Status
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Gene information
General information
TC015731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5810427..5815691
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015731
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08553

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015731 RA
      MKKDFVCNQ KLLVEKMGY FAEVTTGQR LEDMDISVH 
      CDIGIFEWL MKWVKKESL LEEDWPQLD PQCVIPILV 
      SAAFLQMEP LQNDCLLYC HQHMNEILR TSTNLSCLN 
      DSILTRLAA MYTNSEVEA IKDRKDKIQ SRIFTKLIQ 
      SLAEPEPES MRGHWSSLA RVYRCGRCQ QLIGPSVAT 
      RIPCAPPCM QLQCDGSII SQHVRDPSW NINDYILKL 
      YKNLKTWRK VYWRLWGDS HFLYCLTYP PQYFTLDAQ 
      KAPLPVGRY PCCGERAYR FQLLQDYSG CQFREHTIS 
      AKDVRDSAV LSLLENFRG LIEEEPPQL MFPERLTRL 
      VARDPAGDS KKLVCKENF WWEGFQIVP PRPKLGLLG 
      QFASRGGEE AVPEEESSE GTDESTSDS DDSDSEGSD 
      GSAESNPPP MPVKNLKKK RNKRESRLW QHNLSARSN 
      QDIQRAFEE NAVREITAM LNRRTVTIS EGGAKSGRF 
      SRNVTPIGG VWVRLESEW KEVHCANNN QQKMRMRLL 
      EINNKMVKK RYNWKSRQV VNTVVDNSA TKKIQLDLQ 
      VEGHYDQSN ELVLPSKKR KTKIKDEKQ KITKILSKK 
      HRKKLEKIV EKKKKKENR ASLLEALSK VQATPEELT 
      KLTSIATVQ TKGLKRHYL EEQTGDKKK PKRDLQIIS 
      GEIKLNSLS GAKRRQLLE QTVDTKPNG DPNVVGFES 
      SDSGNDSDP EPTKNEPTE TEPEPKEPP IKNEPKETK 
      TKTPTRKVL APKPAVFVD VIRDEEIQN SRLKLPILA 
      EEQQIMETI NENPVVIIA GETGSGKTT QVPQFLYEA 
      GYALKKQIA VTEPRRVAA IAMSKRVAQ EMNLSSNEV 
      SYLIRFEGN ATEDTKIKF MTDGVLLKE VQNDFLLSQ 
      YSVVILDEA HERSVYTDI LIGLLSRIV PLRVKRGDP 
      LKLIIMSAT LRVEDFTKN KRLFKKTPP VINVDSRQF 
      PVTVHFNKR TNEDYLSES FTKVVKIHT KLPEGGVLV 
      FVTGQQEVN SLVKKLRAK FPFKVKLNE EAEPTKLKQ 
      KKVYAPEVN LDDYDPDWS SESEEEESE RPLYHAPPL 
      WVLPLYSML PTHKQNRVF QAPPPGCRL CVVSTNVAE 
      TSLTIPNIK YVVDSGRTK VKLYDKITG VSSYVVTWT 
      SKASANQRA GRAGRTGPG HCYRLYSSA VFNDTLHDF 
      CVPEIQQKP VDDLYLQMK CMSIDKVVN FPFPTAPDL 
      LQLKTAEHR LEILGALQN SQVTPLGRA IAKFPVLPR 
      FGKMLALSH QQDLLPYTI CMVAALSVQ EVLLDDAKW 
      AALRRQWAA TGNSLLLGD SMVLLRAVG AAEFANTQN 
      RLEQFCHAN GLRHKAVLE IRKLRLQLT NEIKANVPE 
      ADVVVDPAL APPTDLQAK LLRQILLAG MGDQIAKKI 
      ESEDKKFKY GYQANNMED PVFLHSGSV LRKNLPEFV 
      IYHEIYETN KIYMRGVTA IEPEWLPVY VPSLCNLSE 
      PLAEPEPRL KNGKVYCTV NGTFGSQGW TLPNVEIEY 
      PRTINYYKW FGRFLLEGL VFKKLEKYK DVLLSQPAI 
      MVKSWAKLQ PRTDAILKA LLSKEAVSK QRLAEIWTD 
      TPKCGCRSR PMEK
    CDS sequence
    • >TC015731 RA
      atgaagaaa gacttcgtt tgcaaccag aaactcctt 
      gtggaaaaa atgggttat ttcgctgaa gtgaccacg 
      gggcaacgc ctcgaagac atggacatt tccgtccac 
      tgtgacatt ggtattttc gaatggttg atgaaatgg 
      gtgaagaaa gaatccttg cttgaggaa gactggccc 
      caattggac ccccaatgc gtcatcccg atcctcgtt 
      tctgcggcg tttcttcaa atggaaccg ctccaaaac 
      gactgcctt ttgtactgc caccaacac atgaacgaa 
      atcctccga acgtctacg aacctatcg tgtctcaac 
      gactcgatt ttgacacgc cttgccgcc atgtacacc 
      aatagcgaa gtggaggcg atcaaggac cgcaaagat 
      aagatccaa tcacgcatt ttcacgaaa ttgatccaa 
      tcgctggcg gagcctgag cctgaaagc atgcgtggc 
      cattggtcg tcactggcg agggtgtat cgctgtggg 
      cggtgccaa cagctgatt ggtccctcg gttgcgaca 
      cgtatacct tgtgccccg ccttgtatg caattacag 
      tgcgatggt tccataata agccaacac gtcagagac 
      ccatcatgg aacataaat gactatatt ttaaaactg 
      tacaagaat ttgaagact tggagaaaa gtttattgg 
      agattatgg ggcgactcc cactttttg tattgtctg 
      acgtatccg ccccaatat ttcaccctt gacgcccag 
      aaagcgcca ctccccgtt gggcgttac ccctgttgt 
      ggggagaga gcttatcgt ttccagtta ttacaagat 
      tattccggg tgtcagttc cgggaacat acaattagc 
      gcgaaagac gtgcgagat tcggccgtt ttgtcactt 
      ttggagaat ttccggggg ttaatagag gaggagcct 
      ccacagttg atgtttccg gaacgttta acccgattg 
      gttgcacgt gacccggcc ggcgatagc aagaagttg 
      gtttgtaaa gagaatttc tggtgggag gggtttcaa 
      attgtcccg cccaggccc aagctggga ctgttgggc 
      caatttgct agtcggggc ggggaagag gccgtgccg 
      gaggaggag tcaagtgaa gggacggac gaaagtacg 
      tccgacagt gatgatagt gacagtgag gggagcgac 
      ggaagtgcc gaaagtaat cctccacca atgcctgtc 
      aagaacctt aagaagaaa agaaataag cgggaaagt 
      aggttgtgg caacataat ttgtcggcg agaagtaac 
      caggatata caaagagcg tttgaggag aatgcggtt 
      agggaaata acggccatg ttgaatcgg cggacggtc 
      accatttcg gaaggcggg gccaaaagt gggcggttc 
      tcgcggaat gtcacgccc attggtggg gtttgggtg 
      cgattagag tccgaatgg aaggaagtc cattgcgct 
      aataataac cagcaaaaa atgcgaatg cgattgttg 
      gaaataaac aataaaatg gtcaaaaag cgctataat 
      tggaagtcg cgacaggtt gttaacacc gttgttgat 
      aattccgcg accaaaaag atccaactt gatttgcaa 
      gtggagggc cactatgac caaagtaat gagttagtg 
      ctgccttca aaaaaacgc aagacaaaa ataaaagat 
      gagaaacaa aaaatcacc aaaatcctt tcaaagaaa 
      cacagaaag aaacttgaa aaaatcgtc gagaaaaag 
      aagaaaaag gagaaccgc gcgtcactc cttgaagct 
      ttatcgaaa gtccaagca acccctgaa gagttaacc 
      aaactaacc tcaattgcc actgtacaa acaaagggg 
      ctaaaacgg cactatctc gaggagcaa accggagac 
      aagaagaaa cctaaacgc gacttgcag ataatttcg 
      ggcgaaatt aaactaaac agtttatcg ggggccaaa 
      agacgccaa ttgctggaa caaacagtt gatactaaa 
      ccaaatggg gaccccaac gtggtgggg ttcgagtcg 
      tcagactca ggcaatgac tcggacccc gagcccacg 
      aaaaatgaa ccaacagaa acagaacca gaaccaaaa 
      gaaccacca attaaaaac gaaccaaag gaaacaaaa 
      actaaaact ccaaccagg aaagttctt gcgccaaaa 
      cctgcagtt ttcgtggac gtgatccgc gacgaagaa 
      atccagaac agtcgcctc aagctcccg atcttggcc 
      gaggagcag caaatcatg gaaacgatt aacgaaaat 
      ccggttgtg ataatcgct ggtgagaca ggcagtggt 
      aaaaccact caagtcccc cagtttttg tacgaagcg 
      ggctatgcg ttgaaaaaa caaatcgcg gtgactgaa 
      ccccgaaga gttgcagcg attgcaatg tcgaaacgt 
      gtggcacaa gaaatgaat ttaagctcg aacgaagtg 
      agctattta attcggttc gagggcaac gccaccgaa 
      gacacaaaa attaagttt atgacagat ggcgtgtta 
      ctaaaagag gtccaaaac gattttctc ttgtcacaa 
      tattcagtt gttatcctt gatgaagcc cacgaaaga 
      tcggtttat accgatatt ttaatcggg cttttatca 
      cgcattgta cctttgcga gtcaaaagg ggggatccc 
      ctaaaactc ataatcatg tcggctacg ttacgagtc 
      gaagacttc acgaaaaat aagcgactt tttaagaaa 
      actccccct gtgattaac gtggatagt cggcaattc 
      cccgtcact gttcacttc aataaacgc acaaatgag 
      gattatttg agtgagagt ttcactaaa gtggttaaa 
      attcacacg aaactgccc gaaggcgga gttttggtt 
      ttcgtgact gggcaacaa gaagtcaac tcacttgtg 
      aaaaaactc cgcgctaaa ttccccttc aaagtcaag 
      cttaatgaa gaggcggag cctacaaaa ctcaaacag 
      aaaaaggtt tacgccccc gaggtgaat ctcgatgat 
      tacgacccg gattggtcg agtgagagt gaggaggag 
      gagtcggaa cgccccctg tatcacgcc ccgccgttg 
      tgggtgtta cccctctat tcgatgctc cccacccac 
      aagcaaaac cgagtgttt caagcgccg cccccagga 
      tgtcgtttg tgcgtcgtt agtaccaac gtagcggag 
      acaagtctc actatacct aatataaaa tacgtggtt 
      gactcaggt cggactaaa gtgaaactg tacgataaa 
      atcaccggt gtgagtagt tacgtggtc acgtggacc 
      agcaaagct tcggccaat cagcgcgcc gggagggcg 
      gggcgtacg gggccgggc cactgctac cgcctctat 
      tccagtgct gtttttaac gacacgtta cacgatttt 
      tgtgttccg gaaattcag caaaaacca gtcgatgat 
      ttatacttg caaatgaaa tgtatgagt atcgataaa 
      gtcgtaaac ttccccttc cccacagcc cccgactta 
      ttgcaattg aaaacagcc gaacaccga ctggaaata 
      ttgggggcg ttgcaaaac agccaagtc acgcctttg 
      gggcgtgcc atcgccaaa ttccccgtg ttgccacgt 
      ttcggcaaa atgttggcc ttgagtcac caacaagac 
      ctcctcccg tacaccata tgtatggtg gcagcactg 
      tcagtccag gaggtgttg ctagacgac gccaaatgg 
      gcggcgctt cgtcgccag tgggcggcg acgggcaat 
      tcgctccta ttgggcgac agtatggtg ctgctccgg 
      gcggtgggc gcggccgaa ttcgccaac acccaaaac 
      cgactggag caattctgt cacgccaat gggttgaga 
      cacaaggcg gtgctcgaa attcgaaag ctgcgccta 
      caattgacc aatgaaatc aaggctaat gtgccggag 
      gccgatgtt gtggtagac cccgccctt gccccgccc 
      actgacctt caagccaag cttctaaga cagatactt 
      ctcgcaggg atgggcgac caaatcgcc aagaaaatc 
      gaaagtgag gataagaaa tttaaatat ggataccag 
      gccaacaat atggaggac ccagtcttt ttacattcg 
      gggtctgta ttacgaaaa aatctgcca gagtttgtg 
      atttatcac gaaatttac gaaactaat aaaatttat 
      atgagaggg gtgacagcc attgaaccc gagtggttg 
      ccggtttat gtccccagt ttgtgcaat ttgagtgaa 
      cctttagcc gagcctgaa cctcgacta aaaaacgga 
      aaagtgtat tgtacggta aatggaacg tttggtagt 
      caaggctgg acgctaccg aacgttgaa atagaatat 
      cccagaacc attaattat tataagtgg tttggtcga 
      tttttattg gaaggtttg gtgtttaaa aaactggag 
      aaatacaag gacgtgttg ttgagtcag ccggcgatt 
      atggtcaaa agttgggcg aagttgcaa ccgcggacc 
      gacgctatt ttgaaagcc ttgctgtcg aaggaggcg 
      gtgtcgaag cagcgattg gcggagatt tggacggac 
      acgcccaag tgtgggtgc cgcagtcgg cctatggag 
      aagtga
    mRNA sequence
    • >TC015731 RA
      acgaggtga aaaacatga agaaagact tcgtttgca 
      accagaaac tccttgtgg aaaaaatgg gttatttcg 
      ctgaagtga ccacggggc aacgcctcg aagacatgg 
      acatttccg tccactgtg acattggta ttttcgaat 
      ggttgatga aatgggtga agaaagaat ccttgcttg 
      aggaagact ggccccaat tggaccccc aatgcgtca 
      tcccgatcc tcgtttctg cggcgtttc ttcaaatgg 
      aaccgctcc aaaacgact gccttttgt actgccacc 
      aacacatga acgaaatcc tccgaacgt ctacgaacc 
      tatcgtgtc tcaacgact cgattttga cacgccttg 
      ccgccatgt acaccaata gcgaagtgg aggcgatca 
      aggaccgca aagataaga tccaatcac gcattttca 
      cgaaattga tccaatcgc tggcggagc ctgagcctg 
      aaagcatgc gtggccatt ggtcgtcac tggcgaggg 
      tgtatcgct gtgggcggt gccaacagc tgattggtc 
      cctcggttg cgacacgta taccttgtg ccccgcctt 
      gtatgcaat tacagtgcg atggttcca taataagcc 
      aacacgtca gagacccat catggaaca taaatgact 
      atattttaa aactgtaca agaatttga agacttgga 
      gaaaagttt attggagat tatggggcg actcccact 
      ttttgtatt gtctgacgt atccgcccc aatatttca 
      cccttgacg cccagaaag cgccactcc ccgttgggc 
      gttacccct gttgtgggg agagagctt atcgtttcc 
      agttattac aagattatt ccgggtgtc agttccggg 
      aacatacaa ttagcgcga aagacgtgc gagattcgg 
      ccgttttgt cacttttgg agaatttcc gggggttaa 
      tagaggagg agcctccac agttgatgt ttccggaac 
      gtttaaccc gattggttg cacgtgacc cggccggcg 
      atagcaaga agttggttt gtaaagaga atttctggt 
      gggaggggt ttcaaattg tcccgccca ggcccaagc 
      tgggactgt tgggccaat ttgctagtc ggggcgggg 
      aagaggccg tgccggagg aggagtcaa gtgaaggga 
      cggacgaaa gtacgtccg acagtgatg atagtgaca 
      gtgagggga gcgacggaa gtgccgaaa gtaatcctc 
      caccaatgc ctgtcaaga accttaaga agaaaagaa 
      ataagcggg aaagtaggt tgtggcaac ataatttgt 
      cggcgagaa gtaaccagg atatacaaa gagcgtttg 
      aggagaatg cggttaggg aaataacgg ccatgttga 
      atcggcgga cggtcacca tttcggaag gcggggcca 
      aaagtgggc ggttctcgc ggaatgtca cgcccattg 
      gtggggttt gggtgcgat tagagtccg aatggaagg 
      aagtccatt gcgctaata ataaccagc aaaaaatgc 
      gaatgcgat tgttggaaa taaacaata aaatggtca 
      aaaagcgct ataattgga agtcgcgac aggttgtta 
      acaccgttg ttgataatt ccgcgacca aaaagatcc 
      aacttgatt tgcaagtgg agggccact atgaccaaa 
      gtaatgagt tagtgctgc cttcaaaaa aacgcaaga 
      caaaaataa aagatgaga aacaaaaaa tcaccaaaa 
      tcctttcaa agaaacaca gaaagaaac ttgaaaaaa 
      tcgtcgaga aaaagaaga aaaaggaga accgcgcgt 
      cactccttg aagctttat cgaaagtcc aagcaaccc 
      ctgaagagt taaccaaac taacctcaa ttgccactg 
      tacaaacaa aggggctaa aacggcact atctcgagg 
      agcaaaccg gagacaaga agaaaccta aacgcgact 
      tgcagataa tttcgggcg aaattaaac taaacagtt 
      tatcggggg ccaaaagac gccaattgc tggaacaaa 
      cagttgata ctaaaccaa atggggacc ccaacgtgg 
      tggggttcg agtcgtcag actcaggca atgactcgg 
      accccgagc ccacgaaaa atgaaccaa cagaaacag 
      aaccagaac caaaagaac caccaatta aaaacgaac 
      caaaggaaa caaaaacta aaactccaa ccaggaaag 
      ttcttgcgc caaaacctg cagttttcg tggacgtga 
      tccgcgacg aagaaatcc agaacagtc gcctcaagc 
      tcccgatct tggccgagg agcagcaaa tcatggaaa 
      cgattaacg aaaatccgg ttgtgataa tcgctggtg 
      agacaggca gtggtaaaa ccactcaag tcccccagt 
      ttttgtacg aagcgggct atgcgttga aaaaacaaa 
      tcgcggtga ctgaacccc gaagagttg cagcgattg 
      caatgtcga aacgtgtgg cacaagaaa tgaatttaa 
      gctcgaacg aagtgagct atttaattc ggttcgagg 
      gcaacgcca ccgaagaca caaaaatta agtttatga 
      cagatggcg tgttactaa aagaggtcc aaaacgatt 
      ttctcttgt cacaatatt cagttgtta tccttgatg 
      aagcccacg aaagatcgg tttataccg atattttaa 
      tcgggcttt tatcacgca ttgtacctt tgcgagtca 
      aaagggggg atcccctaa aactcataa tcatgtcgg 
      ctacgttac gagtcgaag acttcacga aaaataagc 
      gacttttta agaaaactc cccctgtga ttaacgtgg 
      atagtcggc aattccccg tcactgttc acttcaata 
      aacgcacaa atgaggatt atttgagtg agagtttca 
      ctaaagtgg ttaaaattc acacgaaac tgcccgaag 
      gcggagttt tggttttcg tgactgggc aacaagaag 
      tcaactcac ttgtgaaaa aactccgcg ctaaattcc 
      ccttcaaag tcaagctta atgaagagg cggagccta 
      caaaactca aacagaaaa aggtttacg cccccgagg 
      tgaatctcg atgattacg acccggatt ggtcgagtg 
      agagtgagg aggaggagt cggaacgcc ccctgtatc 
      acgccccgc cgttgtggg tgttacccc tctattcga 
      tgctcccca cccacaagc aaaaccgag tgtttcaag 
      cgccgcccc caggatgtc gtttgtgcg tcgttagta 
      ccaacgtag cggagacaa gtctcacta tacctaata 
      taaaatacg tggttgact caggtcgga ctaaagtga 
      aactgtacg ataaaatca ccggtgtga gtagttacg 
      tggtcacgt ggaccagca aagcttcgg ccaatcagc 
      gcgccggga gggcggggc gtacggggc cgggccact 
      gctaccgcc tctattcca gtgctgttt ttaacgaca 
      cgttacacg atttttgtg ttccggaaa ttcagcaaa 
      aaccagtcg atgatttat acttgcaaa tgaaatgta 
      tgagtatcg ataaagtcg taaacttcc ccttcccca 
      cagcccccg acttattgc aattgaaaa cagccgaac 
      accgactgg aaatattgg gggcgttgc aaaacagcc 
      aagtcacgc ctttggggc gtgccatcg ccaaattcc 
      ccgtgttgc cacgtttcg gcaaaatgt tggccttga 
      gtcaccaac aagacctcc tcccgtaca ccatatgta 
      tggtggcag cactgtcag tccaggagg tgttgctag 
      acgacgcca aatgggcgg cgcttcgtc gccagtggg 
      cggcgacgg gcaattcgc tcctattgg gcgacagta 
      tggtgctgc tccgggcgg tgggcgcgg ccgaattcg 
      ccaacaccc aaaaccgac tggagcaat tctgtcacg 
      ccaatgggt tgagacaca aggcggtgc tcgaaattc 
      gaaagctgc gcctacaat tgaccaatg aaatcaagg 
      ctaatgtgc cggaggccg atgttgtgg tagaccccg 
      cccttgccc cgcccactg accttcaag ccaagcttc 
      taagacaga tacttctcg cagggatgg gcgaccaaa 
      tcgccaaga aaatcgaaa gtgaggata agaaattta 
      aatatggat accaggcca acaatatgg aggacccag 
      tctttttac attcggggt ctgtattac gaaaaaatc 
      tgccagagt ttgtgattt atcacgaaa tttacgaaa 
      ctaataaaa tttatatga gaggggtga cagccattg 
      aacccgagt ggttgccgg tttatgtcc ccagtttgt 
      gcaatttga gtgaacctt tagccgagc ctgaacctc 
      gactaaaaa acggaaaag tgtattgta cggtaaatg 
      gaacgtttg gtagtcaag gctggacgc taccgaacg 
      ttgaaatag aatatccca gaaccatta attattata 
      agtggtttg gtcgatttt tattggaag gtttggtgt 
      ttaaaaaac tggagaaat acaaggacg tgttgttga 
      gtcagccgg cgattatgg tcaaaagtt gggcgaagt 
      tgcaaccgc ggaccgacg ctattttga aagccttgc 
      tgtcgaagg aggcggtgt cgaagcagc gattggcgg 
      agatttgga cggacacgc ccaagtgtg ggtgccgca 
      gtcggccta tggagaagt gagcatgat gtggccgcc 
      tttagatta at
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