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Gene information
General information
TC015441 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1050004..1054338
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015441
    iBeetle screen iB_08483

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015441 RA
      MSFRVVRSS KFRHVYGQA LKREQCYDN IRVSKSSWD 
      ATFCAVNPK FLAIIVESA GGGAFIVLP HNKVGRLAA 
      DHPLVGGHK GPVLDIAWC PHNDNVIAS GSEDCVVKV 
      WHIPDGGLI KTLTEPVVD LIYHQRRVG LVLWHPTAL 
      NVLLTAGSD NQVVIWNVG TGEALVKID CHPDIVYSA 
      CFNWDGSQL LTTCKDKKI RIINPRTGE VESEAVCHE 
      GSKATRAIF LRHGLVFTT GFSKMSERQ YSLRTPDAL 
      NEPIVMVEL DTSNGVMFP LYDPDTNLV YLCGKGDSV 
      IRYFEITAE PPFVHYINT FQTPDPQRG IGMMPKRGC 
      DVTSCEISR FYRLNNSGL CQVITMTVP RKSELFQED 
      LYPDTLGDS PALTAEEWV EGKDAEPIL ISLKDGYKP 
      PESKATITV TKRNILDKG GAKTGKTQN ATQVPDKTL 
      QEFADEIRK LKAMIVKHE NRIRALEAE VALSKANAD 
      EDGSGGDGL NAGHALGDD EV
    CDS sequence
    • >TC015441 RA
      atgtctttc cgtgtagtc cgtagttca aagttccgg 
      cacgtctac ggccaggcc cttaagcgc gagcagtgc 
      tatgacaac ataagggtc agtaagagc tcctgggac 
      gccactttc tgtgccgtg aaccccaaa ttcctggca 
      atcatcgtg gagagcgcg gggggcggc gccttcatc 
      gtcctgccc cacaacaag gttgggcgg ctcgccgcc 
      gaccatccg ctcgtcggg gggcacaag gggcccgtg 
      ctcgacatc gcctggtgc ccccacaac gacaacgtc 
      atcgccagc ggctcggaa gactgcgtg gtcaaagtg 
      tggcacata ccggacggg gggctcatc aagacactc 
      accgagccc gtcgtcgac ctcatctat caccaaaga 
      cgcgtgggg ctcgtgctc tggcacccc actgccctc 
      aatgtgctt ctcacggcc gggagcgac aatcaagtg 
      gttatttgg aatgttggc actggagaa gccctagtc 
      aaaatcgat tgtcatcca gatattgtg tacagtgct 
      tgttttaac tgggatggc tcgcaactt ttgaccact 
      tgtaaagac aagaagatt aggattatt aatccaagg 
      actggggaa gtggaatcc gaggcggtt tgccacgaa 
      ggttcaaaa gcaacccgc gccattttc ctccgccat 
      ggtctcgta ttcacgact ggtttttcg aaaatgtcc 
      gaacgtcaa tacagtctt cgtacccct gacgccctt 
      aacgaacca atcgttatg gtagaattg gacacttca 
      aacggtgtc atgttcccg ttatacgac cccgataca 
      aatttggtc tatttatgc ggcaaaggc gactctgtt 
      attcgatat tttgagata acagcagag ccacctttc 
      gttcattac atcaacacg tttcaaaca cctgatcca 
      caacgaggc atcggtatg atgccgaaa cgtggctgt 
      gacgtcacc tcgtgtgaa atttcgagg ttttatcgg 
      ttgaataat tcgggccta tgtcaggtt attacaatg 
      acagttccg cggaagtcg gaacttttc caagaggat 
      ttatatcct gatactttg ggcgatagc ccggcctta 
      actgcagaa gaatgggtc gaaggtaaa gatgcagaa 
      ccgattttg atctcgttg aaagacggt tacaagccg 
      ccagaatcc aaagccaca atcacagtg acgaagaga 
      aatattctg gataagggc ggggccaag acggggaag 
      acgcaaaac gcgacacaa gttccggac aaaacgttg 
      caagagttc gccgatgag ataaggaag ttgaaagcg 
      atgattgtc aagcacgag aatcgaatc cgggctttg 
      gaggcggag gtggcgttg agcaaagcc aacgctgat 
      gaggacggg tcaggtggt gacggcttg aatgccggt 
      catgcgcta ggagacgac gaggtttga
    mRNA sequence
    • >TC015441 RA
      aaataaggg atttctgtc ttatttcat gaactagat 
      atgtggtgc atctaaaca acccgttgt aactttgaa 
      attgtcgcc ccgttcgat gtaaatagc cccctcgtg 
      cgttagcaa taaggcgaa gcaaagatg tctttccgt 
      gtagtccgt agttcaaag ttccggcac gtctacggc 
      caggccctt aagcgcgag cagtgctat gacaacata 
      agggtcagt aagagctcc tgggacgcc actttctgt 
      gccgtgaac cccaaattc ctggcaatc atcgtggag 
      agcgcgggg ggcggcgcc ttcatcgtc ctgccccac 
      aacaaggtt gggcggctc gccgccgac catccgctc 
      gtcgggggg cacaagggg cccgtgctc gacatcgcc 
      tggtgcccc cacaacgac aacgtcatc gccagcggc 
      tcggaagac tgcgtggtc aaagtgtgg cacataccg 
      gacgggggg ctcatcaag acactcacc gagcccgtc 
      gtcgacctc atctatcac caaagacgc gtggggctc 
      gtgctctgg caccccact gccctcaat gtgcttctc 
      acggccggg agcgacaat caagtggtt atttggaat 
      gttggcact ggagaagcc ctagtcaaa atcgattgt 
      catccagat attgtgtac agtgcttgt tttaactgg 
      gatggctcg caacttttg accacttgt aaagacaag 
      aagattagg attattaat ccaaggact ggggaagtg 
      gaatccgag gcggtttgc cacgaaggt tcaaaagca 
      acccgcgcc attttcctc cgccatggt ctcgtattc 
      acgactggt ttttcgaaa atgtccgaa cgtcaatac 
      agtcttcgt acccctgac gcccttaac gaaccaatc 
      gttatggta gaattggac acttcaaac ggtgtcatg 
      ttcccgtta tacgacccc gatacaaat ttggtctat 
      ttatgcggc aaaggcgac tctgttatt cgatatttt 
      gagataaca gcagagcca cctttcgtt cattacatc 
      aacacgttt caaacacct gatccacaa cgaggcatc 
      ggtatgatg ccgaaacgt ggctgtgac gtcacctcg 
      tgtgaaatt tcgaggttt tatcggttg aataattcg 
      ggcctatgt caggttatt acaatgaca gttccgcgg 
      aagtcggaa cttttccaa gaggattta tatcctgat 
      actttgggc gatagcccg gccttaact gcagaagaa 
      tgggtcgaa ggtaaagat gcagaaccg attttgatc 
      tcgttgaaa gacggttac aagccgcca gaatccaaa 
      gccacaatc acagtgacg aagagaaat attctggat 
      aagggcggg gccaagacg gggaagacg caaaacgcg 
      acacaagtt ccggacaaa acgttgcaa gagttcgcc 
      gatgagata aggaagttg aaagcgatg attgtcaag 
      cacgagaat cgaatccgg gctttggag gcggaggtg 
      gcgttgagc aaagccaac gctgatgag gacgggtca 
      ggtggtgac ggcttgaat gccggtcat gcgctagga 
      gacgacgag gtttgaaca aaacaagca caaaagttg 
      ccttaattt atcaaagtg ctcaaattg tttgcaatc 
      gcttctttt tgatacatt acctaagta taatatatt 
      tattttatc atttttttt aacaattat gtcattttt 
      ttatcaccc caaagtgct ctttaatat tatatacga 
      ttgttaatt gcatagata tagatacta ttttcatga 
      aacgttata tttatatag tttataatt tttaggggg 
      gttgtcaat gcggccttt tgattcatc acatgacaa 
      taaatcggg tttttttac aaatatgta tataaagtt 
      ttgtgcaaa tacgacgtg taaatatac tgacttgac 
      tgaaaggct gcatttgac aatccgcca ttgtgaagt 
      ttgtgtgtt ccatgttta acaacgaat aatgtaaat 
      aaatgtata aatatgtaa tgcatttgc agatgaaaa 
      ctaataatt gttttatta gtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY