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Gene information
General information
TC015635 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3775796..3783085
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015635
    iBeetle screen iB_08537

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015635 RA
      MDSETESQP LMSSCSSQS FGSTNECGF KNDSDTDTE 
      ADRATKIQA ATIPPILIQ KQNRQFLYK SSYEHPDFE 
      VYSNDGDDY KTDILTGEK SLSGKNGAP VNYDYRDPE 
      LPETNSSLV TIFAIWNTT VGTSLLSMS WGIEKTGLF 
      PAIFINIAI AAICLYTTY TILKVNEKH GVIGQNGEV 
      CDLCRALIG RWAEILAKV FSLVVLIGA NIVYWILMS 
      NFFYHSVQF FYGILLHLD DTPVSNVTV LCPKNETIV 
      QIATSTPTN QFARIWDLY STVPVILGL IMFPMLNWK 
      SPTFFTKFN SLGTFLPVW SGSIAKRLV LGTFSVLYL 
      IVFVATKAG IWGINMPDW EIEFYMKPT FCALSGMLS 
      LSYFIHNII ISIMRNNKH QEHNGRDLT IAFGLVTFT 
      YLFIGVVFY VCFPLPKFC IEDNILNNF NKYDVLTII 
      ARILLLFQL FTVFPLISY MLRNDVFAN INMIFKNYR 
      FGEFTYPRV MTLNLAILA ICILFACFL PRIGTLIRY 
      TGALSGLVY IFMLPSLLK IASLKKEGR LTTPKLIFH 
      AAVMILGSF NLLLQFFIS DK
    • >TC015635 RB
      MDSETESQP LMSSCSSQS FGSTNECGF KNDSDTDTE 
      ADRATKIQA ATIPPILIQ KQNRQFLYK SSYEHPDFE 
      VYSNDGDDY KTDILTGEK SLSGKNGAP VNYDYRDPE 
      LPETNSSLV TIFAIWNTT VGTSLLSMS WGIEKTGLF 
      PAIFINIAI AAICLYTTY TILKVNEKH GVIGQNGEV 
      CDLCRALIG RWAEILAKV FSLVVLIGA NIVYWILMS 
      NFFYHSVQF FYGILLHLD DTPVSNVTV LCPKNETIV 
      QIATSTPTN QFARIWDLY STVPVILGL IMFPMLNWK 
      SPTFFTKFN SLGTFLPVW SGSIAKRLV LGTFSVLYL 
      IVFVATKAG IWGINMPDW EIEFYMKPT FCALSGMLS 
      LSYFIHNII ISIMRNNKH QEHNGRDLT IAFGLVTFT 
      YLFIGVVFY VCFPLPKFC IEDQKMDEE NNGQIANYL 
      LVCPRDCED HTSVLQLFR RLKPPNCPF IATRVWSRD 
      TRKQKCCMW GVLYQSIDE TPYLQGTQA KELRKQLNA 
      WRPIGGEIV DYEFYQNQA TNESGWVFT WRRLACTHQ 
      PHFPKQDNT RTEELKMYM
    • >TC015635 RC
      MDEENNGQI ANYLLVCPR DCEDHTSVL QLFRRLKPP 
      NCPFIATRV WSRDTRKQK CCMWGVLYQ SIDETPYLQ 
      GTQAKELRK QLNAWRPIG GEIVDYEFY QNQATNESG 
      WVFTWRRLA CTHQPHFPK QDNTRTEEL KMYM
    CDS sequence
    • >TC015635 RA
      atggactct gaaaccgaa tcccagcct ttgatgagc 
      tcgtgttca agtcaaagt ttcggaagt actaacgaa 
      tgtggcttt aaaaatgac tctgacact gatactgag 
      gctgataga gccacgaaa atacaagca gccacaatt 
      ccacctatt ctgatccaa aaacaaaac agacaattt 
      ttgtataag tcgagctac gaacatcca gattttgag 
      gtgtattcg aacgatggt gacgactac aagaccgat 
      attttaacc ggggagaag tctctaagc ggcaaaaac 
      ggcgctccg gtcaactac gactaccgc gacccggaa 
      ttaccggaa accaacagt tctctcgtc actatattc 
      gcaatttgg aacaccaca gtgggcacc tctctcttg 
      tcaatgtcc tggggcatt gaaaaaacc ggacttttt 
      cccgcaatt ttcataaac atcgccatt gccgccatt 
      tgcttgtac acaacctat actatactg aaagttaac 
      gaaaagcat ggcgtgatt ggccaaaat ggcgaagtt 
      tgtgacttg tgtcgcgct ctgattggc cgatgggcg 
      gagatttta gccaaggtg ttctcgctc gtggttctg 
      attggggca aatattgtt tattggatt ttaatgtcg 
      aattttttc tatcactcc gtccaattt ttttacgga 
      attttgcta catttggac gacactccc gtctcgaat 
      gtcacagtt ttgtgtccc aaaaacgaa acaatcgtc 
      caaatcgcc accagtacg ccgaccaac caattcgcc 
      cgaatctgg gacttgtat tcgaccgta cctgtgata 
      ttaggcttg attatgttt ccgatgttg aactggaag 
      agcccaact tttttcaca aaattcaat agtttaggt 
      acgtttttg cctgtttgg agtggcagc attgccaaa 
      aggctggtt ttagggact ttcagcgtt ttgtattta 
      attgttttc gttgcaacc aaagcgggg atttggggg 
      attaacatg ccagactgg gagattgaa ttttacatg 
      aaaccgact ttctgtgcc ctcagcgga atgttatct 
      ttgagttat ttcattcac aacattatc atttcgatt 
      atgaggaac aacaaacac caagaacac aacggacgt 
      gatttgaca attgcgttc ggtttggtg actttcact 
      tatttattt atcggagtt gttttctat gtttgtttt 
      ccgttaccg aagttctgt atagaggat aatatctta 
      aataatttt aacaaatac gacgttttg acaattata 
      gctaggatt ttgttactg tttcaactg tttactgtg 
      tttcccctg atttcgtac atgttgcgt aacgacgtg 
      tttgccaac attaacatg attttcaaa aattaccga 
      tttggggaa tttacctat ccgagggtt atgacactt 
      aatttggcc attttggca atttgtatt ttatttgcg 
      tgttttctt ccaagaata gggacttta atcagatat 
      acaggggct ttaagtggt ttagtctac atttttatg 
      ttgccaagt ttgttaaaa atcgccagt ttgaagaag 
      gaaggaaga ctgacgact ccgaaacta atcttccat 
      gccgctgtt atgattctc ggcagtttt aatcttctc 
      ttacaattc tttataagt gataaataa
    • >TC015635 RB
      atggactct gaaaccgaa tcccagcct ttgatgagc 
      tcgtgttca agtcaaagt ttcggaagt actaacgaa 
      tgtggcttt aaaaatgac tctgacact gatactgag 
      gctgataga gccacgaaa atacaagca gccacaatt 
      ccacctatt ctgatccaa aaacaaaac agacaattt 
      ttgtataag tcgagctac gaacatcca gattttgag 
      gtgtattcg aacgatggt gacgactac aagaccgat 
      attttaacc ggggagaag tctctaagc ggcaaaaac 
      ggcgctccg gtcaactac gactaccgc gacccggaa 
      ttaccggaa accaacagt tctctcgtc actatattc 
      gcaatttgg aacaccaca gtgggcacc tctctcttg 
      tcaatgtcc tggggcatt gaaaaaacc ggacttttt 
      cccgcaatt ttcataaac atcgccatt gccgccatt 
      tgcttgtac acaacctat actatactg aaagttaac 
      gaaaagcat ggcgtgatt ggccaaaat ggcgaagtt 
      tgtgacttg tgtcgcgct ctgattggc cgatgggcg 
      gagatttta gccaaggtg ttctcgctc gtggttctg 
      attggggca aatattgtt tattggatt ttaatgtcg 
      aattttttc tatcactcc gtccaattt ttttacgga 
      attttgcta catttggac gacactccc gtctcgaat 
      gtcacagtt ttgtgtccc aaaaacgaa acaatcgtc 
      caaatcgcc accagtacg ccgaccaac caattcgcc 
      cgaatctgg gacttgtat tcgaccgta cctgtgata 
      ttaggcttg attatgttt ccgatgttg aactggaag 
      agcccaact tttttcaca aaattcaat agtttaggt 
      acgtttttg cctgtttgg agtggcagc attgccaaa 
      aggctggtt ttagggact ttcagcgtt ttgtattta 
      attgttttc gttgcaacc aaagcgggg atttggggg 
      attaacatg ccagactgg gagattgaa ttttacatg 
      aaaccgact ttctgtgcc ctcagcgga atgttatct 
      ttgagttat ttcattcac aacattatc atttcgatt 
      atgaggaac aacaaacac caagaacac aacggacgt 
      gatttgaca attgcgttc ggtttggtg actttcact 
      tatttattt atcggagtt gttttctat gtttgtttt 
      ccgttaccg aagttctgt atagaggat cagaagatg 
      gatgaggag aacaatggt caaattgct aattatctc 
      ttggtgtgc ccaagagat tgcgaagat cataccagt 
      gtccttcaa ctgttccgc agactcaag ccacctaat 
      tgccctttt atagcgacc agggtctgg agtcgggac 
      acccgaaaa caaaaatgc tgtatgtgg ggtgtgtta 
      tatcaaagt attgatgaa acaccatat ttacaaggc 
      acacaagct aaagaacta agaaaacag ttgaatgca 
      tggagacca ataggtgga gaaattgta gattatgaa 
      ttctaccaa aatcaagcc accaatgaa agtgggtgg 
      gtgttcaca tggaggagg ttggcgtgt acccatcaa 
      ccccatttt cctaagcaa gacaatact cgaacagag 
      gagctcaaa atgtacatg tag
    • >TC015635 RC
      atggatgag gagaacaat ggtcaaatt gctaattat 
      ctcttggtg tgcccaaga gattgcgaa gatcatacc 
      agtgtcctt caactgttc cgcagactc aagccacct 
      aattgccct tttatagcg accagggtc tggagtcgg 
      gacacccga aaacaaaaa tgctgtatg tggggtgtg 
      ttatatcaa agtattgat gaaacacca tatttacaa 
      ggcacacaa gctaaagaa ctaagaaaa cagttgaat 
      gcatggaga ccaataggt ggagaaatt gtagattat 
      gaattctac caaaatcaa gccaccaat gaaagtggg 
      tgggtgttc acatggagg aggttggcg tgtacccat 
      caaccccat tttcctaag caagacaat actcgaaca 
      gaggagctc aaaatgtac atgtag
    mRNA sequence
    • >TC015635 RA
      gataaatct gaaaaaaaa aatcacaaa ataacaaac 
      cttctctta tcaaagttt gttcttatc acgatgcat 
      attaaacga agccagaaa gtggtcagt gttagtggc 
      acgcagaac gttcttcgt tatctcacg aattttcga 
      agataagtc gtcgtttcg aaacaatcg accaaacac 
      ctcttgtgt aattatgaa tggaacttt tacttgcaa 
      aattgttag tgcggtttt tgtaaaaca gttcgtcaa 
      gattttcat gttgtttta tgttgtttt attctgacg 
      tcatgatga gagatttaa tccgaaatt acaaactat 
      tactccaac gaaaatccg acatggact ctgaaaccg 
      aatcccagc ctttgatga gctcgtgtt caagtcaaa 
      gtttcggaa gtactaacg aatgtggct ttaaaaatg 
      actctgaca ctgatactg aggctgata gagccacga 
      aaatacaag cagccacaa ttccaccta ttctgatcc 
      aaaaacaaa acagacaat ttttgtata agtcgagct 
      acgaacatc cagattttg aggtgtatt cgaacgatg 
      gtgacgact acaagaccg atattttaa ccggggaga 
      agtctctaa gcggcaaaa acggcgctc cggtcaact 
      acgactacc gcgacccgg aattaccgg aaaccaaca 
      gttctctcg tcactatat tcgcaattt ggaacacca 
      cagtgggca cctctctct tgtcaatgt cctggggca 
      ttgaaaaaa ccggacttt ttcccgcaa ttttcataa 
      acatcgcca ttgccgcca tttgcttgt acacaacct 
      atactatac tgaaagtta acgaaaagc atggcgtga 
      ttggccaaa atggcgaag tttgtgact tgtgtcgcg 
      ctctgattg gccgatggg cggagattt tagccaagg 
      tgttctcgc tcgtggttc tgattgggg caaatattg 
      tttattgga ttttaatgt cgaattttt tctatcact 
      ccgtccaat ttttttacg gaattttgc tacatttgg 
      acgacactc ccgtctcga atgtcacag ttttgtgtc 
      ccaaaaacg aaacaatcg tccaaatcg ccaccagta 
      cgccgacca accaattcg cccgaatct gggacttgt 
      attcgaccg tacctgtga tattaggct tgattatgt 
      ttccgatgt tgaactgga agagcccaa cttttttca 
      caaaattca atagtttag gtacgtttt tgcctgttt 
      ggagtggca gcattgcca aaaggctgg ttttaggga 
      ctttcagcg ttttgtatt taattgttt tcgttgcaa 
      ccaaagcgg ggatttggg ggattaaca tgccagact 
      gggagattg aattttaca tgaaaccga ctttctgtg 
      ccctcagcg gaatgttat ctttgagtt atttcattc 
      acaacatta tcatttcga ttatgagga acaacaaac 
      accaagaac acaacggac gtgatttga caattgcgt 
      tcggtttgg tgactttca cttatttat ttatcggag 
      ttgttttct atgtttgtt ttccgttac cgaagttct 
      gtatagagg ataatatct taaataatt ttaacaaat 
      acgacgttt tgacaatta tagctagga ttttgttac 
      tgtttcaac tgtttactg tgtttcccc tgatttcgt 
      acatgttgc gtaacgacg tgtttgcca acattaaca 
      tgattttca aaaattacc gatttgggg aatttacct 
      atccgaggg ttatgacac ttaatttgg ccattttgg 
      caatttgta ttttatttg cgtgttttc ttccaagaa 
      tagggactt taatcagat atacagggg ctttaagtg 
      gtttagtct acattttta tgttgccaa gtttgttaa 
      aaatcgcca gtttgaaga aggaaggaa gactgacga 
      ctccgaaac taatcttcc atgccgctg ttatgattc 
      tcggcagtt ttaatcttc tcttacaat tctttataa 
      gtgataaat aatttttta ttgttgtta tgactgatt 
      ctaataaaa tatttttac aaaa
    • >TC015635 RB
      gataaatct gaaaaaaaa aatcacaaa ataacaaac 
      cttctctta tcaaagttt gttcttatc acgatgcat 
      attaaacga agccagaaa gtggtcagt gttagtggc 
      acgcagaac gttcttcgt tatctcacg aattttcga 
      agataagtc gtcgtttcg aaacaatcg accaaacac 
      ctcttgtgt aattatgaa tggaacttt tacttgcaa 
      aattgttag tgcggtttt tgtaaaaca gttcgtcaa 
      gattttcat gttgtttta tgttgtttt attctgacg 
      tcatgatga gagatttaa tccgaaatt acaaactat 
      tactccaac gaaaatccg acatggact ctgaaaccg 
      aatcccagc ctttgatga gctcgtgtt caagtcaaa 
      gtttcggaa gtactaacg aatgtggct ttaaaaatg 
      actctgaca ctgatactg aggctgata gagccacga 
      aaatacaag cagccacaa ttccaccta ttctgatcc 
      aaaaacaaa acagacaat ttttgtata agtcgagct 
      acgaacatc cagattttg aggtgtatt cgaacgatg 
      gtgacgact acaagaccg atattttaa ccggggaga 
      agtctctaa gcggcaaaa acggcgctc cggtcaact 
      acgactacc gcgacccgg aattaccgg aaaccaaca 
      gttctctcg tcactatat tcgcaattt ggaacacca 
      cagtgggca cctctctct tgtcaatgt cctggggca 
      ttgaaaaaa ccggacttt ttcccgcaa ttttcataa 
      acatcgcca ttgccgcca tttgcttgt acacaacct 
      atactatac tgaaagtta acgaaaagc atggcgtga 
      ttggccaaa atggcgaag tttgtgact tgtgtcgcg 
      ctctgattg gccgatggg cggagattt tagccaagg 
      tgttctcgc tcgtggttc tgattgggg caaatattg 
      tttattgga ttttaatgt cgaattttt tctatcact 
      ccgtccaat ttttttacg gaattttgc tacatttgg 
      acgacactc ccgtctcga atgtcacag ttttgtgtc 
      ccaaaaacg aaacaatcg tccaaatcg ccaccagta 
      cgccgacca accaattcg cccgaatct gggacttgt 
      attcgaccg tacctgtga tattaggct tgattatgt 
      ttccgatgt tgaactgga agagcccaa cttttttca 
      caaaattca atagtttag gtacgtttt tgcctgttt 
      ggagtggca gcattgcca aaaggctgg ttttaggga 
      ctttcagcg ttttgtatt taattgttt tcgttgcaa 
      ccaaagcgg ggatttggg ggattaaca tgccagact 
      gggagattg aattttaca tgaaaccga ctttctgtg 
      ccctcagcg gaatgttat ctttgagtt atttcattc 
      acaacatta tcatttcga ttatgagga acaacaaac 
      accaagaac acaacggac gtgatttga caattgcgt 
      tcggtttgg tgactttca cttatttat ttatcggag 
      ttgttttct atgtttgtt ttccgttac cgaagttct 
      gtatagagg atcagaaga tggatgagg agaacaatg 
      gtcaaattg ctaattatc tcttggtgt gcccaagag 
      attgcgaag atcatacca gtgtccttc aactgttcc 
      gcagactca agccaccta attgccctt ttatagcga 
      ccagggtct ggagtcggg acacccgaa aacaaaaat 
      gctgtatgt ggggtgtgt tatatcaaa gtattgatg 
      aaacaccat atttacaag gcacacaag ctaaagaac 
      taagaaaac agttgaatg catggagac caataggtg 
      gagaaattg tagattatg aattctacc aaaatcaag 
      ccaccaatg aaagtgggt gggtgttca catggagga 
      ggttggcgt gtacccatc aaccccatt ttcctaagc 
      aagacaata ctcgaacag aggagctca aaatgtaca 
      tgtagtgat agtaaataa ttatggtat tttttaata 
      aaagtgtgg aatccctta attagtatt tatgatgta 
      cctactcac acataaata aagaaatca agactta
    • >TC015635 RC
      acttcctgc caatgctcg ttcagttgc ttcagtcct 
      gtgcaagtc ctgtggttc agtctatgc atagatcct 
      ccattatct ataattcta tgctgtgat tctatctta 
      tcagtcaac cagaagatg gatgaggag aacaatggt 
      caaattgct aattatctc ttggtgtgc ccaagagat 
      tgcgaagat cataccagt gtccttcaa ctgttccgc 
      agactcaag ccacctaat tgccctttt atagcgacc 
      agggtctgg agtcgggac acccgaaaa caaaaatgc 
      tgtatgtgg ggtgtgtta tatcaaagt attgatgaa 
      acaccatat ttacaaggc acacaagct aaagaacta 
      agaaaacag ttgaatgca tggagacca ataggtgga 
      gaaattgta gattatgaa ttctaccaa aatcaagcc 
      accaatgaa agtgggtgg gtgttcaca tggaggagg 
      ttggcgtgt acccatcaa ccccatttt cctaagcaa 
      gacaatact cgaacagag gagctcaaa atgtacatg 
      tagtgatag taaataatt atggtattt tttaataaa 
      agtgtggaa tcccttaat tagtattta tgatgtacc 
      tactcacac ataaataaa gaaatcaag actta
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