Status
Please wait ...
Gene information
General information
TC015503 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1592079..1609656
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015503
    iBeetle screen iB_08501

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015503 RA
      MTDHRLLPP TDIPLIITS TSSSPESSR KIGDRRKAS 
      SVGSEKNGK NVNLQAGGS FKYEIGRNR SVSNLINLP 
      AKWSSVRIK GTGFKESRK SVVKLESCN GLPLKKEFQ 
      NLLSPQNQV KKNIGDVLS LGADVLPEY KLQNPRIHN 
      WTILHYSPF KAVWDWIIL ILVMYTAIF TPYVAAFLL 
      NEPDNNRKN KKYGEDPIV IIDLIVDVT FIIDILINF 
      RTTYVSGND EIVSDPVRI AIHYLKGWF FIDLVAAVP 
      FDLLFFGSD TDETTTLIG LLKTARLLR LVRVARKID 
      RYSEYGAAV LLLLMATFA LIAHWLACI WYAIGNAEN 
      HSKIGWLEI LANDTHQFY GPNNTGGPS IKSRYVTAL 
      YFTFTSLTS VGFGNVAPN TDAEKIFTI CVMLAGSLM 
      YASIFGNVS AIIQRLYSG TARYHTQML RVREFIRFH 
      QIPNPLRQR LEEYFQHAW AFTNGIDMN SVLKGFPEC 
      LQADICLHL NRNLLQNCS VFDGASPGC LRAFTLKFK 
      TTHAPPGDT LVHRGDVLS SLYFISRGS IEILRDDIV 
      MAILGKYDI FGENPCLHP TLGKSSCNV RALTYCDLH 
      KIHRDDLLD VLELYPEFY NQFMNNLEI TFNLRDEEQ 
      AGVIPRRSF YPKSHTKES RYFRGVNSN GVSSRGNSM 
      TGRQDSQYS DESESGHGI LEFSTEKAG QDVTPINLD 
      FGEEKKRSS TLNSITGML SQLKRSIPD LRLHKTHQP 
      LIAQSTPPS SHRIRTIRR QSSVGTYQE SAHQQQQPQ 
      PQVPISSST SYYTNSPSP PAPHVVEAT QHSIETHSD 
      LVVSKGLEN IHSKLEQLT RRVQQLEVS LAGDVKTIL 
      GLLQGGDRG HEMPEYENL DPSRIRYTF QRSVSEPKP 
      ISKSQQHSQ VLHRFASFN KAFDFDKFQ DSSWADTLV 
      GKEKESKED KSAPIAKLE SLDELDLES PQGSPKKPK 
      K
    CDS sequence
    • >TC015503 RA
      atgactgac caccgactt ctcccccca acggacatc 
      ccgttgata ataacgtcg acaagttcg agtccggaa 
      tcatcccgg aaaatcggc gatcgccgg aaagccagt 
      tcagtcggt agtgaaaaa aacggcaaa aatgtgaat 
      ttacaagcc ggcggctcg ttcaaatac gagatcgga 
      agaaatcgc tcggtttcg aatctgatc aatttgccg 
      gcgaaatgg tcgagcgtg aggattaaa ggcactgga 
      ttcaaggag tcaaggaaa agcgtcgtt aagctggaa 
      tcgtgtaat ggattaccg ctgaagaag gagttccag 
      aacttgctc agtccccag aatcaggtg aagaagaat 
      ataggagat gtgctgtcg ttgggggcc gatgtactt 
      ccagagtac aaattgcaa aatccaagg atacataat 
      tggaccatt ttgcattac tcgcccttc aaggccgtc 
      tgggactgg atcatactg attttggtg atgtacact 
      gcgatattt acgccttat gttgccgct tttttactc 
      aacgagcca gataataac aggaaaaat aaaaagtac 
      ggggaggat cctattgtt attatagat ttgatagtg 
      gatgtaacg tttatcatc gacattctt atcaacttt 
      cggacgact tacgtcagt ggtaacgac gaaatcgtc 
      tcagacccg gtccgaata gccatccac tacctaaaa 
      gggtggttt tttatcgac ttggtggct gcggtgcct 
      ttcgatttg ttgtttttc ggaagcgac acggacgaa 
      acgacaact ctgattgga ttactaaag actgctcgc 
      cttttgcgt ttggtccga gttgcgcga aaaatcgac 
      cgatattcc gaatatgga gccgccgtt ttgctgctt 
      ctgatggcc acgtttgcg ctcatcgcc cattggctg 
      gcctgcata tggtacgcc atcgggaac gcagaaaac 
      cactcgaaa atcggatgg ttggagatt ctcgccaat 
      gacacgcat cagttctac gggccgaat aatacaggg 
      gggcccagc attaaaagt cggtatgtg acggccctg 
      tatttcacc ttcacgtcg ctgaccagt gtgggcttt 
      ggcaacgtt gccccgaat acggacgcc gagaagatt 
      tttaccatc tgtgtgatg ctggctggg tccctgatg 
      tacgccagc atcttcggc aacgtttcg gccatcatc 
      cagcgccta tactcaggc acagctcgt taccacact 
      cagatgctg cgcgtccgt gaatttatt cgcttccat 
      cagattcca aatcctctg agacagcgt ctggaggag 
      tacttccag cacgcttgg gccttcacc aacggcatc 
      gacatgaac tcggtgctc aagggcttc cccgagtgt 
      ctccaagcc gacatttgt ctccatctc aaccgaaat 
      ctgctacag aattgcagc gtttttgac ggagcgagt 
      cccgggtgt cttcgtgct ttcaccttg aagttcaag 
      accacgcac gcaccgccg ggggatacc ctggtgcac 
      aggggggat gtactcagt tcgttgtat ttcatttca 
      agggggtcg attgagatt ctgagagat gatattgtg 
      atggccatt ttaggcaaa tatgatatt ttcggggaa 
      aacccgtgc ttacatccc acattggga aagtcaagc 
      tgcaacgtt cgggctttg acgtactgt gatttacat 
      aagatacat agagatgat ctcctagac gtgctagag 
      ctctatccc gagttttac aaccagttt atgaataat 
      ttagaaatt acgtttaat ttaagggat gaggagcaa 
      gccggtgtt atcccccgg cgctctttc taccccaaa 
      agccacaca aaagagagt cgctatttt cgaggagtc 
      aactcgaat ggagtttcc agtcggggg aactcaatg 
      accggacgc caggactcc caatacagc gacgagtcc 
      gagtcagga catggaatt ttggagttt tcgacggaa 
      aaagccggt caggacgtg acaccaatc aatttggac 
      tttggcgaa gagaagaag cgatcgtcc actttgaac 
      tcaatcaca ggcatgcta tcacagctc aaaagaagt 
      atacccgat ctaaggtta cacaaaacg catcagccg 
      ctcatagcc caatcaacg cccccttcc agccaccgc 
      atccgcacc atccgtcgc caatcctcc gtgggcacg 
      taccaggag tccgcccac cagcagcag cagccccag 
      ccccaggtg cccatctcc agcagcacc agctactac 
      accaacagc ccctccccg ccggccccc cacgtcgtc 
      gaagccacc caacattcc atagagaca cattctgac 
      ttagtagtg agtaaaggt ttggagaac atccattcg 
      aaattggag cagttgaca agacgggtg caacagctg 
      gaggtgagt cttgccggg gacgtcaag accatcctg 
      gggctgctg caagggggc gacaggggg cacgaaatg 
      cccgaatat gaaaatcta gatcctagt agaattagg 
      tatacgttt cagcggtcg gtgagtgaa ccgaaaccg 
      atctcgaaa agtcaacag cattcccaa gtacttcat 
      aggtttgca tcgtttaat aaagctttt gatttcgat 
      aagttccaa gacagctcg tgggctgat actctcgta 
      ggcaaggaa aaagaaagt aaggaagac aagtcggcc 
      cccattgcc aaattagag tcactcgat gagctggat 
      ttggagtcg ccacaaggc agtccgaag aaacccaaa 
      aaatag
    mRNA sequence
    • >TC015503 RA
      ttagtatca cacaaaacc gttccaaga caacgtcaa 
      taactcagt tcgttgcca atcattaca caaaactat 
      gaatgaaaa cgcgctcaa agtgtccca agtgctcag 
      tgcgacccc ggtggcagg acgtctgga aaaatatgc 
      aaaataaaa cgtgattat tgaaaatgt aacaatgac 
      tgaccaccg acttctccc cccaacgga catcccgtt 
      gataataac gtcgacaag ttcgagtcc ggaatcatc 
      ccggaaaat cggcgatcg ccggaaagc cagttcagt 
      cggtagtga aaaaaacgg caaaaatgt gaatttaca 
      agccggcgg ctcgttcaa atacgagat cggaagaaa 
      tcgctcggt ttcgaatct gatcaattt gccggcgaa 
      atggtcgag cgtgaggat taaaggcac tggattcaa 
      ggagtcaag gaaaagcgt cgttaagct ggaatcgtg 
      taatggatt accgctgaa gaaggagtt ccagaactt 
      gctcagtcc ccagaatca ggtgaagaa gaatatagg 
      agatgtgct gtcgttggg ggccgatgt acttccaga 
      gtacaaatt gcaaaatcc aaggataca taattggac 
      cattttgca ttactcgcc cttcaaggc cgtctggga 
      ctggatcat actgatttt ggtgatgta cactgcgat 
      atttacgcc ttatgttgc cgctttttt actcaacga 
      gccagataa taacaggaa aaataaaaa gtacgggga 
      ggatcctat tgttattat agatttgat agtggatgt 
      aacgtttat catcgacat tcttatcaa ctttcggac 
      gacttacgt cagtggtaa cgacgaaat cgtctcaga 
      cccggtccg aatagccat ccactacct aaaagggtg 
      gttttttat cgacttggt ggctgcggt gcctttcga 
      tttgttgtt tttcggaag cgacacgga cgaaacgac 
      aactctgat tggattact aaagactgc tcgcctttt 
      gcgtttggt ccgagttgc gcgaaaaat cgaccgata 
      ttccgaata tggagccgc cgttttgct gcttctgat 
      ggccacgtt tgcgctcat cgcccattg gctggcctg 
      catatggta cgccatcgg gaacgcaga aaaccactc 
      gaaaatcgg atggttgga gattctcgc caatgacac 
      gcatcagtt ctacgggcc gaataatac aggggggcc 
      cagcattaa aagtcggta tgtgacggc cctgtattt 
      caccttcac gtcgctgac cagtgtggg ctttggcaa 
      cgttgcccc gaatacgga cgccgagaa gatttttac 
      catctgtgt gatgctggc tgggtccct gatgtacgc 
      cagcatctt cggcaacgt ttcggccat catccagcg 
      cctatactc aggcacagc tcgttacca cactcagat 
      gctgcgcgt ccgtgaatt tattcgctt ccatcagat 
      tccaaatcc tctgagaca gcgtctgga ggagtactt 
      ccagcacgc ttgggcctt caccaacgg catcgacat 
      gaactcggt gctcaaggg cttccccga gtgtctcca 
      agccgacat ttgtctcca tctcaaccg aaatctgct 
      acagaattg cagcgtttt tgacggagc gagtcccgg 
      gtgtcttcg tgctttcac cttgaagtt caagaccac 
      gcacgcacc gccggggga taccctggt gcacagggg 
      ggatgtact cagttcgtt gtatttcat ttcaagggg 
      gtcgattga gattctgag agatgatat tgtgatggc 
      cattttagg caaatatga tattttcgg ggaaaaccc 
      gtgcttaca tcccacatt gggaaagtc aagctgcaa 
      cgttcgggc tttgacgta ctgtgattt acataagat 
      acatagaga tgatctcct agacgtgct agagctcta 
      tcccgagtt ttacaacca gtttatgaa taatttaga 
      aattacgtt taatttaag ggatgagga gcaagccgg 
      tgttatccc ccggcgctc tttctaccc caaaagcca 
      cacaaaaga gagtcgcta ttttcgagg agtcaactc 
      gaatggagt ttccagtcg ggggaactc aatgaccgg 
      acgccagga ctcccaata cagcgacga gtccgagtc 
      aggacatgg aattttgga gttttcgac ggaaaaagc 
      cggtcagga cgtgacacc aatcaattt ggactttgg 
      cgaagagaa gaagcgatc gtccacttt gaactcaat 
      cacaggcat gctatcaca gctcaaaag aagtatacc 
      cgatctaag gttacacaa aacgcatca gccgctcat 
      agcccaatc aacgccccc ttccagcca ccgcatccg 
      caccatccg tcgccaatc ctccgtggg cacgtacca 
      ggagtccgc ccaccagca gcagcagcc ccagcccca 
      ggtgcccat ctccagcag caccagcta ctacaccaa 
      cagcccctc cccgccggc cccccacgt cgtcgaagc 
      cacccaaca ttccataga gacacattc tgacttagt 
      agtgagtaa aggtttgga gaacatcca ttcgaaatt 
      ggagcagtt gacaagacg ggtgcaaca gctggaggt 
      gagtcttgc cggggacgt caagaccat cctggggct 
      gctgcaagg gggcgacag ggggcacga aatgcccga 
      atatgaaaa tctagatcc tagtagaat taggtatac 
      gtttcagcg gtcggtgag tgaaccgaa accgatctc 
      gaaaagtca acagcattc ccaagtact tcataggtt 
      tgcatcgtt taataaagc ttttgattt cgataagtt 
      ccaagacag ctcgtgggc tgatactct cgtaggcaa 
      ggaaaaaga aagtaagga agacaagtc ggcccccat 
      tgccaaatt agagtcact cgatgagct ggatttgga 
      gtcgccaca aggcagtcc gaagaaacc caaaaaata 
      gcttttcgt ttttaaacc cgagcgagt ttttagact 
      gtcgtaaca gcagggatg gtcatttgg gcgtaaaaa 
      cgtgtcgaa gttcgattt tgaggtaat agttgttac 
      ctaccgact gcgccaaat tctgttaca gcacaataa 
      cacgaaaaa tttcacact ttttacgtc aactgatcc 
      ctggaaacc agtgagatt atgtaataa taagccccg 
      aatttggaa attttaggg atatttcag cttacaata 
      gagaaattt ccattttcg cctccggtt cgactggcg 
      ctgcgtccg gcaaaaaaa gtcacttga ttaattgat 
      taaaaaatt caacatatc gttttggtg attagtaga 
      agccttggc aatggcctt atttccgta gcaattcat 
      ttagcaata ataagttgc gtgttggtg acgcggcgc 
      cagtctgcg agttaaata tccgtctca aaatctacg 
      ataaggttg aatcttttg tataataaa aggtggtaa 
      aatgaaaga caattttca aatatttcc ctaattgtt 
      tgttgagat tatttgtta cgcttgttt ttcgcatca 
      aatcgcttt tatgaaatt ttgtaagca caaattatt 
      tgtttatac acaatttta tcatatttt taataatat 
      tatgtaatt aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY