Status
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Gene information
General information
TC033341
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015258,TC015259
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9555078..9561395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033341
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08488

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033341 RA
      MLNEAEEEL ELELPKKKP WRALISFWI FGLCNNYGY 
      VVMLTAAAD IIFTSGGKR SKERECTYV STGTILLAD 
      IIPGIFAKL VTPFVPFCV RVRIVICVL LSASGFLFV 
      GCGRILTLS IIGVVATSF SSGLGEVTF LQYSSYYDS 
      NVLSTWSSG TGGAGVIGA LSYALLQNI GFTLTLMIM 
      LLIPSTMAL TFFMLLPKP QPTETAVIQ QDVNAEEIK 
      KPKETLIKK IMHIPSLCK YMIPFGLVY FLEYFINQG 
      TFELIHFKN TRLSPDDQY RWLQVIYQV GVFISRSSA 
      NLFYIKQIW IMSVLQAVN VAIFTTEVI YFYLPNFWI 
      VVALTLWEG LLGGAAYVN TYYRIRNEV KEEMHQFSM 
      AITSFSDSI GISLAGIVA VYAHNAICD LPMPKHSIC 
      TRKKEVLKT KLNHHYVTY QRTEFYKCG FLYLKKCRR 
      YKTECCHDN TYITHFTDY ETEKYCCDG CDVRTFVQM 
      LQANVVYVF TTIIIILVI VFLFLQIRK CRKKNCDVV 
      DESLELTKK MTPKNSETE FSKIELEEF DEPPNVSKL 
      AEDLLIAVQ KNAIPEIKH LVSQNRSIL SYEYPFYEH 
      QTILLLACN DSHNMCSVT REVIETLIE LGANCNEPS 
      RNDHWEPLH YTALNANKT KMQTILPHS QINSLVYCS 
      KQTTKKYDF CPTIKDSYS NNALNVLLK CGNRQKQFV 
      QCCQLLIEN GINVNQTDS NGVSPSDLI WKMDNSELK 
      QLLRDKTSP KIVDNTFGN IKLSKIQRF LSLDLSHED 
      VDRVDGADC PTSSCTILQ LCCAKGLTS CVVHLLEKG 
      ANPNKTIPK NPNLPVMIA VNSDHKEIV EILLQKNAD 
      LPNNVLLHL QQLHRDDDT LVLADRYLK IILRHLARF 
      EATTVQKYL SCKDEQGRS ALHYAISYD CRENILALL 
      ALGASLVEK DDFGNKLLE SIEPKTLET FFENNCKVA 
      QSRTNGESK FTVTIDYKS LVAETSPDS DFLHTMTKI 
      PELNYLTNH PVVALYLAM KWTKCQWNL SVAFSLVFL 
      TSTLGIFMQ LGNFSFFTV KVIILQEIT ITFFKYIAF 
      YSFPLVAFF FCFYMLNDD KDYLFFPML YETVTMFTG 
      DFDADYPMH FKRNPIFGH LIYVVFVIL IGIILHNLL 
      IGLAVNDLQ AICYEAKFI DKRERSKYI TNVENVLFT 
      KLQKSYYFR PMFENVLNF CRVFDNFDY TLTVYSDSK 
      TFYLEKMGK KIVIRDQNI ITFLQNGIK DGSLKNKST 
      EDSYSLKSL HKKIEELEK LIKNLNFSE TKS
    CDS sequence
    • >TC033341 RA
      atgctaaat gaagcagaa gaggaactg gaactcgag 
      ctccctaaa aaaaagcca tggcgggct ttaatttcc 
      ttctggatt tttggactg tgtaacaat tacggatac 
      gtggtcatg ttaacagct gcagctgat ataatattc 
      acaagtggg ggaaaaagg agcaaggaa agggaatgc 
      acttatgta tccacaggg acgatatta ctagccgat 
      ataattccg ggaattttt gcaaaattg gtaacgccc 
      tttgtccct ttttgcgtg agagtacga attgtgatt 
      tgtgtgttg ttgtcagct tctggattt ctttttgtg 
      ggttgtgga cgaattttg acattgtct attattgga 
      gttgttgca acgtccttt tcttcagga cttggtgaa 
      gtgactttt cttcaatat agttcgtat tacgacagt 
      aatgtgctc tccacttgg agttcaggc actggaggg 
      gctggagtt ataggcgcc ctttcgtac gcgcttttg 
      caaaacatt ggctttacg ttaactttg atgataatg 
      ctcctgatt ccgtcaaca atggctttg acttttttc 
      atgcttttg ccgaaaccg cagccaact gagactgcg 
      gtaatccag caagacgtt aatgccgaa gagatcaaa 
      aaacccaaa gagacacta attaagaaa atcatgcac 
      attccctct ctctgcaag tacatgata ccgttcggt 
      ttggtttat tttctggaa tattttata aatcaagga 
      actttcgag ttgattcat tttaaaaat acgcgttta 
      agtcccgat gatcagtac agatggctt caagttatc 
      taccaagtc ggtgttttt atttcacga tcttcggcc 
      aatttattt tatataaaa caaatttgg ataatgtcg 
      gtgttacaa gctgttaat gtagcaatt tttacaact 
      gaagtcatt tatttttat ttgccgaat ttttggatt 
      gttgttgct ttaactctg tgggaaggt ctcttaggt 
      ggggccgct tatgttaat acatattat cgaattcgt 
      aatgaggtc aaagaggaa atgcatcaa ttttcaatg 
      gcaattacg agtttttcc gatagcatt ggaatttct 
      ctagctgga attgtggca gtgtatgca cataatgcc 
      atttgcgac ctcccaatg ccaaaacat agtatttgt 
      acacgaaaa aaagaggtc cttaagaca aaattaaat 
      catcattat gtaacgtat caacgcacc gaattttac 
      aaatgtgga tttctttat ttaaaaaaa tgtaggaga 
      tataaaacc gaatgctgc catgacaac acatacatc 
      acacatttc actgactat gaaactgag aaatattgc 
      tgcgatggt tgcgatgtt cgcactttt gtgcaaatg 
      ttacaagca aatgttgtg tatgtgttt acgacaatt 
      attattatt cttgtgata gtttttcta tttcttcaa 
      atcagaaaa tgtaggaag aaaaactgt gatgttgta 
      gatgaaagt ttggaatta acaaaaaaa atgactcct 
      aaaaattcg gaaaccgaa ttctctaaa atagaactt 
      gaggaattt gacgaacca ccaaatgtt tccaaacta 
      gccgaagat cttttaatc gcagtccaa aaaaacgcc 
      attcccgaa attaaacac ctagtttcg caaaatcgg 
      agtatccta tcatacgaa tacccattt tacgagcac 
      caaaccatc ctactactg gcctgtaac gactcacac 
      aacatgtgc tcagtgaca cgcgaagtc atcgaaact 
      ttaatcgaa ctcggagcg aattgtaac gaacccagc 
      agaaacgac cactgggaa cccctccat tacaccgcc 
      cttaacgcc aacaaaacc aaaatgcaa acaattctc 
      ccacacagc caaatcaac tcgctggtc tactgctcc 
      aaacaaacc accaaaaag tacgacttt tgccccacc 
      atcaaggac tcgtactca aacaatgcc ctgaacgtt 
      ctcctgaaa tgcggcaac cgccagaaa caattcgtc 
      caatgctgc caacttctg attgaaaac gggatcaac 
      gtaaaccag accgatagc aacggcgtg tccccgtct 
      gatttaatc tggaaaatg gacaacagc gaacttaaa 
      caactactc agagataag acaagcccg aaaatcgtt 
      gataacacc ttcgggaac ataaaactg agcaaaatc 
      cagcgcttc ctctccttg gacttgtca cacgaagac 
      gtggacaga gtagacggt gccgattgt ccaacttcc 
      agctgcaca atcctgcag ttgtgctgc gccaaagga 
      ctgacttcg tgtgtcgtc caccttttg gaaaaaggc 
      gcaaacccg aataaaacc atcccgaag aaccccaac 
      ttgccggtc atgatcgcc gtcaattcc gaccacaag 
      gaaatcgtc gaaatactc ctccagaag aacgcggat 
      ttgcccaac aatgtgtta ctccacttg cagcaattg 
      caccgagat gacgacact ctggtcctg gctgataga 
      tacttaaaa ataattttg agacacttg gctcggttt 
      gaagccaca actgtccag aaatattta agttgtaag 
      gacgaacag ggtcggagt gctttacac tacgccatt 
      tcctacgac tgtcgtgag aatatttta gcgctcctg 
      gcacttgga gcctccttg gtggagaaa gacgacttc 
      gggaacaaa ttacttgag tcaattgag cccaaaact 
      ttggagact tttttcgag aataattgc aaagtagcg 
      caaagtcgc acaaatggg gagagtaag tttacagtg 
      accattgat tacaagtct ctggttgcg gaaacaagc 
      cctgatagt gattttctc cacactatg acgaaaatt 
      cccgagttg aactacttg accaatcac cccgtggtg 
      gcgctttat ttggccatg aaatggacc aaatgtcag 
      tggaactta tcagtggcg ttttcccta gttttcctc 
      acttccacc cttggcatt ttcatgcag ttgggcaat 
      ttctcgttt ttcacagtc aaggttatc attttgcaa 
      gaaatcacg atcacgttt ttcaaatat atagcgttt 
      tatagtttt cccttggtg gcgtttttt ttctgcttt 
      tacatgctg aacgatgac aaagactat ttgtttttc 
      ccaatgctt tacgaaact gtgaccatg ttcactggg 
      gattttgac gccgattat ccaatgcac ttcaaacga 
      aacccaatt ttcgggcac ttgatttac gtggttttc 
      gtcatcttg attgggatt attttgcac aacttgctg 
      attggtcta gcggtcaat gatttgcaa gcgatttgt 
      tacgaagcg aagtttatc gataagagg gaacgctcg 
      aaatatata acaaatgtt gaaaatgtt ctctttacc 
      aagttgcaa aagtcctat tattttaga ccgatgttt 
      gaaaacgtg ctgaatttt tgtcgcgtg ttcgataat 
      tttgactac accctaacg gtttattca gactcgaag 
      acgttttat ttggagaaa atggggaaa aaaattgtg 
      attcgtgat caaaatata atcacgttt ttgcaaaat 
      gggattaag gatggctct cttaaaaat aaaagtact 
      gaagattcg tactcgttg aagagcttg cataagaaa 
      attgaagag ttggaaaaa ctaatcaaa aatttaaat 
      ttttccgaa acaaaatca taa
    mRNA sequence
    • >TC033341 RA
      ataaatgcg tcctacaaa tgctaaatg aagcagaag 
      aggaactgg aactcgagc tccctaaaa aaaagccat 
      ggcgggctt taatttcct tctggattt ttggactgt 
      gtaacaatt acggatacg tggtcatgt taacagctg 
      cagctgata taatattca caagtgggg gaaaaagga 
      gcaaggaaa gggaatgca cttatgtat ccacaggga 
      cgatattac tagccgata taattccgg gaatttttg 
      caaaattgg taacgccct ttgtccctt tttgcgtga 
      gagtacgaa ttgtgattt gtgtgttgt tgtcagctt 
      ctggatttc tttttgtgg gttgtggac gaattttga 
      cattgtcta ttattggag ttgttgcaa cgtcctttt 
      cttcaggac ttggtgaag tgacttttc ttcaatata 
      gttcgtatt acgacagta atgtgctct ccacttgga 
      gttcaggca ctggagggg ctggagtta taggcgccc 
      tttcgtacg cgcttttgc aaaacattg gctttacgt 
      taactttga tgataatgc tcctgattc cgtcaacaa 
      tggctttga cttttttca tgcttttgc cgaaaccgc 
      agccaactg agactgcgg taatccagc aagacgtta 
      atgccgaag agatcaaaa aacccaaag agacactaa 
      ttaagaaaa tcatgcaca ttccctctc tctgcaagt 
      acatgatac cgttcggtt tggtttatt ttctggaat 
      attttataa atcaaggaa ctttcgagt tgattcatt 
      ttaaaaata cgcgtttaa gtcccgatg atcagtaca 
      gatggcttc aagttatct accaagtcg gtgttttta 
      tttcacgat cttcggcca atttatttt atataaaac 
      aaatttgga taatgtcgg tgttacaag ctgttaatg 
      tagcaattt ttacaactg aagtcattt atttttatt 
      tgccgaatt tttggattg ttgttgctt taactctgt 
      gggaaggtc tcttaggtg gggccgctt atgttaata 
      catattatc gaattcgta atgaggtca aagaggaaa 
      tgcatcaat tttcaatgg caattacga gtttttccg 
      atagcattg gaatttctc tagctggaa ttgtggcag 
      tgtatgcac ataatgcca tttgcgacc tcccaatgc 
      caaaacata gtatttgta cacgaaaaa aagaggtcc 
      ttaagacaa aattaaatc atcattatg taacgtatc 
      aacgcaccg aattttaca aatgtggat ttctttatt 
      taaaaaaat gtaggagat ataaaaccg aatgctgcc 
      atgacaaca catacatca cacatttca ctgactatg 
      aaactgaga aatattgct gcgatggtt gcgatgttc 
      gcacttttg tgcaaatgt tacaagcaa atgttgtgt 
      atgtgttta cgacaatta ttattattc ttgtgatag 
      tttttctat ttcttcaaa tcagaaaat gtaggaaga 
      aaaactgtg atgttgtag atgaaagtt tggaattaa 
      caaaaaaaa tgactccta aaaattcgg aaaccgaat 
      tctctaaaa tagaacttg aggaatttg acgaaccac 
      caaatgttt ccaaactag ccgaagatc ttttaatcg 
      cagtccaaa aaaacgcca ttcccgaaa ttaaacacc 
      tagtttcgc aaaatcgga gtatcctat catacgaat 
      acccatttt acgagcacc aaaccatcc tactactgg 
      cctgtaacg actcacaca acatgtgct cagtgacac 
      gcgaagtca tcgaaactt taatcgaac tcggagcga 
      attgtaacg aacccagca gaaacgacc actgggaac 
      ccctccatt acaccgccc ttaacgcca acaaaacca 
      aaatgcaaa caattctcc cacacagcc aaatcaact 
      cgctggtct actgctcca aacaaacca ccaaaaagt 
      acgactttt gccccacca tcaaggact cgtactcaa 
      acaatgccc tgaacgttc tcctgaaat gcggcaacc 
      gccagaaac aattcgtcc aatgctgcc aacttctga 
      ttgaaaacg ggatcaacg taaaccaga ccgatagca 
      acggcgtgt ccccgtctg atttaatct ggaaaatgg 
      acaacagcg aacttaaac aactactca gagataaga 
      caagcccga aaatcgttg ataacacct tcgggaaca 
      taaaactga gcaaaatcc agcgcttcc tctccttgg 
      acttgtcac acgaagacg tggacagag tagacggtg 
      ccgattgtc caacttcca gctgcacaa tcctgcagt 
      tgtgctgcg ccaaaggac tgacttcgt gtgtcgtcc 
      accttttgg aaaaaggcg caaacccga ataaaacca 
      tcccgaaga accccaact tgccggtca tgatcgccg 
      tcaattccg accacaagg aaatcgtcg aaatactcc 
      tccagaaga acgcggatt tgcccaaca atgtgttac 
      tccacttgc agcaattgc accgagatg acgacactc 
      tggtcctgg ctgatagat acttaaaaa taattttga 
      gacacttgg ctcggtttg aagccacaa ctgtccaga 
      aatatttaa gttgtaagg acgaacagg gtcggagtg 
      ctttacact acgccattt cctacgact gtcgtgaga 
      atattttag cgctcctgg cacttggag cctccttgg 
      tggagaaag acgacttcg ggaacaaat tacttgagt 
      caattgagc ccaaaactt tggagactt ttttcgaga 
      ataattgca aagtagcgc aaagtcgca caaatgggg 
      agagtaagt ttacagtga ccattgatt acaagtctc 
      tggttgcgg aaacaagcc ctgatagtg attttctcc 
      acactatga cgaaaattc ccgagttga actacttga 
      ccaatcacc ccgtggtgg cgctttatt tggccatga 
      aatggacca aatgtcagt ggaacttat cagtggcgt 
      tttccctag ttttcctca cttccaccc ttggcattt 
      tcatgcagt tgggcaatt tctcgtttt tcacagtca 
      aggttatca ttttgcaag aaatcacga tcacgtttt 
      tcaaatata tagcgtttt atagttttc ccttggtgg 
      cgttttttt tctgctttt acatgctga acgatgaca 
      aagactatt tgtttttcc caatgcttt acgaaactg 
      tgaccatgt tcactgggg attttgacg ccgattatc 
      caatgcact tcaaacgaa acccaattt tcgggcact 
      tgatttacg tggttttcg tcatcttga ttgggatta 
      ttttgcaca acttgctga ttggtctag cggtcaatg 
      atttgcaag cgatttgtt acgaagcga agtttatcg 
      ataagaggg aacgctcga aatatataa caaatgttg 
      aaaatgttc tctttacca agttgcaaa agtcctatt 
      attttagac cgatgtttg aaaacgtgc tgaattttt 
      gtcgcgtgt tcgataatt ttgactaca ccctaacgg 
      tttattcag actcgaaga cgttttatt tggagaaaa 
      tggggaaaa aaattgtga ttcgtgatc aaaatataa 
      tcacgtttt tgcaaaatg ggattaagg atggctctc 
      ttaaaaata aaagtactg aagattcgt actcgttga 
      agagcttgc ataagaaaa ttgaagagt tggaaaaac 
      taatcaaaa atttaaatt tttccgaaa caaaatcat 
      aacaatttt taccgcaat ttcgacaaa ccctttact 
      ttttttcta aatataata ataaattga tattttgaa 
      ccttaatga tatgttatt atgtcactt ccctaaatc 
      accctccga taagtgaat aaataaata aacgtagct 
      ttagtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY