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Gene information
General information
TC015048 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4233759..4240212
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015048
    iBeetle screen iB_08541

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015048 RA
      MFDPNAITT QQSQIQYQQ PQPTDKDKT QETQKDTFP 
      SFCYANVNM IHKITPNTT QPHNSTVNM SQLSDDKCY 
      ITQPFSYNY ALVNQMSLT QNTISNITF KCDVCGLMF 
      AHLSLLNHH KRVHQTQGD QPQQQITVQ VQQPQQPTQ 
      IQIVSADRI RFPCEECGL TFNTQNDLK SHKIQTHQP 
      QPQPQNTLK IKNCESCGA PLPQDNNKK RAVMKVKCE 
      NCLSAEAIQ PQIFVVATA SDSNVKFEE STGTQTTNQ 
      SNLGTQNSI PIGVKNNHP VKRRGVASV TKCTKCNGS 
      GIIFIGGSK NQQNNVDKP FHCNICNGS FSRYSSLWS 
      HKRLHTGEK NFKCNICGL AFAKAAYLK NHSRIHTGE 
      KPYRCNVCG MQFSQSPHL KNHERIHSG ERPYVCEVC 
      EKSFARHST LWNHRRIHT GEKPYRCDI CGSCFNQAT 
      HLKNHAKVH SGEKPFKCD ICSVGFSDR FALKRHRNI 
      HEKYGRTKP NPPPASTTD DTQTNNTTT DSSNTTTVT 
      TVATATQDA NEHEELLET KYEEKFVPS DEITEEMTD 
      ISELQVQLS
    CDS sequence
    • >TC015048 RA
      atgttcgac cccaacgcc atcaccacc cagcagtcg 
      caaatccaa taccaacag ccccaaccc accgacaag 
      gacaagacc caggagacc caaaaggac actttcccg 
      tcattttgc tacgccaac gtcaacatg atccacaaa 
      atcacccca aacaccacc caaccgcac aactccacc 
      gtcaacatg tcccaactt tccgacgac aaatgctac 
      atcacccaa cccttctcc tacaactac gccctggtc 
      aaccaaatg tccctcact cagaacacc atctcgaat 
      ataacgttc aaatgcgac gtctgcggc ctgatgttc 
      gcgcacttg tcgctcctc aaccaccac aagcgcgtg 
      caccagacc cagggcgac caaccccag cagcagatc 
      accgtccag gtccagcaa ccccaacag ccaacccag 
      atccagatc gtgtcggcc gatcgcatc cgatttcca 
      tgcgaggag tgcggactc actttcaac actcaaaac 
      gatctcaaa tcacacaaa atccagacg catcagccg 
      cagccccag cctcaaaac accctcaaa attaaaaat 
      tgcgaatcg tgcggagcc cccctacca caagacaat 
      aacaaaaaa cgagctgtt atgaaagtt aaatgcgag 
      aattgtctc tcggccgaa gcaatacag ccccagatt 
      ttcgtcgtc gcgacagcg agtgactca aacgtcaag 
      tttgaggag tcgacaggg acacaaaca accaatcag 
      agtaatcta ggcacgcaa aactcgatt cccattggt 
      gttaaaaat aatcatccg gtgaagagg cggggcgtt 
      gcgagtgtc acaaaatgt accaaatgt aacggaagt 
      ggtattatt ttcatcggc gggtccaaa aaccaacaa 
      aacaatgtc gataaaccg tttcattgc aacatctgc 
      aatggctcc ttcagtcgt tactcttcc ctttggtcg 
      cacaaacgc ctccacacc ggcgaaaaa aacttcaaa 
      tgtaacatt tgtggtttg gcgttcgcc aaagccgcc 
      tatctcaaa aaccattca cgcatccat accggggaa 
      aaaccgtac cggtgcaat gtttgcggt atgcaattt 
      tcacaatcc ccgcatttg aaaaaccac gaacggata 
      cattcgggg gaacgaccg tatgtgtgt gaagtgtgc 
      gaaaaatcg ttcgcacgt cattctacg ttatggaac 
      catcggagg attcacacg ggggaaaaa ccctaccga 
      tgcgacatc tgtggctcg tgtttcaac caagccacg 
      catttgaaa aaccacgcc aaggtgcat tcgggcgaa 
      aagccgttc aagtgtgat atttgctct gtggggttc 
      tcagacaga tttgcgctc aagcggcat aggaacatc 
      catgagaaa tacggcagg accaagcct aacccccct 
      ccagcttcc acaaccgat gatactcag acaaataat 
      acgacaact gactcttcg aatactacg actgttacg 
      acagtcgca acagcgacg caagacgca aacgaacac 
      gaagaactt ttagaaacg aaatatgag gaaaaattc 
      gtgccctct gatgaaatc actgaagaa atgaccgac 
      atctccgaa ctacaagtc caattatcc taa
    mRNA sequence
    • >TC015048 RA
      tcgcgtccg agcgagggg aacaaagat ggctatggc 
      tctatccgc cattttcta aagtgagat agactaaag 
      tgtttttcg tgaaaatag catgtttga ggcggaaaa 
      gcgcaatat ttccgtgga aaagtgtta aaatccggt 
      cgaagaaca tagttttgg ggctgtggc gatgcgtcg 
      cattgaaaa ttagtgttt tttccggtg ttgaaaatc 
      ggcgtctct gtggaaaca gcgccgtgt gagagtaaa 
      gttacgtca ccactcaac cgtcacaat gttcgaccc 
      caacgccat caccaccca gcagtcgca aatccaata 
      ccaacagcc ccaacccac cgacaagga caagaccca 
      ggagaccca aaaggacac tttcccgtc attttgcta 
      cgccaacgt caacatgat ccacaaaat caccccaaa 
      caccaccca accgcacaa ctccaccgt caacatgtc 
      ccaactttc cgacgacaa atgctacat cacccaacc 
      cttctccta caactacgc cctggtcaa ccaaatgtc 
      cctcactca gaacaccat ctcgaatat aacgttcaa 
      atgcgacgt ctgcggcct gatgttcgc gcacttgtc 
      gctcctcaa ccaccacaa gcgcgtgca ccagaccca 
      gggcgacca accccagca gcagatcac cgtccaggt 
      ccagcaacc ccaacagcc aacccagat ccagatcgt 
      gtcggccga tcgcatccg atttccatg cgaggagtg 
      cggactcac tttcaacac tcaaaacga tctcaaatc 
      acacaaaat ccagacgca tcagccgca gccccagcc 
      tcaaaacac cctcaaaat taaaaattg cgaatcgtg 
      cggagcccc cctaccaca agacaataa caaaaaacg 
      agctgttat gaaagttaa atgcgagaa ttgtctctc 
      ggccgaagc aatacagcc ccagatttt cgtcgtcgc 
      gacagcgag tgactcaaa cgtcaagtt tgaggagtc 
      gacagggac acaaacaac caatcagag taatctagg 
      cacgcaaaa ctcgattcc cattggtgt taaaaataa 
      tcatccggt gaagaggcg gggcgttgc gagtgtcac 
      aaaatgtac caaatgtaa cggaagtgg tattatttt 
      catcggcgg gtccaaaaa ccaacaaaa caatgtcga 
      taaaccgtt tcattgcaa catctgcaa tggctcctt 
      cagtcgtta ctcttccct ttggtcgca caaacgcct 
      ccacaccgg cgaaaaaaa cttcaaatg taacatttg 
      tggtttggc gttcgccaa agccgccta tctcaaaaa 
      ccattcacg catccatac cggggaaaa accgtaccg 
      gtgcaatgt ttgcggtat gcaattttc acaatcccc 
      gcatttgaa aaaccacga acggataca ttcggggga 
      acgaccgta tgtgtgtga agtgtgcga aaaatcgtt 
      cgcacgtca ttctacgtt atggaacca tcggaggat 
      tcacacggg ggaaaaacc ctaccgatg cgacatctg 
      tggctcgtg tttcaacca agccacgca tttgaaaaa 
      ccacgccaa ggtgcattc gggcgaaaa gccgttcaa 
      gtgtgatat ttgctctgt ggggttctc agacagatt 
      tgcgctcaa gcggcatag gaacatcca tgagaaata 
      cggcaggac caagcctaa cccccctcc agcttccac 
      aaccgatga tactcagac aaataatac gacaactga 
      ctcttcgaa tactacgac tgttacgac agtcgcaac 
      agcgacgca agacgcaaa cgaacacga agaactttt 
      agaaacgaa atatgagga aaaattcgt gccctctga 
      tgaaatcac tgaagaaat gaccgacat ctccgaact 
      acaagtcca attatccta aatgattaa gtattatta 
      cttggcttt ttgtatata ttcttccgt ttagtatag 
      agtttacgc aattaattt ttatttgat atttttatg 
      acatcagct tcgttatca tgaaatagg atgctatag 
      gtaggacta gaataacaa gagaaaaat aattttatt 
      gccattttg ttgttacga tgatttttt gactgcgct 
      ttgccctcc attatttgt tagtggtta ctttacagt 
      aataaatgc tcaaagtat ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY