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Gene information
General information
TC015827 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9021392..9025256
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015827
    iBeetle screen iB_08574

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 prothorax musculature dorsal pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    prothorax musculature specific subset dorsal oblique rounded - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015827 RA
      MSGAKPWER PWSMDEIIQ NSDNWNLAG DVALLNTLK 
      AFADNLLTR TTEINNNLQ NLTSSLSET SLQLNIAQN 
      EFQSLRNSQ FIESRVYED DETLEEPVE AKAEEKEVD 
      KTEEIRLAA LKGLEVLET YFEKVEVSV SDSEDDDID 
      LPNFVLRPK DLYLERPLP YVIGSEEWQ KTWHVGLED 
      SSSDSETEK VSDNFSESD SESELPIIP DRTQIKGTS 
      DTSSEDLRT EPKNFVNSS DSENSVPVQ PTTNFAEEL 
      AAKLGNVID QEYVNRRPI QKTPQYDNL FFDEPPPLD 
      EPKGLFSGG KGLFDDDDE ATPPTPKPE LRQPPKQDL 
      FDSDDDDIF TKKTTSYLQ NPVKMPLFS DEPPALDDK 
      KVAKPVGGV SIFGGGDLF DKKLLRRQP SSDEEENEK 
      SSPKGRGVN LFEEEPSDE PLVPDDHKF VKPVGGVSI 
      FGGGNLFDK KLLRRQPSS SDDEQNEKS PQKGRSVNL 
      FEEEPSVKP KKVTLFDDD DDLFKDDLF SSNATRKFT 
      SGLFDDDLF APTKPETKN STDLFADLM AIPDDKNSI 
      NVDRRETQK EAESPKPKP RLSLFEPDD LFASTSAKA 
      LFDPSPPPD HETTNQGEG PPPDHETTN QGEGPPPDH 
      ETPNQVQEE ESVEKPPPL DANKTNKPG KLKHNLNIN 
      VGALRPGSF PPKQTDGAA NATEAPPRD HHTSFDEAD 
      NVTVLPSVT KDRARRPLN RRPSTRKGR KEALKKFEQ 
      ANSTSRDPI LEVKSDKND SNSAQNRLE EPPDDLFET 
      GSQKTGIFD ESRDTFAVK TQPNLALFD EPPDDDFFE 
      KNDTKENPG LFDETSDDL FDIKRNSPE KDLNRSLGL 
      FEEPPDDLF ESRDDIFKV KSETKPSRD DIFEVKSES 
      KRTRDDIFG FKSETTSSR EIFEDKSDT KLSRDDIFE 
      VKSETKPSR DDIFEVKSE TKQSRDIFE AKSETSSHD 
      IFEEPPDLF ESTTEKGVK SEPKADLGL FDEVSDDDL 
      FGTKVESEV KPVESRDIP KIQSSQVKS DIFGDEDDD 
      DDLFNVKKM PIQSNKAKK SLFDDEDDD DIFKSFSSA 
      SVKKPETKV ATEKKVPKV STIKKLENT KPDDDPLSA 
      LLQ
    CDS sequence
    • >TC015827 RA
      atgtccggg gccaagccg tgggagcga ccatggtcc 
      atggacgaa ataatccaa aattcggac aattggaat 
      ttagccgga gatgtggcc cttttgaac actctcaag 
      gcttttgca gacaatctc ctgactcga acaaccgaa 
      atcaacaat aacttgcaa aatttgaca agctccctg 
      tctgagaca agtctccag ttgaatata gcccaaaac 
      gagtttcaa agtttgcga aacagccag tttattgaa 
      agtcgggtt tatgaggac gatgagact ttggaggag 
      ccggttgag gcgaaagcc gaggagaaa gaggttgat 
      aagactgag gaaatcagg ttggcggct ttgaagggt 
      ttggaggtc ttggagacg tattttgag aaggttgag 
      gtgtcggtc agtgacagt gaagacgac gatatcgac 
      ttgcccaat tttgtcctc agacccaaa gacttgtac 
      ctggagcgc ccccttccg tacgtaatc ggctcagaa 
      gagtggcaa aagacgtgg cacgtgggc ctggaggac 
      tcgtccagc gatagcgaa actgagaaa gtctcagat 
      aatttcagt gaaagcgac tcagaatca gagttgcca 
      attattccc gacagaaca cagatcaag ggcacctct 
      gataccagt tctgaggac ttgcgaact gaaccgaaa 
      aatttcgtc aattcgagt gatagtgaa aactcagtt 
      ccggtgcaa ccaactacc aactttgcc gaagagttg 
      gcggcgaaa ttggggaac gtgattgat caagagtac 
      gtcaatcga cggccaatc cagaaaacg ccacaatac 
      gataaccta tttttcgat gaaccgccg cctttggac 
      gaacctaag gggttgttt tcagggggt aagggcttg 
      tttgatgac gatgatgaa gccacgccc ccaaccccc 
      aaacccgaa ttgagacaa cccccaaaa caggacttg 
      ttcgatagc gatgatgat gatattttt acgaaaaaa 
      accacctct tatttacaa aatcctgtc aaaatgccc 
      ttgtttagc gacgagccg ccggcccta gacgacaag 
      aaggtggct aaaccagtg ggcggggtt tcaattttt 
      gggggcggc gacttgttc gataaaaaa ttattaagg 
      cggcagcca agctccgac gaggaggaa aacgaaaaa 
      tcgtcaccc aaggggcgg ggcgtcaat ttgtttgag 
      gaggagcca agtgatgag cctctagtt cccgacgac 
      cataagttt gttaaacca gtgggcgga gtttcaatt 
      ttcggcggt ggcaacttg ttcgataaa aaattgttg 
      aggcgacag ccaagctcc agtgacgat gagcaaaac 
      gaaaaatca ccacaaaaa gggcggagc gtcaattta 
      tttgaggag gagccaagt gtcaaaccg aaaaaagtg 
      acattgttt gatgacgac gatgactta tttaaagac 
      gatcttttc tcgtcaaat gcgacaaga aagttcact 
      tctggtctt ttcgatgac gatttattt gcaccaaca 
      aaaccagaa acgaaaaat tccaccgat ttattcgcc 
      gatttaatg gcaattcca gacgataaa aattccatt 
      aatgtcgat aggcgtgaa acgcaaaaa gaggcggag 
      tctccaaaa cctaaacca cgcctttcg ctatttgaa 
      cctgatgat ttgttcgcg tcaacgtcg gctaaagca 
      ctttttgat cctagccca ccccctgac cacgaaaca 
      accaatcag ggtgaaggc ccgccccct gaccacgaa 
      acaaccaat cagggtgaa ggcccgccc cctgaccac 
      gaaaccccc aatcaggtt caagaggag gaaagcgtg 
      gagaaaccg cccccgctt gacgcaaac aagactaac 
      aaaccagga aaactcaaa cacaatttg aatatcaac 
      gttggggcc ttaaggccg ggctcattt ccgccaaaa 
      cagacagat ggcgcagcg aacgccact gaagcgcca 
      ccacgtgac catcacacg tcatttgat gaggctgac 
      aacgttaca gtgttacca agtgtcact aaagatagg 
      gccagacga cccctaaat aggcgcccg tcaacacga 
      aaaggtcga aaagaagcc ttaaaaaaa tttgagcaa 
      gcaaatagc acgtcacgt gaccccata cttgaggtc 
      aagtcggac aaaaatgac tcaaattcg gctcaaaat 
      cggcttgaa gagccccct gatgattta ttcgaaact 
      gggtcacaa aaaaccggt atttttgat gagtcacgt 
      gacactttc gcggttaaa acccaaccg aatttggct 
      ttgtttgac gaacctccc gacgatgat tttttcgaa 
      aaaaatgac accaaagaa aatcctggt ctttttgat 
      gagacaagc gacgatcta tttgatata aagcgtaac 
      tcacccgaa aaagactta aatcgaagt cttggcttg 
      tttgaggag ccccccgac gacttattt gagtcacgt 
      gacgacatt tttaaggtc aaaagtgag actaaacca 
      tcacgtgat gacattttt gaggttaaa agtgagagt 
      aaacggaca cgtgacgac atttttggg ttcaaaagt 
      gagactaca tcgtcacgt gaaattttt gaggacaaa 
      agtgatact aaactgtca cgtgacgac atttttgag 
      gttaaaagt gagactaaa ccgtcacgt gacgacatt 
      tttgaggtc aagagtgaa actaaacag tcacgtgat 
      atttttgaa gctaaaagt gagacatcg tcacatgat 
      atttttgag gaacctcca gatttattt gagtctacg 
      accgaaaag ggtgttaaa agtgaaccc aaagcggac 
      ttaggattg tttgatgaa gtttcagac gatgatttg 
      ttcggtact aaagtcgaa agtgaggtt aagcctgtt 
      gagtcacgt gacattcca aaaatacag tcaagtcaa 
      gtcaaaagc gacattttc ggtgatgaa gacgacgat 
      gatgattta tttaacgtg aaaaaaatg ccaattcaa 
      tcaaataaa gctaaaaag tcgctattt gacgacgag 
      gatgatgat gatattttc aaaagtttt tctagtgcg 
      agtgttaaa aaaccggag actaaagtt gcgacggag 
      aaaaaagtt cctaaagtg agtaccata aaaaaatta 
      gagaatacg aagccagac gatgacccc ttatcagca 
      cttttacaa taa
    mRNA sequence
    • >TC015827 RA
      tttttgaca gatgtcaaa ttattatta ttattaatg 
      aaaaatagc aattacagg cctgttcag cgctttaga 
      aatgtccgg ggccaagcc gtgggagcg accatggtc 
      catggacga aataatcca aaattcgga caattggaa 
      tttagccgg agatgtggc ccttttgaa cactctcaa 
      ggcttttgc agacaatct cctgactcg aacaaccga 
      aatcaacaa taacttgca aaatttgac aagctccct 
      gtctgagac aagtctcca gttgaatat agcccaaaa 
      cgagtttca aagtttgcg aaacagcca gtttattga 
      aagtcgggt ttatgagga cgatgagac tttggagga 
      gccggttga ggcgaaagc cgaggagaa agaggttga 
      taagactga ggaaatcag gttggcggc tttgaaggg 
      tttggaggt cttggagac gtattttga gaaggttga 
      ggtgtcggt cagtgacag tgaagacga cgatatcga 
      cttgcccaa ttttgtcct cagacccaa agacttgta 
      cctggagcg cccccttcc gtacgtaat cggctcaga 
      agagtggca aaagacgtg gcacgtggg cctggagga 
      ctcgtccag cgatagcga aactgagaa agtctcaga 
      taatttcag tgaaagcga ctcagaatc agagttgcc 
      aattattcc cgacagaac acagatcaa gggcacctc 
      tgataccag ttctgagga cttgcgaac tgaaccgaa 
      aaatttcgt caattcgag tgatagtga aaactcagt 
      tccggtgca accaactac caactttgc cgaagagtt 
      ggcggcgaa attggggaa cgtgattga tcaagagta 
      cgtcaatcg acggccaat ccagaaaac gccacaata 
      cgataacct atttttcga tgaaccgcc gcctttgga 
      cgaacctaa ggggttgtt ttcaggggg taagggctt 
      gtttgatga cgatgatga agccacgcc cccaacccc 
      caaacccga attgagaca acccccaaa acaggactt 
      gttcgatag cgatgatga tgatatttt tacgaaaaa 
      aaccacctc ttatttaca aaatcctgt caaaatgcc 
      cttgtttag cgacgagcc gccggccct agacgacaa 
      gaaggtggc taaaccagt gggcggggt ttcaatttt 
      tgggggcgg cgacttgtt cgataaaaa attattaag 
      gcggcagcc aagctccga cgaggagga aaacgaaaa 
      atcgtcacc caaggggcg gggcgtcaa tttgtttga 
      ggaggagcc aagtgatga gcctctagt tcccgacga 
      ccataagtt tgttaaacc agtgggcgg agtttcaat 
      tttcggcgg tggcaactt gttcgataa aaaattgtt 
      gaggcgaca gccaagctc cagtgacga tgagcaaaa 
      cgaaaaatc accacaaaa agggcggag cgtcaattt 
      atttgagga ggagccaag tgtcaaacc gaaaaaagt 
      gacattgtt tgatgacga cgatgactt atttaaaga 
      cgatctttt ctcgtcaaa tgcgacaag aaagttcac 
      ttctggtct tttcgatga cgatttatt tgcaccaac 
      aaaaccaga aacgaaaaa ttccaccga tttattcgc 
      cgatttaat ggcaattcc agacgataa aaattccat 
      taatgtcga taggcgtga aacgcaaaa agaggcgga 
      gtctccaaa acctaaacc acgcctttc gctatttga 
      acctgatga tttgttcgc gtcaacgtc ggctaaagc 
      actttttga tcctagccc accccctga ccacgaaac 
      aaccaatca gggtgaagg cccgccccc tgaccacga 
      aacaaccaa tcagggtga aggcccgcc ccctgacca 
      cgaaacccc caatcaggt tcaagagga ggaaagcgt 
      ggagaaacc gcccccgct tgacgcaaa caagactaa 
      caaaccagg aaaactcaa acacaattt gaatatcaa 
      cgttggggc cttaaggcc gggctcatt tccgccaaa 
      acagacaga tggcgcagc gaacgccac tgaagcgcc 
      accacgtga ccatcacac gtcatttga tgaggctga 
      caacgttac agtgttacc aagtgtcac taaagatag 
      ggccagacg acccctaaa taggcgccc gtcaacacg 
      aaaaggtcg aaaagaagc cttaaaaaa atttgagca 
      agcaaatag cacgtcacg tgaccccat acttgaggt 
      caagtcgga caaaaatga ctcaaattc ggctcaaaa 
      tcggcttga agagccccc tgatgattt attcgaaac 
      tgggtcaca aaaaaccgg tatttttga tgagtcacg 
      tgacacttt cgcggttaa aacccaacc gaatttggc 
      tttgtttga cgaacctcc cgacgatga ttttttcga 
      aaaaaatga caccaaaga aaatcctgg tctttttga 
      tgagacaag cgacgatct atttgatat aaagcgtaa 
      ctcacccga aaaagactt aaatcgaag tcttggctt 
      gtttgagga gccccccga cgacttatt tgagtcacg 
      tgacgacat ttttaaggt caaaagtga gactaaacc 
      atcacgtga tgacatttt tgaggttaa aagtgagag 
      taaacggac acgtgacga catttttgg gttcaaaag 
      tgagactac atcgtcacg tgaaatttt tgaggacaa 
      aagtgatac taaactgtc acgtgacga catttttga 
      ggttaaaag tgagactaa accgtcacg tgacgacat 
      ttttgaggt caagagtga aactaaaca gtcacgtga 
      tatttttga agctaaaag tgagacatc gtcacatga 
      tatttttga ggaacctcc agatttatt tgagtctac 
      gaccgaaaa gggtgttaa aagtgaacc caaagcgga 
      cttaggatt gtttgatga agtttcaga cgatgattt 
      gttcggtac taaagtcga aagtgaggt taagcctgt 
      tgagtcacg tgacattcc aaaaataca gtcaagtca 
      agtcaaaag cgacatttt cggtgatga agacgacga 
      tgatgattt atttaacgt gaaaaaaat gccaattca 
      atcaaataa agctaaaaa gtcgctatt tgacgacga 
      ggatgatga tgatatttt caaaagttt ttctagtgc 
      gagtgttaa aaaaccgga gactaaagt tgcgacgga 
      gaaaaaagt tcctaaagt gagtaccat aaaaaaatt 
      agagaatac gaagccaga cgatgaccc cttatcagc 
      acttttaca ataatgggt tcatctcaa acatttatt 
      ttttgtaat gaaatcaca catgaaggt cataatcac 
      gctaaaaat tgactaaaa agtgaataa accgtaaat 
      aatact
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