| General information | |||
| TC015446 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9690005..9694833 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC015446 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08485 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015446 RA
MTLKDREIY TATKGVENE GYEFEIAML LFYCLDLSY NTQIRDFKI AIYDNEFHP FDDIVIDVS TETTVKYAI QLKHVKDKL ILSSHHFGR NGKMVLNKY FKFDFTKSD HVVLFTNGV LNEEMSISG CDIKAKKTL TFLEQCLDT SPSTSQHIY EVSNEETKN KITFFTSQK NRNEIDAAI RTKLEEKFK LHEKNLDSV VQKLKFFLQ QWKKGHYKL YKLDKQDIK MKLAEILLQ DSLVQLCPN FGPHIDPKI LLLQDVIKS RTITTFEKP PSGFDTLFC FDLDKNKLI EDGQELLVG DLDEETKIG VILWRLKIF PLVVKTNEE NKQVIREVI TDLRDENLN YILLGGDSP DFDQTFKLL RNLSDLKNS QFYDEIVRT FKLSIQGRA ETTLEELIA WNPNFSQIV TTRELFLMA RGDLHVGEP QEVLPQPYI PRRLFFPVL KIKTIGQFP SDLFAIIGR EDVLKKALP KNTFFRFDD CVGRTFIGG KLPPKSVIL CRDEKDLRN CLGYKNSNV HCLKALDGE NLQLLFSKE KGEELRQFQ IFPENQLTL LPNKIKLVV ARPGLGKLT MLKFLKNNA PVGHWAVKV DLQEEVGDH GEGFEFQVK NCFKEVNRW VYFYCGIDN LQNGGFAKA CQEIREKSG EFQVWVFSE DHLKEQLEK CFEVPAMEI EEFTEEEQQ EYITKRLEE RQKVAKVLQ SFKMLRNND ILGAPMHLY MLTEIFIND PKMEQVLIL TEIYRYFIN TKYQHYRAK TCDTCESNH NQIIEDGIF YRNEQYKLA AISCLDVPS FKKLKLTCD SNFLTQIKS RGDVIGVIF KVSSDHGII FTQQTFSDY FAAIWLAEH HQNVGPEFL QRDDLRNIL FMFNLEVCK NHPAHISVL YQNLQELEK HQLGDLDPL KRSVLHLSF CHGPKFSKI QREIFDLLV QKAEIDPFQ KDELFNWDL LDYAFNLGN FHALERLLG RFATVEGAV LGRLCMVGD LEPLVYYCV KFGCENLLK LASKTPNFG SHVSWEDSN GESLLYLAA KSGSCANTS LLLDQGARI DCATSSYNK MSALHAAVE NNCEKIVDL LLKRQAFVD VMDRYRNTP LHLAASKGL QNCAKTLLN FGADVNFSD NFGRTPLQL AASSGHHHL VELFLGHGA LIDKSDKLG NTPFSAAFG KKHRKICDL LANLGAKND RKPSVGEVD QYYILFPLH WVVFTGQLA EIKSLLDEG YSIDEVDHF GRTPLHRAV IGKHQKAVE VFLDRGADV TICDNYKIM PLTRAIFNE DAETTKLLV FQHASVDFK DSFGYTPLH LAAMCGNVE IVKLLLTKN PDFDVCDDF GRKPVDCAV EGGHSGVVA LLGGDEGRV GRARQMARF LEVRDTPDL GREELESFG FSFKHLVAM LGRFEDLER FVDEEDFVD VCGNTALDW ACKKGHVEC VKVLVGCRA VAKAIQGDF LDMARKQRE FYKVWTLID NKILVCWSI FVLLGTVEY KLGGGTEEF LYLKE
- >TC015446 RA
atgacgcta aaagaccgc gagatatac actgccacc aaaggcgtg gaaaacgaa ggctacgag ttcgaaatc gcaatgtta ttattctac tgtctagac ttaagctac aacacgcaa atccgcgat ttcaaaatc gcaatttac gacaacgaa ttccaccct ttcgacgac atagtcatc gacgtttcc accgaaacc accgtaaaa tacgccatc caattaaaa cacgtcaag gataaactg atcctcagt tcccaccat tttggaaga aacggcaaa atggtccta aacaaatac ttcaagttt gatttcacc aaaagtgac cacgtggtc ctcttcaca aacggcgtc ttaaacgag gaaatgtca atcagtggg tgcgacatc aaagccaaa aaaacccta actttcctg gaacagtgt ctagacacc tctccctca acctcccaa cacatctac gaagtcagc aacgaagaa accaaaaac aaaatcacc ttcttcacc tcccagaag aacagaaac gagattgac gccgccatt cgcacgaaa ctcgaagaa aaattcaaa ctacacgag aaaaaccta gacagcgtt gtccaaaag ttgaagttt ttcctccaa cagtggaag aaaggccac tacaagctg tacaagttg gacaaacag gacattaaa atgaaactc gccgagatc ctcctccag gactccctg gtccagctg tgcccgaat tttgggccc cacatcgac cccaaaatt ttgctcctc caggacgtc atcaaaagt cgcaccatc acgaccttt gaaaaacca ccgagtggc tttgatacg ttgttctgc ttcgatttg gacaaaaac aagctgatt gaagacggc caggagctg ctagttggc gatcttgac gaagaaacg aagattggt gtgattttg tggaggttg aagattttc ccactggtg gtgaagacc aatgaggag aacaaacaa gtgatccgg gaagtgatc accgacttg cgcgacgaa aacttgaac tatatcctc ctcgggggt gattccccc gattttgac caaaccttc aaacttttg agaaactta tctgacttg aagaacagc cagttttat gacgaaatc gtccgaact ttcaaatta tcaattcaa ggacgtgcc gaaaccacg cttgaagaa ttaatcgct tggaaccca aactttagc caaattgtg acaacccgc gagcttttt ctcatggct aggggcgac ctccacgtg ggggaaccc caggaagtg ttaccccag ccgtacatc ccccggcga ctcttcttc cccgttttg aagatcaag acaatcggg caattcccc agcgatttg ttcgcaatc atcggacgt gaagatgtt ctgaagaag gcgctcccc aagaacacg ttcttccgt tttgacgat tgcgtcggg cgcaccttc atcgggggg aaactcccc cccaagtcg gtgattttg tgtcgggac gagaaggac ttgaggaat tgtctaggt tacaagaac agtaacgtc cactgtctc aaagccctg gacggggaa aacctccaa ttgttgttc agtaaggag aagggggag gagttgcgc caattccag atctttcca gagaatcaa ctgaccctc ctccccaat aaaatcaag ttggttgtg gcgcggccc ggccttggg aaactaaca atgttgaag tttttgaaa aataatgcg ccggtgggg cactgggcc gtgaaggtt gacctccag gaggaggtg ggggaccat ggtgaaggt ttcgagttc caggtgaag aattgcttc aaggaggtg aaccgctgg gtttacttc tactgcggt attgataat ctccagaat ggcggtttt gccaaagct tgccaggag atcagggaa aaaagtggg gagttccaa gtttgggtt ttttccgag gaccacttg aaggaacaa ctcgagaaa tgtttcgag gtcccagcc atggaaatc gaggagttt acggaggag gagcaacag gagtatata accaagcgg ttggaggag cgccaaaag gtggcaaaa gtcttgcaa agtttcaaa atgttgcga aacaacgat attttgggc gcgcccatg cacctctac atgttgact gagattttc atcaacgac cccaagatg gagcaagtg ctgattttg accgaaatc taccgctac ttcatcaac accaaatac cagcactac cgcgccaaa acgtgcgac acttgcgaa agcaaccac aaccaaatc attgaagat ggcattttt taccgaaac gagcagtac aaactagct gccatttcg tgtctagac gtaccctcc ttcaaaaaa ttgaaacta acatgtgat agtaatttt ttgacccaa atcaaaagt cgcggcgac gtgattggt gtaattttc aaggtcagt tccgaccat gggatcatt ttcacccaa caaaccttc tccgactac ttcgccgcc atttggttg gcggaacac caccaaaat gtcggaccc gaatttttg caacgtgac gacctccgg aacatcctc ttcatgttc aacctcgaa gtttgcaaa aatcacccg gcacatatt tccgtcctc taccaaaat ctccaagag ttggagaaa caccaactt ggggacttg gaccctttg aagcgctcc gtcctgcac ttgtcgttc tgtcacggg ccgaagttt tccaaaatc cagagggaa attttcgat ctgttggtc caaaaggcc gaaattgat ccgttccag aaggacgag cttttcaac tgggatttg ctggactat gccttcaac ttggggaat ttccacgct ctggagcgc ctgcttggc aggtttgcc accgttgaa ggggcggtt ttggggaga ctttgcatg gtgggcgat ttggaaccg ctggtttat tactgtgtt aagtttggt tgcgaaaat ttgctcaaa ctagcgtcc aaaaccccg aattttggg tcacacgtg tcctgggag gattcaaac ggcgaaagt ttgctctat ctggcggcg aaatcggga agttgtgcc aacaccagc ctccttctc gaccaaggg gccaggatt gactgtgcc accagcagt tacaacaaa atgagtgcg ttacatgcc gctgttgag aataattgt gaaaaaatc gtggatttg ttgctaaaa cgccaggct tttgtggac gtcatggac cgttatagg aacacccct ttgcacttg gcggcaagt aaagggtta caaaactgt gccaaaacc ctgctcaac ttcggagcc gatgtgaat ttttcggac aacttcggc cgaactccc ctacaactg gcggcaagt tccggccac caccacctg gtggagctg ttccttggc cacggggcc ttgattgat aagagcgat aagttgggt aataccccc tttagtgcg gcttttggc aaaaaacac cgcaaaatt tgcgacttg ttggcgaac ttgggggcg aagaacgat cgtaaaccg tccgttggc gaagtggac caatactac atccttttc cccctccac tgggtggtt ttcacgggc cagttggcc gagatcaag tcgctgctg gacgaaggc tactcgatt gatgaggtg gaccacttt ggccgcact cccctacat cgggccgtg atcgggaag caccagaag gcagtcgag gtgtttctg gaccgtgga gctgacgtt accatttgt gacaattac aagatcatg cctttgaca cgcgcgatt tttaacgag gacgccgaa accaccaaa ttgctagtt ttccaacat gcaagtgtc gattttaag gacagtttt ggttacact cccctacat ttggccgca atgtgcggg aacgtcgaa attgtgaaa ctgctcctc accaaaaac cccgatttt gatgtttgt gatgatttc gggcggaag ccggtggat tgcgccgtg gaggggggg cattctggg gtggtggcg ctgcttgga ggggatgag gggagggtg gggagggcg aggcaaatg gcgaggttt ttggaggtt cgggatacg cctgacttg gggagggag gagttggag agttttggg ttcagtttt aagcatttg gtggcgatg ttggggagg tttgaggat ttggagagg tttgtggat gaggaggat tttgtggat gtgtgtggg aatacggcg ttggattgg gcgtgcaag aagggacat gtggagtgt gtgaaggtg ttggtgggg tgtcgggcc gttgcgaag gccatccaa ggggatttc ttggatatg gcgaggaag caaagggag ttttacaag gtttggact cttattgac aataaaatt ttggtttgt tggtcgatt tttgtttta ttgggcacg gtggagtat aagttagga ggcggaacg gaggagttt ctgtacctg aaggagtag
- >TC015446 RA
accgtttat cccgtgata aacacttac gattaatcc aaatatctt atcaatcgc tattttggt cagaaagta acaagttca cgtacaaat atgtgcaaa aaaacgtaa ataaacgac catgattta aaaaaataa acaatatga cgctaaaag accgcgaga tatacactg ccaccaaag gcgtggaaa acgaaggct acgagttcg aaatcgcaa tgttattat tctactgtc tagacttaa gctacaaca cgcaaatcc gcgatttca aaatcgcaa tttacgaca acgaattcc accctttcg acgacatag tcatcgacg tttccaccg aaaccaccg taaaatacg ccatccaat taaaacacg tcaaggata aactgatcc tcagttccc accattttg gaagaaacg gcaaaatgg tcctaaaca aatacttca agtttgatt tcaccaaaa gtgaccacg tggtcctct tcacaaacg gcgtcttaa acgaggaaa tgtcaatca gtgggtgcg acatcaaag ccaaaaaaa ccctaactt tcctggaac agtgtctag acacctctc cctcaacct cccaacaca tctacgaag tcagcaacg aagaaacca aaaacaaaa tcaccttct tcacctccc agaagaaca gaaacgaga ttgacgccg ccattcgca cgaaactcg aagaaaaat tcaaactac acgagaaaa acctagaca gcgttgtcc aaaagttga agtttttcc tccaacagt ggaagaaag gccactaca agctgtaca agttggaca aacaggaca ttaaaatga aactcgccg agatcctcc tccaggact ccctggtcc agctgtgcc cgaattttg ggccccaca tcgacccca aaattttgc tcctccagg acgtcatca aaagtcgca ccatcacga cctttgaaa aaccaccga gtggctttg atacgttgt tctgcttcg atttggaca aaaacaagc tgattgaag acggccagg agctgctag ttggcgatc ttgacgaag aaacgaaga ttggtgtga ttttgtgga ggttgaaga ttttcccac tggtggtga agaccaatg aggagaaca aacaagtga tccgggaag tgatcaccg acttgcgcg acgaaaact tgaactata tcctcctcg ggggtgatt cccccgatt ttgaccaaa ccttcaaac ttttgagaa acttatctg acttgaaga acagccagt tttatgacg aaatcgtcc gaactttca aattatcaa ttcaaggac gtgccgaaa ccacgcttg aagaattaa tcgcttgga acccaaact ttagccaaa ttgtgacaa cccgcgagc tttttctca tggctaggg gcgacctcc acgtggggg aaccccagg aagtgttac cccagccgt acatccccc ggcgactct tcttccccg ttttgaaga tcaagacaa tcgggcaat tccccagcg atttgttcg caatcatcg gacgtgaag atgttctga agaaggcgc tccccaaga acacgttct tccgttttg acgattgcg tcgggcgca ccttcatcg gggggaaac tccccccca agtcggtga ttttgtgtc gggacgaga aggacttga ggaattgtc taggttaca agaacagta acgtccact gtctcaaag ccctggacg gggaaaacc tccaattgt tgttcagta aggagaagg gggaggagt tgcgccaat tccagatct ttccagaga atcaactga ccctcctcc ccaataaaa tcaagttgg ttgtggcgc ggcccggcc ttgggaaac taacaatgt tgaagtttt tgaaaaata atgcgccgg tggggcact gggccgtga aggttgacc tccaggagg aggtggggg accatggtg aaggtttcg agttccagg tgaagaatt gcttcaagg aggtgaacc gctgggttt acttctact gcggtattg ataatctcc agaatggcg gttttgcca aagcttgcc aggagatca gggaaaaaa gtggggagt tccaagttt gggtttttt ccgaggacc acttgaagg aacaactcg agaaatgtt tcgaggtcc cagccatgg aaatcgagg agtttacgg aggaggagc aacaggagt atataacca agcggttgg aggagcgcc aaaaggtgg caaaagtct tgcaaagtt tcaaaatgt tgcgaaaca acgatattt tgggcgcgc ccatgcacc tctacatgt tgactgaga ttttcatca acgacccca agatggagc aagtgctga ttttgaccg aaatctacc gctacttca tcaacacca aataccagc actaccgcg ccaaaacgt gcgacactt gcgaaagca accacaacc aaatcattg aagatggca ttttttacc gaaacgagc agtacaaac tagctgcca tttcgtgtc tagacgtac cctccttca aaaaattga aactaacat gtgatagta attttttga cccaaatca aaagtcgcg gcgacgtga ttggtgtaa ttttcaagg tcagttccg accatggga tcattttca cccaacaaa ccttctccg actacttcg ccgccattt ggttggcgg aacaccacc aaaatgtcg gacccgaat ttttgcaac gtgacgacc tccggaaca tcctcttca tgttcaacc tcgaagttt gcaaaaatc acccggcac atatttccg tcctctacc aaaatctcc aagagttgg agaaacacc aacttgggg acttggacc ctttgaagc gctccgtcc tgcacttgt cgttctgtc acgggccga agttttcca aaatccaga gggaaattt tcgatctgt tggtccaaa aggccgaaa ttgatccgt tccagaagg acgagcttt tcaactggg atttgctgg actatgcct tcaacttgg ggaatttcc acgctctgg agcgcctgc ttggcaggt ttgccaccg ttgaagggg cggttttgg ggagacttt gcatggtgg gcgatttgg aaccgctgg tttattact gtgttaagt ttggttgcg aaaatttgc tcaaactag cgtccaaaa ccccgaatt ttgggtcac acgtgtcct gggaggatt caaacggcg aaagtttgc tctatctgg cggcgaaat cgggaagtt gtgccaaca ccagcctcc ttctcgacc aaggggcca ggattgact gtgccacca gcagttaca acaaaatga gtgcgttac atgccgctg ttgagaata attgtgaaa aaatcgtgg atttgttgc taaaacgcc aggcttttg tggacgtca tggaccgtt ataggaaca cccctttgc acttggcgg caagtaaag ggttacaaa actgtgcca aaaccctgc tcaacttcg gagccgatg tgaattttt cggacaact tcggccgaa ctcccctac aactggcgg caagttccg gccaccacc acctggtgg agctgttcc ttggccacg gggccttga ttgataaga gcgataagt tgggtaata cccccttta gtgcggctt ttggcaaaa aacaccgca aaatttgcg acttgttgg cgaacttgg gggcgaaga acgatcgta aaccgtccg ttggcgaag tggaccaat actacatcc ttttccccc tccactggg tggttttca cgggccagt tggccgaga tcaagtcgc tgctggacg aaggctact cgattgatg aggtggacc actttggcc gcactcccc tacatcggg ccgtgatcg ggaagcacc agaaggcag tcgaggtgt ttctggacc gtggagctg acgttacca tttgtgaca attacaaga tcatgcctt tgacacgcg cgattttta acgaggacg ccgaaacca ccaaattgc tagttttcc aacatgcaa gtgtcgatt ttaaggaca gttttggtt acactcccc tacatttgg ccgcaatgt gcgggaacg tcgaaattg tgaaactgc tcctcacca aaaaccccg attttgatg tttgtgatg atttcgggc ggaagccgg tggattgcg ccgtggagg gggggcatt ctggggtgg tggcgctgc ttggagggg atgagggga gggtgggga gggcgaggc aaatggcga ggtttttgg aggttcggg atacgcctg acttgggga gggaggagt tggagagtt ttgggttca gttttaagc atttggtgg cgatgttgg ggaggtttg aggatttgg agaggtttg tggatgagg aggattttg tggatgtgt gtgggaata cggcgttgg attgggcgt gcaagaagg gacatgtgg agtgtgtga aggtgttgg tggggtgtc gggccgttg cgaaggcca tccaagggg atttcttgg atatggcga ggaagcaaa gggagtttt acaaggttt ggactctta ttgacaata aaattttgg tttgttggt cgatttttg ttttattgg gcacggtgg agtataagt taggaggcg gaacggagg agtttctgt acctgaagg agtagtacg gtggcgtag aaatggggg cgcttcaag cgttttaaa ttaatttaa atccaacaa atttactaa ttatttggg aaaaaatgc agaaatgtt gagttttgc actcttaag agactggga aaactgacg actaaaatt tttataaga aa
