| General information | |||
| TC015139 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 2447774..2461185 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015139 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08518 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
mature egg not present - Number of animals showing the phenotype: 4
previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
vitellogenic egg chamber potentially position anteriorized - Number of animals showing the phenotype: 4
vitellarium size decreased - Number of animals showing the phenotype: 4
tropharium size decreased - Number of animals showing the phenotype: 4
tropharium circumference increased - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015139 RA
MFVFQTGYI RTSHDNYYI EPAEKSSGE YPLGSFLHR IKKIHNSAP TGNEVVVNE SDQCETNDN ESIVEDDFG SSVVAIPSH TLTKRAIVD DIGDSPFLS RKIRDADWP INYETDQKT NEYFVKVLV VADRSMIQY HQSNEDLKH YILILMSHA ALLYKDASI GNSISLSVV NIWLLNNTV FTSNNSQVM LRRFCEWQK RYVTDRKHD MALLFTRDP ICKKNSSSC DTLGVAEVG SMCNPSSSC AIVKERGLS SSYTIAHEL GHVLSMLHD ETESCSHFS RGPKSENIM SRILNNGTK PWLWSECSK HFLTEFLES NKAKCLLNA PTSNYISSE ISDLLPGEN FEADKQCEL EFGIGYKLC SYQASCAVL WCTSNDIYG CKSNLLPWA DGTSCGRNR WCHHGQCIP LDRQKLTPI PGGWGPWQP WSDCSRTCG GGVKQTRRY CDSPVPQNG GPYCTGKSI QYVSCNTES CDDDVPDFR EVQCSQFDG INKNLANLT KDVKWVPKY GLSKREDLC KLFCRPENS NAYYPLKDK VIDGTKCGL NSFDICVNG ICRPGGCDN KLNSKMDLD ECGVCGGDN SKCEEITGN YNVSMTGYN IVLRIPKGS SNIDIRQNA NNDTNYLVL VDGETGEYV LNSKTMIAP QQKDVIFAG IIISYSGSS AAIERITTP KNHKLTKDL VLGVLSVGH ALAPDITYR YIIDRENAP KYGWRLYRN KWSKCNSIC SGTQYMSPI CIELATNKE QPESFCPQH EKQHHMQKQ DCNTHCQLR WSDVSRGPC SVNCGQGVR SVYHQCVKI DMTKENYDR YHEVVDHKH CNILGKPPT MEVCQGRCE STRWEYTSW SECSTSCGG GTQRRSAKC VDNNSRTLD DSYCPGEKI TEQRCNTQK CPIWESREW TPCSVTCGR GFRTKPYFC HIDGKILDP SACDSRQKH IEEEQCYER PCAGWSIGP WGECSTTCG DGTSTRQVI CQNLDDNQV IQDSFCSGI LKPNETKFC KIADCVQSL PYQRRYDYG NDILANTIV SYERGFKWV TGAWSQCSK SCGTGVSSR MVVCRNELG EEDERYCAK SVVPVKTIE CNTGKCPAW EFGGWSGCD FNCEKRRQV TCRAASGSF VEDTQCDKH RKPSTTTKC KLSQCPHLA SKPYSPHRY RWSVGKWKR CSTTCGEGQ KHRTVECKD TKTQTTLVD QFCDNQPKP KTTTKCEKY MCDYAWITS PWTQCSAFC GQGVQRRNV SCHRVYGRG AVDPKPLMF NKAKPNDFC NLYEKPVEE MGCNGGHCK EVYVWRSDP WKECSYSCG KKGRRSRQI YCMNVKTGQ KVEKRHCPR HSRPKRRLK CNQWRCLFK SCKEIQTHT KTRDNKDYL ITIRNRPAW VYCYKMDTN QPEEYITLH GDTLNYAEN YDKRLKNPH SCPYDGQRF DLCDCLPIE SDRSGFTTF WKVRLNITS LRIIGDDFT FSRQTKGMK VPYGTAGDC YSATEGCAQ ARFSIDLKG TSFQLSENV RWHSIGKYA TARIDTFEK RVVGLCGGY CGNCVPEPS VGLQVEIT
- >TC015139 RA
atgttcgtt tttcagacc ggctacatc cgcacttct cacgacaat tactacatc gagccggcg gagaaatcc tccggggaa tacccctta ggcagtttc cttcaccgg atcaaaaaa atccacaat agtgcaccg accggaaac gaagtcgtc gtgaacgaa tcggaccaa tgcgaaacc aacgacaat gagagcatc gtggaggac gacttcggc tcatcagtc gtggccatc ccttcacac actttgaca aaacgtgcc attgttgac gacatcggt gattccccc tttctttcg cgcaaaatc cgcgacgct gattggccc attaactac gaaacggac caaaagacc aacgaatac ttcgtcaaa gtgctcgtc gttgccgat cgcagtatg atacagtac caccaatct aacgaagat ttaaaacac tacatccta atcctgatg tcacacgcg gccctttta tacaaagac gcaagtatc ggaaactcg atttcgtta tcggtggtc aacatctgg ttactgaac aatactgta ttcaccagt aacaactca caagttatg ttacgcagg ttttgcgag tggcagaaa cggtacgtc acggatcgg aaacatgac atggctttg ctatttacg agagaccca atttgcaag aaaaactcc tcgagttgc gacactttg ggcgttgcc gaagtcggc tcaatgtgc aacccttcc tcgagctgc gccatcgtc aaggagcga ggtttgtcc tcctcctac accatagcg cacgaattg ggccacgtt ctcagtatg ctccacgac gagaccgaa agctgttcc cacttcagt cgaggcccg aaatcggaa aacataatg tcccgaatc ctcaacaac gggacaaaa ccctggttg tggtccgag tgctccaag cacttcctc accgagttc ctcgaatca aacaaagcc aaatgtctg ctcaacgcc ccgaccagc aattacatc tcatcggaa atttccgat ttactacca ggagagaac ttcgaagcc gacaaacaa tgcgaacta gaattcggg attggttac aaactgtgc tcttatcaa gcgtcctgt gccgtctta tggtgcact tctaatgac atctacggg tgcaaatcc aacttgcta ccatgggca gatgggaca tcctgtggt cggaaccgc tggtgccac catgggcag tgtattcct ctggaccgc cagaaacta acaccaata ccaggcggc tggggacct tggcagcca tggagcgac tgcagccgc acgtgcggc ggcggcgtg aagcaaacc cgccgttac tgcgactcg ccggtgccc caaaatggg ggcccgtac tgtacgggc aaaagcatt caatacgtt tcgtgcaat acggagtcg tgcgatgat gacgttccc gattttcgg gaagttcaa tgctcacaa ttcgatggt attaataaa aatttggct aatttgacc aaagatgtg aaatgggtg cccaagtat gggctttcg aagagggag gatctgtgc aaattgttt tgcagaccg gaaaattcc aacgcttat tatccgttg aaggacaaa gttatcgac ggaacgaag tgtggattg aattcgttc gatatttgc gtcaatggg atttgcagg ccgggaggg tgtgataac aagctgaat tccaagatg gatctggac gagtgcggc gtttgcggt ggtgataac agcaaatgc gaggaaatc accggcaat tacaacgtt tcaatgact ggctataac atcgtgcta cgtatcccc aaaggcagc tcaaacatt gatatccgt caaaacgcc aacaacgat actaactat ctagtccta gttgatggc gaaaccggg gaatacgtc cttaatagc aaaaccatg attgcgcct caacaaaaa gacgtaatt tttgccggc atcataatc agctatagt ggctcatcg gctgccatt gaacgcata acaacacca aaaaaccac aaactcacc aaagaccta gtccttggc gtactttca gtcggacac gctctcgca cccgatatc acctacaga tatatcata gaccgcgaa aatgccccc aaatatggt tggcgactc tatcgcaac aaatggtcc aaatgcaac tccatatgt agcggtact caatatatg tccccgatt tgtatcgag ttggccacc aacaaggaa caaccagag tctttttgt ccacaacac gaaaaacaa caccacatg caaaaacaa gactgtaat acgcactgc caacttaga tggagcgat gtgtcacgt ggaccttgc tcagtcaat tgtggacaa ggggttcga agtgtttac caccagtgt gtcaaaatc gacatgact aaagaaaac tacgaccgg taccacgaa gttgtggac cacaaacac tgcaatatt ttggggaaa ccccccacc atggaggtg tgccaaggg aggtgcgaa tctactaga tgggagtac accagctgg tccgagtgt tccacttcc tgtggtggt ggtacccaa cgtcggagt gccaaatgc gtggacaat aacagtcgc acccttgac gattcctac tgtcctggt gaaaaaatc accgagcaa cgctgcaac acccagaaa tgtcccatc tgggagtcg cgcgaatgg accccatgt tccgtcacg tgcggtcgt ggtttccgc acaaaaccg tacttttgt cacatcgat ggtaaaatc ctagaccca tcggcttgc gactcgcgc caaaaacac atcgaagaa gagcaatgt tacgaacgc ccatgtgcc ggatggtcc ataggccca tggggcgaa tgctcgacc acgtgtggc gatggtacc agtaccaga caagtcatt tgccagaat ctggacgac aatcaagtc attcaagac tccttctgt tcgggaatt ctcaagccg aacgagacg aaattttgc aaaattgca gattgtgtt cagtcgttg ccgtaccaa aggcggtac gattacggg aacgatatt ttagcgaat acgattgtg agttacgaa agggggttc aagtgggtg acgggggcg tggagtcag tgctcgaaa agttgcgga acgggggtt agttcacgt atggtggtt tgtagaaat gagttgggg gaggaggat gaaaggtat tgtgccaaa agtgtggtt ccggtcaaa accatcgag tgtaatacg ggcaagtgt cccgcttgg gagtttggg ggctggagt gggtgtgat tttaactgc gagaagcgg agacaagtc acttgtcga gcggcctcc ggcagcttc gtcgaagac acccaatgt gacaaacac cgtaaacca tcaaccacc acaaaatgc aaactctcc caatgtccc cacctagcc tccaaacct tacagcccc catcgttac cgctggtct gtcggtaaa tggaaacgt tgttcaaca acgtgtggc gaaggccaa aaacaccgt actgtcgaa tgtaaagat acgaaaaca caaactacg ctcgttgac caattttgt gacaatcaa cccaaaccg aaaactacc accaaatgc gagaaatac atgtgtgac tatgcatgg attacgagt ccttggacc caatgttcg gccttttgt ggacaagga gtacagagg aggaatgtt agttgtcat cgtgtgtat ggtcggggg gctgttgat ccaaagcct ctgatgttt aataaagcg aaaccgaac gatttttgc aatttgtat gaaaaacca gtggaggag atgggatgt aatgggggg cattgcaag gaggtctac gtgtggagg agcgacccc tggaaggag tgttcgtat tcctgcggt aaaaagggc cgccgcagc cgccagatt tactgcatg aacgtaaaa acgggtcaa aaggtcgag aaacgtcac tgccctcga cattccaga cctaaacgc cgcctcaaa tgtaaccaa tggagatgt ttattcaaa agttgcaaa gaaattcaa acacacacc aaaacaaga gacaacaaa gactacttg attacaatc cgaaatcgg ccagcttgg gtctactgt tacaaaatg gacaccaac caaccagag gaatacata accttgcac ggtgatact ctcaattat gccgaaaat tacgacaaa cgattgaaa aatccgcac tcgtgccct tacgatggg caacgtttc gacctttgt gactgcttg ccaatcgag tcggaccgt tccggcttc actacattc tggaaagtg cgtctcaac atcacgtct ctgcgaatt atcggtgac gattttacg ttttcgcga caaacgaaa ggcatgaaa gtgccttat ggcacagct ggcgattgt tatagtgcc acagaaggc tgtgcccaa gcccgattt tccatagat ttgaaagga acttcgttt caactttcg gaaaacgtg cgatggcac agtatcgga aaatatgcc actgccagg attgacact tttgagaaa agggttgtg ggcctgtgt gggggctat tgcgggaat tgcgttcct gagcccagt gtgggtctc caagtcgag atcacgtag
- >TC015139 RA
ccgtaaatc accgccaat tatgttcgt ttttcagac cggctacat ccgcacttc tcacgacaa ttactacat cgagccggc ggagaaatc ctccgggga atacccctt aggcagttt ccttcaccg gatcaaaaa aatccacaa tagtgcacc gaccggaaa cgaagtcgt cgtgaacga atcggacca atgcgaaac caacgacaa tgagagcat cgtggagga cgacttcgg ctcatcagt cgtggccat cccttcaca cactttgac aaaacgtgc cattgttga cgacatcgg tgattcccc ctttctttc gcgcaaaat ccgcgacgc tgattggcc cattaacta cgaaacgga ccaaaagac caacgaata cttcgtcaa agtgctcgt cgttgccga tcgcagtat gatacagta ccaccaatc taacgaaga tttaaaaca ctacatcct aatcctgat gtcacacgc ggccctttt atacaaaga cgcaagtat cggaaactc gatttcgtt atcggtggt caacatctg gttactgaa caatactgt attcaccag taacaactc acaagttat gttacgcag gttttgcga gtggcagaa acggtacgt cacggatcg gaaacatga catggcttt gctatttac gagagaccc aatttgcaa gaaaaactc ctcgagttg cgacacttt gggcgttgc cgaagtcgg ctcaatgtg caacccttc ctcgagctg cgccatcgt caaggagcg aggtttgtc ctcctccta caccatagc gcacgaatt gggccacgt tctcagtat gctccacga cgagaccga aagctgttc ccacttcag tcgaggccc gaaatcgga aaacataat gtcccgaat cctcaacaa cgggacaaa accctggtt gtggtccga gtgctccaa gcacttcct caccgagtt cctcgaatc aaacaaagc caaatgtct gctcaacgc cccgaccag caattacat ctcatcgga aatttccga tttactacc aggagagaa cttcgaagc cgacaaaca atgcgaact agaattcgg gattggtta caaactgtg ctcttatca agcgtcctg tgccgtctt atggtgcac ttctaatga catctacgg gtgcaaatc caacttgct accatgggc agatgggac atcctgtgg tcggaaccg ctggtgcca ccatgggca gtgtattcc tctggaccg ccagaaact aacaccaat accaggcgg ctggggacc ttggcagcc atggagcga ctgcagccg cacgtgcgg cggcggcgt gaagcaaac ccgccgtta ctgcgactc gccggtgcc ccaaaatgg gggcccgta ctgtacggg caaaagcat tcaatacgt ttcgtgcaa tacggagtc gtgcgatga tgacgttcc cgattttcg ggaagttca atgctcaca attcgatgg tattaataa aaatttggc taatttgac caaagatgt gaaatgggt gcccaagta tgggctttc gaagaggga ggatctgtg caaattgtt ttgcagacc ggaaaattc caacgctta ttatccgtt gaaggacaa agttatcga cggaacgaa gtgtggatt gaattcgtt cgatatttg cgtcaatgg gatttgcag gccgggagg gtgtgataa caagctgaa ttccaagat ggatctgga cgagtgcgg cgtttgcgg tggtgataa cagcaaatg cgaggaaat caccggcaa ttacaacgt ttcaatgac tggctataa catcgtgct acgtatccc caaaggcag ctcaaacat tgatatccg tcaaaacgc caacaacga tactaacta tctagtcct agttgatgg cgaaaccgg ggaatacgt ccttaatag caaaaccat gattgcgcc tcaacaaaa agacgtaat ttttgccgg catcataat cagctatag tggctcatc ggctgccat tgaacgcat aacaacacc aaaaaacca caaactcac caaagacct agtccttgg cgtactttc agtcggaca cgctctcgc acccgatat cacctacag atatatcat agaccgcga aaatgcccc caaatatgg ttggcgact ctatcgcaa caaatggtc caaatgcaa ctccatatg tagcggtac tcaatatat gtccccgat ttgtatcga gttggccac caacaagga acaaccaga gtctttttg tccacaaca cgaaaaaca acaccacat gcaaaaaca agactgtaa tacgcactg ccaacttag atggagcga tgtgtcacg tggaccttg ctcagtcaa ttgtggaca aggggttcg aagtgttta ccaccagtg tgtcaaaat cgacatgac taaagaaaa ctacgaccg gtaccacga agttgtgga ccacaaaca ctgcaatat tttggggaa accccccac catggaggt gtgccaagg gaggtgcga atctactag atgggagta caccagctg gtccgagtg ttccacttc ctgtggtgg tggtaccca acgtcggag tgccaaatg cgtggacaa taacagtcg cacccttga cgattccta ctgtcctgg tgaaaaaat caccgagca acgctgcaa cacccagaa atgtcccat ctgggagtc gcgcgaatg gaccccatg ttccgtcac gtgcggtcg tggtttccg cacaaaacc gtacttttg tcacatcga tggtaaaat cctagaccc atcggcttg cgactcgcg ccaaaaaca catcgaaga agagcaatg ttacgaacg cccatgtgc cggatggtc cataggccc atggggcga atgctcgac cacgtgtgg cgatggtac cagtaccag acaagtcat ttgccagaa tctggacga caatcaagt cattcaaga ctccttctg ttcgggaat tctcaagcc gaacgagac gaaattttg caaaattgc agattgtgt tcagtcgtt gccgtacca aaggcggta cgattacgg gaacgatat tttagcgaa tacgattgt gagttacga aagggggtt caagtgggt gacgggggc gtggagtca gtgctcgaa aagttgcgg aacgggggt tagttcacg tatggtggt ttgtagaaa tgagttggg ggaggagga tgaaaggta ttgtgccaa aagtgtggt tccggtcaa aaccatcga gtgtaatac gggcaagtg tcccgcttg ggagtttgg gggctggag tgggtgtga ttttaactg cgagaagcg gagacaagt cacttgtcg agcggcctc cggcagctt cgtcgaaga cacccaatg tgacaaaca ccgtaaacc atcaaccac cacaaaatg caaactctc ccaatgtcc ccacctagc ctccaaacc ttacagccc ccatcgtta ccgctggtc tgtcggtaa atggaaacg ttgttcaac aacgtgtgg cgaaggcca aaaacaccg tactgtcga atgtaaaga tacgaaaac acaaactac gctcgttga ccaattttg tgacaatca acccaaacc gaaaactac caccaaatg cgagaaata catgtgtga ctatgcatg gattacgag tccttggac ccaatgttc ggccttttg tggacaagg agtacagag gaggaatgt tagttgtca tcgtgtgta tggtcgggg ggctgttga tccaaagcc tctgatgtt taataaagc gaaaccgaa cgatttttg caatttgta tgaaaaacc agtggagga gatgggatg taatggggg gcattgcaa ggaggtcta cgtgtggag gagcgaccc ctggaagga gtgttcgta ttcctgcgg taaaaaggg ccgccgcag ccgccagat ttactgcat gaacgtaaa aacgggtca aaaggtcga gaaacgtca ctgccctcg acattccag acctaaacg ccgcctcaa atgtaacca atggagatg tttattcaa aagttgcaa agaaattca aacacacac caaaacaag agacaacaa agactactt gattacaat ccgaaatcg gccagcttg ggtctactg ttacaaaat ggacaccaa ccaaccaga ggaatacat aaccttgca cggtgatac tctcaatta tgccgaaaa ttacgacaa acgattgaa aaatccgca ctcgtgccc ttacgatgg gcaacgttt cgacctttg tgactgctt gccaatcga gtcggaccg ttccggctt cactacatt ctggaaagt gcgtctcaa catcacgtc tctgcgaat tatcggtga cgattttac gttttcgcg acaaacgaa aggcatgaa agtgcctta tggcacagc tggcgattg ttatagtgc cacagaagg ctgtgccca agcccgatt ttccataga tttgaaagg aacttcgtt tcaactttc ggaaaacgt gcgatggca cagtatcgg aaaatatgc cactgccag gattgacac ttttgagaa aagggttgt gggcctgtg tgggggcta ttgcgggaa ttgcgttcc tgagcccag tgtgggtct ccaagtcga gatcacgta gtctggtat tacaaacta atcagtatt ttagcctta tgtactgcc atataatta tggaaactg ccaaatact gcctttttt acctcacat tgagggagt ttttttgta ttatatagt tatgctgcc attcgtact tttttatac acttataat accctcgtg acgtgcctt ttttgtata tatttttaa ctaatttat tgttaagtg ccgtgtaaa ttgtattta ttgttaatg tttatggaa aataaaata acacgtatt ga
