Status
Please wait ...
Gene information
General information
TC015446 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9690005..9694833
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015446
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08485

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015446 RA
      MTLKDREIY TATKGVENE GYEFEIAML LFYCLDLSY 
      NTQIRDFKI AIYDNEFHP FDDIVIDVS TETTVKYAI 
      QLKHVKDKL ILSSHHFGR NGKMVLNKY FKFDFTKSD 
      HVVLFTNGV LNEEMSISG CDIKAKKTL TFLEQCLDT 
      SPSTSQHIY EVSNEETKN KITFFTSQK NRNEIDAAI 
      RTKLEEKFK LHEKNLDSV VQKLKFFLQ QWKKGHYKL 
      YKLDKQDIK MKLAEILLQ DSLVQLCPN FGPHIDPKI 
      LLLQDVIKS RTITTFEKP PSGFDTLFC FDLDKNKLI 
      EDGQELLVG DLDEETKIG VILWRLKIF PLVVKTNEE 
      NKQVIREVI TDLRDENLN YILLGGDSP DFDQTFKLL 
      RNLSDLKNS QFYDEIVRT FKLSIQGRA ETTLEELIA 
      WNPNFSQIV TTRELFLMA RGDLHVGEP QEVLPQPYI 
      PRRLFFPVL KIKTIGQFP SDLFAIIGR EDVLKKALP 
      KNTFFRFDD CVGRTFIGG KLPPKSVIL CRDEKDLRN 
      CLGYKNSNV HCLKALDGE NLQLLFSKE KGEELRQFQ 
      IFPENQLTL LPNKIKLVV ARPGLGKLT MLKFLKNNA 
      PVGHWAVKV DLQEEVGDH GEGFEFQVK NCFKEVNRW 
      VYFYCGIDN LQNGGFAKA CQEIREKSG EFQVWVFSE 
      DHLKEQLEK CFEVPAMEI EEFTEEEQQ EYITKRLEE 
      RQKVAKVLQ SFKMLRNND ILGAPMHLY MLTEIFIND 
      PKMEQVLIL TEIYRYFIN TKYQHYRAK TCDTCESNH 
      NQIIEDGIF YRNEQYKLA AISCLDVPS FKKLKLTCD 
      SNFLTQIKS RGDVIGVIF KVSSDHGII FTQQTFSDY 
      FAAIWLAEH HQNVGPEFL QRDDLRNIL FMFNLEVCK 
      NHPAHISVL YQNLQELEK HQLGDLDPL KRSVLHLSF 
      CHGPKFSKI QREIFDLLV QKAEIDPFQ KDELFNWDL 
      LDYAFNLGN FHALERLLG RFATVEGAV LGRLCMVGD 
      LEPLVYYCV KFGCENLLK LASKTPNFG SHVSWEDSN 
      GESLLYLAA KSGSCANTS LLLDQGARI DCATSSYNK 
      MSALHAAVE NNCEKIVDL LLKRQAFVD VMDRYRNTP 
      LHLAASKGL QNCAKTLLN FGADVNFSD NFGRTPLQL 
      AASSGHHHL VELFLGHGA LIDKSDKLG NTPFSAAFG 
      KKHRKICDL LANLGAKND RKPSVGEVD QYYILFPLH 
      WVVFTGQLA EIKSLLDEG YSIDEVDHF GRTPLHRAV 
      IGKHQKAVE VFLDRGADV TICDNYKIM PLTRAIFNE 
      DAETTKLLV FQHASVDFK DSFGYTPLH LAAMCGNVE 
      IVKLLLTKN PDFDVCDDF GRKPVDCAV EGGHSGVVA 
      LLGGDEGRV GRARQMARF LEVRDTPDL GREELESFG 
      FSFKHLVAM LGRFEDLER FVDEEDFVD VCGNTALDW 
      ACKKGHVEC VKVLVGCRA VAKAIQGDF LDMARKQRE 
      FYKVWTLID NKILVCWSI FVLLGTVEY KLGGGTEEF 
      LYLKE
    CDS sequence
    • >TC015446 RA
      atgacgcta aaagaccgc gagatatac actgccacc 
      aaaggcgtg gaaaacgaa ggctacgag ttcgaaatc 
      gcaatgtta ttattctac tgtctagac ttaagctac 
      aacacgcaa atccgcgat ttcaaaatc gcaatttac 
      gacaacgaa ttccaccct ttcgacgac atagtcatc 
      gacgtttcc accgaaacc accgtaaaa tacgccatc 
      caattaaaa cacgtcaag gataaactg atcctcagt 
      tcccaccat tttggaaga aacggcaaa atggtccta 
      aacaaatac ttcaagttt gatttcacc aaaagtgac 
      cacgtggtc ctcttcaca aacggcgtc ttaaacgag 
      gaaatgtca atcagtggg tgcgacatc aaagccaaa 
      aaaacccta actttcctg gaacagtgt ctagacacc 
      tctccctca acctcccaa cacatctac gaagtcagc 
      aacgaagaa accaaaaac aaaatcacc ttcttcacc 
      tcccagaag aacagaaac gagattgac gccgccatt 
      cgcacgaaa ctcgaagaa aaattcaaa ctacacgag 
      aaaaaccta gacagcgtt gtccaaaag ttgaagttt 
      ttcctccaa cagtggaag aaaggccac tacaagctg 
      tacaagttg gacaaacag gacattaaa atgaaactc 
      gccgagatc ctcctccag gactccctg gtccagctg 
      tgcccgaat tttgggccc cacatcgac cccaaaatt 
      ttgctcctc caggacgtc atcaaaagt cgcaccatc 
      acgaccttt gaaaaacca ccgagtggc tttgatacg 
      ttgttctgc ttcgatttg gacaaaaac aagctgatt 
      gaagacggc caggagctg ctagttggc gatcttgac 
      gaagaaacg aagattggt gtgattttg tggaggttg 
      aagattttc ccactggtg gtgaagacc aatgaggag 
      aacaaacaa gtgatccgg gaagtgatc accgacttg 
      cgcgacgaa aacttgaac tatatcctc ctcgggggt 
      gattccccc gattttgac caaaccttc aaacttttg 
      agaaactta tctgacttg aagaacagc cagttttat 
      gacgaaatc gtccgaact ttcaaatta tcaattcaa 
      ggacgtgcc gaaaccacg cttgaagaa ttaatcgct 
      tggaaccca aactttagc caaattgtg acaacccgc 
      gagcttttt ctcatggct aggggcgac ctccacgtg 
      ggggaaccc caggaagtg ttaccccag ccgtacatc 
      ccccggcga ctcttcttc cccgttttg aagatcaag 
      acaatcggg caattcccc agcgatttg ttcgcaatc 
      atcggacgt gaagatgtt ctgaagaag gcgctcccc 
      aagaacacg ttcttccgt tttgacgat tgcgtcggg 
      cgcaccttc atcgggggg aaactcccc cccaagtcg 
      gtgattttg tgtcgggac gagaaggac ttgaggaat 
      tgtctaggt tacaagaac agtaacgtc cactgtctc 
      aaagccctg gacggggaa aacctccaa ttgttgttc 
      agtaaggag aagggggag gagttgcgc caattccag 
      atctttcca gagaatcaa ctgaccctc ctccccaat 
      aaaatcaag ttggttgtg gcgcggccc ggccttggg 
      aaactaaca atgttgaag tttttgaaa aataatgcg 
      ccggtgggg cactgggcc gtgaaggtt gacctccag 
      gaggaggtg ggggaccat ggtgaaggt ttcgagttc 
      caggtgaag aattgcttc aaggaggtg aaccgctgg 
      gtttacttc tactgcggt attgataat ctccagaat 
      ggcggtttt gccaaagct tgccaggag atcagggaa 
      aaaagtggg gagttccaa gtttgggtt ttttccgag 
      gaccacttg aaggaacaa ctcgagaaa tgtttcgag 
      gtcccagcc atggaaatc gaggagttt acggaggag 
      gagcaacag gagtatata accaagcgg ttggaggag 
      cgccaaaag gtggcaaaa gtcttgcaa agtttcaaa 
      atgttgcga aacaacgat attttgggc gcgcccatg 
      cacctctac atgttgact gagattttc atcaacgac 
      cccaagatg gagcaagtg ctgattttg accgaaatc 
      taccgctac ttcatcaac accaaatac cagcactac 
      cgcgccaaa acgtgcgac acttgcgaa agcaaccac 
      aaccaaatc attgaagat ggcattttt taccgaaac 
      gagcagtac aaactagct gccatttcg tgtctagac 
      gtaccctcc ttcaaaaaa ttgaaacta acatgtgat 
      agtaatttt ttgacccaa atcaaaagt cgcggcgac 
      gtgattggt gtaattttc aaggtcagt tccgaccat 
      gggatcatt ttcacccaa caaaccttc tccgactac 
      ttcgccgcc atttggttg gcggaacac caccaaaat 
      gtcggaccc gaatttttg caacgtgac gacctccgg 
      aacatcctc ttcatgttc aacctcgaa gtttgcaaa 
      aatcacccg gcacatatt tccgtcctc taccaaaat 
      ctccaagag ttggagaaa caccaactt ggggacttg 
      gaccctttg aagcgctcc gtcctgcac ttgtcgttc 
      tgtcacggg ccgaagttt tccaaaatc cagagggaa 
      attttcgat ctgttggtc caaaaggcc gaaattgat 
      ccgttccag aaggacgag cttttcaac tgggatttg 
      ctggactat gccttcaac ttggggaat ttccacgct 
      ctggagcgc ctgcttggc aggtttgcc accgttgaa 
      ggggcggtt ttggggaga ctttgcatg gtgggcgat 
      ttggaaccg ctggtttat tactgtgtt aagtttggt 
      tgcgaaaat ttgctcaaa ctagcgtcc aaaaccccg 
      aattttggg tcacacgtg tcctgggag gattcaaac 
      ggcgaaagt ttgctctat ctggcggcg aaatcggga 
      agttgtgcc aacaccagc ctccttctc gaccaaggg 
      gccaggatt gactgtgcc accagcagt tacaacaaa 
      atgagtgcg ttacatgcc gctgttgag aataattgt 
      gaaaaaatc gtggatttg ttgctaaaa cgccaggct 
      tttgtggac gtcatggac cgttatagg aacacccct 
      ttgcacttg gcggcaagt aaagggtta caaaactgt 
      gccaaaacc ctgctcaac ttcggagcc gatgtgaat 
      ttttcggac aacttcggc cgaactccc ctacaactg 
      gcggcaagt tccggccac caccacctg gtggagctg 
      ttccttggc cacggggcc ttgattgat aagagcgat 
      aagttgggt aataccccc tttagtgcg gcttttggc 
      aaaaaacac cgcaaaatt tgcgacttg ttggcgaac 
      ttgggggcg aagaacgat cgtaaaccg tccgttggc 
      gaagtggac caatactac atccttttc cccctccac 
      tgggtggtt ttcacgggc cagttggcc gagatcaag 
      tcgctgctg gacgaaggc tactcgatt gatgaggtg 
      gaccacttt ggccgcact cccctacat cgggccgtg 
      atcgggaag caccagaag gcagtcgag gtgtttctg 
      gaccgtgga gctgacgtt accatttgt gacaattac 
      aagatcatg cctttgaca cgcgcgatt tttaacgag 
      gacgccgaa accaccaaa ttgctagtt ttccaacat 
      gcaagtgtc gattttaag gacagtttt ggttacact 
      cccctacat ttggccgca atgtgcggg aacgtcgaa 
      attgtgaaa ctgctcctc accaaaaac cccgatttt 
      gatgtttgt gatgatttc gggcggaag ccggtggat 
      tgcgccgtg gaggggggg cattctggg gtggtggcg 
      ctgcttgga ggggatgag gggagggtg gggagggcg 
      aggcaaatg gcgaggttt ttggaggtt cgggatacg 
      cctgacttg gggagggag gagttggag agttttggg 
      ttcagtttt aagcatttg gtggcgatg ttggggagg 
      tttgaggat ttggagagg tttgtggat gaggaggat 
      tttgtggat gtgtgtggg aatacggcg ttggattgg 
      gcgtgcaag aagggacat gtggagtgt gtgaaggtg 
      ttggtgggg tgtcgggcc gttgcgaag gccatccaa 
      ggggatttc ttggatatg gcgaggaag caaagggag 
      ttttacaag gtttggact cttattgac aataaaatt 
      ttggtttgt tggtcgatt tttgtttta ttgggcacg 
      gtggagtat aagttagga ggcggaacg gaggagttt 
      ctgtacctg aaggagtag
    mRNA sequence
    • >TC015446 RA
      accgtttat cccgtgata aacacttac gattaatcc 
      aaatatctt atcaatcgc tattttggt cagaaagta 
      acaagttca cgtacaaat atgtgcaaa aaaacgtaa 
      ataaacgac catgattta aaaaaataa acaatatga 
      cgctaaaag accgcgaga tatacactg ccaccaaag 
      gcgtggaaa acgaaggct acgagttcg aaatcgcaa 
      tgttattat tctactgtc tagacttaa gctacaaca 
      cgcaaatcc gcgatttca aaatcgcaa tttacgaca 
      acgaattcc accctttcg acgacatag tcatcgacg 
      tttccaccg aaaccaccg taaaatacg ccatccaat 
      taaaacacg tcaaggata aactgatcc tcagttccc 
      accattttg gaagaaacg gcaaaatgg tcctaaaca 
      aatacttca agtttgatt tcaccaaaa gtgaccacg 
      tggtcctct tcacaaacg gcgtcttaa acgaggaaa 
      tgtcaatca gtgggtgcg acatcaaag ccaaaaaaa 
      ccctaactt tcctggaac agtgtctag acacctctc 
      cctcaacct cccaacaca tctacgaag tcagcaacg 
      aagaaacca aaaacaaaa tcaccttct tcacctccc 
      agaagaaca gaaacgaga ttgacgccg ccattcgca 
      cgaaactcg aagaaaaat tcaaactac acgagaaaa 
      acctagaca gcgttgtcc aaaagttga agtttttcc 
      tccaacagt ggaagaaag gccactaca agctgtaca 
      agttggaca aacaggaca ttaaaatga aactcgccg 
      agatcctcc tccaggact ccctggtcc agctgtgcc 
      cgaattttg ggccccaca tcgacccca aaattttgc 
      tcctccagg acgtcatca aaagtcgca ccatcacga 
      cctttgaaa aaccaccga gtggctttg atacgttgt 
      tctgcttcg atttggaca aaaacaagc tgattgaag 
      acggccagg agctgctag ttggcgatc ttgacgaag 
      aaacgaaga ttggtgtga ttttgtgga ggttgaaga 
      ttttcccac tggtggtga agaccaatg aggagaaca 
      aacaagtga tccgggaag tgatcaccg acttgcgcg 
      acgaaaact tgaactata tcctcctcg ggggtgatt 
      cccccgatt ttgaccaaa ccttcaaac ttttgagaa 
      acttatctg acttgaaga acagccagt tttatgacg 
      aaatcgtcc gaactttca aattatcaa ttcaaggac 
      gtgccgaaa ccacgcttg aagaattaa tcgcttgga 
      acccaaact ttagccaaa ttgtgacaa cccgcgagc 
      tttttctca tggctaggg gcgacctcc acgtggggg 
      aaccccagg aagtgttac cccagccgt acatccccc 
      ggcgactct tcttccccg ttttgaaga tcaagacaa 
      tcgggcaat tccccagcg atttgttcg caatcatcg 
      gacgtgaag atgttctga agaaggcgc tccccaaga 
      acacgttct tccgttttg acgattgcg tcgggcgca 
      ccttcatcg gggggaaac tccccccca agtcggtga 
      ttttgtgtc gggacgaga aggacttga ggaattgtc 
      taggttaca agaacagta acgtccact gtctcaaag 
      ccctggacg gggaaaacc tccaattgt tgttcagta 
      aggagaagg gggaggagt tgcgccaat tccagatct 
      ttccagaga atcaactga ccctcctcc ccaataaaa 
      tcaagttgg ttgtggcgc ggcccggcc ttgggaaac 
      taacaatgt tgaagtttt tgaaaaata atgcgccgg 
      tggggcact gggccgtga aggttgacc tccaggagg 
      aggtggggg accatggtg aaggtttcg agttccagg 
      tgaagaatt gcttcaagg aggtgaacc gctgggttt 
      acttctact gcggtattg ataatctcc agaatggcg 
      gttttgcca aagcttgcc aggagatca gggaaaaaa 
      gtggggagt tccaagttt gggtttttt ccgaggacc 
      acttgaagg aacaactcg agaaatgtt tcgaggtcc 
      cagccatgg aaatcgagg agtttacgg aggaggagc 
      aacaggagt atataacca agcggttgg aggagcgcc 
      aaaaggtgg caaaagtct tgcaaagtt tcaaaatgt 
      tgcgaaaca acgatattt tgggcgcgc ccatgcacc 
      tctacatgt tgactgaga ttttcatca acgacccca 
      agatggagc aagtgctga ttttgaccg aaatctacc 
      gctacttca tcaacacca aataccagc actaccgcg 
      ccaaaacgt gcgacactt gcgaaagca accacaacc 
      aaatcattg aagatggca ttttttacc gaaacgagc 
      agtacaaac tagctgcca tttcgtgtc tagacgtac 
      cctccttca aaaaattga aactaacat gtgatagta 
      attttttga cccaaatca aaagtcgcg gcgacgtga 
      ttggtgtaa ttttcaagg tcagttccg accatggga 
      tcattttca cccaacaaa ccttctccg actacttcg 
      ccgccattt ggttggcgg aacaccacc aaaatgtcg 
      gacccgaat ttttgcaac gtgacgacc tccggaaca 
      tcctcttca tgttcaacc tcgaagttt gcaaaaatc 
      acccggcac atatttccg tcctctacc aaaatctcc 
      aagagttgg agaaacacc aacttgggg acttggacc 
      ctttgaagc gctccgtcc tgcacttgt cgttctgtc 
      acgggccga agttttcca aaatccaga gggaaattt 
      tcgatctgt tggtccaaa aggccgaaa ttgatccgt 
      tccagaagg acgagcttt tcaactggg atttgctgg 
      actatgcct tcaacttgg ggaatttcc acgctctgg 
      agcgcctgc ttggcaggt ttgccaccg ttgaagggg 
      cggttttgg ggagacttt gcatggtgg gcgatttgg 
      aaccgctgg tttattact gtgttaagt ttggttgcg 
      aaaatttgc tcaaactag cgtccaaaa ccccgaatt 
      ttgggtcac acgtgtcct gggaggatt caaacggcg 
      aaagtttgc tctatctgg cggcgaaat cgggaagtt 
      gtgccaaca ccagcctcc ttctcgacc aaggggcca 
      ggattgact gtgccacca gcagttaca acaaaatga 
      gtgcgttac atgccgctg ttgagaata attgtgaaa 
      aaatcgtgg atttgttgc taaaacgcc aggcttttg 
      tggacgtca tggaccgtt ataggaaca cccctttgc 
      acttggcgg caagtaaag ggttacaaa actgtgcca 
      aaaccctgc tcaacttcg gagccgatg tgaattttt 
      cggacaact tcggccgaa ctcccctac aactggcgg 
      caagttccg gccaccacc acctggtgg agctgttcc 
      ttggccacg gggccttga ttgataaga gcgataagt 
      tgggtaata cccccttta gtgcggctt ttggcaaaa 
      aacaccgca aaatttgcg acttgttgg cgaacttgg 
      gggcgaaga acgatcgta aaccgtccg ttggcgaag 
      tggaccaat actacatcc ttttccccc tccactggg 
      tggttttca cgggccagt tggccgaga tcaagtcgc 
      tgctggacg aaggctact cgattgatg aggtggacc 
      actttggcc gcactcccc tacatcggg ccgtgatcg 
      ggaagcacc agaaggcag tcgaggtgt ttctggacc 
      gtggagctg acgttacca tttgtgaca attacaaga 
      tcatgcctt tgacacgcg cgattttta acgaggacg 
      ccgaaacca ccaaattgc tagttttcc aacatgcaa 
      gtgtcgatt ttaaggaca gttttggtt acactcccc 
      tacatttgg ccgcaatgt gcgggaacg tcgaaattg 
      tgaaactgc tcctcacca aaaaccccg attttgatg 
      tttgtgatg atttcgggc ggaagccgg tggattgcg 
      ccgtggagg gggggcatt ctggggtgg tggcgctgc 
      ttggagggg atgagggga gggtgggga gggcgaggc 
      aaatggcga ggtttttgg aggttcggg atacgcctg 
      acttgggga gggaggagt tggagagtt ttgggttca 
      gttttaagc atttggtgg cgatgttgg ggaggtttg 
      aggatttgg agaggtttg tggatgagg aggattttg 
      tggatgtgt gtgggaata cggcgttgg attgggcgt 
      gcaagaagg gacatgtgg agtgtgtga aggtgttgg 
      tggggtgtc gggccgttg cgaaggcca tccaagggg 
      atttcttgg atatggcga ggaagcaaa gggagtttt 
      acaaggttt ggactctta ttgacaata aaattttgg 
      tttgttggt cgatttttg ttttattgg gcacggtgg 
      agtataagt taggaggcg gaacggagg agtttctgt 
      acctgaagg agtagtacg gtggcgtag aaatggggg 
      cgcttcaag cgttttaaa ttaatttaa atccaacaa 
      atttactaa ttatttggg aaaaaatgc agaaatgtt 
      gagttttgc actcttaag agactggga aaactgacg 
      actaaaatt tttataaga aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY