Status
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Gene information
General information
TC015139 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2447774..2461185
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015139
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08518

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    tropharium structure irregular - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber potentially position anteriorized - Number of animals showing the phenotype: 4

    vitellarium size decreased - Number of animals showing the phenotype: 4
    tropharium size decreased - Number of animals showing the phenotype: 4
    tropharium circumference increased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015139 RA
      MFVFQTGYI RTSHDNYYI EPAEKSSGE YPLGSFLHR 
      IKKIHNSAP TGNEVVVNE SDQCETNDN ESIVEDDFG 
      SSVVAIPSH TLTKRAIVD DIGDSPFLS RKIRDADWP 
      INYETDQKT NEYFVKVLV VADRSMIQY HQSNEDLKH 
      YILILMSHA ALLYKDASI GNSISLSVV NIWLLNNTV 
      FTSNNSQVM LRRFCEWQK RYVTDRKHD MALLFTRDP 
      ICKKNSSSC DTLGVAEVG SMCNPSSSC AIVKERGLS 
      SSYTIAHEL GHVLSMLHD ETESCSHFS RGPKSENIM 
      SRILNNGTK PWLWSECSK HFLTEFLES NKAKCLLNA 
      PTSNYISSE ISDLLPGEN FEADKQCEL EFGIGYKLC 
      SYQASCAVL WCTSNDIYG CKSNLLPWA DGTSCGRNR 
      WCHHGQCIP LDRQKLTPI PGGWGPWQP WSDCSRTCG 
      GGVKQTRRY CDSPVPQNG GPYCTGKSI QYVSCNTES 
      CDDDVPDFR EVQCSQFDG INKNLANLT KDVKWVPKY 
      GLSKREDLC KLFCRPENS NAYYPLKDK VIDGTKCGL 
      NSFDICVNG ICRPGGCDN KLNSKMDLD ECGVCGGDN 
      SKCEEITGN YNVSMTGYN IVLRIPKGS SNIDIRQNA 
      NNDTNYLVL VDGETGEYV LNSKTMIAP QQKDVIFAG 
      IIISYSGSS AAIERITTP KNHKLTKDL VLGVLSVGH 
      ALAPDITYR YIIDRENAP KYGWRLYRN KWSKCNSIC 
      SGTQYMSPI CIELATNKE QPESFCPQH EKQHHMQKQ 
      DCNTHCQLR WSDVSRGPC SVNCGQGVR SVYHQCVKI 
      DMTKENYDR YHEVVDHKH CNILGKPPT MEVCQGRCE 
      STRWEYTSW SECSTSCGG GTQRRSAKC VDNNSRTLD 
      DSYCPGEKI TEQRCNTQK CPIWESREW TPCSVTCGR 
      GFRTKPYFC HIDGKILDP SACDSRQKH IEEEQCYER 
      PCAGWSIGP WGECSTTCG DGTSTRQVI CQNLDDNQV 
      IQDSFCSGI LKPNETKFC KIADCVQSL PYQRRYDYG 
      NDILANTIV SYERGFKWV TGAWSQCSK SCGTGVSSR 
      MVVCRNELG EEDERYCAK SVVPVKTIE CNTGKCPAW 
      EFGGWSGCD FNCEKRRQV TCRAASGSF VEDTQCDKH 
      RKPSTTTKC KLSQCPHLA SKPYSPHRY RWSVGKWKR 
      CSTTCGEGQ KHRTVECKD TKTQTTLVD QFCDNQPKP 
      KTTTKCEKY MCDYAWITS PWTQCSAFC GQGVQRRNV 
      SCHRVYGRG AVDPKPLMF NKAKPNDFC NLYEKPVEE 
      MGCNGGHCK EVYVWRSDP WKECSYSCG KKGRRSRQI 
      YCMNVKTGQ KVEKRHCPR HSRPKRRLK CNQWRCLFK 
      SCKEIQTHT KTRDNKDYL ITIRNRPAW VYCYKMDTN 
      QPEEYITLH GDTLNYAEN YDKRLKNPH SCPYDGQRF 
      DLCDCLPIE SDRSGFTTF WKVRLNITS LRIIGDDFT 
      FSRQTKGMK VPYGTAGDC YSATEGCAQ ARFSIDLKG 
      TSFQLSENV RWHSIGKYA TARIDTFEK RVVGLCGGY 
      CGNCVPEPS VGLQVEIT
    CDS sequence
    • >TC015139 RA
      atgttcgtt tttcagacc ggctacatc cgcacttct 
      cacgacaat tactacatc gagccggcg gagaaatcc 
      tccggggaa tacccctta ggcagtttc cttcaccgg 
      atcaaaaaa atccacaat agtgcaccg accggaaac 
      gaagtcgtc gtgaacgaa tcggaccaa tgcgaaacc 
      aacgacaat gagagcatc gtggaggac gacttcggc 
      tcatcagtc gtggccatc ccttcacac actttgaca 
      aaacgtgcc attgttgac gacatcggt gattccccc 
      tttctttcg cgcaaaatc cgcgacgct gattggccc 
      attaactac gaaacggac caaaagacc aacgaatac 
      ttcgtcaaa gtgctcgtc gttgccgat cgcagtatg 
      atacagtac caccaatct aacgaagat ttaaaacac 
      tacatccta atcctgatg tcacacgcg gccctttta 
      tacaaagac gcaagtatc ggaaactcg atttcgtta 
      tcggtggtc aacatctgg ttactgaac aatactgta 
      ttcaccagt aacaactca caagttatg ttacgcagg 
      ttttgcgag tggcagaaa cggtacgtc acggatcgg 
      aaacatgac atggctttg ctatttacg agagaccca 
      atttgcaag aaaaactcc tcgagttgc gacactttg 
      ggcgttgcc gaagtcggc tcaatgtgc aacccttcc 
      tcgagctgc gccatcgtc aaggagcga ggtttgtcc 
      tcctcctac accatagcg cacgaattg ggccacgtt 
      ctcagtatg ctccacgac gagaccgaa agctgttcc 
      cacttcagt cgaggcccg aaatcggaa aacataatg 
      tcccgaatc ctcaacaac gggacaaaa ccctggttg 
      tggtccgag tgctccaag cacttcctc accgagttc 
      ctcgaatca aacaaagcc aaatgtctg ctcaacgcc 
      ccgaccagc aattacatc tcatcggaa atttccgat 
      ttactacca ggagagaac ttcgaagcc gacaaacaa 
      tgcgaacta gaattcggg attggttac aaactgtgc 
      tcttatcaa gcgtcctgt gccgtctta tggtgcact 
      tctaatgac atctacggg tgcaaatcc aacttgcta 
      ccatgggca gatgggaca tcctgtggt cggaaccgc 
      tggtgccac catgggcag tgtattcct ctggaccgc 
      cagaaacta acaccaata ccaggcggc tggggacct 
      tggcagcca tggagcgac tgcagccgc acgtgcggc 
      ggcggcgtg aagcaaacc cgccgttac tgcgactcg 
      ccggtgccc caaaatggg ggcccgtac tgtacgggc 
      aaaagcatt caatacgtt tcgtgcaat acggagtcg 
      tgcgatgat gacgttccc gattttcgg gaagttcaa 
      tgctcacaa ttcgatggt attaataaa aatttggct 
      aatttgacc aaagatgtg aaatgggtg cccaagtat 
      gggctttcg aagagggag gatctgtgc aaattgttt 
      tgcagaccg gaaaattcc aacgcttat tatccgttg 
      aaggacaaa gttatcgac ggaacgaag tgtggattg 
      aattcgttc gatatttgc gtcaatggg atttgcagg 
      ccgggaggg tgtgataac aagctgaat tccaagatg 
      gatctggac gagtgcggc gtttgcggt ggtgataac 
      agcaaatgc gaggaaatc accggcaat tacaacgtt 
      tcaatgact ggctataac atcgtgcta cgtatcccc 
      aaaggcagc tcaaacatt gatatccgt caaaacgcc 
      aacaacgat actaactat ctagtccta gttgatggc 
      gaaaccggg gaatacgtc cttaatagc aaaaccatg 
      attgcgcct caacaaaaa gacgtaatt tttgccggc 
      atcataatc agctatagt ggctcatcg gctgccatt 
      gaacgcata acaacacca aaaaaccac aaactcacc 
      aaagaccta gtccttggc gtactttca gtcggacac 
      gctctcgca cccgatatc acctacaga tatatcata 
      gaccgcgaa aatgccccc aaatatggt tggcgactc 
      tatcgcaac aaatggtcc aaatgcaac tccatatgt 
      agcggtact caatatatg tccccgatt tgtatcgag 
      ttggccacc aacaaggaa caaccagag tctttttgt 
      ccacaacac gaaaaacaa caccacatg caaaaacaa 
      gactgtaat acgcactgc caacttaga tggagcgat 
      gtgtcacgt ggaccttgc tcagtcaat tgtggacaa 
      ggggttcga agtgtttac caccagtgt gtcaaaatc 
      gacatgact aaagaaaac tacgaccgg taccacgaa 
      gttgtggac cacaaacac tgcaatatt ttggggaaa 
      ccccccacc atggaggtg tgccaaggg aggtgcgaa 
      tctactaga tgggagtac accagctgg tccgagtgt 
      tccacttcc tgtggtggt ggtacccaa cgtcggagt 
      gccaaatgc gtggacaat aacagtcgc acccttgac 
      gattcctac tgtcctggt gaaaaaatc accgagcaa 
      cgctgcaac acccagaaa tgtcccatc tgggagtcg 
      cgcgaatgg accccatgt tccgtcacg tgcggtcgt 
      ggtttccgc acaaaaccg tacttttgt cacatcgat 
      ggtaaaatc ctagaccca tcggcttgc gactcgcgc 
      caaaaacac atcgaagaa gagcaatgt tacgaacgc 
      ccatgtgcc ggatggtcc ataggccca tggggcgaa 
      tgctcgacc acgtgtggc gatggtacc agtaccaga 
      caagtcatt tgccagaat ctggacgac aatcaagtc 
      attcaagac tccttctgt tcgggaatt ctcaagccg 
      aacgagacg aaattttgc aaaattgca gattgtgtt 
      cagtcgttg ccgtaccaa aggcggtac gattacggg 
      aacgatatt ttagcgaat acgattgtg agttacgaa 
      agggggttc aagtgggtg acgggggcg tggagtcag 
      tgctcgaaa agttgcgga acgggggtt agttcacgt 
      atggtggtt tgtagaaat gagttgggg gaggaggat 
      gaaaggtat tgtgccaaa agtgtggtt ccggtcaaa 
      accatcgag tgtaatacg ggcaagtgt cccgcttgg 
      gagtttggg ggctggagt gggtgtgat tttaactgc 
      gagaagcgg agacaagtc acttgtcga gcggcctcc 
      ggcagcttc gtcgaagac acccaatgt gacaaacac 
      cgtaaacca tcaaccacc acaaaatgc aaactctcc 
      caatgtccc cacctagcc tccaaacct tacagcccc 
      catcgttac cgctggtct gtcggtaaa tggaaacgt 
      tgttcaaca acgtgtggc gaaggccaa aaacaccgt 
      actgtcgaa tgtaaagat acgaaaaca caaactacg 
      ctcgttgac caattttgt gacaatcaa cccaaaccg 
      aaaactacc accaaatgc gagaaatac atgtgtgac 
      tatgcatgg attacgagt ccttggacc caatgttcg 
      gccttttgt ggacaagga gtacagagg aggaatgtt 
      agttgtcat cgtgtgtat ggtcggggg gctgttgat 
      ccaaagcct ctgatgttt aataaagcg aaaccgaac 
      gatttttgc aatttgtat gaaaaacca gtggaggag 
      atgggatgt aatgggggg cattgcaag gaggtctac 
      gtgtggagg agcgacccc tggaaggag tgttcgtat 
      tcctgcggt aaaaagggc cgccgcagc cgccagatt 
      tactgcatg aacgtaaaa acgggtcaa aaggtcgag 
      aaacgtcac tgccctcga cattccaga cctaaacgc 
      cgcctcaaa tgtaaccaa tggagatgt ttattcaaa 
      agttgcaaa gaaattcaa acacacacc aaaacaaga 
      gacaacaaa gactacttg attacaatc cgaaatcgg 
      ccagcttgg gtctactgt tacaaaatg gacaccaac 
      caaccagag gaatacata accttgcac ggtgatact 
      ctcaattat gccgaaaat tacgacaaa cgattgaaa 
      aatccgcac tcgtgccct tacgatggg caacgtttc 
      gacctttgt gactgcttg ccaatcgag tcggaccgt 
      tccggcttc actacattc tggaaagtg cgtctcaac 
      atcacgtct ctgcgaatt atcggtgac gattttacg 
      ttttcgcga caaacgaaa ggcatgaaa gtgccttat 
      ggcacagct ggcgattgt tatagtgcc acagaaggc 
      tgtgcccaa gcccgattt tccatagat ttgaaagga 
      acttcgttt caactttcg gaaaacgtg cgatggcac 
      agtatcgga aaatatgcc actgccagg attgacact 
      tttgagaaa agggttgtg ggcctgtgt gggggctat 
      tgcgggaat tgcgttcct gagcccagt gtgggtctc 
      caagtcgag atcacgtag
    mRNA sequence
    • >TC015139 RA
      ccgtaaatc accgccaat tatgttcgt ttttcagac 
      cggctacat ccgcacttc tcacgacaa ttactacat 
      cgagccggc ggagaaatc ctccgggga atacccctt 
      aggcagttt ccttcaccg gatcaaaaa aatccacaa 
      tagtgcacc gaccggaaa cgaagtcgt cgtgaacga 
      atcggacca atgcgaaac caacgacaa tgagagcat 
      cgtggagga cgacttcgg ctcatcagt cgtggccat 
      cccttcaca cactttgac aaaacgtgc cattgttga 
      cgacatcgg tgattcccc ctttctttc gcgcaaaat 
      ccgcgacgc tgattggcc cattaacta cgaaacgga 
      ccaaaagac caacgaata cttcgtcaa agtgctcgt 
      cgttgccga tcgcagtat gatacagta ccaccaatc 
      taacgaaga tttaaaaca ctacatcct aatcctgat 
      gtcacacgc ggccctttt atacaaaga cgcaagtat 
      cggaaactc gatttcgtt atcggtggt caacatctg 
      gttactgaa caatactgt attcaccag taacaactc 
      acaagttat gttacgcag gttttgcga gtggcagaa 
      acggtacgt cacggatcg gaaacatga catggcttt 
      gctatttac gagagaccc aatttgcaa gaaaaactc 
      ctcgagttg cgacacttt gggcgttgc cgaagtcgg 
      ctcaatgtg caacccttc ctcgagctg cgccatcgt 
      caaggagcg aggtttgtc ctcctccta caccatagc 
      gcacgaatt gggccacgt tctcagtat gctccacga 
      cgagaccga aagctgttc ccacttcag tcgaggccc 
      gaaatcgga aaacataat gtcccgaat cctcaacaa 
      cgggacaaa accctggtt gtggtccga gtgctccaa 
      gcacttcct caccgagtt cctcgaatc aaacaaagc 
      caaatgtct gctcaacgc cccgaccag caattacat 
      ctcatcgga aatttccga tttactacc aggagagaa 
      cttcgaagc cgacaaaca atgcgaact agaattcgg 
      gattggtta caaactgtg ctcttatca agcgtcctg 
      tgccgtctt atggtgcac ttctaatga catctacgg 
      gtgcaaatc caacttgct accatgggc agatgggac 
      atcctgtgg tcggaaccg ctggtgcca ccatgggca 
      gtgtattcc tctggaccg ccagaaact aacaccaat 
      accaggcgg ctggggacc ttggcagcc atggagcga 
      ctgcagccg cacgtgcgg cggcggcgt gaagcaaac 
      ccgccgtta ctgcgactc gccggtgcc ccaaaatgg 
      gggcccgta ctgtacggg caaaagcat tcaatacgt 
      ttcgtgcaa tacggagtc gtgcgatga tgacgttcc 
      cgattttcg ggaagttca atgctcaca attcgatgg 
      tattaataa aaatttggc taatttgac caaagatgt 
      gaaatgggt gcccaagta tgggctttc gaagaggga 
      ggatctgtg caaattgtt ttgcagacc ggaaaattc 
      caacgctta ttatccgtt gaaggacaa agttatcga 
      cggaacgaa gtgtggatt gaattcgtt cgatatttg 
      cgtcaatgg gatttgcag gccgggagg gtgtgataa 
      caagctgaa ttccaagat ggatctgga cgagtgcgg 
      cgtttgcgg tggtgataa cagcaaatg cgaggaaat 
      caccggcaa ttacaacgt ttcaatgac tggctataa 
      catcgtgct acgtatccc caaaggcag ctcaaacat 
      tgatatccg tcaaaacgc caacaacga tactaacta 
      tctagtcct agttgatgg cgaaaccgg ggaatacgt 
      ccttaatag caaaaccat gattgcgcc tcaacaaaa 
      agacgtaat ttttgccgg catcataat cagctatag 
      tggctcatc ggctgccat tgaacgcat aacaacacc 
      aaaaaacca caaactcac caaagacct agtccttgg 
      cgtactttc agtcggaca cgctctcgc acccgatat 
      cacctacag atatatcat agaccgcga aaatgcccc 
      caaatatgg ttggcgact ctatcgcaa caaatggtc 
      caaatgcaa ctccatatg tagcggtac tcaatatat 
      gtccccgat ttgtatcga gttggccac caacaagga 
      acaaccaga gtctttttg tccacaaca cgaaaaaca 
      acaccacat gcaaaaaca agactgtaa tacgcactg 
      ccaacttag atggagcga tgtgtcacg tggaccttg 
      ctcagtcaa ttgtggaca aggggttcg aagtgttta 
      ccaccagtg tgtcaaaat cgacatgac taaagaaaa 
      ctacgaccg gtaccacga agttgtgga ccacaaaca 
      ctgcaatat tttggggaa accccccac catggaggt 
      gtgccaagg gaggtgcga atctactag atgggagta 
      caccagctg gtccgagtg ttccacttc ctgtggtgg 
      tggtaccca acgtcggag tgccaaatg cgtggacaa 
      taacagtcg cacccttga cgattccta ctgtcctgg 
      tgaaaaaat caccgagca acgctgcaa cacccagaa 
      atgtcccat ctgggagtc gcgcgaatg gaccccatg 
      ttccgtcac gtgcggtcg tggtttccg cacaaaacc 
      gtacttttg tcacatcga tggtaaaat cctagaccc 
      atcggcttg cgactcgcg ccaaaaaca catcgaaga 
      agagcaatg ttacgaacg cccatgtgc cggatggtc 
      cataggccc atggggcga atgctcgac cacgtgtgg 
      cgatggtac cagtaccag acaagtcat ttgccagaa 
      tctggacga caatcaagt cattcaaga ctccttctg 
      ttcgggaat tctcaagcc gaacgagac gaaattttg 
      caaaattgc agattgtgt tcagtcgtt gccgtacca 
      aaggcggta cgattacgg gaacgatat tttagcgaa 
      tacgattgt gagttacga aagggggtt caagtgggt 
      gacgggggc gtggagtca gtgctcgaa aagttgcgg 
      aacgggggt tagttcacg tatggtggt ttgtagaaa 
      tgagttggg ggaggagga tgaaaggta ttgtgccaa 
      aagtgtggt tccggtcaa aaccatcga gtgtaatac 
      gggcaagtg tcccgcttg ggagtttgg gggctggag 
      tgggtgtga ttttaactg cgagaagcg gagacaagt 
      cacttgtcg agcggcctc cggcagctt cgtcgaaga 
      cacccaatg tgacaaaca ccgtaaacc atcaaccac 
      cacaaaatg caaactctc ccaatgtcc ccacctagc 
      ctccaaacc ttacagccc ccatcgtta ccgctggtc 
      tgtcggtaa atggaaacg ttgttcaac aacgtgtgg 
      cgaaggcca aaaacaccg tactgtcga atgtaaaga 
      tacgaaaac acaaactac gctcgttga ccaattttg 
      tgacaatca acccaaacc gaaaactac caccaaatg 
      cgagaaata catgtgtga ctatgcatg gattacgag 
      tccttggac ccaatgttc ggccttttg tggacaagg 
      agtacagag gaggaatgt tagttgtca tcgtgtgta 
      tggtcgggg ggctgttga tccaaagcc tctgatgtt 
      taataaagc gaaaccgaa cgatttttg caatttgta 
      tgaaaaacc agtggagga gatgggatg taatggggg 
      gcattgcaa ggaggtcta cgtgtggag gagcgaccc 
      ctggaagga gtgttcgta ttcctgcgg taaaaaggg 
      ccgccgcag ccgccagat ttactgcat gaacgtaaa 
      aacgggtca aaaggtcga gaaacgtca ctgccctcg 
      acattccag acctaaacg ccgcctcaa atgtaacca 
      atggagatg tttattcaa aagttgcaa agaaattca 
      aacacacac caaaacaag agacaacaa agactactt 
      gattacaat ccgaaatcg gccagcttg ggtctactg 
      ttacaaaat ggacaccaa ccaaccaga ggaatacat 
      aaccttgca cggtgatac tctcaatta tgccgaaaa 
      ttacgacaa acgattgaa aaatccgca ctcgtgccc 
      ttacgatgg gcaacgttt cgacctttg tgactgctt 
      gccaatcga gtcggaccg ttccggctt cactacatt 
      ctggaaagt gcgtctcaa catcacgtc tctgcgaat 
      tatcggtga cgattttac gttttcgcg acaaacgaa 
      aggcatgaa agtgcctta tggcacagc tggcgattg 
      ttatagtgc cacagaagg ctgtgccca agcccgatt 
      ttccataga tttgaaagg aacttcgtt tcaactttc 
      ggaaaacgt gcgatggca cagtatcgg aaaatatgc 
      cactgccag gattgacac ttttgagaa aagggttgt 
      gggcctgtg tgggggcta ttgcgggaa ttgcgttcc 
      tgagcccag tgtgggtct ccaagtcga gatcacgta 
      gtctggtat tacaaacta atcagtatt ttagcctta 
      tgtactgcc atataatta tggaaactg ccaaatact 
      gcctttttt acctcacat tgagggagt ttttttgta 
      ttatatagt tatgctgcc attcgtact tttttatac 
      acttataat accctcgtg acgtgcctt ttttgtata 
      tatttttaa ctaatttat tgttaagtg ccgtgtaaa 
      ttgtattta ttgttaatg tttatggaa aataaaata 
      acacgtatt ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY