Status
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Gene information
General information
TC030906
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015105,TC015106
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3258103..3280686
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030906
    iBeetle screen iB_08524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Adult morphology
    motility decreased - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    previtellogenic egg chamber sometimes number increased - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030906 RA
      MGSLPNLHE LGKDVENGP SPYKPGPAL GHLCSGGNS 
      TLSQAEKTK ILHRYSGHA RITKARSNG NESKIVCDD 
      FMLEHMATY SPISNRSTR NLTHNIRRR DSLNSSIGA 
      TSVRRLIAV VRSNAHHTT QTRQGPPYS RRLLLSNLV 
      VLCITHLLI TAAFLPFLA LQTSVSVWT MPLGHYLTP 
      IHINIGSIL LALAHFFAA VSALLGPSV VQKLGTNVV 
      FFCSYLVFV VFYASHLYP SLYVLFPGA VLLGVALGP 
      LVSAQITFL MTLSAKINY MFSEEDDDS KALRRTCII 
      RRLARAFQA AHDFGLIVG SVLSALLIT YTVNINNQE 
      YGDIFDNST AVMEKNNQT ESCSNLTLE INCTREKNS 
      LSYNIYDYN AFLDDIFDT FEGTRLCGA QACPPNFLL 
      HFNMSEESY FHVLPKKSS MVLAVVYAG LCFVAAMAS 
      AVGLDRIRI FVYQDPLER PEGLAALRA VKESFKDVR 
      LQMAAPLAL FIGLEQAFI YADFSKSYV VCTLGIHRL 
      NLVFLSMGL LQSIAACTL SMLLRTIRR YYVVAVGFT 
      FHACLLMVL ILWKPIEDD PALFYVISA AWGVCNAIW 
      EMLNFTLLT GLYSDNWES AFANSAFFR FLGLSIAFA 
      FHGLLCNYY KLYALGFFL LTAVVPFVW LEVRLENMR 
      KVKNIIRL
    CDS sequence
    • >TC030906 RA
      atgggcagt ttaccaaat ttgcacgaa ctaggaaaa 
      gacgtcgag aatggtcca tcgccttac aagcctggt 
      ccagccctg ggtcacctt tgtagtggc ggcaactct 
      acattatcc caagctgag aaaacaaaa attttgcac 
      cgatattcg ggacatgcc aggataact aaagctaga 
      agcaacggc aatgagtcc aagattgtc tgcgatgac 
      tttatgcta gagcacatg gccacctat tcgccaatt 
      tccaaccga tcaacccgg aatctgacc cacaacatc 
      cgccgtcgc gactcactg aacagctca ataggggcc 
      acatctgtg cgtcgtttg atcgccgtc gtgcgctcc 
      aacgcccac cacaccacc cagaccaga caagggccc 
      ccatactcc cgccgcctg ctcctctcg aacctggtg 
      gtgctgtgc atcacgcac ctgcttatc actgccgcc 
      ttcttgcct ttcctcgcc ctccagacc agcgtctcg 
      gtgtggacg atgccgctg gggcactat ctcacgccc 
      atccacatc aacatcggc tccatcctc ctggccctg 
      gcgcacttc ttcgccgcc gtgtcggcg ctcctcggc 
      ccctccgtc gtgcaaaag ctgggcacg aacgtcgtc 
      ttcttctgc tcctatctg gtgtttgtc gttttctac 
      gcctcacat ctctaccca tcgttgtat gtgcttttc 
      cccggcgct gtgctgctg ggagtggct ttggggccg 
      ctggtctcg gcgcaaatc accttcctg atgacgctc 
      tcggccaag attaactac atgttctcg gaggaggac 
      gacgattcg aaagcgttg cgacggacg tgtattatc 
      aggaggttg gcgagagcg tttcaagca gctcatgat 
      tttggactt attgttggc tcggtttta tcggcgtta 
      cttattact tacactgtc aatattaac aatcaagaa 
      tatggtgat attttcgac aattccacg gcggtcatg 
      gagaaaaat aatcaaact gaaagttgt agcaatttg 
      actttagaa attaactgc accagagaa aaaaattcc 
      ctttcgtac aacatctac gactacaat gccttcttg 
      gacgacata ttcgacact tttgaaggg acgcgactg 
      tgtggagcc caagcgtgt cccccgaat tttctcctg 
      cactttaac atgtcagaa gagagttat tttcatgtt 
      ttgccgaag aaaagttcc atggttttg gctgtggtg 
      tatgcagga ctttgcttc gttgcagcg atggctagt 
      gctgtaggg ttggataga attcggatc tttgtatat 
      caagatcca ctggagagg ccagaaggg ttagctgcc 
      ttaagagct gtgaaggag tctttcaaa gatgtgagg 
      ctgcaaatg gccgcgcct ttggcgctg tttattgga 
      ctggagcaa gctttcatc tatgctgac tttagcaag 
      tcttatgtg gtatgcact ttgggcatc caccgactg 
      aatttggtg ttcttgtcg atgggtctt ctgcaatcc 
      atcgctgct tgcaccctt agtatgctt ctgaggacc 
      atcagaaga tactacgtt gtggccgta ggttttacg 
      ttccacgct tgtctgtta atggtccta atcctgtgg 
      aaacccatc gaggacgat cctgccctt ttctacgtc 
      atctcggcc gcttggggc gtctgtaac gccatttgg 
      gaaatgctg aacttcacc cttttgacc ggcctctac 
      agcgacaac tgggagtct gcgttcgcc aattcggcg 
      ttcttccgt ttcctgggc ttgagcata gccttcgca 
      ttccacgga cttctctgc aactactac aaactctac 
      gccttgggc ttcttcctg ctgacggcc gtcgtcccc 
      ttcgtgtgg ctcgaagtc cggctggag aacatgcgc 
      aaagtcaag aatatcatc cggttatga
    mRNA sequence
    • >TC030906 RA
      tcgcgctac acaagagag catcacttg tttgcacct 
      tatttctta agagattca ttgcgaaat gtgcctaat 
      tgacgtgat ttagtgctt gctgattat cattttcat 
      aaacaagtg gaattgcct gcctgctca ctgattcta 
      gaccgtaac aaaatgctt gtaaggtag caagagatt 
      taatcacac ctaaaagag tgagagtaa acgtaatca 
      tgtgataaa ttccttcaa aaatgggca gtttaccaa 
      atttgcacg aactaggaa aagacgtcg agaatggtc 
      catcgcctt acaagcctg gtccagccc tgggtcacc 
      tttgtagtg gcggcaact ctacattat cccaagctg 
      agaaaacaa aaattttgc accgatatt cgggacatg 
      ccaggataa ctaaagcta gaagcaacg gcaatgagt 
      ccaagattg tctgcgatg actttatgc tagagcaca 
      tggccacct attcgccaa tttccaacc gatcaaccc 
      ggaatctga cccacaaca tccgccgtc gcgactcac 
      tgaacagct caatagggg ccacatctg tgcgtcgtt 
      tgatcgccg tcgtgcgct ccaacgccc accacacca 
      cccagacca gacaagggc ccccatact cccgccgcc 
      tgctcctct cgaacctgg tggtgctgt gcatcacgc 
      acctgctta tcactgccg ccttcttgc ctttcctcg 
      ccctccaga ccagcgtct cggtgtgga cgatgccgc 
      tggggcact atctcacgc ccatccaca tcaacatcg 
      gctccatcc tcctggccc tggcgcact tcttcgccg 
      ccgtgtcgg cgctcctcg gcccctccg tcgtgcaaa 
      agctgggca cgaacgtcg tcttcttct gctcctatc 
      tggtgtttg tcgttttct acgcctcac atctctacc 
      catcgttgt atgtgcttt tccccggcg ctgtgctgc 
      tgggagtgg ctttggggc cgctggtct cggcgcaaa 
      tcaccttcc tgatgacgc tctcggcca agattaact 
      acatgttct cggaggagg acgacgatt cgaaagcgt 
      tgcgacgga cgtgtatta tcaggaggt tggcgagag 
      cgtttcaag cagctcatg attttggac ttattgttg 
      gctcggttt tatcggcgt tacttatta cttacactg 
      tcaatatta acaatcaag aatatggtg atattttcg 
      acaattcca cggcggtca tggagaaaa ataatcaaa 
      ctgaaagtt gtagcaatt tgactttag aaattaact 
      gcaccagag aaaaaaatt ccctttcgt acaacatct 
      acgactaca atgccttct tggacgaca tattcgaca 
      cttttgaag ggacgcgac tgtgtggag cccaagcgt 
      gtcccccga attttctcc tgcacttta acatgtcag 
      aagagagtt attttcatg ttttgccga agaaaagtt 
      ccatggttt tggctgtgg tgtatgcag gactttgct 
      tcgttgcag cgatggcta gtgctgtag ggttggata 
      gaattcgga tctttgtat atcaagatc cactggaga 
      ggccagaag ggttagctg ccttaagag ctgtgaagg 
      agtctttca aagatgtga ggctgcaaa tggccgcgc 
      ctttggcgc tgtttattg gactggagc aagctttca 
      tctatgctg actttagca agtcttatg tggtatgca 
      ctttgggca tccaccgac tgaatttgg tgttcttgt 
      cgatgggtc ttctgcaat ccatcgctg cttgcaccc 
      ttagtatgc ttctgagga ccatcagaa gatactacg 
      ttgtggccg taggtttta cgttccacg cttgtctgt 
      taatggtcc taatcctgt ggaaaccca tcgaggacg 
      atcctgccc ttttctacg tcatctcgg ccgcttggg 
      gcgtctgta acgccattt gggaaatgc tgaacttca 
      cccttttga ccggcctct acagcgaca actgggagt 
      ctgcgttcg ccaattcgg cgttcttcc gtttcctgg 
      gcttgagca tagccttcg cattccacg gacttctct 
      gcaactact acaaactct acgccttgg gcttcttcc 
      tgctgacgg ccgtcgtcc ccttcgtgt ggctcgaag 
      tccggctgg agaacatgc gcaaagtca agaatatca 
      tccggttat gacctaaac tcaacattt ggtgattcc 
      tcatgtaaa catatcatt cacttgacg tcacggttt 
      tgcgtgtga tgttgtaca gagtaatgt taaaaggtt 
      gttatgaca cctcttcaa tgtatataa gattgttta 
      cttccacaa ataaaatct caacaagaa a
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