Status
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Gene information
General information
TC015441 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1050004..1054338
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015441
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08483

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015441 RA
      MSFRVVRSS KFRHVYGQA LKREQCYDN IRVSKSSWD 
ATFCAVNPK FLAIIVESA GGGAFIVLP HNKVGRLAA 
DHPLVGGHK GPVLDIAWC PHNDNVIAS GSEDCVVKV 
WHIPDGGLI KTLTEPVVD LIYHQRRVG LVLWHPTAL 
NVLLTAGSD NQVVIWNVG TGEALVKID CHPDIVYSA 
CFNWDGSQL LTTCKDKKI RIINPRTGE VESEAVCHE 
GSKATRAIF LRHGLVFTT GFSKMSERQ YSLRTPDAL 
NEPIVMVEL DTSNGVMFP LYDPDTNLV YLCGKGDSV 
IRYFEITAE PPFVHYINT FQTPDPQRG IGMMPKRGC 
DVTSCEISR FYRLNNSGL CQVITMTVP RKSELFQED 
LYPDTLGDS PALTAEEWV EGKDAEPIL ISLKDGYKP 
PESKATITV TKRNILDKG GAKTGKTQN ATQVPDKTL 
QEFADEIRK LKAMIVKHE NRIRALEAE VALSKANAD 
EDGSGGDGL NAGHALGDD EV
    CDS sequence
    • >TC015441 RA
      atgtctttc cgtgtagtc cgtagttca aagttccgg 
cacgtctac ggccaggcc cttaagcgc gagcagtgc 
tatgacaac ataagggtc agtaagagc tcctgggac 
gccactttc tgtgccgtg aaccccaaa ttcctggca 
atcatcgtg gagagcgcg gggggcggc gccttcatc 
gtcctgccc cacaacaag gttgggcgg ctcgccgcc 
gaccatccg ctcgtcggg gggcacaag gggcccgtg 
ctcgacatc gcctggtgc ccccacaac gacaacgtc 
atcgccagc ggctcggaa gactgcgtg gtcaaagtg 
tggcacata ccggacggg gggctcatc aagacactc 
accgagccc gtcgtcgac ctcatctat caccaaaga 
cgcgtgggg ctcgtgctc tggcacccc actgccctc 
aatgtgctt ctcacggcc gggagcgac aatcaagtg 
gttatttgg aatgttggc actggagaa gccctagtc 
aaaatcgat tgtcatcca gatattgtg tacagtgct 
tgttttaac tgggatggc tcgcaactt ttgaccact 
tgtaaagac aagaagatt aggattatt aatccaagg 
actggggaa gtggaatcc gaggcggtt tgccacgaa 
ggttcaaaa gcaacccgc gccattttc ctccgccat 
ggtctcgta ttcacgact ggtttttcg aaaatgtcc 
gaacgtcaa tacagtctt cgtacccct gacgccctt 
aacgaacca atcgttatg gtagaattg gacacttca 
aacggtgtc atgttcccg ttatacgac cccgataca 
aatttggtc tatttatgc ggcaaaggc gactctgtt 
attcgatat tttgagata acagcagag ccacctttc 
gttcattac atcaacacg tttcaaaca cctgatcca 
caacgaggc atcggtatg atgccgaaa cgtggctgt 
gacgtcacc tcgtgtgaa atttcgagg ttttatcgg 
ttgaataat tcgggccta tgtcaggtt attacaatg 
acagttccg cggaagtcg gaacttttc caagaggat 
ttatatcct gatactttg ggcgatagc ccggcctta 
actgcagaa gaatgggtc gaaggtaaa gatgcagaa 
ccgattttg atctcgttg aaagacggt tacaagccg 
ccagaatcc aaagccaca atcacagtg acgaagaga 
aatattctg gataagggc ggggccaag acggggaag 
acgcaaaac gcgacacaa gttccggac aaaacgttg 
caagagttc gccgatgag ataaggaag ttgaaagcg 
atgattgtc aagcacgag aatcgaatc cgggctttg 
gaggcggag gtggcgttg agcaaagcc aacgctgat 
gaggacggg tcaggtggt gacggcttg aatgccggt 
catgcgcta ggagacgac gaggtttga
    mRNA sequence
    • >TC015441 RA
      aaataaggg atttctgtc ttatttcat gaactagat 
atgtggtgc atctaaaca acccgttgt aactttgaa 
attgtcgcc ccgttcgat gtaaatagc cccctcgtg 
cgttagcaa taaggcgaa gcaaagatg tctttccgt 
gtagtccgt agttcaaag ttccggcac gtctacggc 
caggccctt aagcgcgag cagtgctat gacaacata 
agggtcagt aagagctcc tgggacgcc actttctgt 
gccgtgaac cccaaattc ctggcaatc atcgtggag 
agcgcgggg ggcggcgcc ttcatcgtc ctgccccac 
aacaaggtt gggcggctc gccgccgac catccgctc 
gtcgggggg cacaagggg cccgtgctc gacatcgcc 
tggtgcccc cacaacgac aacgtcatc gccagcggc 
tcggaagac tgcgtggtc aaagtgtgg cacataccg 
gacgggggg ctcatcaag acactcacc gagcccgtc 
gtcgacctc atctatcac caaagacgc gtggggctc 
gtgctctgg caccccact gccctcaat gtgcttctc 
acggccggg agcgacaat caagtggtt atttggaat 
gttggcact ggagaagcc ctagtcaaa atcgattgt 
catccagat attgtgtac agtgcttgt tttaactgg 
gatggctcg caacttttg accacttgt aaagacaag 
aagattagg attattaat ccaaggact ggggaagtg 
gaatccgag gcggtttgc cacgaaggt tcaaaagca 
acccgcgcc attttcctc cgccatggt ctcgtattc 
acgactggt ttttcgaaa atgtccgaa cgtcaatac 
agtcttcgt acccctgac gcccttaac gaaccaatc 
gttatggta gaattggac acttcaaac ggtgtcatg 
ttcccgtta tacgacccc gatacaaat ttggtctat 
ttatgcggc aaaggcgac tctgttatt cgatatttt 
gagataaca gcagagcca cctttcgtt cattacatc 
aacacgttt caaacacct gatccacaa cgaggcatc 
ggtatgatg ccgaaacgt ggctgtgac gtcacctcg 
tgtgaaatt tcgaggttt tatcggttg aataattcg 
ggcctatgt caggttatt acaatgaca gttccgcgg 
aagtcggaa cttttccaa gaggattta tatcctgat 
actttgggc gatagcccg gccttaact gcagaagaa 
tgggtcgaa ggtaaagat gcagaaccg attttgatc 
tcgttgaaa gacggttac aagccgcca gaatccaaa 
gccacaatc acagtgacg aagagaaat attctggat 
aagggcggg gccaagacg gggaagacg caaaacgcg 
acacaagtt ccggacaaa acgttgcaa gagttcgcc 
gatgagata aggaagttg aaagcgatg attgtcaag 
cacgagaat cgaatccgg gctttggag gcggaggtg 
gcgttgagc aaagccaac gctgatgag gacgggtca 
ggtggtgac ggcttgaat gccggtcat gcgctagga 
gacgacgag gtttgaaca aaacaagca caaaagttg 
ccttaattt atcaaagtg ctcaaattg tttgcaatc 
gcttctttt tgatacatt acctaagta taatatatt 
tattttatc atttttttt aacaattat gtcattttt 
ttatcaccc caaagtgct ctttaatat tatatacga 
ttgttaatt gcatagata tagatacta ttttcatga 
aacgttata tttatatag tttataatt tttaggggg 
gttgtcaat gcggccttt tgattcatc acatgacaa 
taaatcggg tttttttac aaatatgta tataaagtt 
ttgtgcaaa tacgacgtg taaatatac tgacttgac 
tgaaaggct gcatttgac aatccgcca ttgtgaagt 
ttgtgtgtt ccatgttta acaacgaat aatgtaaat 
aaatgtata aatatgtaa tgcatttgc agatgaaaa 
ctaataatt gttttatta gtat
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