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Gene information
General information
TC015669 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5260198..5266279
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015669
    iBeetle screen iB_08549

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015669 RA
      MVQKRKVDA FFEAPNELV EFIKREIGE DDDRIKHVE 
      DLLQQRVHF DKHMPKNYD KAMARNFIR GAKFNLEIT 
      RRKLEGYFL AKKAFPMFY HNRNPNCKD LLRAFDTVN 
      IARMPKLTQ DGERVCILS LADSNPDNF IMLDVLKLF 
      WMLYDLTLT CDLPITSEI FIFDCASAK PEHLVKFLS 
      SSFKNSVSI LQDAYAARV SQLHVVNCP TMAEKVIAA 
      VRPLIHEKV KKRFIVHKG VESLLNHLQ PKVLPAEYG 
      GHLESIRDY SRSWQEILV ANADWFEGQ EGVTITGTP 
      PRGINKFDN GLEGSFRQL NID
    CDS sequence
    • >TC015669 RA
      atggtgcaa aagcggaaa gtggacgcc ttcttcgag 
      gcacccaac gaactcgtg gagttcatc aaacgcgag 
      atcggcgaa gatgacgac cggatcaaa cacgtggag 
      gatttactc cagcagagg gtgcacttc gacaaacac 
      atgcccaaa aactacgac aaagccatg gcgcgcaat 
      ttcatacgc ggggccaaa ttcaacctg gagatcacc 
      cgcaggaag ctggaggga tactttttg gccaagaag 
      gccttcccc atgttctac cacaacagg aaccccaac 
      tgcaaggac ttgctgcgg gccttcgat acggtgaac 
      atcgcacgt atgccgaaa ttgacgcaa gatggcgaa 
      cgcgtttgt attttgtca ctggctgat tcaaacccc 
      gataatttt ataatgttg gatgttttg aagctgttc 
      tggatgctg tacgacttg accctcacg tgcgacctt 
      cccatcacc agcgaaata ttcatcttc gactgtgcg 
      tccgccaaa cccgaacat ttggtgaaa ttcctcagc 
      agttcgttc aaaaattcg gtctcgatt cttcaagac 
      gcgtacgcg gcccgcgtt agtcaacta cacgtcgtc 
      aattgtccg acaatggcc gagaaagtg atcgccgcc 
      gttcggccc ctcatccac gaaaaagtc aaaaaaagg 
      tttattgtt cataaaggt gtggaatcg ctgcttaat 
      cacttgcaa ccgaaagtg ctaccggcc gaatacggg 
      ggacatctg gaaagtatc agggattat agtaggtca 
      tggcaggag atactcgtg gcgaatgcg gattggttt 
      gagggacaa gagggcgtg actatcaca ggaacgccc 
      ccacgcggg attaacaag tttgataat ggccttgag 
      ggtagtttt agacagttg aatatcgat tag
    mRNA sequence
    • >TC015669 RA
      aaccctata attatttgc acccaacct tgccgtaac 
      attttcccg attttttcc gacccaaaa aacgtttgc 
      accatttcc gcattcact cttaattga ctcaattaa 
      aaaccgatc aaatgttca accaaacga cagtcttaa 
      agagtcagg aaatccacc aaaacaccc gaaattcgc 
      acccgccct ctccgaaga aggccacca gatcaaagc 
      tctgcctta gccgtgaag agaaccggc gtggaggtc 
      ggccccatc cgcgcttat aaatagaag catgcgacc 
      tcttgcctg acttcagtc gacaatggt gcaaaagcg 
      gaaagtgga cgccttctt cgaggcacc caacgaact 
      cgtggagtt catcaaacg cgagatcgg cgaagatga 
      cgaccggat caaacacgt ggaggattt actccagca 
      gagggtgca cttcgacaa acacatgcc caaaaacta 
      cgacaaagc catggcgcg caatttcat acgcggggc 
      caaattcaa cctggagat cacccgcag gaagctgga 
      gggatactt tttggccaa gaaggcctt ccccatgtt 
      ctaccacaa caggaaccc caactgcaa ggacttgct 
      gcgggcctt cgatacggt gaacatcgc acgtatgcc 
      gaaattgac gcaagatgg cgaacgcgt ttgtatttt 
      gtcactggc tgattcaaa ccccgataa ttttataat 
      gttggatgt tttgaagct gttctggat gctgtacga 
      cttgaccct cacgtgcga ccttcccat caccagcga 
      aatattcat cttcgactg tgcgtccgc caaacccga 
      acatttggt gaaattcct cagcagttc gttcaaaaa 
      ttcggtctc gattcttca agacgcgta cgcggcccg 
      cgttagtca actacacgt cgtcaattg tccgacaat 
      ggccgagaa agtgatcgc cgccgttcg gcccctcat 
      ccacgaaaa agtcaaaaa aaggtttat tgttcataa 
      aggtgtgga atcgctgct taatcactt gcaaccgaa 
      agtgctacc ggccgaata cgggggaca tctggaaag 
      tatcaggga ttatagtag gtcatggca ggagatact 
      cgtggcgaa tgcggattg gtttgaggg acaagaggg 
      cgtgactat cacaggaac gcccccacg cgggattaa 
      caagtttga taatggcct tgagggtag ttttagaca 
      gttgaatat cgattagtt taaggctcg actgtgatt 
      atttcattt catttttat tttccacaa acaatacaa 
      aataactct taca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY