Status
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Gene information
General information
TC015192 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1677390..1688778
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015192
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08503

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015192 RA
      MAANMSYCP PGVHIWDVW VNHGTSQCF VETLSSSVI 
      AGFILLAGT IQLCMYRKY GTEISPNHL VQSRLYHFQ 
      IFVTLLVPV LEVVRFVLR GTVFDDKHI YGFMITSLV 
      LNLLAYPFS LLVVRVERH YLLPSVPTK GHGIVLLLF 
      WTLVFISEN LAFFNLDKK EWWFQLKTT EDQIEMGLF 
      VVRYLSCLL IFLLGLKAP GITQNINYF TLNDSTRNL 
      PPQQDRTEN QSTWRNLWK KVRILAPFI WPKKDVLLQ 
      FRVIFCFLL LAGGRVINL YVPIYNKYI VDSMTPDEK 
      LEKLEFRWD WILVYVAFK FLQGGGTGG MGLLNNLRS 
      FLWIRIQQY TTREVEVEL FRHLHSLSL RWHLGRKTG 
      EVLRVMDRG TDSINNLLN YIIFSIFPT IVDIIVAVV 
      FFVVTFNAW FGLIVFITM VLYIALTIS ITEWRTKFQ 
      RRMNLADNE TRARSVDSL LNFETVKYY GAEQYEVEA 
      FREAILRYQ VEEWKSNIT LNILNTVQN IIICTGLLA 
      GSLLCAWMV VEKGELRVG DYVLFATYI VQLYVPLNW 
      FGTYYRAIQ KNFVDMENM FDLLKEDQE VIDAPNAGP 
      IAVKRGAVE FNNVSFGYL PERLVLKNV TFSVPQGKT 
      VALVGPSGS GKSTIIRLL FRFYDVETG SVIIDGQNI 
      KTVTQESLR RAIGVVPQD TVLFNNTIR YNIKYGRLT 
      ATDADVIEA ARGADIHDR ILTFPDAYD TQVGERGLR 
      LSGGEKQRV AIARTLLKA PMIILLDEA TSALDTQTE 
      RNIQESLDR MCANRTTII VAHRLSTVI HADEILVLR 
      DGEIIERGK HEHLIGQEG VYASMWKQQ LESKENSAE 
      TSLERS
    CDS sequence
    • >TC015192 RA
      atggctgca aatatgagc tactgcccc ccgggggtg 
      cacatttgg gacgtgtgg gtgaaccac gggacgtcc 
      caatgcttc gtcgagact ttgtcatcg tctgtgatc 
      gctgggttt attttactg gctggtacc atccagttg 
      tgcatgtac aggaaatat ggtactgaa atatcgcca 
      aaccacttg gttcagtcc cgtttgtac catttccaa 
      atttttgtg acgctgctg gtgcccgtg ttggaagtg 
      gtgaggttc gtgctgcga gggacagtg tttgatgat 
      aaacacatt tatgggttt atgataacg tccctcgtg 
      ctcaacctc ctcgcctac cccttttcc cttctggtg 
      gtccgagtc gaaaggcac taccttttg ccctcggtg 
      cccaccaaa gggcacggt atcgttctt ttactcttc 
      tggactcta gttttcatc tccgaaaac ctcgctttc 
      ttcaatttg gacaagaaa gaatggtgg tttcagctc 
      aaaacgaca gaggaccaa atcgagatg ggccttttc 
      gtcgtacgt tacttatcc tgtctttta atcttttta 
      ctaggctta aaagcgccc ggaatcacc caaaacatc 
      aactatttc acactaaac gactcgact cggaatttg 
      ccgccacag caggaccgt accgagaac caatcaaca 
      tggcgcaat ctttggaag aaagtccga attttggcg 
      ccctttatt tggcccaaa aaggacgtt ttgttgcaa 
      tttcgcgtc attttctgc ttcctgcta ttggccggg 
      gggcgggtc atcaacttg tacgtcccc atttacaac 
      aaatatatc gtggatagt atgacacct gatgagaaa 
      ttagagaag ttggagttt cgctgggac tggattttg 
      gtttacgtt gcgtttaag ttcctccaa ggtgggggg 
      acggggggt atggggttg ttgaataat ttgcgttcg 
      tttctgtgg atcaggata cagcagtat acgacgagg 
      gaggtggag gtggagttg ttcagacat ttgcacagt 
      ttgtcgttg cggtggcat ttggggcgg aagacgggg 
      gaggtgttg agagtgatg gataggggg acggacagt 
      attaataat ttgttgaac tatattatt ttttcgatt 
      tttccgacg attgttgat attattgtg gctgtggtg 
      ttttttgtg gtgactttt aatgcgtgg ttcggattg 
      attgtcttc atcacgatg gtgttatac atagctctc 
      acaatcagc atcaccgaa tggcgtacg aaattccaa 
      cgtcgcatg aaccttgcc gacaacgag acacgtgct 
      cgtagcgtc gactcttta ctaaatttc gaaacggtc 
      aaatattac ggtgccgaa cagtacgaa gttgaagca 
      tttcgtgaa gccatcctg cgctaccaa gtcgaagaa 
      tggaagtcc aacatcact ctcaacatt ttaaacaca 
      gtccagaac attattatt tgtaccgga cttttggcc 
      ggaagtctc ttatgtgcc tggatggtt gtggaaaag 
      ggggagctt agagttggc gattatgtt cttttcgcc 
      acttatatt gtccaactc tatgtccca ttgaactgg 
      tttgggacg tattacagg gcgattcag aaaaatttt 
      gtcgatatg gaaaatatg ttcgatttg ttgaaggag 
      gaccaggag gtgatcgat gcccccaat gcggggccc 
      attgccgtc aaaaggggg gctgtggag ttcaataat 
      gtctcgttt gggtactta cccgaacgg ttggtgctg 
      aaaaatgtc actttttcg gtgcctcaa ggcaaaacc 
      gtggctctg gtcggccct tcgggcagc ggtaagagc 
      acaataatt cgtttattg ttccgtttc tacgacgtc 
      gagaccggt tcggttata atcgacggt cagaacatc 
      aaaacagtg actcaggaa tcgctcaga agggcgatc 
      ggagtcgtg cctcaggac actgtcctg ttcaataac 
      acgatccga tataatatt aaatatgga cgtttgaca 
      gctactgac gctgatgtg attgaagca gcacgtggt 
      gctgatatt catgatcgt attttgacg tttccggac 
      gcttatgac acccaagtt ggtgaaagg gggctgcgg 
      ttaagcggc ggcgagaaa caacgtgtt gctatagca 
      cgtacgttg ctcaaagcc cccatgatt atactgttg 
      gatgaggcg acaagtgcg cttgatacg caaactgag 
      agaaacatc caagagtcc ttggatagg atgtgtgcc 
      aataggacg acgattatt gtggcgcat cgattgtca 
      actgttata catgccgat gagatactt gtcttacgg 
      gacggggaa attatagaa aggggaaaa cacgagcac 
      ttgattggc caggaaggc gtgtacgcc tccatgtgg 
      aagcaacaa ttggagagt aaagaaaat tcagccgag 
      actagcctt gagaggagt taa
    mRNA sequence
    • >TC015192 RA
      tatcaccaa agtcgtaat atagtattt cgttacgtt 
      ccattaacg cagcatagt ttttgcgtg ttattatca 
      ttaaaaagc ctcgctcgt aacctaact ttcttatgt 
      cattcactg gaaatgtca cattgtgat gactaaaca 
      ataataatc caatttgca agcgcgtta tcgccttcg 
      gcttacgcc acagcccct tttttacat ttggcggca 
      atgtattag tgggtgaga tggctgcaa atatgagct 
      actgccccc cgggggtgc acatttggg acgtgtggg 
      tgaaccacg ggacgtccc aatgcttcg tcgagactt 
      tgtcatcgt ctgtgatcg ctgggttta ttttactgg 
      ctggtacca tccagttgt gcatgtaca ggaaatatg 
      gtactgaaa tatcgccaa accacttgg ttcagtccc 
      gtttgtacc atttccaaa tttttgtga cgctgctgg 
      tgcccgtgt tggaagtgg tgaggttcg tgctgcgag 
      ggacagtgt ttgatgata aacacattt atgggttta 
      tgataacgt ccctcgtgc tcaacctcc tcgcctacc 
      ccttttccc ttctggtgg tccgagtcg aaaggcact 
      accttttgc cctcggtgc ccaccaaag ggcacggta 
      tcgttcttt tactcttct ggactctag ttttcatct 
      ccgaaaacc tcgctttct tcaatttgg acaagaaag 
      aatggtggt ttcagctca aaacgacag aggaccaaa 
      tcgagatgg gccttttcg tcgtacgtt acttatcct 
      gtcttttaa tctttttac taggcttaa aagcgcccg 
      gaatcaccc aaaacatca actatttca cactaaacg 
      actcgactc ggaatttgc cgccacagc aggaccgta 
      ccgagaacc aatcaacat ggcgcaatc tttggaaga 
      aagtccgaa ttttggcgc cctttattt ggcccaaaa 
      aggacgttt tgttgcaat ttcgcgtca ttttctgct 
      tcctgctat tggccgggg ggcgggtca tcaacttgt 
      acgtcccca tttacaaca aatatatcg tggatagta 
      tgacacctg atgagaaat tagagaagt tggagtttc 
      gctgggact ggattttgg tttacgttg cgtttaagt 
      tcctccaag gtgggggga cggggggta tggggttgt 
      tgaataatt tgcgttcgt ttctgtgga tcaggatac 
      agcagtata cgacgaggg aggtggagg tggagttgt 
      tcagacatt tgcacagtt tgtcgttgc ggtggcatt 
      tggggcgga agacggggg aggtgttga gagtgatgg 
      ataggggga cggacagta ttaataatt tgttgaact 
      atattattt tttcgattt ttccgacga ttgttgata 
      ttattgtgg ctgtggtgt tttttgtgg tgactttta 
      atgcgtggt tcggattga ttgtcttca tcacgatgg 
      tgttataca tagctctca caatcagca tcaccgaat 
      ggcgtacga aattccaac gtcgcatga accttgccg 
      acaacgaga cacgtgctc gtagcgtcg actctttac 
      taaatttcg aaacggtca aatattacg gtgccgaac 
      agtacgaag ttgaagcat ttcgtgaag ccatcctgc 
      gctaccaag tcgaagaat ggaagtcca acatcactc 
      tcaacattt taaacacag tccagaaca ttattattt 
      gtaccggac ttttggccg gaagtctct tatgtgcct 
      ggatggttg tggaaaagg gggagctta gagttggcg 
      attatgttc ttttcgcca cttatattg tccaactct 
      atgtcccat tgaactggt ttgggacgt attacaggg 
      cgattcaga aaaattttg tcgatatgg aaaatatgt 
      tcgatttgt tgaaggagg accaggagg tgatcgatg 
      cccccaatg cggggccca ttgccgtca aaagggggg 
      ctgtggagt tcaataatg tctcgtttg ggtacttac 
      ccgaacggt tggtgctga aaaatgtca ctttttcgg 
      tgcctcaag gcaaaaccg tggctctgg tcggccctt 
      cgggcagcg gtaagagca caataattc gtttattgt 
      tccgtttct acgacgtcg agaccggtt cggttataa 
      tcgacggtc agaacatca aaacagtga ctcaggaat 
      cgctcagaa gggcgatcg gagtcgtgc ctcaggaca 
      ctgtcctgt tcaataaca cgatccgat ataatatta 
      aatatggac gtttgacag ctactgacg ctgatgtga 
      ttgaagcag cacgtggtg ctgatattc atgatcgta 
      ttttgacgt ttccggacg cttatgaca cccaagttg 
      gtgaaaggg ggctgcggt taagcggcg gcgagaaac 
      aacgtgttg ctatagcac gtacgttgc tcaaagccc 
      ccatgatta tactgttgg atgaggcga caagtgcgc 
      ttgatacgc aaactgaga gaaacatcc aagagtcct 
      tggatagga tgtgtgcca ataggacga cgattattg 
      tggcgcatc gattgtcaa ctgttatac atgccgatg 
      agatacttg tcttacggg acggggaaa ttatagaaa 
      ggggaaaac acgagcact tgattggcc aggaaggcg 
      tgtacgcct ccatgtgga agcaacaat tggagagta 
      aagaaaatt cagccgaga ctagccttg agaggagtt 
      aatccccac cccaccccc cactattta caattagtt 
      gccgaggtt tggggctta ttaagtgaa aaaaaacgc 
      ttcgtaatt attgattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY