General information | |||
TC015441 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 1050004..1054338 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015441 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08483 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015441 RA
MSFRVVRSS KFRHVYGQA LKREQCYDN IRVSKSSWD ATFCAVNPK FLAIIVESA GGGAFIVLP HNKVGRLAA DHPLVGGHK GPVLDIAWC PHNDNVIAS GSEDCVVKV WHIPDGGLI KTLTEPVVD LIYHQRRVG LVLWHPTAL NVLLTAGSD NQVVIWNVG TGEALVKID CHPDIVYSA CFNWDGSQL LTTCKDKKI RIINPRTGE VESEAVCHE GSKATRAIF LRHGLVFTT GFSKMSERQ YSLRTPDAL NEPIVMVEL DTSNGVMFP LYDPDTNLV YLCGKGDSV IRYFEITAE PPFVHYINT FQTPDPQRG IGMMPKRGC DVTSCEISR FYRLNNSGL CQVITMTVP RKSELFQED LYPDTLGDS PALTAEEWV EGKDAEPIL ISLKDGYKP PESKATITV TKRNILDKG GAKTGKTQN ATQVPDKTL QEFADEIRK LKAMIVKHE NRIRALEAE VALSKANAD EDGSGGDGL NAGHALGDD EV
- >TC015441 RA
atgtctttc cgtgtagtc cgtagttca aagttccgg cacgtctac ggccaggcc cttaagcgc gagcagtgc tatgacaac ataagggtc agtaagagc tcctgggac gccactttc tgtgccgtg aaccccaaa ttcctggca atcatcgtg gagagcgcg gggggcggc gccttcatc gtcctgccc cacaacaag gttgggcgg ctcgccgcc gaccatccg ctcgtcggg gggcacaag gggcccgtg ctcgacatc gcctggtgc ccccacaac gacaacgtc atcgccagc ggctcggaa gactgcgtg gtcaaagtg tggcacata ccggacggg gggctcatc aagacactc accgagccc gtcgtcgac ctcatctat caccaaaga cgcgtgggg ctcgtgctc tggcacccc actgccctc aatgtgctt ctcacggcc gggagcgac aatcaagtg gttatttgg aatgttggc actggagaa gccctagtc aaaatcgat tgtcatcca gatattgtg tacagtgct tgttttaac tgggatggc tcgcaactt ttgaccact tgtaaagac aagaagatt aggattatt aatccaagg actggggaa gtggaatcc gaggcggtt tgccacgaa ggttcaaaa gcaacccgc gccattttc ctccgccat ggtctcgta ttcacgact ggtttttcg aaaatgtcc gaacgtcaa tacagtctt cgtacccct gacgccctt aacgaacca atcgttatg gtagaattg gacacttca aacggtgtc atgttcccg ttatacgac cccgataca aatttggtc tatttatgc ggcaaaggc gactctgtt attcgatat tttgagata acagcagag ccacctttc gttcattac atcaacacg tttcaaaca cctgatcca caacgaggc atcggtatg atgccgaaa cgtggctgt gacgtcacc tcgtgtgaa atttcgagg ttttatcgg ttgaataat tcgggccta tgtcaggtt attacaatg acagttccg cggaagtcg gaacttttc caagaggat ttatatcct gatactttg ggcgatagc ccggcctta actgcagaa gaatgggtc gaaggtaaa gatgcagaa ccgattttg atctcgttg aaagacggt tacaagccg ccagaatcc aaagccaca atcacagtg acgaagaga aatattctg gataagggc ggggccaag acggggaag acgcaaaac gcgacacaa gttccggac aaaacgttg caagagttc gccgatgag ataaggaag ttgaaagcg atgattgtc aagcacgag aatcgaatc cgggctttg gaggcggag gtggcgttg agcaaagcc aacgctgat gaggacggg tcaggtggt gacggcttg aatgccggt catgcgcta ggagacgac gaggtttga
- >TC015441 RA
aaataaggg atttctgtc ttatttcat gaactagat atgtggtgc atctaaaca acccgttgt aactttgaa attgtcgcc ccgttcgat gtaaatagc cccctcgtg cgttagcaa taaggcgaa gcaaagatg tctttccgt gtagtccgt agttcaaag ttccggcac gtctacggc caggccctt aagcgcgag cagtgctat gacaacata agggtcagt aagagctcc tgggacgcc actttctgt gccgtgaac cccaaattc ctggcaatc atcgtggag agcgcgggg ggcggcgcc ttcatcgtc ctgccccac aacaaggtt gggcggctc gccgccgac catccgctc gtcgggggg cacaagggg cccgtgctc gacatcgcc tggtgcccc cacaacgac aacgtcatc gccagcggc tcggaagac tgcgtggtc aaagtgtgg cacataccg gacgggggg ctcatcaag acactcacc gagcccgtc gtcgacctc atctatcac caaagacgc gtggggctc gtgctctgg caccccact gccctcaat gtgcttctc acggccggg agcgacaat caagtggtt atttggaat gttggcact ggagaagcc ctagtcaaa atcgattgt catccagat attgtgtac agtgcttgt tttaactgg gatggctcg caacttttg accacttgt aaagacaag aagattagg attattaat ccaaggact ggggaagtg gaatccgag gcggtttgc cacgaaggt tcaaaagca acccgcgcc attttcctc cgccatggt ctcgtattc acgactggt ttttcgaaa atgtccgaa cgtcaatac agtcttcgt acccctgac gcccttaac gaaccaatc gttatggta gaattggac acttcaaac ggtgtcatg ttcccgtta tacgacccc gatacaaat ttggtctat ttatgcggc aaaggcgac tctgttatt cgatatttt gagataaca gcagagcca cctttcgtt cattacatc aacacgttt caaacacct gatccacaa cgaggcatc ggtatgatg ccgaaacgt ggctgtgac gtcacctcg tgtgaaatt tcgaggttt tatcggttg aataattcg ggcctatgt caggttatt acaatgaca gttccgcgg aagtcggaa cttttccaa gaggattta tatcctgat actttgggc gatagcccg gccttaact gcagaagaa tgggtcgaa ggtaaagat gcagaaccg attttgatc tcgttgaaa gacggttac aagccgcca gaatccaaa gccacaatc acagtgacg aagagaaat attctggat aagggcggg gccaagacg gggaagacg caaaacgcg acacaagtt ccggacaaa acgttgcaa gagttcgcc gatgagata aggaagttg aaagcgatg attgtcaag cacgagaat cgaatccgg gctttggag gcggaggtg gcgttgagc aaagccaac gctgatgag gacgggtca ggtggtgac ggcttgaat gccggtcat gcgctagga gacgacgag gtttgaaca aaacaagca caaaagttg ccttaattt atcaaagtg ctcaaattg tttgcaatc gcttctttt tgatacatt acctaagta taatatatt tattttatc atttttttt aacaattat gtcattttt ttatcaccc caaagtgct ctttaatat tatatacga ttgttaatt gcatagata tagatacta ttttcatga aacgttata tttatatag tttataatt tttaggggg gttgtcaat gcggccttt tgattcatc acatgacaa taaatcggg tttttttac aaatatgta tataaagtt ttgtgcaaa tacgacgtg taaatatac tgacttgac tgaaaggct gcatttgac aatccgcca ttgtgaagt ttgtgtgtt ccatgttta acaacgaat aatgtaaat aaatgtata aatatgtaa tgcatttgc agatgaaaa ctaataatt gttttatta gtat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
