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Gene information
General information
TC013585 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3424920..3427552
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013585
    iBeetle screen iB_08500

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013585 RA
      MVVMVVTVK EICDNVTLA REMAMLGNY ESAEVYYEG 
      SIQMISRLI LMIPEPLRK NKWQQVQKK VASEYNEIK 
      VLKSMLQTL RLETNVEVP LGARRLRDE PVHDSLDLQ 
      EDTFGAPHD PDVWPPPTP VDHGFMQRN SFSKPRLSN 
      VKKNEPKKG ARASSSNTA RRSDVRPIK TGKKEDRPS 
      SSKSERNPE GKPDKDKDV DNNEKNDKA ESEEEKKFE 
      CHGMERELA DVLERDIVQ KNPNIRWDD IADLHEAKR 
      LLEEAVVLP MWMPEFFKG IRRPWKGVL MVGPPGTGK 
      TMLAKAVAT ECSTTFFNV SSSTLTSKY RGESEKMVR 
      LLFEMARFY APSTIFIDE IDSLCSRRG SESEHEASR 
      RVKSELLVQ MDGITANND EPGKVVMVL AATNFPWDI 
      DEALRRRLE KRIYIPLPT QEGREALLK INLREVKLD 
      PDVNLSDIA EKLDGFSGA DITNVCRDA SMMSMRRKI 
      YGLKPDQIK QLPKEELDL PVTNRDFEE ALLKNNKSV 
      SKDDLDKYE KWMNEFGSS
    CDS sequence
    • >TC013585 RA
      atggtagtg atggttgtt actgttaag gaaatatgt 
      gataatgtt actctggca cgagaaatg gcaatgctt 
      ggcaattac gaaagtgca gaggtttat tatgaaggg 
      tccatccag atgattagt cgcttaatt ctcatgata 
      cccgaaccc ttacgcaaa aataagtgg caacaagtt 
      caaaaaaaa gttgcttct gaatacaat gagataaaa 
      gtgttgaaa tcgatgttg caaacgctg cgtttggag 
      actaacgtt gaagtccct cttggtgca agaaggttg 
      agagatgag ccggttcat gatagcctt gatctccag 
      gaagatact ttcggggct cctcatgat cccgacgtt 
      tggccgcca ccaactcct gtagatcac ggttttatg 
      cagaggaac agtttctca aaacctcga ttgtctaat 
      gtgaaaaag aatgaacct aagaaggga gccagagcc 
      agctcttca aacactgcg cgaaggtct gacgtgaga 
      ccgatcaaa actggaaag aaagaggac agaccttcg 
      tcttctaaa tcagaaagg aatccggaa ggaaaaccg 
      gacaaggat aaagatgtg gacaataat gaaaagaat 
      gataaggcc gagtctgag gaagagaag aaattcgag 
      tgtcacgga atggaacga gaactagcc gacgtcttg 
      gagcgagat atcgtccaa aaaaacccc aacattcgt 
      tgggatgat attgccgac ttgcatgaa gctaaaaga 
      ctactggaa gaggctgta gttcttcca atgtggatg 
      cctgagttt tttaaaggt ataaggaga ccgtggaaa 
      ggtgtttta atggtgggc ccgcccgga acgggcaaa 
      acaatgttg gctaaagcg gtggctact gagtgcagc 
      acgactttc tttaatgta tcttcatcc actctcaca 
      tcaaagtac agaggtgaa tcagagaaa atggtcaga 
      ctgttgttt gaaatggct aggttttat gccccaagt 
      actatattt atagacgaa atagactct ctctgctct 
      cgcagaggt tcagaatct gagcatgag gcgtccaga 
      cgcgtcaaa tccgagctt ttggtgcag atggatggc 
      attactgcc aataatgat gaaccggga aaagttgtt 
      atggtcctt gcggctact aactttccg tgggacata 
      gacgaggct cttcggaga cgtttagaa aaacgcata 
      tatattcca ttgccgact caagaaggt cgtgaagca 
      ttacttaag ataaacttg cgcgaagtt aagttggat 
      cctgatgtg aatttgagc gacattgcg gaaaagttg 
      gacggtttt tcaggcgct gatataaca aatgtgtgt 
      agggatgca tctatgatg tccatgagg cgcaaaatt 
      tatggcttg aaacctgac cagatcaaa cagttgccg 
      aaggaagaa cttgatttg cctgtcact aatagagat 
      ttcgaggag gccttactt aaaaataat aaaagcgtt 
      tcaaaggac gatctggac aagtatgag aagtggatg 
      aatgagttt ggatcatca tga
    mRNA sequence
    • >TC013585 RA
      tatagtttt aaatgatta actcaaaaa agaaataat 
      aaaaactac cttatttca ttattacta attatgttt 
      attttctat tttcctgtt tgaattttc tgttagcaa 
      tgtcccaaa ataaaatgg tggaactcc agttgtcaa 
      agtcaaacg tcaaacgtc tatttcaag tcaaatgat 
      attttaatc aaaaaatca aaaacaggg cttttgtgg 
      gaacataat ataacattt ataaaaaaa aatcgactt 
      gatacaggt atggtagtg atggttgtt actgttaag 
      gaaatatgt gataatgtt actctggca cgagaaatg 
      gcaatgctt ggcaattac gaaagtgca gaggtttat 
      tatgaaggg tccatccag atgattagt cgcttaatt 
      ctcatgata cccgaaccc ttacgcaaa aataagtgg 
      caacaagtt caaaaaaaa gttgcttct gaatacaat 
      gagataaaa gtgttgaaa tcgatgttg caaacgctg 
      cgtttggag actaacgtt gaagtccct cttggtgca 
      agaaggttg agagatgag ccggttcat gatagcctt 
      gatctccag gaagatact ttcggggct cctcatgat 
      cccgacgtt tggccgcca ccaactcct gtagatcac 
      ggttttatg cagaggaac agtttctca aaacctcga 
      ttgtctaat gtgaaaaag aatgaacct aagaaggga 
      gccagagcc agctcttca aacactgcg cgaaggtct 
      gacgtgaga ccgatcaaa actggaaag aaagaggac 
      agaccttcg tcttctaaa tcagaaagg aatccggaa 
      ggaaaaccg gacaaggat aaagatgtg gacaataat 
      gaaaagaat gataaggcc gagtctgag gaagagaag 
      aaattcgag tgtcacgga atggaacga gaactagcc 
      gacgtcttg gagcgagat atcgtccaa aaaaacccc 
      aacattcgt tgggatgat attgccgac ttgcatgaa 
      gctaaaaga ctactggaa gaggctgta gttcttcca 
      atgtggatg cctgagttt tttaaaggt ataaggaga 
      ccgtggaaa ggtgtttta atggtgggc ccgcccgga 
      acgggcaaa acaatgttg gctaaagcg gtggctact 
      gagtgcagc acgactttc tttaatgta tcttcatcc 
      actctcaca tcaaagtac agaggtgaa tcagagaaa 
      atggtcaga ctgttgttt gaaatggct aggttttat 
      gccccaagt actatattt atagacgaa atagactct 
      ctctgctct cgcagaggt tcagaatct gagcatgag 
      gcgtccaga cgcgtcaaa tccgagctt ttggtgcag 
      atggatggc attactgcc aataatgat gaaccggga 
      aaagttgtt atggtcctt gcggctact aactttccg 
      tgggacata gacgaggct cttcggaga cgtttagaa 
      aaacgcata tatattcca ttgccgact caagaaggt 
      cgtgaagca ttacttaag ataaacttg cgcgaagtt 
      aagttggat cctgatgtg aatttgagc gacattgcg 
      gaaaagttg gacggtttt tcaggcgct gatataaca 
      aatgtgtgt agggatgca tctatgatg tccatgagg 
      cgcaaaatt tatggcttg aaacctgac cagatcaaa 
      cagttgccg aaggaagaa cttgatttg cctgtcact 
      aatagagat ttcgaggag gccttactt aaaaataat 
      aaaagcgtt tcaaaggac gatctggac aagtatgag 
      aagtggatg aatgagttt ggatcatca tgatagctc 
      attgacaga acacggttt gattcattg tgtgatatt 
      ataatgctt tacagttta tatttacac gaattttat 
      ctttacagt tatttaata ctttttatg cttatctaa 
      attttatta ttggaagta ttaatttca agtaacaaa 
      cagtagatt tatgaatta gctacaaag ttttgcact 
      ttatcgaat tgtaaattg ccattgtat cacggattt 
      tgtatttat gtatatctt agatcatgt ttatctggt 
      taatggtca attgatttg tactgtata tattatgat 
      aatatgata cctgatgtt ctaacaatt aattcaagt 
      aatgccata attttgagt ggttcagtt gttttatta 
      agttcgtaa aaatgtttg gtataagag acagtgtaa 
      tgaatcgtt ttaacaatt ttttgtata aattgacaa 
      aataaataa tacatttaa ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY