| General information | |||
| TC033341
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9555078..9561395 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033341 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08488 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033341 RA
MLNEAEEEL ELELPKKKP WRALISFWI FGLCNNYGY VVMLTAAAD IIFTSGGKR SKERECTYV STGTILLAD IIPGIFAKL VTPFVPFCV RVRIVICVL LSASGFLFV GCGRILTLS IIGVVATSF SSGLGEVTF LQYSSYYDS NVLSTWSSG TGGAGVIGA LSYALLQNI GFTLTLMIM LLIPSTMAL TFFMLLPKP QPTETAVIQ QDVNAEEIK KPKETLIKK IMHIPSLCK YMIPFGLVY FLEYFINQG TFELIHFKN TRLSPDDQY RWLQVIYQV GVFISRSSA NLFYIKQIW IMSVLQAVN VAIFTTEVI YFYLPNFWI VVALTLWEG LLGGAAYVN TYYRIRNEV KEEMHQFSM AITSFSDSI GISLAGIVA VYAHNAICD LPMPKHSIC TRKKEVLKT KLNHHYVTY QRTEFYKCG FLYLKKCRR YKTECCHDN TYITHFTDY ETEKYCCDG CDVRTFVQM LQANVVYVF TTIIIILVI VFLFLQIRK CRKKNCDVV DESLELTKK MTPKNSETE FSKIELEEF DEPPNVSKL AEDLLIAVQ KNAIPEIKH LVSQNRSIL SYEYPFYEH QTILLLACN DSHNMCSVT REVIETLIE LGANCNEPS RNDHWEPLH YTALNANKT KMQTILPHS QINSLVYCS KQTTKKYDF CPTIKDSYS NNALNVLLK CGNRQKQFV QCCQLLIEN GINVNQTDS NGVSPSDLI WKMDNSELK QLLRDKTSP KIVDNTFGN IKLSKIQRF LSLDLSHED VDRVDGADC PTSSCTILQ LCCAKGLTS CVVHLLEKG ANPNKTIPK NPNLPVMIA VNSDHKEIV EILLQKNAD LPNNVLLHL QQLHRDDDT LVLADRYLK IILRHLARF EATTVQKYL SCKDEQGRS ALHYAISYD CRENILALL ALGASLVEK DDFGNKLLE SIEPKTLET FFENNCKVA QSRTNGESK FTVTIDYKS LVAETSPDS DFLHTMTKI PELNYLTNH PVVALYLAM KWTKCQWNL SVAFSLVFL TSTLGIFMQ LGNFSFFTV KVIILQEIT ITFFKYIAF YSFPLVAFF FCFYMLNDD KDYLFFPML YETVTMFTG DFDADYPMH FKRNPIFGH LIYVVFVIL IGIILHNLL IGLAVNDLQ AICYEAKFI DKRERSKYI TNVENVLFT KLQKSYYFR PMFENVLNF CRVFDNFDY TLTVYSDSK TFYLEKMGK KIVIRDQNI ITFLQNGIK DGSLKNKST EDSYSLKSL HKKIEELEK LIKNLNFSE TKS
- >TC033341 RA
atgctaaat gaagcagaa gaggaactg gaactcgag ctccctaaa aaaaagcca tggcgggct ttaatttcc ttctggatt tttggactg tgtaacaat tacggatac gtggtcatg ttaacagct gcagctgat ataatattc acaagtggg ggaaaaagg agcaaggaa agggaatgc acttatgta tccacaggg acgatatta ctagccgat ataattccg ggaattttt gcaaaattg gtaacgccc tttgtccct ttttgcgtg agagtacga attgtgatt tgtgtgttg ttgtcagct tctggattt ctttttgtg ggttgtgga cgaattttg acattgtct attattgga gttgttgca acgtccttt tcttcagga cttggtgaa gtgactttt cttcaatat agttcgtat tacgacagt aatgtgctc tccacttgg agttcaggc actggaggg gctggagtt ataggcgcc ctttcgtac gcgcttttg caaaacatt ggctttacg ttaactttg atgataatg ctcctgatt ccgtcaaca atggctttg acttttttc atgcttttg ccgaaaccg cagccaact gagactgcg gtaatccag caagacgtt aatgccgaa gagatcaaa aaacccaaa gagacacta attaagaaa atcatgcac attccctct ctctgcaag tacatgata ccgttcggt ttggtttat tttctggaa tattttata aatcaagga actttcgag ttgattcat tttaaaaat acgcgttta agtcccgat gatcagtac agatggctt caagttatc taccaagtc ggtgttttt atttcacga tcttcggcc aatttattt tatataaaa caaatttgg ataatgtcg gtgttacaa gctgttaat gtagcaatt tttacaact gaagtcatt tatttttat ttgccgaat ttttggatt gttgttgct ttaactctg tgggaaggt ctcttaggt ggggccgct tatgttaat acatattat cgaattcgt aatgaggtc aaagaggaa atgcatcaa ttttcaatg gcaattacg agtttttcc gatagcatt ggaatttct ctagctgga attgtggca gtgtatgca cataatgcc atttgcgac ctcccaatg ccaaaacat agtatttgt acacgaaaa aaagaggtc cttaagaca aaattaaat catcattat gtaacgtat caacgcacc gaattttac aaatgtgga tttctttat ttaaaaaaa tgtaggaga tataaaacc gaatgctgc catgacaac acatacatc acacatttc actgactat gaaactgag aaatattgc tgcgatggt tgcgatgtt cgcactttt gtgcaaatg ttacaagca aatgttgtg tatgtgttt acgacaatt attattatt cttgtgata gtttttcta tttcttcaa atcagaaaa tgtaggaag aaaaactgt gatgttgta gatgaaagt ttggaatta acaaaaaaa atgactcct aaaaattcg gaaaccgaa ttctctaaa atagaactt gaggaattt gacgaacca ccaaatgtt tccaaacta gccgaagat cttttaatc gcagtccaa aaaaacgcc attcccgaa attaaacac ctagtttcg caaaatcgg agtatccta tcatacgaa tacccattt tacgagcac caaaccatc ctactactg gcctgtaac gactcacac aacatgtgc tcagtgaca cgcgaagtc atcgaaact ttaatcgaa ctcggagcg aattgtaac gaacccagc agaaacgac cactgggaa cccctccat tacaccgcc cttaacgcc aacaaaacc aaaatgcaa acaattctc ccacacagc caaatcaac tcgctggtc tactgctcc aaacaaacc accaaaaag tacgacttt tgccccacc atcaaggac tcgtactca aacaatgcc ctgaacgtt ctcctgaaa tgcggcaac cgccagaaa caattcgtc caatgctgc caacttctg attgaaaac gggatcaac gtaaaccag accgatagc aacggcgtg tccccgtct gatttaatc tggaaaatg gacaacagc gaacttaaa caactactc agagataag acaagcccg aaaatcgtt gataacacc ttcgggaac ataaaactg agcaaaatc cagcgcttc ctctccttg gacttgtca cacgaagac gtggacaga gtagacggt gccgattgt ccaacttcc agctgcaca atcctgcag ttgtgctgc gccaaagga ctgacttcg tgtgtcgtc caccttttg gaaaaaggc gcaaacccg aataaaacc atcccgaag aaccccaac ttgccggtc atgatcgcc gtcaattcc gaccacaag gaaatcgtc gaaatactc ctccagaag aacgcggat ttgcccaac aatgtgtta ctccacttg cagcaattg caccgagat gacgacact ctggtcctg gctgataga tacttaaaa ataattttg agacacttg gctcggttt gaagccaca actgtccag aaatattta agttgtaag gacgaacag ggtcggagt gctttacac tacgccatt tcctacgac tgtcgtgag aatatttta gcgctcctg gcacttgga gcctccttg gtggagaaa gacgacttc gggaacaaa ttacttgag tcaattgag cccaaaact ttggagact tttttcgag aataattgc aaagtagcg caaagtcgc acaaatggg gagagtaag tttacagtg accattgat tacaagtct ctggttgcg gaaacaagc cctgatagt gattttctc cacactatg acgaaaatt cccgagttg aactacttg accaatcac cccgtggtg gcgctttat ttggccatg aaatggacc aaatgtcag tggaactta tcagtggcg ttttcccta gttttcctc acttccacc cttggcatt ttcatgcag ttgggcaat ttctcgttt ttcacagtc aaggttatc attttgcaa gaaatcacg atcacgttt ttcaaatat atagcgttt tatagtttt cccttggtg gcgtttttt ttctgcttt tacatgctg aacgatgac aaagactat ttgtttttc ccaatgctt tacgaaact gtgaccatg ttcactggg gattttgac gccgattat ccaatgcac ttcaaacga aacccaatt ttcgggcac ttgatttac gtggttttc gtcatcttg attgggatt attttgcac aacttgctg attggtcta gcggtcaat gatttgcaa gcgatttgt tacgaagcg aagtttatc gataagagg gaacgctcg aaatatata acaaatgtt gaaaatgtt ctctttacc aagttgcaa aagtcctat tattttaga ccgatgttt gaaaacgtg ctgaatttt tgtcgcgtg ttcgataat tttgactac accctaacg gtttattca gactcgaag acgttttat ttggagaaa atggggaaa aaaattgtg attcgtgat caaaatata atcacgttt ttgcaaaat gggattaag gatggctct cttaaaaat aaaagtact gaagattcg tactcgttg aagagcttg cataagaaa attgaagag ttggaaaaa ctaatcaaa aatttaaat ttttccgaa acaaaatca taa
- >TC033341 RA
ataaatgcg tcctacaaa tgctaaatg aagcagaag aggaactgg aactcgagc tccctaaaa aaaagccat ggcgggctt taatttcct tctggattt ttggactgt gtaacaatt acggatacg tggtcatgt taacagctg cagctgata taatattca caagtgggg gaaaaagga gcaaggaaa gggaatgca cttatgtat ccacaggga cgatattac tagccgata taattccgg gaatttttg caaaattgg taacgccct ttgtccctt tttgcgtga gagtacgaa ttgtgattt gtgtgttgt tgtcagctt ctggatttc tttttgtgg gttgtggac gaattttga cattgtcta ttattggag ttgttgcaa cgtcctttt cttcaggac ttggtgaag tgacttttc ttcaatata gttcgtatt acgacagta atgtgctct ccacttgga gttcaggca ctggagggg ctggagtta taggcgccc tttcgtacg cgcttttgc aaaacattg gctttacgt taactttga tgataatgc tcctgattc cgtcaacaa tggctttga cttttttca tgcttttgc cgaaaccgc agccaactg agactgcgg taatccagc aagacgtta atgccgaag agatcaaaa aacccaaag agacactaa ttaagaaaa tcatgcaca ttccctctc tctgcaagt acatgatac cgttcggtt tggtttatt ttctggaat attttataa atcaaggaa ctttcgagt tgattcatt ttaaaaata cgcgtttaa gtcccgatg atcagtaca gatggcttc aagttatct accaagtcg gtgttttta tttcacgat cttcggcca atttatttt atataaaac aaatttgga taatgtcgg tgttacaag ctgttaatg tagcaattt ttacaactg aagtcattt atttttatt tgccgaatt tttggattg ttgttgctt taactctgt gggaaggtc tcttaggtg gggccgctt atgttaata catattatc gaattcgta atgaggtca aagaggaaa tgcatcaat tttcaatgg caattacga gtttttccg atagcattg gaatttctc tagctggaa ttgtggcag tgtatgcac ataatgcca tttgcgacc tcccaatgc caaaacata gtatttgta cacgaaaaa aagaggtcc ttaagacaa aattaaatc atcattatg taacgtatc aacgcaccg aattttaca aatgtggat ttctttatt taaaaaaat gtaggagat ataaaaccg aatgctgcc atgacaaca catacatca cacatttca ctgactatg aaactgaga aatattgct gcgatggtt gcgatgttc gcacttttg tgcaaatgt tacaagcaa atgttgtgt atgtgttta cgacaatta ttattattc ttgtgatag tttttctat ttcttcaaa tcagaaaat gtaggaaga aaaactgtg atgttgtag atgaaagtt tggaattaa caaaaaaaa tgactccta aaaattcgg aaaccgaat tctctaaaa tagaacttg aggaatttg acgaaccac caaatgttt ccaaactag ccgaagatc ttttaatcg cagtccaaa aaaacgcca ttcccgaaa ttaaacacc tagtttcgc aaaatcgga gtatcctat catacgaat acccatttt acgagcacc aaaccatcc tactactgg cctgtaacg actcacaca acatgtgct cagtgacac gcgaagtca tcgaaactt taatcgaac tcggagcga attgtaacg aacccagca gaaacgacc actgggaac ccctccatt acaccgccc ttaacgcca acaaaacca aaatgcaaa caattctcc cacacagcc aaatcaact cgctggtct actgctcca aacaaacca ccaaaaagt acgactttt gccccacca tcaaggact cgtactcaa acaatgccc tgaacgttc tcctgaaat gcggcaacc gccagaaac aattcgtcc aatgctgcc aacttctga ttgaaaacg ggatcaacg taaaccaga ccgatagca acggcgtgt ccccgtctg atttaatct ggaaaatgg acaacagcg aacttaaac aactactca gagataaga caagcccga aaatcgttg ataacacct tcgggaaca taaaactga gcaaaatcc agcgcttcc tctccttgg acttgtcac acgaagacg tggacagag tagacggtg ccgattgtc caacttcca gctgcacaa tcctgcagt tgtgctgcg ccaaaggac tgacttcgt gtgtcgtcc accttttgg aaaaaggcg caaacccga ataaaacca tcccgaaga accccaact tgccggtca tgatcgccg tcaattccg accacaagg aaatcgtcg aaatactcc tccagaaga acgcggatt tgcccaaca atgtgttac tccacttgc agcaattgc accgagatg acgacactc tggtcctgg ctgatagat acttaaaaa taattttga gacacttgg ctcggtttg aagccacaa ctgtccaga aatatttaa gttgtaagg acgaacagg gtcggagtg ctttacact acgccattt cctacgact gtcgtgaga atattttag cgctcctgg cacttggag cctccttgg tggagaaag acgacttcg ggaacaaat tacttgagt caattgagc ccaaaactt tggagactt ttttcgaga ataattgca aagtagcgc aaagtcgca caaatgggg agagtaagt ttacagtga ccattgatt acaagtctc tggttgcgg aaacaagcc ctgatagtg attttctcc acactatga cgaaaattc ccgagttga actacttga ccaatcacc ccgtggtgg cgctttatt tggccatga aatggacca aatgtcagt ggaacttat cagtggcgt tttccctag ttttcctca cttccaccc ttggcattt tcatgcagt tgggcaatt tctcgtttt tcacagtca aggttatca ttttgcaag aaatcacga tcacgtttt tcaaatata tagcgtttt atagttttc ccttggtgg cgttttttt tctgctttt acatgctga acgatgaca aagactatt tgtttttcc caatgcttt acgaaactg tgaccatgt tcactgggg attttgacg ccgattatc caatgcact tcaaacgaa acccaattt tcgggcact tgatttacg tggttttcg tcatcttga ttgggatta ttttgcaca acttgctga ttggtctag cggtcaatg atttgcaag cgatttgtt acgaagcga agtttatcg ataagaggg aacgctcga aatatataa caaatgttg aaaatgttc tctttacca agttgcaaa agtcctatt attttagac cgatgtttg aaaacgtgc tgaattttt gtcgcgtgt tcgataatt ttgactaca ccctaacgg tttattcag actcgaaga cgttttatt tggagaaaa tggggaaaa aaattgtga ttcgtgatc aaaatataa tcacgtttt tgcaaaatg ggattaagg atggctctc ttaaaaata aaagtactg aagattcgt actcgttga agagcttgc ataagaaaa ttgaagagt tggaaaaac taatcaaaa atttaaatt tttccgaaa caaaatcat aacaatttt taccgcaat ttcgacaaa ccctttact ttttttcta aatataata ataaattga tattttgaa ccttaatga tatgttatt atgtcactt ccctaaatc accctccga taagtgaat aaataaata aacgtagct ttagtta
