgg ccatcacgg taacggtga 
      gatgaacaa caagaggaa aaacagggg agggccgct 
      gaggaccca gagggctag atctgtaat agcagaagg 
      tgtttacaa taatgcaca ttttatgca ttctgcgac 
      tctacaaat cggaaatgt tgttcgtat aaaaacggc 
      ttccggtgt agtatggga aggcgtgtt caagacatt 
      ttcgagcaa cttcgatgt cgtcctcga ggtggctgc 
      aaaagtcga caatccaaa cagcccaac tcaaaccct 
      attggcgga tagtttcgt cgataagtt aatttttaa 
      atcgggcga catatcaac gatggaagc acgcgatgt 
      tgacttgga gtaccccac aaaggacac gtttcaaac 
      agacacggc tatttcagc ccgtcttaa tggcaattc 
      gaataagga agagaaaga actcgaacc gtgggaacc 
      ctcttcggg gatcaacgg cggcgacaa catcccctt 
      agagctgga gaacgcaaa cggttggga cgccaatga 
      tatgtttcg gaaaaatga gcaagaata cggcgttac 
      tagtacttt tgaccaatc gttgaaggg ctacactgt 
      gccgttacg gagtgccga tacggccga ttatcgcga 
      ggccgaagc aaaagcggc acaaatcgc caacgaaat 
      cgagaatca accgcagta taaggcacg ccttgagct 
      cgaaaacgg cgatgaaga ggcggcgtt tgctgccgt 
      tgttcggcc gaatcaaga acctaacga taagtatat 
      tccgccggc gaaacgaaa gaatcaaaa ttcgggaaa 
      attggtgcg aagtacgcc accaccttc ctccacaca 
      aggcccctc ccccaaagg caacgctcc gcctccgat 
      gccctaccc aaacaacgt ccacgcaat gcacatgcc 
      cccgcccat gcatcaaat gcccaatca tggacggcc 
      gtcgcatac gcccccgca tgggtacaa ccaacccat 
      gcggccgcc gttgaacca gaatagggc acagatgaa 
      tggcgaggc gaagccgag gggtcctcg aggacaagt 
      gcctatgga acagatggc ggcggcgca gcaacaagt 
      taaacaacc gccaccacc acagccacc acaaccaca 
      acagccgcc gccacaaca accgacgcc gcctcaaca 
      gagtatatc gccacaaca gcaacagca gcaacaaca 
      acagcaaga tccggtgga gaaggtgac gaatacgtt 
      caagaagag tacattgaa tccgaatgc gaaggagtt 
      tgtgctcaa tccggcggc taaaccgtt ccagccacg 
      ttctccaag cacgccgtc agcgagccg cccccacac 
      cccccaaac cccctctca cagcccata catccctgc 
      aacagtgag cggtcccgt aggccaacc ccacatgcc 
      tgtcatgat gccggttgg ctacatgat gacaagtca 
      accccaata ccagccccc gccacaagg tagccgcgt 
      ccgtaaaat tccgatgag ccaaatgcg tacggacgt 
      caccagtca aatgcaagt cgctgcggc aacaggtca 
      accactcct agctccgcc cattcaaca attcgtcta 
      tccaccggg catgaatcc gcaaggata tcatcaaat 
      gcacaccct tcggatgta cgaaacccc gccccaaat 
      ccaatacct cccacccaa tccatcgaa tacgccctc 
      gccggccca accgcccca atacacacc gggccaggg 
      cccccagcc gccgcccca acagtatca agccccgcc 
      tccgcaaag tcatccgca gtttcagcc tctcatgtg 
      ttccatact tcataccca gccgcacat gatgcaaaa 
      tatgcagta cctgcagca agccccgcc tccgcaaca 
      tcccattcc ggttatgct ccccccaca acacccaca 
      agcgcaaca gcatgggcc ggcccctta gcggtaatc 
      cacgtgttg tgttctagg taattttca ttggtccct 
      aatcccacc cactcccta ctcccgcaa acgttagtt 
      ttttttgtt ttttttcgg ttggtaata ccctaccta 
      cgagtgatt gttacagac ggagtcaaa attaatctt 
      tttttactt ttaggtaat taatatcta tgacaagta 
      aaaaattta atcagttgc tttatcgtg ttggtgaca 
      ctgtggtgc aattttacc aactttatt aaaaaccat 
      ggtaaaaca atcaatttt tttttcata ttttcttca 
      cccatttaa ttgactgtt aatatacca agcttcaaa 
      aaatttttt tcaaagcga ctatttatt attattaat 
      tgtgtgaga gtgtacagc gtgtgacag aatataggc 
      gtatggtac agtaagcga cccatttat ccctaagct 
      tggatattg acatggtcc ctcatgacg tctcattca 
      ttgcttcag tcgtctgta gtccggtta tttttttgt 
      tgtttcaaa attttgttg ttttttatt atttgttgt 
      acgttgtgt tgttgaggc atttctact cgaacattc 
      attcattcc ataagcagg tcatcgtat tatttcatt 
      tgttttcag caatgaaaa aaaatcgtc cctgttgcg 
      attattgat ttgtaagat atggggacg atcctactt 
      gaaaggtgc cttaaataa aaaatttgt gttttttca 
      tcacgtgcg atgcaatct ttggcacct gaaacattt 
      ttcgatact acgtgtttc tcaaaaata acaatgatt 
      caatttttt tttgttttt ttttgtata agatcaaaa 
      ttatttaaa ttttttata gattaagcg attgaatat 
      ttaaagctg tgcagaata ttttttaat agatggtca 
      actcagtta aaaaatctg tagagctgt gaatatttt 
      cagtgcacc atgttggtg cagaagtaa gtattgtca 
      aaaaaaagc agaaaagca gttaacagt tgtatgatt 
      ttcgcaaat aaagtcact ttggtttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY