Status
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Gene information
General information
TC033365
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015551,TC015550
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2199388..2206145
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033365
    iBeetle screen iB_08509

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head musculature specific subset potentially size increased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    gena triplet bristle number increased - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna antennal flagellum sometimes not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna pedicellus size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033365 RA
      MLFSAIILI LALNYFYYK WKFTYWQCK KIPTLAPKI 
      PFGDLPNPL KQAEATGVH IKRLYDQIK GKGWKHAGL 
      YFLARPVYM VVDLEYVKN ILTKDFHHF VDRGFYFNE 
      KDDPISAHV FAVGGQKWR NLRTKLTPT FTSGKMKMM 
      FQTLLECEA NLLKRFEKE HKSAIDIKD ILGCFTTDI 
      IGSCGFGLD FNTFEDENS PFRKYGKKV FASNLTRRL 
      QIFFFTHFP SMARALKVR HFSKDVADF FTKVVKDTV 
      EFRERNNYK RKDFMQLLI DLKNCNSGL TLNEITAQS 
      LIFFLAGFE TSSTTMTFA LYELAKNEK IQERVRDEI 
      FSILGEEKI TYEALQKMK YLALVINET LRKYPPLSF 
      LTRECVQDY KIPDQDVII EKGTKVVIS ILGMHRDQE 
      FYPDPETFD PGRFSEGNV VSRHQYAYI PFGEGPRIC 
      MGLRFGMMQ TKVGLVSLL RKFKFTVNG RTKEPIKFD 
      VRGFILAAE GDIWLNAQK LHSKMVLSM DRWVGKVAI 
      VTGASSGIG AAIADALVS KGLIVAGLA RRTDLIENR 
      AKQLKNGKL YAVKADMTV EKDILDAFK WVSENLGPV 
      HVLINNAGF AKEGLLTEG ETEIWRKIL EINVLGLCI 
      ATREAVKIM KTNGINGHV IHINSILGH SINMLKVNV 
      YPATKFGVT ALTETLRQE LISLESKIK VTSISPGLV 
      ATEMTSLRK DLSEERKKL FGSRPLLNP EDIADGVVY 
      ALSTPEHVQ VHELTIKPL GEPTA
    CDS sequence
    • >TC033365 RA
      atgcttttc agtgcaata atcctaatc ctagccttg 
      aattatttc tattacaaa tggaaattt acttactgg 
      caatgcaaa aaaatccca actttggcc ccaaaaatc 
      cccttcggc gacctcccc aaccctttg aaacaagcg 
      gaagccacg ggtgttcac atcaaacgt ctctatgac 
      caaatcaaa ggcaaaggc tggaaacac gccggtttg 
      tattttttg gcacgtcct gtatacatg gtggtcgac 
      ttagagtac gtgaaaaat atcctaact aaagatttc 
      caccatttc gtcgatcgg ggattttat ttcaacgaa 
      aaggacgat ccgataagt gcccacgtt ttcgcagtc 
      ggcggccaa aaatggcga aacttgaga actaaacta 
      accccaact ttcacctcc gggaaaatg aaaatgatg 
      ttccagacg ttactagaa tgcgaagct aatttgcta 
      aaacgcttc gaaaaggaa cacaaaagt gcgatcgat 
      attaaagat attttgggc tgttttaca actgatata 
      atcggttct tgtggtttt ggcctcgat tttaacact 
      tttgaggac gaaaattcg cccttccgt aaatacggg 
      aaaaaagtt ttcgcctca aatctcaca agaaggctc 
      caaattttt ttcttcact catttcccg tcaatggca 
      cgggcccta aaagtgcgc cacttttcc aaagacgtg 
      gccgatttt ttcacaaaa gtggtgaaa gacacggtc 
      gagttccgg gagagaaat aattacaag cgcaaggat 
      tttatgcaa cttttgatt gatttgaaa aattgcaac 
      tctggactg accttgaac gaaattacg gcccaaagt 
      ttgattttc tttttggcg ggttttgaa acctcgtcc 
      actaccatg acttttgca ctttacgaa cttgccaaa 
      aacgagaaa atacaggag cgggtgaga gacgagatt 
      ttttcaatt ctgggtgaa gaaaaaatc acgtatgaa 
      gcgctccaa aaaatgaaa tacttggca cttgttata 
      aatgaaact ttaaggaag tacccgcca ctgtctttc 
      ctgacgcgg gaatgtgtc caggattat aaaatcccg 
      gaccaggat gtgatcata gaaaaggga acaaaagtc 
      gtaatttca attttgggg atgcacagg gaccaggaa 
      ttttatcct gatcctgag acattcgat cctgggcgt 
      ttttcggaa ggtaatgtt gtctcaaga caccagtat 
      gcctatatt ccctttggg gaaggtccc cgaatttgt 
      atgggttta aggttcgga atgatgcaa actaaagtc 
      gggttggtt tcgttgttg cgaaaattc aagtttaca 
      gtgaatggg aggacgaaa gaaccaatc aagtttgat 
      gtgaggggt tttattttg gctgcggag ggcgatatt 
      tggctcaat gcccaaaaa cttcactca aaaatggtt 
      ctctccatg gacagatgg gtgggcaaa gtggccatc 
      gtcacgggc gccagctct ggaatcggc gccgccatc 
      gccgacgct ttagtctcc aaaggcctc attgttgct 
      ggccttgcc cgccgcaca gacctgatc gaaaatcgg 
      gccaaacag ctcaaaaac ggcaaactg tacgctgtt 
      aaagccgac atgaccgtg gaaaaagac attctggac 
      gctttcaaa tgggtttcg gaaaatttg ggcccggtc 
      catgtttta ataaacaat gccgggttt gccaaagag 
      gggcttctg accgagggc gagacggaa atctggcgg 
      aaaatcctc gaaattaac gtcctgggt ctgtgtatt 
      gccacaagg gaggctgtg aaaatcatg aaaactaat 
      gggataaac gggcatgtt atccacatt aatagcatt 
      ctggggcat tcgattaat atgttgaaa gttaacgtg 
      tatccagcg acgaaattt ggcgttacg gcccttact 
      gagaccttg aggcaggag ttgataagt cttgagagc 
      aaaattaaa gtcacaagt attagtccg ggtttggtt 
      gccactgaa atgacttct ctgaggaaa gatttgagt 
      gaggaaagg aaaaaattg tttgggagc cgaccgttg 
      ctgaaccct gaagatatc gccgatggg gtggtttat 
      gccctttcg acccctgag catgttcaa gtgcacgaa 
      ctgaccatt aaaccacta ggggaaccc accgcctaa
    mRNA sequence
    • >TC033365 RA
      gaaaaaata gatgctttt cagtgcaat aatcctaat 
      cctagcctt gaattattt ctattacaa atggaaatt 
      tacttactg gcaatgcaa aaaaatccc aactttggc 
      cccaaaaat ccccttcgg cgacctccc caacccttt 
      gaaacaagc ggaagccac gggtgttca catcaaacg 
      tctctatga ccaaatcaa aggcaaagg ctggaaaca 
      cgccggttt gtatttttt ggcacgtcc tgtatacat 
      ggtggtcga cttagagta cgtgaaaaa tatcctaac 
      taaagattt ccaccattt cgtcgatcg gggatttta 
      tttcaacga aaaggacga tccgataag tgcccacgt 
      tttcgcagt cggcggcca aaaatggcg aaacttgag 
      aactaaact aaccccaac tttcacctc cgggaaaat 
      gaaaatgat gttccagac gttactaga atgcgaagc 
      taatttgct aaaacgctt cgaaaagga acacaaaag 
      tgcgatcga tattaaaga tattttggg ctgttttac 
      aactgatat aatcggttc ttgtggttt tggcctcga 
      ttttaacac ttttgagga cgaaaattc gcccttccg 
      taaatacgg gaaaaaagt tttcgcctc aaatctcac 
      aagaaggct ccaaatttt tttcttcac tcatttccc 
      gtcaatggc acgggccct aaaagtgcg ccacttttc 
      caaagacgt ggccgattt tttcacaaa agtggtgaa 
      agacacggt cgagttccg ggagagaaa taattacaa 
      gcgcaagga ttttatgca acttttgat tgatttgaa 
      aaattgcaa ctctggact gaccttgaa cgaaattac 
      ggcccaaag tttgatttt ctttttggc gggttttga 
      aacctcgtc cactaccat gacttttgc actttacga 
      acttgccaa aaacgagaa aatacagga gcgggtgag 
      agacgagat tttttcaat tctgggtga agaaaaaat 
      cacgtatga agcgctcca aaaaatgaa atacttggc 
      acttgttat aaatgaaac tttaaggaa gtacccgcc 
      actgtcttt cctgacgcg ggaatgtgt ccaggatta 
      taaaatccc ggaccagga tgtgatcat agaaaaggg 
      aacaaaagt cgtaatttc aattttggg gatgcacag 
      ggaccagga attttatcc tgatcctga gacattcga 
      tcctgggcg tttttcgga aggtaatgt tgtctcaag 
      acaccagta tgcctatat tccctttgg ggaaggtcc 
      ccgaatttg tatgggttt aaggttcgg aatgatgca 
      aactaaagt cgggttggt ttcgttgtt gcgaaaatt 
      caagtttac agtgaatgg gaggacgaa agaaccaat 
      caagtttga tgtgagggg ttttatttt ggctgcgga 
      gggcgatat ttggctcaa tgcccaaaa acttcactc 
      aaaaatggt tctctccat ggacagatg ggtgggcaa 
      agtggccat cgtcacggg cgccagctc tggaatcgg 
      cgccgccat cgccgacgc tttagtctc caaaggcct 
      cattgttgc tggccttgc ccgccgcac agacctgat 
      cgaaaatcg ggccaaaca gctcaaaaa cggcaaact 
      gtacgctgt taaagccga catgaccgt ggaaaaaga 
      cattctgga cgctttcaa atgggtttc ggaaaattt 
      gggcccggt ccatgtttt aataaacaa tgccgggtt 
      tgccaaaga ggggcttct gaccgaggg cgagacgga 
      aatctggcg gaaaatcct cgaaattaa cgtcctggg 
      tctgtgtat tgccacaag ggaggctgt gaaaatcat 
      gaaaactaa tgggataaa cgggcatgt tatccacat 
      taatagcat tctggggca ttcgattaa tatgttgaa 
      agttaacgt gtatccagc gacgaaatt tggcgttac 
      ggcccttac tgagacctt gaggcagga gttgataag 
      tcttgagag caaaattaa agtcacaag tattagtcc 
      gggtttggt tgccactga aatgacttc tctgaggaa 
      agatttgag tgaggaaag gaaaaaatt gtttgggag 
      ccgaccgtt gctgaaccc tgaagatat cgccgatgg 
      ggtggttta tgccctttc gacccctga gcatgttca 
      agtgcacga actgaccat taaaccact aggggaacc 
      caccgccta atccatgga ccttgacat taaaaaaaa 
      aacattgta tttaactca cttaaccac ttatcttgt 
      taccaaata aataattaa ttcgact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY