Status
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Gene information
General information
TC033406
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015615,TC015616
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3467556..3470199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033406
    iBeetle screen iB_08530

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033406 RA
      MKQTFLEAG DEILYDVIK DFDRNEKSF NEDVTVVTK 
      WLQEQPHLP EVLDKRVIQ NFLLLSKCS VEKTKQKID 
      MYFTLRSKL EDVFGESNP KLPHLKEIR EVMNFVVYP 
      KTFKNNRLF IFKMRQENL GEKLEPMNV LRHLLSIHE 
      IRMKEDVTF GEYFIVDCS HVPVSYILK LTPTFVFKA 
      VAIIYEKLY SARLKGLYL VNFPKFGEA ALTLAQKIM 
      KPKLFERIK VCPDLDSLK DVFPEDVLP AEFGGTGYT 
      LQQLRDMLE SKLGEYQCW YDHLDTLRV DEKLRPESL 
      KNDDILGFH GNFKKLDNG EGLGNYYFT HSHFLAIMF 
      ATSADALKT ILKLYDQNE ESLRLDITK LKNWMKSQG 
      HLPEILDDK RIENFLILN KFSIERTKQ SIDMYYTVR 
      TLIPELYEH VNPQKPNMA EARQIAYWL ALPKMTDEM 
      HRVMVFKMR NKQMIDKME PRNVIGLVI NLEELRLNE 
      DIMFGDVTI FDNEGINFG YFKKLTPSI IAKVLTVYE 
      KVFSIPMKA IYIVNSPPF VSTILAILK SLMKPKLFE 
      RIHVCEDTS ILTEKIPKR ILPRDYGGE EKSLEELQG 
      GQHKSPLVI F
    CDS sequence
    • >TC033406 RA
      atgaagcag actttcctc gaagccggt gacgagatt 
      ctttacgac gtgatcaaa gactttgat cgtaatgaa 
      aaatcgttc aatgaggac gttacagtt gtgacaaaa 
      tggctccaa gagcagccc cacctaccg gaagttttg 
      gataaacgg gtcattcag aatttcctt ttgctaagt 
      aaatgtagc gtcgaaaaa acgaagcaa aaaatcgac 
      atgtatttc accctacgc tcaaaactg gaagatgtg 
      ttcggcgaa agtaacccg aaactaccc cacttgaag 
      gaaattcgt gaagttatg aattttgtc gtttacccg 
      aaaactttc aaaaacaat cgccttttt attttcaaa 
      atgcggcaa gagaatttg ggggaaaaa ctcgaaccg 
      atgaacgtg ctgcgccac ctgttgtcc attcacgaa 
      atacgaatg aaggaagat gtgactttt ggcgagtat 
      tttatcgtg gattgtagc cacgtccca gtgtcttac 
      attttaaaa ttaacgccg acttttgtc ttcaaagcc 
      gtggccata atttacgaa aaattgtac tcagcgcgt 
      ctgaaagga ctctacctt gttaatttt cccaaattc 
      ggggaggcg gccctgact ttggcccaa aaaatcatg 
      aaaccgaag ctgttcgag aggatcaaa gtgtgcccc 
      gacttggac tccctcaaa gacgtgttt cctgaagac 
      gtacttccg gccgagttt ggaggaacc ggatatacg 
      ctccaacaa ctgcgagat atgttggag agtaaatta 
      ggtgaatac caatgttgg tacgaccac ttggacacg 
      ctccgagtg gacgagaaa ttgcgtccc gaaagttta 
      aaaaatgac gacattttg ggatttcat ggcaacttt 
      aaaaaactc gataacggt gaaggactt ggtaattat 
      tacttcact cacagtcac tttctagcc atcatgttt 
      gccacaagt gccgacgct ttgaaaaca atcctaaaa 
      ctctacgac caaaatgaa gaaagtctt cgacttgac 
      ataacaaag ttgaagaat tggatgaaa agtcaaggc 
      cacctcccc gaaattttg gacgacaaa agaattgag 
      aatttccta atcctcaat aaattcagt atcgagcgg 
      acgaaacaa agtatcgat atgtattac acagtccgt 
      actttaatt ccggagttg tacgaacac gtcaatccg 
      caaaagccg aacatggcc gaagcaagg caaatcgcc 
      tattggctg gcgctcccg aaaatgacc gacgagatg 
      caccgagtg atggttttc aaaatgcgc aacaaacaa 
      atgattgac aagatggag ccgcgaaat gttatcgga 
      cttgtcatt aatttggaa gaattgcga ttaaatgaa 
      gacataatg tttggtgac gtcaccatt tttgacaac 
      gagggcatc aatttcggc tacttcaaa aaactgaca 
      cccagtatc attgccaaa gttttgaca gtttacgaa 
      aaagtgttc tcaatccca atgaaggcc atctacatt 
      gttaacagc ccgccgttc gttagcaca attctggca 
      atcttgaaa agcttaatg aaaccaaaa ctgttcgaa 
      cgcattcac gtgtgcgaa gatacgagt attttgaca 
      gaaaaaatc cccaaacgc attctcccg cgcgattac 
      ggaggcgag gaaaagtcc ctagaggag ttacaaggt 
      ggccaacat aaaagccca ctggtaatt ttctaa
    mRNA sequence
    • >TC033406 RA
      cagtcattg tcaaaatga agcagactt tcctcgaag 
      ccggtgacg agattcttt acgacgtga tcaaagact 
      ttgatcgta atgaaaaat cgttcaatg aggacgtta 
      cagttgtga caaaatggc tccaagagc agccccacc 
      taccggaag ttttggata aacgggtca ttcagaatt 
      tccttttgc taagtaaat gtagcgtcg aaaaaacga 
      agcaaaaaa tcgacatgt atttcaccc tacgctcaa 
      aactggaag atgtgttcg gcgaaagta acccgaaac 
      taccccact tgaaggaaa ttcgtgaag ttatgaatt 
      ttgtcgttt acccgaaaa ctttcaaaa acaatcgcc 
      tttttattt tcaaaatgc ggcaagaga atttggggg 
      aaaaactcg aaccgatga acgtgctgc gccacctgt 
      tgtccattc acgaaatac gaatgaagg aagatgtga 
      cttttggcg agtatttta tcgtggatt gtagccacg 
      tcccagtgt cttacattt taaaattaa cgccgactt 
      ttgtcttca aagccgtgg ccataattt acgaaaaat 
      tgtactcag cgcgtctga aaggactct accttgtta 
      attttccca aattcgggg aggcggccc tgactttgg 
      cccaaaaaa tcatgaaac cgaagctgt tcgagagga 
      tcaaagtgt gccccgact tggactccc tcaaagacg 
      tgtttcctg aagacgtac ttccggccg agtttggag 
      gaaccggat atacgctcc aacaactgc gagatatgt 
      tggagagta aattaggtg aataccaat gttggtacg 
      accacttgg acacgctcc gagtggacg agaaattgc 
      gtcccgaaa gtttaaaaa atgacgaca ttttgggat 
      ttcatggca actttaaaa aactcgata acggtgaag 
      gacttggta attattact tcactcaca gtcactttc 
      tagccatca tgtttgcca caagtgccg acgctttga 
      aaacaatcc taaaactct acgaccaaa atgaagaaa 
      gtcttcgac ttgacataa caaagttga agaattgga 
      tgaaaagtc aaggccacc tccccgaaa ttttggacg 
      acaaaagaa ttgagaatt tcctaatcc tcaataaat 
      tcagtatcg agcggacga aacaaagta tcgatatgt 
      attacacag tccgtactt taattccgg agttgtacg 
      aacacgtca atccgcaaa agccgaaca tggccgaag 
      caaggcaaa tcgcctatt ggctggcgc tcccgaaaa 
      tgaccgacg agatgcacc gagtgatgg ttttcaaaa 
      tgcgcaaca aacaaatga ttgacaaga tggagccgc 
      gaaatgtta tcggacttg tcattaatt tggaagaat 
      tgcgattaa atgaagaca taatgtttg gtgacgtca 
      ccatttttg acaacgagg gcatcaatt tcggctact 
      tcaaaaaac tgacaccca gtatcattg ccaaagttt 
      tgacagttt acgaaaaag tgttctcaa tcccaatga 
      aggccatct acattgtta acagcccgc cgttcgtta 
      gcacaattc tggcaatct tgaaaagct taatgaaac 
      caaaactgt tcgaacgca ttcacgtgt gcgaagata 
      cgagtattt tgacagaaa aaatcccca aacgcattc 
      tcccgcgcg attacggag gcgaggaaa agtccctag 
      aggagttac aaggtggcc aacataaaa gcccactgg 
      taattttct aactacgtt ttttagggt tattggacc 
      tgaagttgg cacagtatc aggacaggt ttgacaaac 
      tggaccaac ttcacgtcg atgagtcac aaaggcctg 
      agaagttga aaaacgacg aaattttgg gatttcatg 
      gcaatttta agaaattga gtgtcgatt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY