Status
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Gene information
General information
TC015700 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5457373..5459314
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015700
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08550

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015700 RA
      MLKPVPFFF SLFALTSPQ SLLDGLINF IEEGDAQSF 
      NEPPDTPVL LPSYDFIIV GAGTAGCVL ANRLSENPS 
      WNVLLLEAG RPENYLMDL PVLANYIQF TDANWRYKT 
      EPSDKFCLG MENQQCNWP RGKVVGGSS VLNYMIYTR 
      GNWRDYDKW AELGNEGWG FKDVLPYFK KIENFMVPG 
      PYNASYHNH DGYLAVSYS PYKTKIADA VLESAQLMG 
      LKLVDYNGP IQVGVSRFQ VTLRDGIRE SSSRAYLHP 
      IKNRPNFHM RKYSTVTKI LIDPTTKKV QGVEVDTKG 
      TIYKIGASK EVLVAGGAV NSPQLLMLS GIGPKKHLT 
      QMGIPVLSN LKVGYNLLD HVALGGLTF RIDEPYSLK 
      TERVLSRES LFQFWNYHQ GPITAPGGC EVVVFHDLK 
      DPTNPDGYP DIELVFLGA SLSLDPLLQ KNLAISDYV 
      YKTVYTPIE RFDSFMVFP MILRPQSRG RIALRDNNY 
      KSKPRIFPN YFHVKEDME TIIGGVRLT LNITAQQPM 
      RKIGTRLHD IPIPQCAHL EFASDGYFE CMARHLTFT 
      IYHHCGTCK MGPRSDKSA VVDPRLRVY GVEGLRVID 
      ASVMPEVPA AHTNAPIFM IAEKGADMI KEEWVGNIG 
      AD
    CDS sequence
    • >TC015700 RA
      atgttaaaa ccagtcccg tttttcttt tccttgttt 
      gccctcaca agtccgcaa tctctcctc gatggcctt 
      atcaatttc atcgaagaa ggcgatgcc caaagcttc 
      aacgaaccc ccggacacc cctgtatta ctcccatct 
      tacgacttt atcatagta ggtgctgga acggctgga 
      tgtgtttta gccaatcgt ttgagcgaa aacccctcc 
      tggaatgta cttttgctg gaagcaggg cgtcccgaa 
      aactacctc atggacctt ccagtgttg gcaaactat 
      atccaattc accgacgcc aactggcgg tacaaaacc 
      gaaccaagc gacaagttt tgcctcgga atggaaaac 
      cagcaatgt aattggccg agaggtaag gtggtgggc 
      gggtcaagc gtcttgaat tacatgatc tacacgcgt 
      ggcaattgg cgggattat gacaagtgg gcggagctg 
      ggcaacgaa gggtggggc ttcaaggac gtgctaccc 
      tacttcaag aagattgaa aatttcatg gtaccagga 
      ccgtacaac gcgtcgtac cataaccat gatgggtat 
      ttggcggtc agctactcg ccctataaa accaaaatt 
      gccgatgct gtccttgaa tcggcccaa ctcatgggt 
      ttgaagctg gtcgattat aatgggccc atccaagtg 
      ggcgtgtcc cggtttcag gtgacttta agggacggg 
      attagggag agttcaagc cgggcttat ctacaccca 
      atcaaaaat cggcccaat tttcacatg agaaaatat 
      tcaactgtg acaaaaatt ttgattgac ccaacaacc 
      aaaaaagtc cagggtgtg gaggttgat accaaaggc 
      acgatttac aaaattggg gcttcgaag gaggttttg 
      gtggcggga ggggccgtc aactccccc caactcctc 
      atgttatca gggattggt cccaagaag cacttgacc 
      cagatgggc atccctgtc ttgtcaaat cttaaggtt 
      gggtacaac cttctggac catgtggcc ttgggcggg 
      ttgacgttc cgtatcgac gaaccttac tccctaaaa 
      accgaacgg gttctatcc cgggagagt ttgttccag 
      ttctggaac taccaccaa gggcccatc actgccccc 
      ggtgggtgc gaggttgtg gtcttccac gacttgaag 
      gacccaacg aacccagat gggtacccc gacattgag 
      cttgtcttc ctgggcgcg tccctaagt ctcgaccct 
      cttctccag aagaacttg gcgatttct gactacgtc 
      tacaaaacg gtgtacaca cccatcgaa cgcttcgat 
      tccttcatg gtcttcccc atgatcctc cggccccaa 
      agccggggc cgcatcgcc ctaagagac aacaactac 
      aagtcgaaa ccacgaatc ttccccaac tatttccac 
      gttaaggag gatatggag acgataatt ggaggcgtc 
      cggttaact ttaaacatc acggcccaa caacccatg 
      cgaaaaatt ggtacaaga ctccacgat atcccgata 
      ccacagtgt gcccatctg gagttcgcc tccgatggg 
      tattttgag tgcatggca aggcatctc acttttacc 
      atttaccat cactgtggg acttgcaag atgggtccg 
      aggagtgat aaatcggcg gtggttgat cccagattg 
      agggtttat ggggttgag ggtttgagg gtgattgat 
      gcgtcggtt atgccagaa gtgccagca gcacacacc 
      aatgcaccc atttttatg attgcggaa aaaggggcc 
      gatatgatc aaggaggag tgggttggc aacattgga 
      gccgattga
    mRNA sequence
    • >TC015700 RA
      catactata aaatgttaa aaccagtcc cgtttttct 
      tttccttgt ttgccctca caagtccgc aatctctcc 
      tcgatggcc ttatcaatt tcatcgaag aaggcgatg 
      cccaaagct tcaacgaac ccccggaca cccctgtat 
      tactcccat cttacgact ttatcatag taggtgctg 
      gaacggctg gatgtgttt tagccaatc gtttgagcg 
      aaaacccct cctggaatg tacttttgc tggaagcag 
      ggcgtcccg aaaactacc tcatggacc ttccagtgt 
      tggcaaact atatccaat tcaccgacg ccaactggc 
      ggtacaaaa ccgaaccaa gcgacaagt tttgcctcg 
      gaatggaaa accagcaat gtaattggc cgagaggta 
      aggtggtgg gcgggtcaa gcgtcttga attacatga 
      tctacacgc gtggcaatt ggcgggatt atgacaagt 
      gggcggagc tgggcaacg aagggtggg gcttcaagg 
      acgtgctac cctacttca agaagattg aaaatttca 
      tggtaccag gaccgtaca acgcgtcgt accataacc 
      atgatgggt atttggcgg tcagctact cgccctata 
      aaaccaaaa ttgccgatg ctgtccttg aatcggccc 
      aactcatgg gtttgaagc tggtcgatt ataatgggc 
      ccatccaag tgggcgtgt cccggtttc aggtgactt 
      taagggacg ggattaggg agagttcaa gccgggctt 
      atctacacc caatcaaaa atcggccca attttcaca 
      tgagaaaat attcaactg tgacaaaaa ttttgattg 
      acccaacaa ccaaaaaag tccagggtg tggaggttg 
      ataccaaag gcacgattt acaaaattg gggcttcga 
      aggaggttt tggtggcgg gaggggccg tcaactccc 
      cccaactcc tcatgttat cagggattg gtcccaaga 
      agcacttga cccagatgg gcatccctg tcttgtcaa 
      atcttaagg ttgggtaca accttctgg accatgtgg 
      ccttgggcg ggttgacgt tccgtatcg acgaacctt 
      actccctaa aaaccgaac gggttctat cccgggaga 
      gtttgttcc agttctgga actaccacc aagggccca 
      tcactgccc ccggtgggt gcgaggttg tggtcttcc 
      acgacttga aggacccaa cgaacccag atgggtacc 
      ccgacattg agcttgtct tcctgggcg cgtccctaa 
      gtctcgacc ctcttctcc agaagaact tggcgattt 
      ctgactacg tctacaaaa cggtgtaca cacccatcg 
      aacgcttcg attccttca tggtcttcc ccatgatcc 
      tccggcccc aaagccggg gccgcatcg ccctaagag 
      acaacaact acaagtcga aaccacgaa tcttcccca 
      actatttcc acgttaagg aggatatgg agacgataa 
      ttggaggcg tccggttaa ctttaaaca tcacggccc 
      aacaaccca tgcgaaaaa ttggtacaa gactccacg 
      atatcccga taccacagt gtgcccatc tggagttcg 
      cctccgatg ggtattttg agtgcatgg caaggcatc 
      tcactttta ccatttacc atcactgtg ggacttgca 
      agatgggtc cgaggagtg ataaatcgg cggtggttg 
      atcccagat tgagggttt atggggttg agggtttga 
      gggtgattg atgcgtcgg ttatgccag aagtgccag 
      cagcacaca ccaatgcac ccattttta tgattgcgg 
      aaaaagggg ccgatatga tcaaggagg agtgggttg 
      gcaacattg gagccgatt gagtgctaa taacgtgga 
      aaaataaat tatttaaaa aata
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