Status
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Gene information
General information
TC031507
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013587
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3397535..3402462
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031507
    iBeetle screen iB_08496

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031507 RA
      MSLRKRFRE SPAQETGDN TKKHKRTST HSDKCKFCA 
      QTLNQAVVY TNFPDDYAE ESVALTSER LNVYNGTEN 
      ISFEEGDLP SHKITCFSI YDLNDHLCP LDTGLLQKE 
      VYLHIGGYI KPIFDDDPS PENGIATLD MGPIHEWFI 
      AGFDGGEKV LLGIITNYA QYVLMEPSL EYAPIFKSL 
      QEKTVLFKF VIEFLLSNF SRAPSYEEL LAAVENSDN 
      PLLTEEFLL QNAQFVCDQ IHSFDLDAG DNNDMPLLT 
      MPCVRTLAS LAGVSFRKR QLMRNLLGN QRRTKLQLS 
      KATATPLVR QLFEQLFPN QIQTENCQS PKKTRCGIC 
      DTCRSPDCG QCVYCKDMV KFGGDGNMK QPCKLRRCP 
      NLVVQELDE SDDETVVTP ARGREDGRR PGVSRVLSD 
      GPLQNFRES IDIVLGGVN DDDTGILLN IKTGDFVQL 
      SPKSATKPN TIARVVNIY TATEPMVHV YLFYRGNET 
      ILGEVANPQ ELFASNECE DCPVAAIVG QAKVVYKAT 
      PDNWADLGG IECLPSGDD RFFYAKQYD SETATFTDY 
      VKVRNELDS CGHCRLNEE RKKIETPTF KDNKVQWRN 
      EFYEAGNSV FLDPSVYQF PIPVECTFD DAKEMNESK 
      YPEYYRKRE ANASCKKMP QPFCIGLIE TLCDGPAGV 
      LMAIRVFFR PENTKGGSM LSYQSDINL LFWSTKFIT 
      VPFRKVMGK CYVVYCSDE QKAREWSEG GPYRYYFRQ 
      QYDPDCGEL VPPTYSCTR LALREGAGQ DERPFGRPL 
      KCLDVFAGC GGLSQGFHA AGVANTKWA IENDKPALD 
      TFRHNNRTC HVFRDDCNV LLRNVMSGK GGLPPKSEV 
      EMIVGGPPC QGFSGMNRF NEGEYSLFK NSLVVSLLS 
      LCDYYRPLI FVLENVRNF MLYKGGLIL KLTLQCLVA 
      IGYQVRISI VEAGEFGVP QARRRFILI ASAPGYQLP 
      RIPEPQHVF LQRGSRLDI YVDGVKYTN GNFWTQSAP 
      YRMLHVRDA IADLPAIEH DDNRPQMPY DDDEGTSHF 
      QRKMRGNQE ELLDHICKP IAPIVQTRI KLIPPKGGA 
      DWRNLPNVR VQLPDGTMT EVLQYRYRT KKQKDGEPN 
      RGVCACSVE KACDPNDRQ SNTLIPWCL PHTADRHNN 
      WAGVYGRLD WSGYFATTT TNPEPMGKQ GRVIHPDQN 
      RLISVRECA RSQGFPDKT KFFGSVTSK YRQIGNAVP 
      PPLAKAIGL EIKRALRNR HR
    CDS sequence
    • >TC031507 RA
      atgtcctta agaaaaagg tttagagag tctcccgca 
      caggaaacg ggggacaac acgaaaaaa cacaaacga 
      acctctact cattcagac aagtgcaaa ttttgcgca 
      caaactctt aatcaggca gtagtttac acaaatttc 
      cccgatgac tatgccgag gagtctgtt gctttaaca 
      tccgaaaga ctgaatgtg tacaacggt accgaaaac 
      atcagtttt gaggagggc gacttgccc agtcacaaa 
      ataacttgt ttcagtatt tacgacttg aatgaccac 
      ttgtgcccc cttgacacc gggttgttg caaaaagag 
      gtctatttg catatcggg ggttacatt aaaccaatt 
      ttcgacgac gacccgagc cccgaaaat ggcattgca 
      acgcttgac atggggccc attcacgag tggttcatt 
      gccggtttt gacgggggc gagaaagtc ttactgggg 
      attatcact aactacgcc cagtacgtt ttaatggag 
      ccctcgctg gaatacgcc cccattttc aaaagcctc 
      caagagaaa accgttttg tttaaattc gttattgag 
      ttcctacta agcaatttc agccgcgct ccttcctac 
      gaggagcta ctggccgca gtggaaaac agcgataac 
      ccactgttg accgaggaa tttctcctg caaaacgcc 
      cagtttgtg tgcgatcag atccacagc ttcgacttg 
      gacgctggg gacaacaac gacatgccg ttactcaca 
      atgccatgc gttcgaact ttggcgagt ttggctggt 
      gttagcttc cggaagcgc caactcatg cggaatctt 
      ttagggaac caaaggaga actaagctc cagttgagc 
      aaagccaca gccacgccg ctagtccgc caattgttc 
      gaacagttg ttcccgaat caaatccag actgaaaat 
      tgccagtcg cccaaaaag acacggtgt ggcatttgc 
      gacacgtgt cggtcgccc gactgcggc cagtgcgtt 
      tactgcaaa gacatggtc aagttcggc ggtgatggc 
      aacatgaaa cagccctgc aagctgaga cgttgcccc 
      aacttggtc gtgcaagag ttggacgag agtgacgac 
      gaaactgtc gtgacacca gccaggggg cgggaggac 
      ggccgcaga cctggcgtg agccgggtc ctgagcgac 
      ggccccctt cagaatttc cgcgaatcg attgatata 
      gttcttggt ggggttaat gatgacgac acgggcatt 
      ttactcaac atcaagacc ggcgatttt gtccagtta 
      tcgcccaaa agtgcaacc aaaccgaac acaatcgcc 
      cgagttgtt aacatatac acggctacg gaaccaatg 
      gtccatgtg tatttgttc taccgcggc aatgaaacg 
      attttaggg gaagtagcc aatccgcaa gaacttttc 
      gcttcaaat gaatgtgag gattgcccg gttgccgcc 
      atagtagga caggccaag gttgtttat aaagcaacg 
      cccgacaat tgggccgat ttgggcggg attgagtgt 
      ttgccatct ggcgatgat aggtttttt tacgcaaaa 
      cagtacgac agtgagact gccacgttc acggactat 
      gttaaagtc aggaatgag ttggattcg tgcggacat 
      tgcaggttg aacgaggaa cgaaagaaa attgaaact 
      cccactttc aaagataac aaagtccaa tggcgcaac 
      gagttttac gaagcaggg aactcggtt tttttggat 
      ccttcggtt tatcaattt ccgattccc gttgagtgc 
      actttcgat gatgcgaaa gagatgaat gagagcaaa 
      tacccggag tactaccga aaacgcgaa gctaacgct 
      tcttgcaaa aaaatgccc caaccgttc tgcattgga 
      ttaatcgag accttatgc gatgggccc gccggggta 
      ttgatggcg attcgggtg ttttttcgg cctgaaaac 
      acaaagggt ggcagcatg ttatcgtat cagtctgac 
      atcaatctg ctgttttgg tccactaaa tttataaca 
      gttccgttt cgtaaagtg atggggaag tgctatgtt 
      gtttactgt tctgacgaa cagaaggcg cgggaatgg 
      tccgagggg ggcccttat cgctactat ttcagacag 
      caatacgat ccagactgt ggtgaatta gttccaccc 
      acgtatagc tgcacaaga ctggctttg agagaggga 
      gctggccaa gacgaacgt ccttttggc aggccgctg 
      aaatgcctg gacgttttc gcgggctgt gggggactg 
      tcacagggc tttcacgct gcaggcgtc gcaaacacc 
      aaatgggcc attgagaac gacaaaccc gcactggac 
      acgttccgt cataacaat cggacttgc catgttttt 
      cgagacgat tgtaacgtg cttttacgg aatgtcatg 
      tctggaaaa gggggcctg ccccccaag agcgaggtt 
      gaaatgatc gtgggcggg cccccatgc caggggttt 
      tctgggatg aaccgattc aacgaaggc gaatactcg 
      ttgtttaaa aactcgctc gtagtttcg cttctaagt 
      ttgtgcgat tactaccga cccttgata ttcgtattg 
      gagaacgtg cgtaatttc atgctgtac aagggcggg 
      cttattttg aagctgacg ctccaatgt ctcgtggcc 
      atcggctac caggtgcga atttcaatc gtggaagcg 
      ggcgaattc ggcgtgccc caggcccgc aggcgcttt 
      atcctaata gcgtcagcc cccgggtat caattgccc 
      cgcattccg gagcctcaa cacgttttt ctccagaga 
      ggctcgcgt ctagatatt tacgtagac ggtgttaaa 
      tacacgaac ggtaatttc tggacccag tcagcgcct 
      tatcgcatg cttcacgtg cgtgatgcc atcgctgat 
      ctgccagcc attgaacac gatgataac cgcccccaa 
      atgccgtat gacgacgac gaaggaacg tcacatttc 
      caaaggaaa atgcgcggt aatcaggag gagctactg 
      gaccatatt tgcaaaccg atcgccccc attgtgcaa 
      actagaatc aagctaatt ccgccaaaa ggcggtgcc 
      gactggagg aatttgcca aatgtgcgg gtgcaactc 
      ccggatggg accatgact gaggtgctc cagtaccgg 
      tacaggacc aagaagcag aaagacggg gagccgaac 
      cgcggcgtt tgcgcgtgt tcggttgaa aaggcgtgc 
      gatccgaat gatcgccaa tcaaatact ttgattccg 
      tggtgtttg ccacacact gccgatagg cacaataac 
      tgggccggg gtgtacggc cggcttgac tggagcggg 
      tacttcgcc acgaccacc actaacccc gaacccatg 
      gggaaacaa gggcgagtg atacacccg gaccaaaac 
      cgactgata agcgtgcgg gaatgcgcc cgctcgcag 
      ggttttcct gataagact aagtttttt ggcagtgtt 
      acgagcaag taccgccaa attggcaac gcggtgccg 
      ccgcccctc gctaaggcc atcgggctc gagatcaag 
      agagccctg cggaaccgc caccgctaa
    mRNA sequence
    • >TC031507 RA
      ttcatacat gttgatttt atttttaaa aatactact 
      aactcgaga agttttgtt ttgtctagt ctagtctct 
      agttattcg ctaaattct tcaataatg tccttaaga 
      aaaaggttt agagagtct cccgcacag gaaacgggg 
      gacaacacg aaaaaacac aaacgaacc tctactcat 
      tcagacaag tgcaaattt tgcgcacaa actcttaat 
      caggcagta gtttacaca aatttcccc gatgactat 
      gccgaggag tctgttgct ttaacatcc gaaagactg 
      aatgtgtac aacggtacc gaaaacatc agttttgag 
      gagggcgac ttgcccagt cacaaaata acttgtttc 
      agtatttac gacttgaat gaccacttg tgccccctt 
      gacaccggg ttgttgcaa aaagaggtc tatttgcat 
      atcgggggt tacattaaa ccaattttc gacgacgac 
      ccgagcccc gaaaatggc attgcaacg cttgacatg 
      gggcccatt cacgagtgg ttcattgcc ggttttgac 
      gggggcgag aaagtctta ctggggatt atcactaac 
      tacgcccag tacgtttta atggagccc tcgctggaa 
      tacgccccc attttcaaa agcctccaa gagaaaacc 
      gttttgttt aaattcgtt attgagttc ctactaagc 
      aatttcagc cgcgctcct tcctacgag gagctactg 
      gccgcagtg gaaaacagc gataaccca ctgttgacc 
      gaggaattt ctcctgcaa aacgcccag tttgtgtgc 
      gatcagatc cacagcttc gacttggac gctggggac 
      aacaacgac atgccgtta ctcacaatg ccatgcgtt 
      cgaactttg gcgagtttg gctggtgtt agcttccgg 
      aagcgccaa ctcatgcgg aatctttta gggaaccaa 
      aggagaact aagctccag ttgagcaaa gccacagcc 
      acgccgcta gtccgccaa ttgttcgaa cagttgttc 
      ccgaatcaa atccagact gaaaattgc cagtcgccc 
      aaaaagaca cggtgtggc atttgcgac acgtgtcgg 
      tcgcccgac tgcggccag tgcgtttac tgcaaagac 
      atggtcaag ttcggcggt gatggcaac atgaaacag 
      ccctgcaag ctgagacgt tgccccaac ttggtcgtg 
      caagagttg gacgagagt gacgacgaa actgtcgtg 
      acaccagcc agggggcgg gaggacggc cgcagacct 
      ggcgtgagc cgggtcctg agcgacggc ccccttcag 
      aatttccgc gaatcgatt gatatagtt cttggtggg 
      gttaatgat gacgacacg ggcatttta ctcaacatc 
      aagaccggc gattttgtc cagttatcg cccaaaagt 
      gcaaccaaa ccgaacaca atcgcccga gttgttaac 
      atatacacg gctacggaa ccaatggtc catgtgtat 
      ttgttctac cgcggcaat gaaacgatt ttaggggaa 
      gtagccaat ccgcaagaa cttttcgct tcaaatgaa 
      tgtgaggat tgcccggtt gccgccata gtaggacag 
      gccaaggtt gtttataaa gcaacgccc gacaattgg 
      gccgatttg ggcgggatt gagtgtttg ccatctggc 
      gatgatagg tttttttac gcaaaacag tacgacagt 
      gagactgcc acgttcacg gactatgtt aaagtcagg 
      aatgagttg gattcgtgc ggacattgc aggttgaac 
      gaggaacga aagaaaatt gaaactccc actttcaaa 
      gataacaaa gtccaatgg cgcaacgag ttttacgaa 
      gcagggaac tcggttttt ttggatcct tcggtttat 
      caatttccg attcccgtt gagtgcact ttcgatgat 
      gcgaaagag atgaatgag agcaaatac ccggagtac 
      taccgaaaa cgcgaagct aacgcttct tgcaaaaaa 
      atgccccaa ccgttctgc attggatta atcgagacc 
      ttatgcgat gggcccgcc ggggtattg atggcgatt 
      cgggtgttt tttcggcct gaaaacaca aagggtggc 
      agcatgtta tcgtatcag tctgacatc aatctgctg 
      ttttggtcc actaaattt ataacagtt ccgtttcgt 
      aaagtgatg gggaagtgc tatgttgtt tactgttct 
      gacgaacag aaggcgcgg gaatggtcc gaggggggc 
      ccttatcgc tactatttc agacagcaa tacgatcca 
      gactgtggt gaattagtt ccacccacg tatagctgc 
      acaagactg gctttgaga gagggagct ggccaagac 
      gaacgtcct tttggcagg ccgctgaaa tgcctggac 
      gttttcgcg ggctgtggg ggactgtca cagggcttt 
      cacgctgca ggcgtcgca aacaccaaa tgggccatt 
      gagaacgac aaacccgca ctggacacg ttccgtcat 
      aacaatcgg acttgccat gtttttcga gacgattgt 
      aacgtgctt ttacggaat gtcatgtct ggaaaaggg 
      ggcctgccc cccaagagc gaggttgaa atgatcgtg 
      ggcgggccc ccatgccag gggttttct gggatgaac 
      cgattcaac gaaggcgaa tactcgttg tttaaaaac 
      tcgctcgta gtttcgctt ctaagtttg tgcgattac 
      taccgaccc ttgatattc gtattggag aacgtgcgt 
      aatttcatg ctgtacaag ggcgggctt attttgaag 
      ctgacgctc caatgtctc gtggccatc ggctaccag 
      gtgcgaatt tcaatcgtg gaagcgggc gaattcggc 
      gtgccccag gcccgcagg cgctttatc ctaatagcg 
      tcagccccc gggtatcaa ttgccccgc attccggag 
      cctcaacac gtttttctc cagagaggc tcgcgtcta 
      gatatttac gtagacggt gttaaatac acgaacggt 
      aatttctgg acccagtca gcgccttat cgcatgctt 
      cacgtgcgt gatgccatc gctgatctg ccagccatt 
      gaacacgat gataaccgc ccccaaatg ccgtatgac 
      gacgacgaa ggaacgtca catttccaa aggaaaatg 
      cgcggtaat caggaggag ctactggac catatttgc 
      aaaccgatc gcccccatt gtgcaaact agaatcaag 
      ctaattccg ccaaaaggc ggtgccgac tggaggaat 
      ttgccaaat gtgcgggtg caactcccg gatgggacc 
      atgactgag gtgctccag taccggtac aggaccaag 
      aagcagaaa gacggggag ccgaaccgc ggcgtttgc 
      gcgtgttcg gttgaaaag gcgtgcgat ccgaatgat 
      cgccaatca aatactttg attccgtgg tgtttgcca 
      cacactgcc gataggcac aataactgg gccggggtg 
      tacggccgg cttgactgg agcgggtac ttcgccacg 
      accaccact aaccccgaa cccatgggg aaacaaggg 
      cgagtgata cacccggac caaaaccga ctgataagc 
      gtgcgggaa tgcgcccgc tcgcagggt tttcctgat 
      aagactaag ttttttggc agtgttacg agcaagtac 
      cgccaaatt ggcaacgcg gtgccgccg cccctcgct 
      aaggccatc gggctcgag atcaagaga gccctgcgg 
      aaccgccac cgctaaatg ttgttccta gttgtgttc 
      tgttcagtt tatttgtat ttttataaa gtgttttga 
      atgatctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY