| General information | |||
| TC015731 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 5810427..5815691 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015731 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08553 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015731 RA
MKKDFVCNQ KLLVEKMGY FAEVTTGQR LEDMDISVH CDIGIFEWL MKWVKKESL LEEDWPQLD PQCVIPILV SAAFLQMEP LQNDCLLYC HQHMNEILR TSTNLSCLN DSILTRLAA MYTNSEVEA IKDRKDKIQ SRIFTKLIQ SLAEPEPES MRGHWSSLA RVYRCGRCQ QLIGPSVAT RIPCAPPCM QLQCDGSII SQHVRDPSW NINDYILKL YKNLKTWRK VYWRLWGDS HFLYCLTYP PQYFTLDAQ KAPLPVGRY PCCGERAYR FQLLQDYSG CQFREHTIS AKDVRDSAV LSLLENFRG LIEEEPPQL MFPERLTRL VARDPAGDS KKLVCKENF WWEGFQIVP PRPKLGLLG QFASRGGEE AVPEEESSE GTDESTSDS DDSDSEGSD GSAESNPPP MPVKNLKKK RNKRESRLW QHNLSARSN QDIQRAFEE NAVREITAM LNRRTVTIS EGGAKSGRF SRNVTPIGG VWVRLESEW KEVHCANNN QQKMRMRLL EINNKMVKK RYNWKSRQV VNTVVDNSA TKKIQLDLQ VEGHYDQSN ELVLPSKKR KTKIKDEKQ KITKILSKK HRKKLEKIV EKKKKKENR ASLLEALSK VQATPEELT KLTSIATVQ TKGLKRHYL EEQTGDKKK PKRDLQIIS GEIKLNSLS GAKRRQLLE QTVDTKPNG DPNVVGFES SDSGNDSDP EPTKNEPTE TEPEPKEPP IKNEPKETK TKTPTRKVL APKPAVFVD VIRDEEIQN SRLKLPILA EEQQIMETI NENPVVIIA GETGSGKTT QVPQFLYEA GYALKKQIA VTEPRRVAA IAMSKRVAQ EMNLSSNEV SYLIRFEGN ATEDTKIKF MTDGVLLKE VQNDFLLSQ YSVVILDEA HERSVYTDI LIGLLSRIV PLRVKRGDP LKLIIMSAT LRVEDFTKN KRLFKKTPP VINVDSRQF PVTVHFNKR TNEDYLSES FTKVVKIHT KLPEGGVLV FVTGQQEVN SLVKKLRAK FPFKVKLNE EAEPTKLKQ KKVYAPEVN LDDYDPDWS SESEEEESE RPLYHAPPL WVLPLYSML PTHKQNRVF QAPPPGCRL CVVSTNVAE TSLTIPNIK YVVDSGRTK VKLYDKITG VSSYVVTWT SKASANQRA GRAGRTGPG HCYRLYSSA VFNDTLHDF CVPEIQQKP VDDLYLQMK CMSIDKVVN FPFPTAPDL LQLKTAEHR LEILGALQN SQVTPLGRA IAKFPVLPR FGKMLALSH QQDLLPYTI CMVAALSVQ EVLLDDAKW AALRRQWAA TGNSLLLGD SMVLLRAVG AAEFANTQN RLEQFCHAN GLRHKAVLE IRKLRLQLT NEIKANVPE ADVVVDPAL APPTDLQAK LLRQILLAG MGDQIAKKI ESEDKKFKY GYQANNMED PVFLHSGSV LRKNLPEFV IYHEIYETN KIYMRGVTA IEPEWLPVY VPSLCNLSE PLAEPEPRL KNGKVYCTV NGTFGSQGW TLPNVEIEY PRTINYYKW FGRFLLEGL VFKKLEKYK DVLLSQPAI MVKSWAKLQ PRTDAILKA LLSKEAVSK QRLAEIWTD TPKCGCRSR PMEK
- >TC015731 RA
atgaagaaa gacttcgtt tgcaaccag aaactcctt gtggaaaaa atgggttat ttcgctgaa gtgaccacg gggcaacgc ctcgaagac atggacatt tccgtccac tgtgacatt ggtattttc gaatggttg atgaaatgg gtgaagaaa gaatccttg cttgaggaa gactggccc caattggac ccccaatgc gtcatcccg atcctcgtt tctgcggcg tttcttcaa atggaaccg ctccaaaac gactgcctt ttgtactgc caccaacac atgaacgaa atcctccga acgtctacg aacctatcg tgtctcaac gactcgatt ttgacacgc cttgccgcc atgtacacc aatagcgaa gtggaggcg atcaaggac cgcaaagat aagatccaa tcacgcatt ttcacgaaa ttgatccaa tcgctggcg gagcctgag cctgaaagc atgcgtggc cattggtcg tcactggcg agggtgtat cgctgtggg cggtgccaa cagctgatt ggtccctcg gttgcgaca cgtatacct tgtgccccg ccttgtatg caattacag tgcgatggt tccataata agccaacac gtcagagac ccatcatgg aacataaat gactatatt ttaaaactg tacaagaat ttgaagact tggagaaaa gtttattgg agattatgg ggcgactcc cactttttg tattgtctg acgtatccg ccccaatat ttcaccctt gacgcccag aaagcgcca ctccccgtt gggcgttac ccctgttgt ggggagaga gcttatcgt ttccagtta ttacaagat tattccggg tgtcagttc cgggaacat acaattagc gcgaaagac gtgcgagat tcggccgtt ttgtcactt ttggagaat ttccggggg ttaatagag gaggagcct ccacagttg atgtttccg gaacgttta acccgattg gttgcacgt gacccggcc ggcgatagc aagaagttg gtttgtaaa gagaatttc tggtgggag gggtttcaa attgtcccg cccaggccc aagctggga ctgttgggc caatttgct agtcggggc ggggaagag gccgtgccg gaggaggag tcaagtgaa gggacggac gaaagtacg tccgacagt gatgatagt gacagtgag gggagcgac ggaagtgcc gaaagtaat cctccacca atgcctgtc aagaacctt aagaagaaa agaaataag cgggaaagt aggttgtgg caacataat ttgtcggcg agaagtaac caggatata caaagagcg tttgaggag aatgcggtt agggaaata acggccatg ttgaatcgg cggacggtc accatttcg gaaggcggg gccaaaagt gggcggttc tcgcggaat gtcacgccc attggtggg gtttgggtg cgattagag tccgaatgg aaggaagtc cattgcgct aataataac cagcaaaaa atgcgaatg cgattgttg gaaataaac aataaaatg gtcaaaaag cgctataat tggaagtcg cgacaggtt gttaacacc gttgttgat aattccgcg accaaaaag atccaactt gatttgcaa gtggagggc cactatgac caaagtaat gagttagtg ctgccttca aaaaaacgc aagacaaaa ataaaagat gagaaacaa aaaatcacc aaaatcctt tcaaagaaa cacagaaag aaacttgaa aaaatcgtc gagaaaaag aagaaaaag gagaaccgc gcgtcactc cttgaagct ttatcgaaa gtccaagca acccctgaa gagttaacc aaactaacc tcaattgcc actgtacaa acaaagggg ctaaaacgg cactatctc gaggagcaa accggagac aagaagaaa cctaaacgc gacttgcag ataatttcg ggcgaaatt aaactaaac agtttatcg ggggccaaa agacgccaa ttgctggaa caaacagtt gatactaaa ccaaatggg gaccccaac gtggtgggg ttcgagtcg tcagactca ggcaatgac tcggacccc gagcccacg aaaaatgaa ccaacagaa acagaacca gaaccaaaa gaaccacca attaaaaac gaaccaaag gaaacaaaa actaaaact ccaaccagg aaagttctt gcgccaaaa cctgcagtt ttcgtggac gtgatccgc gacgaagaa atccagaac agtcgcctc aagctcccg atcttggcc gaggagcag caaatcatg gaaacgatt aacgaaaat ccggttgtg ataatcgct ggtgagaca ggcagtggt aaaaccact caagtcccc cagtttttg tacgaagcg ggctatgcg ttgaaaaaa caaatcgcg gtgactgaa ccccgaaga gttgcagcg attgcaatg tcgaaacgt gtggcacaa gaaatgaat ttaagctcg aacgaagtg agctattta attcggttc gagggcaac gccaccgaa gacacaaaa attaagttt atgacagat ggcgtgtta ctaaaagag gtccaaaac gattttctc ttgtcacaa tattcagtt gttatcctt gatgaagcc cacgaaaga tcggtttat accgatatt ttaatcggg cttttatca cgcattgta cctttgcga gtcaaaagg ggggatccc ctaaaactc ataatcatg tcggctacg ttacgagtc gaagacttc acgaaaaat aagcgactt tttaagaaa actccccct gtgattaac gtggatagt cggcaattc cccgtcact gttcacttc aataaacgc acaaatgag gattatttg agtgagagt ttcactaaa gtggttaaa attcacacg aaactgccc gaaggcgga gttttggtt ttcgtgact gggcaacaa gaagtcaac tcacttgtg aaaaaactc cgcgctaaa ttccccttc aaagtcaag cttaatgaa gaggcggag cctacaaaa ctcaaacag aaaaaggtt tacgccccc gaggtgaat ctcgatgat tacgacccg gattggtcg agtgagagt gaggaggag gagtcggaa cgccccctg tatcacgcc ccgccgttg tgggtgtta cccctctat tcgatgctc cccacccac aagcaaaac cgagtgttt caagcgccg cccccagga tgtcgtttg tgcgtcgtt agtaccaac gtagcggag acaagtctc actatacct aatataaaa tacgtggtt gactcaggt cggactaaa gtgaaactg tacgataaa atcaccggt gtgagtagt tacgtggtc acgtggacc agcaaagct tcggccaat cagcgcgcc gggagggcg gggcgtacg gggccgggc cactgctac cgcctctat tccagtgct gtttttaac gacacgtta cacgatttt tgtgttccg gaaattcag caaaaacca gtcgatgat ttatacttg caaatgaaa tgtatgagt atcgataaa gtcgtaaac ttccccttc cccacagcc cccgactta ttgcaattg aaaacagcc gaacaccga ctggaaata ttgggggcg ttgcaaaac agccaagtc acgcctttg gggcgtgcc atcgccaaa ttccccgtg ttgccacgt ttcggcaaa atgttggcc ttgagtcac caacaagac ctcctcccg tacaccata tgtatggtg gcagcactg tcagtccag gaggtgttg ctagacgac gccaaatgg gcggcgctt cgtcgccag tgggcggcg acgggcaat tcgctccta ttgggcgac agtatggtg ctgctccgg gcggtgggc gcggccgaa ttcgccaac acccaaaac cgactggag caattctgt cacgccaat gggttgaga cacaaggcg gtgctcgaa attcgaaag ctgcgccta caattgacc aatgaaatc aaggctaat gtgccggag gccgatgtt gtggtagac cccgccctt gccccgccc actgacctt caagccaag cttctaaga cagatactt ctcgcaggg atgggcgac caaatcgcc aagaaaatc gaaagtgag gataagaaa tttaaatat ggataccag gccaacaat atggaggac ccagtcttt ttacattcg gggtctgta ttacgaaaa aatctgcca gagtttgtg atttatcac gaaatttac gaaactaat aaaatttat atgagaggg gtgacagcc attgaaccc gagtggttg ccggtttat gtccccagt ttgtgcaat ttgagtgaa cctttagcc gagcctgaa cctcgacta aaaaacgga aaagtgtat tgtacggta aatggaacg tttggtagt caaggctgg acgctaccg aacgttgaa atagaatat cccagaacc attaattat tataagtgg tttggtcga tttttattg gaaggtttg gtgtttaaa aaactggag aaatacaag gacgtgttg ttgagtcag ccggcgatt atggtcaaa agttgggcg aagttgcaa ccgcggacc gacgctatt ttgaaagcc ttgctgtcg aaggaggcg gtgtcgaag cagcgattg gcggagatt tggacggac acgcccaag tgtgggtgc cgcagtcgg cctatggag aagtga
- >TC015731 RA
acgaggtga aaaacatga agaaagact tcgtttgca accagaaac tccttgtgg aaaaaatgg gttatttcg ctgaagtga ccacggggc aacgcctcg aagacatgg acatttccg tccactgtg acattggta ttttcgaat ggttgatga aatgggtga agaaagaat ccttgcttg aggaagact ggccccaat tggaccccc aatgcgtca tcccgatcc tcgtttctg cggcgtttc ttcaaatgg aaccgctcc aaaacgact gccttttgt actgccacc aacacatga acgaaatcc tccgaacgt ctacgaacc tatcgtgtc tcaacgact cgattttga cacgccttg ccgccatgt acaccaata gcgaagtgg aggcgatca aggaccgca aagataaga tccaatcac gcattttca cgaaattga tccaatcgc tggcggagc ctgagcctg aaagcatgc gtggccatt ggtcgtcac tggcgaggg tgtatcgct gtgggcggt gccaacagc tgattggtc cctcggttg cgacacgta taccttgtg ccccgcctt gtatgcaat tacagtgcg atggttcca taataagcc aacacgtca gagacccat catggaaca taaatgact atattttaa aactgtaca agaatttga agacttgga gaaaagttt attggagat tatggggcg actcccact ttttgtatt gtctgacgt atccgcccc aatatttca cccttgacg cccagaaag cgccactcc ccgttgggc gttacccct gttgtgggg agagagctt atcgtttcc agttattac aagattatt ccgggtgtc agttccggg aacatacaa ttagcgcga aagacgtgc gagattcgg ccgttttgt cacttttgg agaatttcc gggggttaa tagaggagg agcctccac agttgatgt ttccggaac gtttaaccc gattggttg cacgtgacc cggccggcg atagcaaga agttggttt gtaaagaga atttctggt gggaggggt ttcaaattg tcccgccca ggcccaagc tgggactgt tgggccaat ttgctagtc ggggcgggg aagaggccg tgccggagg aggagtcaa gtgaaggga cggacgaaa gtacgtccg acagtgatg atagtgaca gtgagggga gcgacggaa gtgccgaaa gtaatcctc caccaatgc ctgtcaaga accttaaga agaaaagaa ataagcggg aaagtaggt tgtggcaac ataatttgt cggcgagaa gtaaccagg atatacaaa gagcgtttg aggagaatg cggttaggg aaataacgg ccatgttga atcggcgga cggtcacca tttcggaag gcggggcca aaagtgggc ggttctcgc ggaatgtca cgcccattg gtggggttt gggtgcgat tagagtccg aatggaagg aagtccatt gcgctaata ataaccagc aaaaaatgc gaatgcgat tgttggaaa taaacaata aaatggtca aaaagcgct ataattgga agtcgcgac aggttgtta acaccgttg ttgataatt ccgcgacca aaaagatcc aacttgatt tgcaagtgg agggccact atgaccaaa gtaatgagt tagtgctgc cttcaaaaa aacgcaaga caaaaataa aagatgaga aacaaaaaa tcaccaaaa tcctttcaa agaaacaca gaaagaaac ttgaaaaaa tcgtcgaga aaaagaaga aaaaggaga accgcgcgt cactccttg aagctttat cgaaagtcc aagcaaccc ctgaagagt taaccaaac taacctcaa ttgccactg tacaaacaa aggggctaa aacggcact atctcgagg agcaaaccg gagacaaga agaaaccta aacgcgact tgcagataa tttcgggcg aaattaaac taaacagtt tatcggggg ccaaaagac gccaattgc tggaacaaa cagttgata ctaaaccaa atggggacc ccaacgtgg tggggttcg agtcgtcag actcaggca atgactcgg accccgagc ccacgaaaa atgaaccaa cagaaacag aaccagaac caaaagaac caccaatta aaaacgaac caaaggaaa caaaaacta aaactccaa ccaggaaag ttcttgcgc caaaacctg cagttttcg tggacgtga tccgcgacg aagaaatcc agaacagtc gcctcaagc tcccgatct tggccgagg agcagcaaa tcatggaaa cgattaacg aaaatccgg ttgtgataa tcgctggtg agacaggca gtggtaaaa ccactcaag tcccccagt ttttgtacg aagcgggct atgcgttga aaaaacaaa tcgcggtga ctgaacccc gaagagttg cagcgattg caatgtcga aacgtgtgg cacaagaaa tgaatttaa gctcgaacg aagtgagct atttaattc ggttcgagg gcaacgcca ccgaagaca caaaaatta agtttatga cagatggcg tgttactaa aagaggtcc aaaacgatt ttctcttgt cacaatatt cagttgtta tccttgatg aagcccacg aaagatcgg tttataccg atattttaa tcgggcttt tatcacgca ttgtacctt tgcgagtca aaagggggg atcccctaa aactcataa tcatgtcgg ctacgttac gagtcgaag acttcacga aaaataagc gacttttta agaaaactc cccctgtga ttaacgtgg atagtcggc aattccccg tcactgttc acttcaata aacgcacaa atgaggatt atttgagtg agagtttca ctaaagtgg ttaaaattc acacgaaac tgcccgaag gcggagttt tggttttcg tgactgggc aacaagaag tcaactcac ttgtgaaaa aactccgcg ctaaattcc ccttcaaag tcaagctta atgaagagg cggagccta caaaactca aacagaaaa aggtttacg cccccgagg tgaatctcg atgattacg acccggatt ggtcgagtg agagtgagg aggaggagt cggaacgcc ccctgtatc acgccccgc cgttgtggg tgttacccc tctattcga tgctcccca cccacaagc aaaaccgag tgtttcaag cgccgcccc caggatgtc gtttgtgcg tcgttagta ccaacgtag cggagacaa gtctcacta tacctaata taaaatacg tggttgact caggtcgga ctaaagtga aactgtacg ataaaatca ccggtgtga gtagttacg tggtcacgt ggaccagca aagcttcgg ccaatcagc gcgccggga gggcggggc gtacggggc cgggccact gctaccgcc tctattcca gtgctgttt ttaacgaca cgttacacg atttttgtg ttccggaaa ttcagcaaa aaccagtcg atgatttat acttgcaaa tgaaatgta tgagtatcg ataaagtcg taaacttcc ccttcccca cagcccccg acttattgc aattgaaaa cagccgaac accgactgg aaatattgg gggcgttgc aaaacagcc aagtcacgc ctttggggc gtgccatcg ccaaattcc ccgtgttgc cacgtttcg gcaaaatgt tggccttga gtcaccaac aagacctcc tcccgtaca ccatatgta tggtggcag cactgtcag tccaggagg tgttgctag acgacgcca aatgggcgg cgcttcgtc gccagtggg cggcgacgg gcaattcgc tcctattgg gcgacagta tggtgctgc tccgggcgg tgggcgcgg ccgaattcg ccaacaccc aaaaccgac tggagcaat tctgtcacg ccaatgggt tgagacaca aggcggtgc tcgaaattc gaaagctgc gcctacaat tgaccaatg aaatcaagg ctaatgtgc cggaggccg atgttgtgg tagaccccg cccttgccc cgcccactg accttcaag ccaagcttc taagacaga tacttctcg cagggatgg gcgaccaaa tcgccaaga aaatcgaaa gtgaggata agaaattta aatatggat accaggcca acaatatgg aggacccag tctttttac attcggggt ctgtattac gaaaaaatc tgccagagt ttgtgattt atcacgaaa tttacgaaa ctaataaaa tttatatga gaggggtga cagccattg aacccgagt ggttgccgg tttatgtcc ccagtttgt gcaatttga gtgaacctt tagccgagc ctgaacctc gactaaaaa acggaaaag tgtattgta cggtaaatg gaacgtttg gtagtcaag gctggacgc taccgaacg ttgaaatag aatatccca gaaccatta attattata agtggtttg gtcgatttt tattggaag gtttggtgt ttaaaaaac tggagaaat acaaggacg tgttgttga gtcagccgg cgattatgg tcaaaagtt gggcgaagt tgcaaccgc ggaccgacg ctattttga aagccttgc tgtcgaagg aggcggtgt cgaagcagc gattggcgg agatttgga cggacacgc ccaagtgtg ggtgccgca gtcggccta tggagaagt gagcatgat gtggccgcc tttagatta at
