Status
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Gene information
General information
TC033332 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 884812..893291
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033332
    iBeetle screen iB_08482

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033332 RA
      MVKEFGGQS KTTRNGITS KNNGTVSNN KTKTHYLNF 
      GEEDQMEIS GYTSNHLKK CITWTVYVL TFGILRLIF 
      HWYPQWQLY ASHSKCPLG RAEKVLITD KYKNKFKTY 
      FVKDVEILT SSNELRKTE SIISINGLI FKNETEGHL 
      LDVYLDDGT RKRLTQVRI VRCKKLTYI WDEEKYKFI 
      KLSGLDKGI ACREFHEQK GYKKEEQNR RRITYGTNE 
      INVPVQSIV TLLVLEALT PFYIFQLFS LIVWFAEAY 
      YYYTIAIVI MSVFGISTS IIQTRKSQE NLKGTVHTA 
      DKIMVSRSD GTFDEIPTT ELVPGDVIV IPSHGCDMQ 
      CDAVLLNGN CIVNESMLT GESVPVTKT ALPNNDKLY 
      NVKEHGNHT LFCGTKIIQ TRYYGTEPV LAVVIRTGY 
      LTTKGQLVR SIIYPPPAD FKFDQDSYK FIMILSVIA 
      LLGFFYTIF SKYSRDIAP LDILIKALD LVTIAIPPA 
      LPAAMTVGK LYALNRLKK KHIYCINSR VINVSGSVD 
      CVCFDKTGT LTEDGLDMW GVVPVEDSR IEKPVKEIK 
      TMSKNSLLF RGMLTCHSL TLIDGELCG DPLDIKMFE 
      STGWTLEEP TISDTSKYD LLVPTILKD PSPDTPHHE 
      IGLIHQFQF SSTLQRMSV ICRTLGSDH FESFTKGSP 
      EMVISLSRP ETVPEGILN RLKTYTEQG YRVIGMATK 
      KLTNIPFHK IPKLQREEI ECDLQFVGL IVLENRLKP 
      QTGSVIQKL RNAGMKIVM VTGDNIQTA VSVARDCGI 
      IQSGYSVID VITTKPTKT DMATVKYQE TDATPSGDK 
      MSDIEKMAE RRYHFVVTG NTWTDLNRY FPELIPKIV 
      TKGVVFARM SGLQKQQLV EELQNLGYY VAMCGDGAN 
      DCGALKAAN VGISLSEAE SSVASPFTS QEPNISCTV 
      EVIKEGRAA LVTSFGVFK LMLCYSLTE FASVIILYA 
      IDTNLTSLQ FLFIDICLI LNFASFFGK TRAYDELHR 
      GPPMTSLLS FVPLASIIL FMLVTVAVQ IFAYYYIQT 
      YDWFVPFVF DPNDTTMYR CYENYAVYC VSMFQYIIM 
      AVVFSKGKP YRKAIYTNF IFLFAIFLM IGVCSYITL 
      DPVAWVIEA LELQMPPFY DGRVAILVM ALVNFLICV 
      VIEDVFVDV LLFRFVRPK FRILEKSRQ IYLKIEKEL 
      REDPTWPTK CHSVSYGEI KEGGIVNNG FCNHTETTK 
      C
    CDS sequence
    • >TC033332 RA
      atggtgaag gaatttggt gggcaaagt aagacaaca 
      cgaaatgga ataacgtca aaaaacaat ggcaccgta 
      tcaaacaat aaaactaaa acacactac ctcaatttc 
      ggggaagaa gaccaaatg gaaatttct ggttacacg 
      tccaaccac ttgaagaaa tgtatcacg tggacggtt 
      tatgtcctc acttttgga attttgaga cttattttt 
      cattggtat ccacaatgg cagctgtac gccagccat 
      tccaagtgt cctttaggt cgagccgag aaagtacta 
      atcacagac aaatacaag aacaagttc aagacgtat 
      ttcgtgaaa gacgttgaa attttgaca agcagtaac 
      gaattacgc aaaaccgaa agtattatt agcataaat 
      ggccttatt tttaaaaac gaaacagaa ggacatctc 
      ctcgatgtg tacctggat gatggtacc agaaaacga 
      ttgacacaa gtacgaatc gtacggtgc aaaaagttg 
      acctacatc tgggacgag gaaaagtac aaatttatc 
      aaactgtca ggtttggat aaaggtata gcatgtagg 
      gaatttcac gagcaaaaa gggtacaaa aaagaggaa 
      caaaatcga agacgtatc acttatggt accaacgaa 
      atcaacgta cctgtgcaa agcattgtc acacttttg 
      gtccttgaa gctttgact cctttctac attttccaa 
      ctttttagt ctcatagtt tggtttgcc gaggcctac 
      tattactac actattgca atcgtgata atgtcggtt 
      tttggcatt tccacctcg atcatacaa accagaaag 
      agtcaggaa aatctgaaa ggaaccgtg cacaccgct 
      gacaagatc atggtaagt cgaagtgat ggtactttc 
      gatgaaata ccaacaacc gagctggta ccgggtgac 
      gtaatcgtg ataccatcg catggttgc gacatgcaa 
      tgtgatgcc gttctactc aatggtaac tgcatagta 
      aatgaaagt atgctcacg ggagagtcg gtaccagtc 
      actaaaacc gcacttcca aataacgat aagttatac 
      aatgttaag gaacacgga aaccataca cttttttgc 
      ggtaccaaa atcatacag acgaggtac tatggtacc 
      gaaccggta cttgcagta gttatcagg accgggtat 
      ttgaccaca aaggggcaa ctagtgagg agtataata 
      tacccacca ccggctgat ttcaagttc gatcaagac 
      agctataaa tttatcatg atattgtcg gttatagca 
      ctgttgggg tttttttac acgattttt tcaaagtac 
      tcccgagac attgcacct ctggatatc ctcatcaaa 
      gcgctcgat ttggtcaca atagccata cctcccgcc 
      ctgccagct gcgatgaca gtgggcaaa ctttacgct 
      ttaaatcgt ttgaaaaag aagcacatt tattgcata 
      aattcgaga gtaattaac gtttccggt tccgtcgat 
      tgtgtttgt tttgataag acagggacc ttgactgaa 
      gatggatta gatatgtgg ggggtggtt ccggttgaa 
      gacagtaga attgaaaaa ccagtcaag gagattaag 
      acaatgtcg aaaaattcg ctacttttc agggggatg 
      ctgacttgt cattcgctg acgctgatc gatggggaa 
      ctatgtggg gatccccta gatattaag atgttcgag 
      tcaactggc tggaccctg gaggagcct acaatctcc 
      gacacctcc aaatacgac ctcctggtg cccaccatc 
      ctcaaagac ccctccccc gacaccccc caccacgaa 
      atcggcctc atccaccaa ttccaattc tcctcaacc 
      ctccaacgc atgagcgtg atttgtcga accctaggt 
      tccgaccac ttcgaatcc ttcaccaag ggatccccc 
      gaaatggtc atctcccta tctcgtcca gaaaccgtc 
      cccgaggga atcctcaac cggttaaaa acctacacc 
      gagcagggc taccgcgtc atcggaatg gccaccaaa 
      aaactaacc aacatcccc ttccacaaa atccccaaa 
      ctccaacgg gaggaaatc gaatgcgac ctccaattc 
      gtcggttta atagtccta gaaaatcgg ctaaaaccc 
      caaactggg agcgtcatc caaaagcta cgaaacgcc 
      ggaatgaag atagttatg gtgacgggg gataacatc 
      cagacggct gtgagcgtg gcacgtgac tgcgggatc 
      atccaatcg gggtacagc gtcattgac gtcatcacg 
      accaagccg actaagacg gacatggca actgtcaag 
      taccaggaa actgatgcc acgccttcc ggcgacaaa 
      atgtccgac atcgagaaa atggccgaa cggaggtac 
      cattttgtc gtgaccgga aacacgtgg accgacctt 
      aaccggtac ttccccgaa ctgatcccg aaaattgtg 
      acaaaaggc gtcgttttt gcccgaatg tcgggacta 
      cagaagcaa caactggtg gaagaatta caaaatttg 
      ggttattac gttgccatg tgtggggac ggtgccaat 
      gattgtggc gccctcaag gcggcaaac gtcggaatt 
      tcactttcg gaggctgaa tcaagcgtt gcgtcacct 
      tttacgtca caagaaccc aacatttcc tgtactgtt 
      gaagtgatc aaagaaggc agggccgct ctggtgact 
      tcgtttggg gtttttaag ctcatgctt tgctacagt 
      ctgaccgag ttcgcgtct gttattatc ttgtacgcc 
      attgatacc aacctaact tcgctacaa ttcctcttc 
      attgatatt tgcctaatt ttgaatttc gcctcgttt 
      tttggtaaa acacgagct tatgacgag ttacacaga 
      gggcccccg atgacgtca ctcttgagt ttcgtcccg 
      ttggcatca atcatactt tttatgttg gtcacagtg 
      gccgtgcaa atcttcgcc tactattac atccagact 
      tatgactgg ttcgttccg tttgttttc gacccgaat 
      gacacaaca atgtacagg tgttacgaa aattatgcc 
      gtttattgc gtctccatg tttcagtac atcataatg 
      gcggtcgtt ttttccaaa ggaaaaccg taccgtaaa 
      gcgatctat accaacttt attttcctg tttgctatt 
      tttttaatg attggcgtt tgttcctac attactttg 
      gaccccgtt gcttgggtt attgaagct ttggagttg 
      caaatgccg ccattttat gacggacgg gtcgccatt 
      ttggtcatg gcgcttgtc aattttctc atttgtgtc 
      gtcatcgaa gacgtattc gttgatgtt cttttgttt 
      cgatttgtg aggcctaaa tttcgcatt ttggaaaag 
      tcgaggcaa atttatttg aaaatcgaa aaggaacta 
      agggaggat ccaacatgg ccgaccaaa tgtcacagt 
      gttagttac ggggagatc aaggaaggc gggattgtc 
      aataacggg ttttgcaac cacacagag acgaccaag 
      tgttga
    mRNA sequence
    • >TC033332 RA
      aagaccgac tccacatgt tctaagtga aagtgtttg 
      gtttattat ttcatacaa tacattgcc gttattgcg 
      gtatggtga aggaatttg gtgggcaaa gtaagacaa 
      cacgaaatg gaataacgt caaaaaaca atggcaccg 
      tatcaaaca ataaaacta aaacacact acctcaatt 
      tcggggaag aagaccaaa tggaaattt ctggttaca 
      cgtccaacc acttgaaga aatgtatca cgtggacgg 
      tttatgtcc tcacttttg gaattttga gacttattt 
      ttcattggt atccacaat ggcagctgt acgccagcc 
      attccaagt gtcctttag gtcgagccg agaaagtac 
      taatcacag acaaataca agaacaagt tcaagacgt 
      atttcgtga aagacgttg aaattttga caagcagta 
      acgaattac gcaaaaccg aaagtatta ttagcataa 
      atggcctta tttttaaaa acgaaacag aaggacatc 
      tcctcgatg tgtacctgg atgatggta ccagaaaac 
      gattgacac aagtacgaa tcgtacggt gcaaaaagt 
      tgacctaca tctgggacg aggaaaagt acaaattta 
      tcaaactgt caggtttgg ataaaggta tagcatgta 
      gggaatttc acgagcaaa aagggtaca aaaaagagg 
      aacaaaatc gaagacgta tcacttatg gtaccaacg 
      aaatcaacg tacctgtgc aaagcattg tcacacttt 
      tggtccttg aagctttga ctcctttct acattttcc 
      aacttttta gtctcatag tttggtttg ccgaggcct 
      actattact acactattg caatcgtga taatgtcgg 
      tttttggca tttccacct cgatcatac aaaccagaa 
      agagtcagg aaaatctga aaggaaccg tgcacaccg 
      ctgacaaga tcatggtaa gtcgaagtg atggtactt 
      tcgatgaaa taccaacaa ccgagctgg taccgggtg 
      acgtaatcg tgataccat cgcatggtt gcgacatgc 
      aatgtgatg ccgttctac tcaatggta actgcatag 
      taaatgaaa gtatgctca cgggagagt cggtaccag 
      tcactaaaa ccgcacttc caaataacg ataagttat 
      acaatgtta aggaacacg gaaaccata cactttttt 
      gcggtacca aaatcatac agacgaggt actatggta 
      ccgaaccgg tacttgcag tagttatca ggaccgggt 
      atttgacca caaaggggc aactagtga ggagtataa 
      tatacccac caccggctg atttcaagt tcgatcaag 
      acagctata aatttatca tgatattgt cggttatag 
      cactgttgg ggttttttt acacgattt tttcaaagt 
      actcccgag acattgcac ctctggata tcctcatca 
      aagcgctcg atttggtca caatagcca tacctcccg 
      ccctgccag ctgcgatga cagtgggca aactttacg 
      ctttaaatc gtttgaaaa agaagcaca tttattgca 
      taaattcga gagtaatta acgtttccg gttccgtcg 
      attgtgttt gttttgata agacaggga ccttgactg 
      aagatggat tagatatgt ggggggtgg ttccggttg 
      aagacagta gaattgaaa aaccagtca aggagatta 
      agacaatgt cgaaaaatt cgctacttt tcaggggga 
      tgctgactt gtcattcgc tgacgctga tcgatgggg 
      aactatgtg gggatcccc tagatatta agatgttcg 
      agtcaactg gctggaccc tggaggagc ctacaatct 
      ccgacacct ccaaatacg acctcctgg tgcccacca 
      tcctcaaag acccctccc ccgacaccc cccaccacg 
      aaatcggcc tcatccacc aattccaat tctcctcaa 
      ccctccaac gcatgagcg tgatttgtc gaaccctag 
      gttccgacc acttcgaat ccttcacca agggatccc 
      ccgaaatgg tcatctccc tatctcgtc cagaaaccg 
      tccccgagg gaatcctca accggttaa aaacctaca 
      ccgagcagg gctaccgcg tcatcggaa tggccacca 
      aaaaactaa ccaacatcc ccttccaca aaatcccca 
      aactccaac gggaggaaa tcgaatgcg acctccaat 
      tcgtcggtt taatagtcc tagaaaatc ggctaaaac 
      cccaaactg ggagcgtca tccaaaagc tacgaaacg 
      ccggaatga agatagtta tggtgacgg gggataaca 
      tccagacgg ctgtgagcg tggcacgtg actgcggga 
      tcatccaat cggggtaca gcgtcattg acgtcatca 
      cgaccaagc cgactaaga cggacatgg caactgtca 
      agtaccagg aaactgatg ccacgcctt ccggcgaca 
      aaatgtccg acatcgaga aaatggccg aacggaggt 
      accattttg tcgtgaccg gaaacacgt ggaccgacc 
      ttaaccggt acttccccg aactgatcc cgaaaattg 
      tgacaaaag gcgtcgttt ttgcccgaa tgtcgggac 
      tacagaagc aacaactgg tggaagaat tacaaaatt 
      tgggttatt acgttgcca tgtgtgggg acggtgcca 
      atgattgtg gcgccctca aggcggcaa acgtcggaa 
      tttcacttt cggaggctg aatcaagcg ttgcgtcac 
      cttttacgt cacaagaac ccaacattt cctgtactg 
      ttgaagtga tcaaagaag gcagggccg ctctggtga 
      cttcgtttg gggttttta agctcatgc tttgctaca 
      gtctgaccg agttcgcgt ctgttatta tcttgtacg 
      ccattgata ccaacctaa cttcgctac aattcctct 
      tcattgata tttgcctaa ttttgaatt tcgcctcgt 
      tttttggta aaacacgag cttatgacg agttacaca 
      gagggcccc cgatgacgt cactcttga gtttcgtcc 
      cgttggcat caatcatac tttttatgt tggtcacag 
      tggccgtgc aaatcttcg cctactatt acatccaga 
      cttatgact ggttcgttc cgtttgttt tcgacccga 
      atgacacaa caatgtaca ggtgttacg aaaattatg 
      ccgtttatt gcgtctcca tgtttcagt acatcataa 
      tggcggtcg ttttttcca aaggaaaac cgtaccgta 
      aagcgatct ataccaact ttattttcc tgtttgcta 
      tttttttaa tgattggcg tttgttcct acattactt 
      tggaccccg ttgcttggg ttattgaag ctttggagt 
      tgcaaatgc cgccatttt atgacggac gggtcgcca 
      ttttggtca tggcgcttg tcaattttc tcatttgtg 
      tcgtcatcg aagacgtat tcgttgatg ttcttttgt 
      ttcgatttg tgaggccta aatttcgca ttttggaaa 
      agtcgaggc aaatttatt tgaaaatcg aaaaggaac 
      taagggagg atccaacat ggccgacca aatgtcaca 
      gtgttagtt acggggaga tcaaggaag gcgggattg 
      tcaataacg ggttttgca accacacag agacgacca 
      agtgttgat tttattgta ttttatcat ttttgaata 
      aatatttta caaacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY