Status
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Gene information
General information
TC015614 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3462176..3465601
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015614
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08529

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015614 RA
      MPPNQSTVT NTPSLRIAI IGQSPFAVD VLKRLRQHG 
      HVIVGVFTI PDKGTREDP LAKIAHECD IPLFKVKAW 
      RKSGTPLPE VLANYRSVN ADLNVLPYC SQFIPMEVI 
      NYPRLKTIC YHPSLLPRH RGASSINWT LISGDKKAG 
      FSIFWADDG LDTGPILLQ EECDVYEDD TVDSLYNRF 
      LYPAGVTAL ARAVDLVAD GTAPREPQS EKGATYDPM 
      LNKPELCQI DWGKTGPEL HDFIRGMDS VPGASCRVK 
      VNDKYHEVA LFGSSVWKG GKPQGLDVG IEGGPNGTI 
      HEDGLILTG SDGTMVNVK RVKIEGRVK LASNLLQTG 
      QQVTIELTE DEKSQIESM RGIWKSILN TEIEDETDF 
      FASGAGSMD VVRLVEEIK DLLKLEDYQ NEDVFLNPV 
      FTEFSLNAV LKKRGGETP KEINYKGVE IEANGLKIK 
      VPCQLFING EFVDAENGK TLPTVNPTT EETICQVQC 
      GSAKDVDRA VQAAKKAFY EGEWSKISA RDRGQLLFK 
      LADLMHQHR EELATIESI DSGAVYTLA LKTHVGMSI 
      ETWRYFAGW ADKIHGSTI PVSHARPNK NLSFTKREP 
      IGVCGLITP WNYPLMMLS WKMAACLAA GNTVIIKPA 
      QVCPLTALK FAELAVKAG IPPGVINVV PGTGSTTGQ 
      AIATHPEVR KLGFTGSTG IGQVIMKSC AESNLKKVS 
      LELGGKSPL VIFADCDLD KAVRLGMQS VFFNKGENC 
      IAAGRLFVE ESIHDEFIR RVVTETKKI TIGDPLNKS 
      TAHGPQNHQ AHLTKLLDY IKMGVKEGA KLEYGGERL 
      NRKGYFLQP TIFSHVEDH MYIAKEESF GPIMVISKF 
      SNGDIDGVL KRANATEYG LASGVFTRD LSKALKFAE 
      KVEAGTVFV NTYNKTDVA APFGGFKQS GFGKDLGQD 
      ALNEYLKTK CVTIEY
    CDS sequence
    • >TC015614 RA
      atgccacca aatcaaagc acagtcacc aacacgcca 
      tccctgcgc attgcgatc atagggcag agccctttc 
      gccgtcgac gtcctgaaa cggctacgg caacacggg 
      cacgtcatc gtgggggtg ttcacaatc cctgacaag 
      ggcacccgg gaagatccc ttagctaaa atcgcccac 
      gagtgcgac atcccctta ttcaaggtc aaggcgtgg 
      cgtaaatcg ggcacgccc ctccccgaa gtcctagcc 
      aattaccgc agcgtcaac gccgacttg aacgtcctc 
      ccctactgc tcgcaattc atcccaatg gaagtcata 
      aactatccc cgcctcaaa acgatatgc taccacccg 
      tcgctcctc ccacgccac cgcggcgcc agttccatc 
      aactggacg ctcatttcc ggcgataaa aaggcgggg 
      tttagcatt ttctgggcg gacgatggg ctggacacc 
      ggaccaatc ctgctacag gaggagtgt gacgtttac 
      gaggacgac accgttgat tcgctctat aaccggttt 
      ttgtacccg gccggagtg accgccctt gctagggcc 
      gttgacctt gtagctgac ggtaccgcc cccagggaa 
      ccccagagt gagaaaggc gccacctat gaccccatg 
      ttaaacaaa ccggaactt tgccaaatc gattggggg 
      aaaaccggg ccggagttg cacgatttt atccgaggg 
      atggattcg gtcccgggg gcgtcatgt cgggtcaaa 
      gtcaatgat aagtaccac gaagttgca ctttttggg 
      tcaagtgtt tggaaaggg gggaagccc caaggactg 
      gacgttggg attgaaggt ggtcccaat ggtaccata 
      cacgaggat gggttaatt ttgacgggt tctgatggt 
      accatggtc aatgtcaag cgggtcaaa attgaggga 
      cgggtcaag cttgcctca aacttgctc caaaccggt 
      caacaagtc acaattgag ttgacggag gatgagaaa 
      agtcaaatt gagtcaatg aggggcatt tggaagtca 
      atcctcaat acggaaatt gaggatgag accgatttt 
      ttcgcctca ggggccggg tcaatggac gtcgtccga 
      ttggttgag gaaattaag gatttactc aaacttgag 
      gattatcaa aatgaggat gttttcctc aatccggtt 
      ttcaccgaa ttttcgttg aacgccgtt ttgaaaaaa 
      aggggcggg gaaaccccc aaagaaatc aactataag 
      ggcgttgaa atcgaagct aatggactc aaaatcaaa 
      gttccgtgc caacttttc atcaatggg gagtttgtt 
      gacgcggaa aatggaaaa accctacca actgtcaac 
      ccaaccacc gaagaaacc atctgtcag gtccaatgt 
      ggctcagcc aaggacgtg gacagggcc gtccaagcg 
      gccaaaaag gccttttac gagggagaa tggagcaaa 
      atcagcgca cgtgaccgc ggccaactc cttttcaag 
      ttggcagac ctgatgcac caacaccgc gaagagttg 
      gccacgatc gagtcaatc gactcgggg gcggtttac 
      accctagcc ctgaagacg cacgtcggg atgagcatc 
      gaaacgtgg cgatacttc gccggatgg gcggacaaa 
      atccacgga tcgaccatc ccagtttcg cacgcccgc 
      cccaataaa aacctctcc tttacgaag agagaaccg 
      attggagtc tgcggcctg atcacgccc tggaactac 
      ccacttatg atgctatcg tggaaaatg gcagcgtgc 
      cttgcggcc ggtaataca gttatcatc aaaccggca 
      caagtgtgc cctctaacc gcacttaaa ttcgccgaa 
      ttggcggtc aaggcgggg attcccccg ggtgtgatc 
      aacgtcgtg ccgggcacg gggtcaacg accggccaa 
      gcgatcgcg acgcatccc gaagtacgc aagttgggt 
      tttacggga agtaccgga atcgggcaa gtcattatg 
      aagtcgtgc gcggaatca aacttgaag aaagtgtcg 
      cttgaactg ggggggaag tccccactg gttattttc 
      gccgactgc gatctggac aaggccgtc cgattggga 
      atgcaaagt gtttttttc aacaagggc gagaattgc 
      atagctgcc gggcgactt ttcgtggag gaaagcatc 
      cacgacgag tttattcga cgcgtggtc accgaaacc 
      aaaaaaata acaattggg gatcccctg aacaaatca 
      acagcgcac ggcccacaa aatcaccaa gcacacttg 
      accaaacta ctcgattat attaaaatg ggggtcaaa 
      gagggggcg aaattggaa tatggaggg gagcggtta 
      aaccgaaag ggttacttc ctccaaccg accattttc 
      agccacgtg gaggaccac atgtacatc gccaaggag 
      gaatcgttt gggcccatc atggttatt agtaaattt 
      agcaatggg gacattgac ggtgtttta aaacgtgca 
      aacgcgact gaatatggg ctcgcctcc ggggttttt 
      acccgagat ttgtccaaa gcgctcaaa tttgcggaa 
      aaagtcgaa gccgggact gtttttgtc aacacttat 
      aataaaacg gacgtcgcg gcgccgttt ggggggttc 
      aagcagtcg ggatttggc aaagattta ggacaagac 
      gctctgaac gaatatttg aaaacgaaa tgcgtcaca 
      attgagtat taa
    mRNA sequence
    • >TC015614 RA
      acagccaca caaaatgcc accaaatca aagcacagt 
      caccaacac gccatccct gcgcattgc gatcatagg 
      gcagagccc tttcgccgt cgacgtcct gaaacggct 
      acggcaaca cgggcacgt catcgtggg ggtgttcac 
      aatccctga caagggcac ccgggaaga tcccttagc 
      taaaatcgc ccacgagtg cgacatccc cttattcaa 
      ggtcaaggc gtggcgtaa atcgggcac gcccctccc 
      cgaagtcct agccaatta ccgcagcgt caacgccga 
      cttgaacgt cctccccta ctgctcgca attcatccc 
      aatggaagt cataaacta tccccgcct caaaacgat 
      atgctacca cccgtcgct cctcccacg ccaccgcgg 
      cgccagttc catcaactg gacgctcat ttccggcga 
      taaaaaggc ggggtttag cattttctg ggcggacga 
      tgggctgga caccggacc aatcctgct acaggagga 
      gtgtgacgt ttacgagga cgacaccgt tgattcgct 
      ctataaccg gtttttgta cccggccgg agtgaccgc 
      ccttgctag ggccgttga ccttgtagc tgacggtac 
      cgcccccag ggaacccca gagtgagaa aggcgccac 
      ctatgaccc catgttaaa caaaccgga actttgcca 
      aatcgattg ggggaaaac cgggccgga gttgcacga 
      ttttatccg agggatgga ttcggtccc gggggcgtc 
      atgtcgggt caaagtcaa tgataagta ccacgaagt 
      tgcactttt tgggtcaag tgtttggaa aggggggaa 
      gccccaagg actggacgt tgggattga aggtggtcc 
      caatggtac catacacga ggatgggtt aattttgac 
      gggttctga tggtaccat ggtcaatgt caagcgggt 
      caaaattga gggacgggt caagcttgc ctcaaactt 
      gctccaaac cggtcaaca agtcacaat tgagttgac 
      ggaggatga gaaaagtca aattgagtc aatgagggg 
      catttggaa gtcaatcct caatacgga aattgagga 
      tgagaccga ttttttcgc ctcaggggc cgggtcaat 
      ggacgtcgt ccgattggt tgaggaaat taaggattt 
      actcaaact tgaggatta tcaaaatga ggatgtttt 
      cctcaatcc ggttttcac cgaattttc gttgaacgc 
      cgttttgaa aaaaagggg cggggaaac ccccaaaga 
      aatcaacta taagggcgt tgaaatcga agctaatgg 
      actcaaaat caaagttcc gtgccaact tttcatcaa 
      tggggagtt tgttgacgc ggaaaatgg aaaaaccct 
      accaactgt caacccaac caccgaaga aaccatctg 
      tcaggtcca atgtggctc agccaagga cgtggacag 
      ggccgtcca agcggccaa aaaggcctt ttacgaggg 
      agaatggag caaaatcag cgcacgtga ccgcggcca 
      actcctttt caagttggc agacctgat gcaccaaca 
      ccgcgaaga gttggccac gatcgagtc aatcgactc 
      gggggcggt ttacaccct agccctgaa gacgcacgt 
      cgggatgag catcgaaac gtggcgata cttcgccgg 
      atgggcgga caaaatcca cggatcgac catcccagt 
      ttcgcacgc ccgccccaa taaaaacct ctcctttac 
      gaagagaga accgattgg agtctgcgg cctgatcac 
      gccctggaa ctacccact tatgatgct atcgtggaa 
      aatggcagc gtgccttgc ggccggtaa tacagttat 
      catcaaacc ggcacaagt gtgccctct aaccgcact 
      taaattcgc cgaattggc ggtcaaggc ggggattcc 
      cccgggtgt gatcaacgt cgtgccggg cacggggtc 
      aacgaccgg ccaagcgat cgcgacgca tcccgaagt 
      acgcaagtt gggttttac gggaagtac cggaatcgg 
      gcaagtcat tatgaagtc gtgcgcgga atcaaactt 
      gaagaaagt gtcgcttga actgggggg gaagtcccc 
      actggttat tttcgccga ctgcgatct ggacaaggc 
      cgtccgatt gggaatgca aagtgtttt tttcaacaa 
      gggcgagaa ttgcatagc tgccgggcg acttttcgt 
      ggaggaaag catccacga cgagtttat tcgacgcgt 
      ggtcaccga aaccaaaaa aataacaat tggggatcc 
      cctgaacaa atcaacagc gcacggccc acaaaatca 
      ccaagcaca cttgaccaa actactcga ttatattaa 
      aatgggggt caaagaggg ggcgaaatt ggaatatgg 
      aggggagcg gttaaaccg aaagggtta cttcctcca 
      accgaccat tttcagcca cgtggagga ccacatgta 
      catcgccaa ggaggaatc gtttgggcc catcatggt 
      tattagtaa atttagcaa tggggacat tgacggtgt 
      tttaaaacg tgcaaacgc gactgaata tgggctcgc 
      ctccggggt ttttacccg agatttgtc caaagcgct 
      caaatttgc ggaaaaagt cgaagccgg gactgtttt 
      tgtcaacac ttataataa aacggacgt cgcggcgcc 
      gtttggggg gttcaagca gtcgggatt tggcaaaga 
      tttaggaca agacgctct gaacgaata tttgaaaac 
      gaaatgcgt cacaattga gtattaagt aggtttaag 
      gccgtattt attgtaatt ttaatttaa ttaataaac 
      gcttgtata gatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY