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Gene information
General information
TC033431
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014968 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5785194..5786345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033431
    iBeetle screen iB_08552

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult nutritional state - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033431 RA
      MSKQYGLIV PNKDKAVIK RPAIFDESD EEESVPTGA 
KKSLKKQDK ILQNRAVEE DPTVFQYDE IYDEMDKKR 
KESKLSRKD LDRKPKYIN KLLAAADRR KKENERRIE 
RQVQKERET EGDEFKDKE AFVTSSYKK KLEEMKEME 
ELERREEYL ESIGDVTKQ GNLDGFYRH LYDQKVNFE 
ETTKDKEQE EKTKDKKQE ETVEDKPSK RKYRKRHSS 
DSQSDKSEK EEETEVKKE HLPSNLDAD SDFSIDSSD 
SEEETEKKV TVEKVEEKK QEIKEEKEE EKQPEQAPE 
PPPKKPKID IWKKRTVGE VFDAALRRY FERKSLREL
    CDS sequence
    • >TC033431 RA
      atgtcaaaa cagtatggt ttaatcgtc ccaaacaag 
gacaaggca gtgattaaa cgccccgca attttcgat 
gagtccgat gaggaggag tcagtcccc acgggggcc 
aaaaaatcg cttaaaaaa caagacaaa attttgcaa 
aatcgggca gttgaggaa gacccaaca gtgttccag 
tatgacgag atttacgac gaaatggac aagaaacgt 
aaggagtca aaattgagt aggaaagat ctggacagg 
aagcccaaa tacataaat aaattgctt gctgctgct 
gatcgacgc aaaaaagag aatgaaagg cgcattgaa 
cgccaagta cagaaagaa agggagacg gaaggtgac 
gaatttaaa gataaggag gcgttcgtc actagtagt 
tacaagaag aaattggag gaaatgaaa gagatggag 
gaattggaa aggagggag gagtatttg gaatcgatt 
ggagacgtg acgaaacag gggaatttg gacgggttt 
taccgccat ttgtacgac cagaaagta aattttgaa 
gaaacaaca aaagataag gaacaagaa gaaaaaaca 
aaagataag aaacaagaa gaaacagta gaagataag 
ccaagtaag aggaaatac agaaaacgc cacagttca 
gactcacaa tcggataag agcgaaaag gaggaagaa 
acagaagtg aagaaagaa catctccct tcgaatcta 
gacgccgat tctgacttc agcatcgat tcttccgac 
agtgaggaa gaaactgag aaaaaagtg acggtggag 
aaagtggaa gagaagaaa caagaaatc aaggaggaa 
aaagaagaa gaaaaacaa ccggaacaa gcacctgaa 
cccccaccc aaaaaaccg aaaattgac atttggaaa 
aaaaggaca gttggggaa gtgtttgac gccgccttg 
cggaggtat tttgaaaga aagtcgtta agggaacta 
taa
    mRNA sequence
    • >TC033431 RA
      cgtaagagt cttagaaaa gtgtaaaaa ataattttc 
ttgagggtt aattgacag ttgtctaac ctcaaaacg 
tcaaactaa cgacaatgt caaaacagt atggtttaa 
tcgtcccaa acaaggaca aggcagtga ttaaacgcc 
ccgcaattt tcgatgagt ccgatgagg aggagtcag 
tccccacgg gggccaaaa aatcgctta aaaaacaag 
acaaaattt tgcaaaatc gggcagttg aggaagacc 
caacagtgt tccagtatg acgagattt acgacgaaa 
tggacaaga aacgtaagg agtcaaaat tgagtagga 
aagatctgg acaggaagc ccaaataca taaataaat 
tgcttgctg ctgctgatc gacgcaaaa aagagaatg 
aaaggcgca ttgaacgcc aagtacaga aagaaaggg 
agacggaag gtgacgaat ttaaagata aggaggcgt 
tcgtcacta gtagttaca agaagaaat tggaggaaa 
tgaaagaga tggaggaat tggaaagga gggaggagt 
atttggaat cgattggag acgtgacga aacagggga 
atttggacg ggttttacc gccatttgt acgaccaga 
aagtaaatt ttgaagaaa caacaaaag ataaggaac 
aagaagaaa aaacaaaag ataagaaac aagaagaaa 
cagtagaag ataagccaa gtaagagga aatacagaa 
aacgccaca gttcagact cacaatcgg ataagagcg 
aaaaggagg aagaaacag aagtgaaga aagaacatc 
tcccttcga atctagacg ccgattctg acttcagca 
tcgattctt ccgacagtg aggaagaaa ctgagaaaa 
aagtgacgg tggagaaag tggaagaga agaaacaag 
aaatcaagg aggaaaaag aagaagaaa aacaaccgg 
aacaagcac ctgaacccc cacccaaaa aaccgaaaa 
ttgacattt ggaaaaaaa ggacagttg gggaagtgt 
ttgacgccg ccttgcgga ggtattttg aaagaaagt 
cgttaaggg aactataat tttgagttt tgtaataaa 
taaaatttg gttcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY