| General information | |||
| TC015635 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 3775796..3783085 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC015635 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08537 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015635 RA
MDSETESQP LMSSCSSQS FGSTNECGF KNDSDTDTE ADRATKIQA ATIPPILIQ KQNRQFLYK SSYEHPDFE VYSNDGDDY KTDILTGEK SLSGKNGAP VNYDYRDPE LPETNSSLV TIFAIWNTT VGTSLLSMS WGIEKTGLF PAIFINIAI AAICLYTTY TILKVNEKH GVIGQNGEV CDLCRALIG RWAEILAKV FSLVVLIGA NIVYWILMS NFFYHSVQF FYGILLHLD DTPVSNVTV LCPKNETIV QIATSTPTN QFARIWDLY STVPVILGL IMFPMLNWK SPTFFTKFN SLGTFLPVW SGSIAKRLV LGTFSVLYL IVFVATKAG IWGINMPDW EIEFYMKPT FCALSGMLS LSYFIHNII ISIMRNNKH QEHNGRDLT IAFGLVTFT YLFIGVVFY VCFPLPKFC IEDNILNNF NKYDVLTII ARILLLFQL FTVFPLISY MLRNDVFAN INMIFKNYR FGEFTYPRV MTLNLAILA ICILFACFL PRIGTLIRY TGALSGLVY IFMLPSLLK IASLKKEGR LTTPKLIFH AAVMILGSF NLLLQFFIS DK
- >TC015635 RB
MDSETESQP LMSSCSSQS FGSTNECGF KNDSDTDTE ADRATKIQA ATIPPILIQ KQNRQFLYK SSYEHPDFE VYSNDGDDY KTDILTGEK SLSGKNGAP VNYDYRDPE LPETNSSLV TIFAIWNTT VGTSLLSMS WGIEKTGLF PAIFINIAI AAICLYTTY TILKVNEKH GVIGQNGEV CDLCRALIG RWAEILAKV FSLVVLIGA NIVYWILMS NFFYHSVQF FYGILLHLD DTPVSNVTV LCPKNETIV QIATSTPTN QFARIWDLY STVPVILGL IMFPMLNWK SPTFFTKFN SLGTFLPVW SGSIAKRLV LGTFSVLYL IVFVATKAG IWGINMPDW EIEFYMKPT FCALSGMLS LSYFIHNII ISIMRNNKH QEHNGRDLT IAFGLVTFT YLFIGVVFY VCFPLPKFC IEDQKMDEE NNGQIANYL LVCPRDCED HTSVLQLFR RLKPPNCPF IATRVWSRD TRKQKCCMW GVLYQSIDE TPYLQGTQA KELRKQLNA WRPIGGEIV DYEFYQNQA TNESGWVFT WRRLACTHQ PHFPKQDNT RTEELKMYM
- >TC015635 RC
MDEENNGQI ANYLLVCPR DCEDHTSVL QLFRRLKPP NCPFIATRV WSRDTRKQK CCMWGVLYQ SIDETPYLQ GTQAKELRK QLNAWRPIG GEIVDYEFY QNQATNESG WVFTWRRLA CTHQPHFPK QDNTRTEEL KMYM
- >TC015635 RA
atggactct gaaaccgaa tcccagcct ttgatgagc tcgtgttca agtcaaagt ttcggaagt actaacgaa tgtggcttt aaaaatgac tctgacact gatactgag gctgataga gccacgaaa atacaagca gccacaatt ccacctatt ctgatccaa aaacaaaac agacaattt ttgtataag tcgagctac gaacatcca gattttgag gtgtattcg aacgatggt gacgactac aagaccgat attttaacc ggggagaag tctctaagc ggcaaaaac ggcgctccg gtcaactac gactaccgc gacccggaa ttaccggaa accaacagt tctctcgtc actatattc gcaatttgg aacaccaca gtgggcacc tctctcttg tcaatgtcc tggggcatt gaaaaaacc ggacttttt cccgcaatt ttcataaac atcgccatt gccgccatt tgcttgtac acaacctat actatactg aaagttaac gaaaagcat ggcgtgatt ggccaaaat ggcgaagtt tgtgacttg tgtcgcgct ctgattggc cgatgggcg gagatttta gccaaggtg ttctcgctc gtggttctg attggggca aatattgtt tattggatt ttaatgtcg aattttttc tatcactcc gtccaattt ttttacgga attttgcta catttggac gacactccc gtctcgaat gtcacagtt ttgtgtccc aaaaacgaa acaatcgtc caaatcgcc accagtacg ccgaccaac caattcgcc cgaatctgg gacttgtat tcgaccgta cctgtgata ttaggcttg attatgttt ccgatgttg aactggaag agcccaact tttttcaca aaattcaat agtttaggt acgtttttg cctgtttgg agtggcagc attgccaaa aggctggtt ttagggact ttcagcgtt ttgtattta attgttttc gttgcaacc aaagcgggg atttggggg attaacatg ccagactgg gagattgaa ttttacatg aaaccgact ttctgtgcc ctcagcgga atgttatct ttgagttat ttcattcac aacattatc atttcgatt atgaggaac aacaaacac caagaacac aacggacgt gatttgaca attgcgttc ggtttggtg actttcact tatttattt atcggagtt gttttctat gtttgtttt ccgttaccg aagttctgt atagaggat aatatctta aataatttt aacaaatac gacgttttg acaattata gctaggatt ttgttactg tttcaactg tttactgtg tttcccctg atttcgtac atgttgcgt aacgacgtg tttgccaac attaacatg attttcaaa aattaccga tttggggaa tttacctat ccgagggtt atgacactt aatttggcc attttggca atttgtatt ttatttgcg tgttttctt ccaagaata gggacttta atcagatat acaggggct ttaagtggt ttagtctac atttttatg ttgccaagt ttgttaaaa atcgccagt ttgaagaag gaaggaaga ctgacgact ccgaaacta atcttccat gccgctgtt atgattctc ggcagtttt aatcttctc ttacaattc tttataagt gataaataa
- >TC015635 RB
atggactct gaaaccgaa tcccagcct ttgatgagc tcgtgttca agtcaaagt ttcggaagt actaacgaa tgtggcttt aaaaatgac tctgacact gatactgag gctgataga gccacgaaa atacaagca gccacaatt ccacctatt ctgatccaa aaacaaaac agacaattt ttgtataag tcgagctac gaacatcca gattttgag gtgtattcg aacgatggt gacgactac aagaccgat attttaacc ggggagaag tctctaagc ggcaaaaac ggcgctccg gtcaactac gactaccgc gacccggaa ttaccggaa accaacagt tctctcgtc actatattc gcaatttgg aacaccaca gtgggcacc tctctcttg tcaatgtcc tggggcatt gaaaaaacc ggacttttt cccgcaatt ttcataaac atcgccatt gccgccatt tgcttgtac acaacctat actatactg aaagttaac gaaaagcat ggcgtgatt ggccaaaat ggcgaagtt tgtgacttg tgtcgcgct ctgattggc cgatgggcg gagatttta gccaaggtg ttctcgctc gtggttctg attggggca aatattgtt tattggatt ttaatgtcg aattttttc tatcactcc gtccaattt ttttacgga attttgcta catttggac gacactccc gtctcgaat gtcacagtt ttgtgtccc aaaaacgaa acaatcgtc caaatcgcc accagtacg ccgaccaac caattcgcc cgaatctgg gacttgtat tcgaccgta cctgtgata ttaggcttg attatgttt ccgatgttg aactggaag agcccaact tttttcaca aaattcaat agtttaggt acgtttttg cctgtttgg agtggcagc attgccaaa aggctggtt ttagggact ttcagcgtt ttgtattta attgttttc gttgcaacc aaagcgggg atttggggg attaacatg ccagactgg gagattgaa ttttacatg aaaccgact ttctgtgcc ctcagcgga atgttatct ttgagttat ttcattcac aacattatc atttcgatt atgaggaac aacaaacac caagaacac aacggacgt gatttgaca attgcgttc ggtttggtg actttcact tatttattt atcggagtt gttttctat gtttgtttt ccgttaccg aagttctgt atagaggat cagaagatg gatgaggag aacaatggt caaattgct aattatctc ttggtgtgc ccaagagat tgcgaagat cataccagt gtccttcaa ctgttccgc agactcaag ccacctaat tgccctttt atagcgacc agggtctgg agtcgggac acccgaaaa caaaaatgc tgtatgtgg ggtgtgtta tatcaaagt attgatgaa acaccatat ttacaaggc acacaagct aaagaacta agaaaacag ttgaatgca tggagacca ataggtgga gaaattgta gattatgaa ttctaccaa aatcaagcc accaatgaa agtgggtgg gtgttcaca tggaggagg ttggcgtgt acccatcaa ccccatttt cctaagcaa gacaatact cgaacagag gagctcaaa atgtacatg tag
- >TC015635 RC
atggatgag gagaacaat ggtcaaatt gctaattat ctcttggtg tgcccaaga gattgcgaa gatcatacc agtgtcctt caactgttc cgcagactc aagccacct aattgccct tttatagcg accagggtc tggagtcgg gacacccga aaacaaaaa tgctgtatg tggggtgtg ttatatcaa agtattgat gaaacacca tatttacaa ggcacacaa gctaaagaa ctaagaaaa cagttgaat gcatggaga ccaataggt ggagaaatt gtagattat gaattctac caaaatcaa gccaccaat gaaagtggg tgggtgttc acatggagg aggttggcg tgtacccat caaccccat tttcctaag caagacaat actcgaaca gaggagctc aaaatgtac atgtag
- >TC015635 RA
gataaatct gaaaaaaaa aatcacaaa ataacaaac cttctctta tcaaagttt gttcttatc acgatgcat attaaacga agccagaaa gtggtcagt gttagtggc acgcagaac gttcttcgt tatctcacg aattttcga agataagtc gtcgtttcg aaacaatcg accaaacac ctcttgtgt aattatgaa tggaacttt tacttgcaa aattgttag tgcggtttt tgtaaaaca gttcgtcaa gattttcat gttgtttta tgttgtttt attctgacg tcatgatga gagatttaa tccgaaatt acaaactat tactccaac gaaaatccg acatggact ctgaaaccg aatcccagc ctttgatga gctcgtgtt caagtcaaa gtttcggaa gtactaacg aatgtggct ttaaaaatg actctgaca ctgatactg aggctgata gagccacga aaatacaag cagccacaa ttccaccta ttctgatcc aaaaacaaa acagacaat ttttgtata agtcgagct acgaacatc cagattttg aggtgtatt cgaacgatg gtgacgact acaagaccg atattttaa ccggggaga agtctctaa gcggcaaaa acggcgctc cggtcaact acgactacc gcgacccgg aattaccgg aaaccaaca gttctctcg tcactatat tcgcaattt ggaacacca cagtgggca cctctctct tgtcaatgt cctggggca ttgaaaaaa ccggacttt ttcccgcaa ttttcataa acatcgcca ttgccgcca tttgcttgt acacaacct atactatac tgaaagtta acgaaaagc atggcgtga ttggccaaa atggcgaag tttgtgact tgtgtcgcg ctctgattg gccgatggg cggagattt tagccaagg tgttctcgc tcgtggttc tgattgggg caaatattg tttattgga ttttaatgt cgaattttt tctatcact ccgtccaat ttttttacg gaattttgc tacatttgg acgacactc ccgtctcga atgtcacag ttttgtgtc ccaaaaacg aaacaatcg tccaaatcg ccaccagta cgccgacca accaattcg cccgaatct gggacttgt attcgaccg tacctgtga tattaggct tgattatgt ttccgatgt tgaactgga agagcccaa cttttttca caaaattca atagtttag gtacgtttt tgcctgttt ggagtggca gcattgcca aaaggctgg ttttaggga ctttcagcg ttttgtatt taattgttt tcgttgcaa ccaaagcgg ggatttggg ggattaaca tgccagact gggagattg aattttaca tgaaaccga ctttctgtg ccctcagcg gaatgttat ctttgagtt atttcattc acaacatta tcatttcga ttatgagga acaacaaac accaagaac acaacggac gtgatttga caattgcgt tcggtttgg tgactttca cttatttat ttatcggag ttgttttct atgtttgtt ttccgttac cgaagttct gtatagagg ataatatct taaataatt ttaacaaat acgacgttt tgacaatta tagctagga ttttgttac tgtttcaac tgtttactg tgtttcccc tgatttcgt acatgttgc gtaacgacg tgtttgcca acattaaca tgattttca aaaattacc gatttgggg aatttacct atccgaggg ttatgacac ttaatttgg ccattttgg caatttgta ttttatttg cgtgttttc ttccaagaa tagggactt taatcagat atacagggg ctttaagtg gtttagtct acattttta tgttgccaa gtttgttaa aaatcgcca gtttgaaga aggaaggaa gactgacga ctccgaaac taatcttcc atgccgctg ttatgattc tcggcagtt ttaatcttc tcttacaat tctttataa gtgataaat aatttttta ttgttgtta tgactgatt ctaataaaa tatttttac aaaa
- >TC015635 RB
gataaatct gaaaaaaaa aatcacaaa ataacaaac cttctctta tcaaagttt gttcttatc acgatgcat attaaacga agccagaaa gtggtcagt gttagtggc acgcagaac gttcttcgt tatctcacg aattttcga agataagtc gtcgtttcg aaacaatcg accaaacac ctcttgtgt aattatgaa tggaacttt tacttgcaa aattgttag tgcggtttt tgtaaaaca gttcgtcaa gattttcat gttgtttta tgttgtttt attctgacg tcatgatga gagatttaa tccgaaatt acaaactat tactccaac gaaaatccg acatggact ctgaaaccg aatcccagc ctttgatga gctcgtgtt caagtcaaa gtttcggaa gtactaacg aatgtggct ttaaaaatg actctgaca ctgatactg aggctgata gagccacga aaatacaag cagccacaa ttccaccta ttctgatcc aaaaacaaa acagacaat ttttgtata agtcgagct acgaacatc cagattttg aggtgtatt cgaacgatg gtgacgact acaagaccg atattttaa ccggggaga agtctctaa gcggcaaaa acggcgctc cggtcaact acgactacc gcgacccgg aattaccgg aaaccaaca gttctctcg tcactatat tcgcaattt ggaacacca cagtgggca cctctctct tgtcaatgt cctggggca ttgaaaaaa ccggacttt ttcccgcaa ttttcataa acatcgcca ttgccgcca tttgcttgt acacaacct atactatac tgaaagtta acgaaaagc atggcgtga ttggccaaa atggcgaag tttgtgact tgtgtcgcg ctctgattg gccgatggg cggagattt tagccaagg tgttctcgc tcgtggttc tgattgggg caaatattg tttattgga ttttaatgt cgaattttt tctatcact ccgtccaat ttttttacg gaattttgc tacatttgg acgacactc ccgtctcga atgtcacag ttttgtgtc ccaaaaacg aaacaatcg tccaaatcg ccaccagta cgccgacca accaattcg cccgaatct gggacttgt attcgaccg tacctgtga tattaggct tgattatgt ttccgatgt tgaactgga agagcccaa cttttttca caaaattca atagtttag gtacgtttt tgcctgttt ggagtggca gcattgcca aaaggctgg ttttaggga ctttcagcg ttttgtatt taattgttt tcgttgcaa ccaaagcgg ggatttggg ggattaaca tgccagact gggagattg aattttaca tgaaaccga ctttctgtg ccctcagcg gaatgttat ctttgagtt atttcattc acaacatta tcatttcga ttatgagga acaacaaac accaagaac acaacggac gtgatttga caattgcgt tcggtttgg tgactttca cttatttat ttatcggag ttgttttct atgtttgtt ttccgttac cgaagttct gtatagagg atcagaaga tggatgagg agaacaatg gtcaaattg ctaattatc tcttggtgt gcccaagag attgcgaag atcatacca gtgtccttc aactgttcc gcagactca agccaccta attgccctt ttatagcga ccagggtct ggagtcggg acacccgaa aacaaaaat gctgtatgt ggggtgtgt tatatcaaa gtattgatg aaacaccat atttacaag gcacacaag ctaaagaac taagaaaac agttgaatg catggagac caataggtg gagaaattg tagattatg aattctacc aaaatcaag ccaccaatg aaagtgggt gggtgttca catggagga ggttggcgt gtacccatc aaccccatt ttcctaagc aagacaata ctcgaacag aggagctca aaatgtaca tgtagtgat agtaaataa ttatggtat tttttaata aaagtgtgg aatccctta attagtatt tatgatgta cctactcac acataaata aagaaatca agactta
- >TC015635 RC
acttcctgc caatgctcg ttcagttgc ttcagtcct gtgcaagtc ctgtggttc agtctatgc atagatcct ccattatct ataattcta tgctgtgat tctatctta tcagtcaac cagaagatg gatgaggag aacaatggt caaattgct aattatctc ttggtgtgc ccaagagat tgcgaagat cataccagt gtccttcaa ctgttccgc agactcaag ccacctaat tgccctttt atagcgacc agggtctgg agtcgggac acccgaaaa caaaaatgc tgtatgtgg ggtgtgtta tatcaaagt attgatgaa acaccatat ttacaaggc acacaagct aaagaacta agaaaacag ttgaatgca tggagacca ataggtgga gaaattgta gattatgaa ttctaccaa aatcaagcc accaatgaa agtgggtgg gtgttcaca tggaggagg ttggcgtgt acccatcaa ccccatttt cctaagcaa gacaatact cgaacagag gagctcaaa atgtacatg tagtgatag taaataatt atggtattt tttaataaa agtgtggaa tcccttaat tagtattta tgatgtacc tactcacac ataaataaa gaaatcaag actta
