Status
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Gene information
General information
TC004733 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4129114..4132057
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004733
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08559

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_11009

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004733 RA
      MCDKLISVV AFLASAILV AESISIHQT 
      YEGYAVGPK LRQHEKEQQ QDLSKIPGI 
      PGKDYPLFH SVPPTSFSC KHVPAHPGI 
      YANVETGCQ AYHVCHDGR EGEQGASFL 
      CTNGTIFNQ AEFACDWWY NVNCHEAPN 
      LYRLNLDPA KNPYVPKRK PEEELHFVP 
      VEKY
    • >TC004733 RB
      MFVLFFISA LSASTVATG LTTSGKQKR 
      QLFREHLLP QHGGKIPDG AFDPHRISL 
      NKLSQNGIF SPDGYHLAQ RHPDRHHNP 
      KQHLPNTLK VHHYPDNRF VAPQGQQHG 
      VQTIDTTYL IREPVQYPL FPPLRHYTP 
      LPFFPQYSL KLLRHFKYD KVNDHHDGS 
      WNEIELPTK ILKDFAPYL HKLHETD
    • >TC004733 RC
      MFVLFFISA LSASTVATG LTTSGKQKR QLFREHLLP 
      QHGGKIPDG AFDPHRISL NKLSQNGIF SPDGYHLAQ 
      RHPDRHHNP KQHLPNTLK VHHYPDNRF VAPQGQQHG 
      VQTIDTTYL IREPVQYPL FPPLRHYTP LPFFPQYSL 
      KLLRHFKYD KVNDHHDGS WNEIELPTK HQTYEGYAV 
      GPKLRQHEK EQQQDLSKI PGIPGKDYP LFHSVPPTS 
      FSCKHVPAH PGIYANVET GCQAYHVCH DGREGEQGA 
      SFLCTNGTI FNQAEFACD WWYNVNCHE APNLYRLNL 
      DPAKNPYVP KRKPEEELH FVPVEKY
    CDS sequence
    • >TC004733 RA
      atgtgcgat aaattaatt agtgttgtt gctttctta 
      gcttcagct atcctagta gctgagtca atctcgatt 
      caccaaacg tatgagggc tacgccgtg ggccccaag 
      ctccgtcag catgaaaag gagcaacag caggacttg 
      agcaaaatc ccgggaatc cccggcaaa gactatccc 
      cttttccac agcgtcccc ccgactagc ttctcctgc 
      aagcacgtt cctgcccat cctgggatt tacgcaaac 
      gttgaaacc ggctgccaa gcttaccac gtctgtcat 
      gatggtcgc gagggggag caaggagcc tccttcctc 
      tgcaccaac ggaaccatc ttcaaccaa gcggagttc 
      gcttgtgac tggtggtac aatgtcaac tgccacgaa 
      gccccaaat ctgtacaga ctcaatttg gaccccgcc 
      aaaaacccc tacgtcccc aaacgcaag cccgaggag 
      gagctccat ttcgtgccc gttgagaaa tattaa
    • >TC004733 RB
      atgttcgtt ctatttttc ataagtgcc ctctcggcc 
      tccacggtc gcgacagga ttgaccaca tcgggaaaa 
      caaaaacgg caattgttc cgcgaacat ctccttccg 
      cagcacggg ggtaaaatc cccgacggg gctttcgac 
      ccccatcgc atcagtttg aataagctg agccagaac 
      ggtattttt agccccgac ggctaccac cttgcccaa 
      agacacccc gaccggcac cacaacccc aaacagcac 
      ctccccaac accttgaag gtgcatcat tatccagac 
      aataggttt gttgcgcct caggggcaa cagcacggg 
      gtccaaacc attgacacc acctacctc atacgcgaa 
      ccagtacaa taccccctt ttcccgccc ttaagacat 
      tacacccct ttaccgttt ttcccgcag tattctctc 
      aaattgctc agacatttc aaatatgac aaagttaat 
      gaccatcac gacggctcc tggaacgaa atcgaattg 
      ccaacgaag atattaaaa gattttgct ccgtatctc 
      cacaagtta catgagact gactga
    • >TC004733 RC
      atgttcgtt ctatttttc ataagtgcc ctctcggcc 
      tccacggtc gcgacagga ttgaccaca tcgggaaaa 
      caaaaacgg caattgttc cgcgaacat ctccttccg 
      cagcacggg ggtaaaatc cccgacggg gctttcgac 
      ccccatcgc atcagtttg aataagctg agccagaac 
      ggtattttt agccccgac ggctaccac cttgcccaa 
      agacacccc gaccggcac cacaacccc aaacagcac 
      ctccccaac accttgaag gtgcatcat tatccagac 
      aataggttt gttgcgcct caggggcaa cagcacggg 
      gtccaaacc attgacacc acctacctc atacgcgaa 
      ccagtacaa taccccctt ttcccgccc ttaagacat 
      tacacccct ttaccgttt ttcccgcag tattctctc 
      aaattgctc agacatttc aaatatgac aaagttaat 
      gaccatcac gacggctcc tggaacgaa atcgaattg 
      ccaacgaag caccaaacg tatgagggc tacgccgtg 
      ggccccaag ctccgtcag catgaaaag gagcaacag 
      caggacttg agcaaaatc ccgggaatc cccggcaaa 
      gactatccc cttttccac agcgtcccc ccgactagc 
      ttctcctgc aagcacgtt cctgcccat cctgggatt 
      tacgcaaac gttgaaacc ggctgccaa gcttaccac 
      gtctgtcat gatggtcgc gagggggag caaggagcc 
      tccttcctc tgcaccaac ggaaccatc ttcaaccaa 
      gcggagttc gcttgtgac tggtggtac aatgtcaac 
      tgccacgaa gccccaaat ctgtacaga ctcaatttg 
      gaccccgcc aaaaacccc tacgtcccc aaacgcaag 
      cccgaggag gagctccat ttcgtgccc gttgagaaa 
      tattaa
    mRNA sequence
    • >TC004733 RA
      agtctcaaa ttcacagtt acgcccaag tgttgtgta 
      acacaatgc caatttttg agacaatat ttacataga 
      aaaagttgt gacactcga aaaaattag tctatagcc 
      acatagtgc gagataaag caaaactga gaatttcta 
      gtggaccca aagttgtat ttaattaaa tttggtcca 
      ctaagtagg gaaagtgat atatgacct gttccactc 
      ttgctcttg cagaattgt gatcattcg agacctgcc 
      agtgtgaga gtgtgttga ataatgtgc gataaatta 
      attagtgtt gttgctttc ttagcttca gctatccta 
      gtagctgag tcaatctcg attcaccaa acgtatgag 
      ggctacgcc gtgggcccc aagctccgt cagcatgaa 
      aaggagcaa cagcaggac ttgagcaaa atcccggga 
      atccccggc aaagactat ccccttttc cacagcgtc 
      cccccgact agcttctcc tgcaagcac gttcctgcc 
      catcctggg atttacgca aacgttgaa accggctgc 
      caagcttac cacgtctgt catgatggt cgcgagggg 
      gagcaagga gcctccttc ctctgcacc aacggaacc 
      atcttcaac caagcggag ttcgcttgt gactggtgg 
      tacaatgtc aactgccac gaagcccca aatctgtac 
      agactcaat ttggacccc gccaaaaac ccctacgtc 
      cccaaacgc aagcccgag gaggagctc catttcgtg 
      cccgttgag aaatattaa tgttttgta aatgtttcg 
      tttaaataa ataaattat tgttta
    • >TC004733 RB
      gttaaatct cttgaatgg cggagtggt ggaacaaat 
      tccataatc tcgcctcac aatagtctt agtgctctt 
      agtacatct ttaagtttt ccagtggaa gtgatatat 
      tggcttctt tggtgtcat agtgtgtgt gtgaaaatg 
      gcaatttcg tcaacgact gattggtca aaaatccgt 
      tacatcatg ttcgttcta tttttcata agtgccctc 
      tcggcctcc acggtcgcg acaggattg accacatcg 
      ggaaaacaa aaacggcaa ttgttccgc gaacatctc 
      cttccgcag cacgggggt aaaatcccc gacggggct 
      ttcgacccc catcgcatc agtttgaat aagctgagc 
      cagaacggt atttttagc cccgacggc taccacctt 
      gcccaaaga caccccgac cggcaccac aaccccaaa 
      cagcacctc cccaacacc ttgaaggtg catcattat 
      ccagacaat aggtttgtt gcgcctcag gggcaacag 
      cacggggtc caaaccatt gacaccacc tacctcata 
      cgcgaacca gtacaatac ccccttttc ccgccctta 
      agacattac accccttta ccgtttttc ccgcagtat 
      tctctcaaa ttgctcaga catttcaaa tatgacaaa 
      gttaatgac catcacgac ggctcctgg aacgaaatc 
      gaattgcca acgaagata ttaaaagat tttgctccg 
      tatctccac aagttacat gagactgac tgaggacat 
      taagcagcg gccacatta tgacgttca caattgtat 
      tttacatat ttctatgaa aataaaaat atagcgaaa 
      ag
    • >TC004733 RC
      gttaaatct cttgaatgg cggagtggt ggaacaaat 
      tccataatc tcgcctcac aatagtctt agtgctctt 
      agtacatct ttaagtttt ccagtggaa gtgatatat 
      tggcttctt tggtgtcat agtgtgtgt gtgaaaatg 
      gcaatttcg tcaacgact gattggtca aaaatccgt 
      tacatcatg ttcgttcta tttttcata agtgccctc 
      tcggcctcc acggtcgcg acaggattg accacatcg 
      ggaaaacaa aaacggcaa ttgttccgc gaacatctc 
      cttccgcag cacgggggt aaaatcccc gacggggct 
      ttcgacccc catcgcatc agtttgaat aagctgagc 
      cagaacggt atttttagc cccgacggc taccacctt 
      gcccaaaga caccccgac cggcaccac aaccccaaa 
      cagcacctc cccaacacc ttgaaggtg catcattat 
      ccagacaat aggtttgtt gcgcctcag gggcaacag 
      cacggggtc caaaccatt gacaccacc tacctcata 
      cgcgaacca gtacaatac ccccttttc ccgccctta 
      agacattac accccttta ccgtttttc ccgcagtat 
      tctctcaaa ttgctcaga catttcaaa tatgacaaa 
      gttaatgac catcacgac ggctcctgg aacgaaatc 
      gaattgcca acgaagcac caaacgtat gagggctac 
      gccgtgggc cccaagctc cgtcagcat gaaaaggag 
      caacagcag gacttgagc aaaatcccg ggaatcccc 
      ggcaaagac tatcccctt ttccacagc gtccccccg 
      actagcttc tcctgcaag cacgttcct gcccatcct 
      gggatttac gcaaacgtt gaaaccggc tgccaagct 
      taccacgtc tgtcatgat ggtcgcgag ggggagcaa 
      ggagcctcc ttcctctgc accaacgga accatcttc 
      aaccaagcg gagttcgct tgtgactgg tggtacaat 
      gtcaactgc cacgaagcc ccaaatctg tacagactc 
      aatttggac cccgccaaa aacccctac gtccccaaa 
      cgcaagccc gaggaggag ctccatttc gtgcccgtt 
      gagaaatat taatgtttt gtaaatgtt tcgtttaaa 
      taaataaat tattgttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY