| General information | |||
| TC031507
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 3397535..3402462 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031507 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08496 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031507 RA
MSLRKRFRE SPAQETGDN TKKHKRTST HSDKCKFCA QTLNQAVVY TNFPDDYAE ESVALTSER LNVYNGTEN ISFEEGDLP SHKITCFSI YDLNDHLCP LDTGLLQKE VYLHIGGYI KPIFDDDPS PENGIATLD MGPIHEWFI AGFDGGEKV LLGIITNYA QYVLMEPSL EYAPIFKSL QEKTVLFKF VIEFLLSNF SRAPSYEEL LAAVENSDN PLLTEEFLL QNAQFVCDQ IHSFDLDAG DNNDMPLLT MPCVRTLAS LAGVSFRKR QLMRNLLGN QRRTKLQLS KATATPLVR QLFEQLFPN QIQTENCQS PKKTRCGIC DTCRSPDCG QCVYCKDMV KFGGDGNMK QPCKLRRCP NLVVQELDE SDDETVVTP ARGREDGRR PGVSRVLSD GPLQNFRES IDIVLGGVN DDDTGILLN IKTGDFVQL SPKSATKPN TIARVVNIY TATEPMVHV YLFYRGNET ILGEVANPQ ELFASNECE DCPVAAIVG QAKVVYKAT PDNWADLGG IECLPSGDD RFFYAKQYD SETATFTDY VKVRNELDS CGHCRLNEE RKKIETPTF KDNKVQWRN EFYEAGNSV FLDPSVYQF PIPVECTFD DAKEMNESK YPEYYRKRE ANASCKKMP QPFCIGLIE TLCDGPAGV LMAIRVFFR PENTKGGSM LSYQSDINL LFWSTKFIT VPFRKVMGK CYVVYCSDE QKAREWSEG GPYRYYFRQ QYDPDCGEL VPPTYSCTR LALREGAGQ DERPFGRPL KCLDVFAGC GGLSQGFHA AGVANTKWA IENDKPALD TFRHNNRTC HVFRDDCNV LLRNVMSGK GGLPPKSEV EMIVGGPPC QGFSGMNRF NEGEYSLFK NSLVVSLLS LCDYYRPLI FVLENVRNF MLYKGGLIL KLTLQCLVA IGYQVRISI VEAGEFGVP QARRRFILI ASAPGYQLP RIPEPQHVF LQRGSRLDI YVDGVKYTN GNFWTQSAP YRMLHVRDA IADLPAIEH DDNRPQMPY DDDEGTSHF QRKMRGNQE ELLDHICKP IAPIVQTRI KLIPPKGGA DWRNLPNVR VQLPDGTMT EVLQYRYRT KKQKDGEPN RGVCACSVE KACDPNDRQ SNTLIPWCL PHTADRHNN WAGVYGRLD WSGYFATTT TNPEPMGKQ GRVIHPDQN RLISVRECA RSQGFPDKT KFFGSVTSK YRQIGNAVP PPLAKAIGL EIKRALRNR HR
- >TC031507 RA
atgtcctta agaaaaagg tttagagag tctcccgca caggaaacg ggggacaac acgaaaaaa cacaaacga acctctact cattcagac aagtgcaaa ttttgcgca caaactctt aatcaggca gtagtttac acaaatttc cccgatgac tatgccgag gagtctgtt gctttaaca tccgaaaga ctgaatgtg tacaacggt accgaaaac atcagtttt gaggagggc gacttgccc agtcacaaa ataacttgt ttcagtatt tacgacttg aatgaccac ttgtgcccc cttgacacc gggttgttg caaaaagag gtctatttg catatcggg ggttacatt aaaccaatt ttcgacgac gacccgagc cccgaaaat ggcattgca acgcttgac atggggccc attcacgag tggttcatt gccggtttt gacgggggc gagaaagtc ttactgggg attatcact aactacgcc cagtacgtt ttaatggag ccctcgctg gaatacgcc cccattttc aaaagcctc caagagaaa accgttttg tttaaattc gttattgag ttcctacta agcaatttc agccgcgct ccttcctac gaggagcta ctggccgca gtggaaaac agcgataac ccactgttg accgaggaa tttctcctg caaaacgcc cagtttgtg tgcgatcag atccacagc ttcgacttg gacgctggg gacaacaac gacatgccg ttactcaca atgccatgc gttcgaact ttggcgagt ttggctggt gttagcttc cggaagcgc caactcatg cggaatctt ttagggaac caaaggaga actaagctc cagttgagc aaagccaca gccacgccg ctagtccgc caattgttc gaacagttg ttcccgaat caaatccag actgaaaat tgccagtcg cccaaaaag acacggtgt ggcatttgc gacacgtgt cggtcgccc gactgcggc cagtgcgtt tactgcaaa gacatggtc aagttcggc ggtgatggc aacatgaaa cagccctgc aagctgaga cgttgcccc aacttggtc gtgcaagag ttggacgag agtgacgac gaaactgtc gtgacacca gccaggggg cgggaggac ggccgcaga cctggcgtg agccgggtc ctgagcgac ggccccctt cagaatttc cgcgaatcg attgatata gttcttggt ggggttaat gatgacgac acgggcatt ttactcaac atcaagacc ggcgatttt gtccagtta tcgcccaaa agtgcaacc aaaccgaac acaatcgcc cgagttgtt aacatatac acggctacg gaaccaatg gtccatgtg tatttgttc taccgcggc aatgaaacg attttaggg gaagtagcc aatccgcaa gaacttttc gcttcaaat gaatgtgag gattgcccg gttgccgcc atagtagga caggccaag gttgtttat aaagcaacg cccgacaat tgggccgat ttgggcggg attgagtgt ttgccatct ggcgatgat aggtttttt tacgcaaaa cagtacgac agtgagact gccacgttc acggactat gttaaagtc aggaatgag ttggattcg tgcggacat tgcaggttg aacgaggaa cgaaagaaa attgaaact cccactttc aaagataac aaagtccaa tggcgcaac gagttttac gaagcaggg aactcggtt tttttggat ccttcggtt tatcaattt ccgattccc gttgagtgc actttcgat gatgcgaaa gagatgaat gagagcaaa tacccggag tactaccga aaacgcgaa gctaacgct tcttgcaaa aaaatgccc caaccgttc tgcattgga ttaatcgag accttatgc gatgggccc gccggggta ttgatggcg attcgggtg ttttttcgg cctgaaaac acaaagggt ggcagcatg ttatcgtat cagtctgac atcaatctg ctgttttgg tccactaaa tttataaca gttccgttt cgtaaagtg atggggaag tgctatgtt gtttactgt tctgacgaa cagaaggcg cgggaatgg tccgagggg ggcccttat cgctactat ttcagacag caatacgat ccagactgt ggtgaatta gttccaccc acgtatagc tgcacaaga ctggctttg agagaggga gctggccaa gacgaacgt ccttttggc aggccgctg aaatgcctg gacgttttc gcgggctgt gggggactg tcacagggc tttcacgct gcaggcgtc gcaaacacc aaatgggcc attgagaac gacaaaccc gcactggac acgttccgt cataacaat cggacttgc catgttttt cgagacgat tgtaacgtg cttttacgg aatgtcatg tctggaaaa gggggcctg ccccccaag agcgaggtt gaaatgatc gtgggcggg cccccatgc caggggttt tctgggatg aaccgattc aacgaaggc gaatactcg ttgtttaaa aactcgctc gtagtttcg cttctaagt ttgtgcgat tactaccga cccttgata ttcgtattg gagaacgtg cgtaatttc atgctgtac aagggcggg cttattttg aagctgacg ctccaatgt ctcgtggcc atcggctac caggtgcga atttcaatc gtggaagcg ggcgaattc ggcgtgccc caggcccgc aggcgcttt atcctaata gcgtcagcc cccgggtat caattgccc cgcattccg gagcctcaa cacgttttt ctccagaga ggctcgcgt ctagatatt tacgtagac ggtgttaaa tacacgaac ggtaatttc tggacccag tcagcgcct tatcgcatg cttcacgtg cgtgatgcc atcgctgat ctgccagcc attgaacac gatgataac cgcccccaa atgccgtat gacgacgac gaaggaacg tcacatttc caaaggaaa atgcgcggt aatcaggag gagctactg gaccatatt tgcaaaccg atcgccccc attgtgcaa actagaatc aagctaatt ccgccaaaa ggcggtgcc gactggagg aatttgcca aatgtgcgg gtgcaactc ccggatggg accatgact gaggtgctc cagtaccgg tacaggacc aagaagcag aaagacggg gagccgaac cgcggcgtt tgcgcgtgt tcggttgaa aaggcgtgc gatccgaat gatcgccaa tcaaatact ttgattccg tggtgtttg ccacacact gccgatagg cacaataac tgggccggg gtgtacggc cggcttgac tggagcggg tacttcgcc acgaccacc actaacccc gaacccatg gggaaacaa gggcgagtg atacacccg gaccaaaac cgactgata agcgtgcgg gaatgcgcc cgctcgcag ggttttcct gataagact aagtttttt ggcagtgtt acgagcaag taccgccaa attggcaac gcggtgccg ccgcccctc gctaaggcc atcgggctc gagatcaag agagccctg cggaaccgc caccgctaa
- >TC031507 RA
ttcatacat gttgatttt atttttaaa aatactact aactcgaga agttttgtt ttgtctagt ctagtctct agttattcg ctaaattct tcaataatg tccttaaga aaaaggttt agagagtct cccgcacag gaaacgggg gacaacacg aaaaaacac aaacgaacc tctactcat tcagacaag tgcaaattt tgcgcacaa actcttaat caggcagta gtttacaca aatttcccc gatgactat gccgaggag tctgttgct ttaacatcc gaaagactg aatgtgtac aacggtacc gaaaacatc agttttgag gagggcgac ttgcccagt cacaaaata acttgtttc agtatttac gacttgaat gaccacttg tgccccctt gacaccggg ttgttgcaa aaagaggtc tatttgcat atcgggggt tacattaaa ccaattttc gacgacgac ccgagcccc gaaaatggc attgcaacg cttgacatg gggcccatt cacgagtgg ttcattgcc ggttttgac gggggcgag aaagtctta ctggggatt atcactaac tacgcccag tacgtttta atggagccc tcgctggaa tacgccccc attttcaaa agcctccaa gagaaaacc gttttgttt aaattcgtt attgagttc ctactaagc aatttcagc cgcgctcct tcctacgag gagctactg gccgcagtg gaaaacagc gataaccca ctgttgacc gaggaattt ctcctgcaa aacgcccag tttgtgtgc gatcagatc cacagcttc gacttggac gctggggac aacaacgac atgccgtta ctcacaatg ccatgcgtt cgaactttg gcgagtttg gctggtgtt agcttccgg aagcgccaa ctcatgcgg aatctttta gggaaccaa aggagaact aagctccag ttgagcaaa gccacagcc acgccgcta gtccgccaa ttgttcgaa cagttgttc ccgaatcaa atccagact gaaaattgc cagtcgccc aaaaagaca cggtgtggc atttgcgac acgtgtcgg tcgcccgac tgcggccag tgcgtttac tgcaaagac atggtcaag ttcggcggt gatggcaac atgaaacag ccctgcaag ctgagacgt tgccccaac ttggtcgtg caagagttg gacgagagt gacgacgaa actgtcgtg acaccagcc agggggcgg gaggacggc cgcagacct ggcgtgagc cgggtcctg agcgacggc ccccttcag aatttccgc gaatcgatt gatatagtt cttggtggg gttaatgat gacgacacg ggcatttta ctcaacatc aagaccggc gattttgtc cagttatcg cccaaaagt gcaaccaaa ccgaacaca atcgcccga gttgttaac atatacacg gctacggaa ccaatggtc catgtgtat ttgttctac cgcggcaat gaaacgatt ttaggggaa gtagccaat ccgcaagaa cttttcgct tcaaatgaa tgtgaggat tgcccggtt gccgccata gtaggacag gccaaggtt gtttataaa gcaacgccc gacaattgg gccgatttg ggcgggatt gagtgtttg ccatctggc gatgatagg tttttttac gcaaaacag tacgacagt gagactgcc acgttcacg gactatgtt aaagtcagg aatgagttg gattcgtgc ggacattgc aggttgaac gaggaacga aagaaaatt gaaactccc actttcaaa gataacaaa gtccaatgg cgcaacgag ttttacgaa gcagggaac tcggttttt ttggatcct tcggtttat caatttccg attcccgtt gagtgcact ttcgatgat gcgaaagag atgaatgag agcaaatac ccggagtac taccgaaaa cgcgaagct aacgcttct tgcaaaaaa atgccccaa ccgttctgc attggatta atcgagacc ttatgcgat gggcccgcc ggggtattg atggcgatt cgggtgttt tttcggcct gaaaacaca aagggtggc agcatgtta tcgtatcag tctgacatc aatctgctg ttttggtcc actaaattt ataacagtt ccgtttcgt aaagtgatg gggaagtgc tatgttgtt tactgttct gacgaacag aaggcgcgg gaatggtcc gaggggggc ccttatcgc tactatttc agacagcaa tacgatcca gactgtggt gaattagtt ccacccacg tatagctgc acaagactg gctttgaga gagggagct ggccaagac gaacgtcct tttggcagg ccgctgaaa tgcctggac gttttcgcg ggctgtggg ggactgtca cagggcttt cacgctgca ggcgtcgca aacaccaaa tgggccatt gagaacgac aaacccgca ctggacacg ttccgtcat aacaatcgg acttgccat gtttttcga gacgattgt aacgtgctt ttacggaat gtcatgtct ggaaaaggg ggcctgccc cccaagagc gaggttgaa atgatcgtg ggcgggccc ccatgccag gggttttct gggatgaac cgattcaac gaaggcgaa tactcgttg tttaaaaac tcgctcgta gtttcgctt ctaagtttg tgcgattac taccgaccc ttgatattc gtattggag aacgtgcgt aatttcatg ctgtacaag ggcgggctt attttgaag ctgacgctc caatgtctc gtggccatc ggctaccag gtgcgaatt tcaatcgtg gaagcgggc gaattcggc gtgccccag gcccgcagg cgctttatc ctaatagcg tcagccccc gggtatcaa ttgccccgc attccggag cctcaacac gtttttctc cagagaggc tcgcgtcta gatatttac gtagacggt gttaaatac acgaacggt aatttctgg acccagtca gcgccttat cgcatgctt cacgtgcgt gatgccatc gctgatctg ccagccatt gaacacgat gataaccgc ccccaaatg ccgtatgac gacgacgaa ggaacgtca catttccaa aggaaaatg cgcggtaat caggaggag ctactggac catatttgc aaaccgatc gcccccatt gtgcaaact agaatcaag ctaattccg ccaaaaggc ggtgccgac tggaggaat ttgccaaat gtgcgggtg caactcccg gatgggacc atgactgag gtgctccag taccggtac aggaccaag aagcagaaa gacggggag ccgaaccgc ggcgtttgc gcgtgttcg gttgaaaag gcgtgcgat ccgaatgat cgccaatca aatactttg attccgtgg tgtttgcca cacactgcc gataggcac aataactgg gccggggtg tacggccgg cttgactgg agcgggtac ttcgccacg accaccact aaccccgaa cccatgggg aaacaaggg cgagtgata cacccggac caaaaccga ctgataagc gtgcgggaa tgcgcccgc tcgcagggt tttcctgat aagactaag ttttttggc agtgttacg agcaagtac cgccaaatt ggcaacgcg gtgccgccg cccctcgct aaggccatc gggctcgag atcaagaga gccctgcgg aaccgccac cgctaaatg ttgttccta gttgtgttc tgttcagtt tatttgtat ttttataaa gtgttttga atgatctg
