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Gene information
General information
TC033316
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015417
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 579411..582696
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033316
    iBeetle screen iB_08474

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033316 RA
      MFSSLLSRA MCQNGAPSE APQEMGLVK SLVAGRTIA 
      AAAAASKTA EGDSCEFGS AHYFALCGM GGILSCGIT 
      HTAIVPLDL VKCRIQVDP NKYKNVFTG FKVTMKEDG 
      FKGLALGWA PTFFGYSAQ GLCKFGLYE VFKVFYTDL 
      IGDENAFLY RTWLYLAAS ASAEFFADI ALSPMEAAK 
      VKIQTSPGF ATTLRTAWP RIYSEEGIN GFYKSLVPL 
      WMRQIPYTM MKFACFERT VELLYKHVV PKPRSECSK 
      GEQLIVTFA AGYIAGVFC AIVSHPADT VVSKLNQEK 
      GSTALEAAK KLGMAGLWK GLTPRIVMI GTLTALQWF 
      IYDAFKVAM RMPRPPPPE MPESLKKRL QAEGKL
    CDS sequence
    • >TC033316 RA
      atgttctcg tcgttgttg tcgcgcgcg atgtgccag 
      aatggggcc cccagcgag gccccgcaa gaaatgggg 
      ctggtgaag tccctcgtt gctgggagg acgatagcg 
      gcggcggcg gcagcctcc aagaccgcc gaaggagac 
      agttgcgag ttcggctcg gcgcactat ttcgccctg 
      tgcggaatg gggggcatc ctgtcttgt gggatcaca 
      catacggca attgtaccc cttgatctg gtcaagtgc 
      cggattcaa gtcgatccc aacaagtat aagaacgta 
      ttcaccggg tttaaggtg accatgaaa gaggacggg 
      ttcaagggc ttggccttg ggatgggcc cccacgttc 
      tttgggtat tcggcgcaa gggttgtgc aagtttgga 
      ttgtatgag gtttttaag gtattttac accgacttg 
      attggcgac gaaaacgcc ttcttgtac cgcacttgg 
      ctgtatttg gccgccagt gcttcagcg gaatttttc 
      gccgacatt gcgctttcg ccaatggaa gccgccaaa 
      gtgaaaatc caaacttcc cctggcttt gccaccact 
      ttgcgcacc gcctggcct agaatctac tccgaggag 
      ggcattaac ggcttctac aaaagcttg gtgcctctg 
      tggatgcgc cagatcccc tacaccatg atgaagttc 
      gcctgcttc gagcgaact gtcgaactc ctgtacaaa 
      catgtggtc cccaaacca cgctcagag tgcagcaaa 
      ggcgaacag ttgattgtc actttcgct gccggttac 
      atagctggc gttttctgc gcgattgtc agccatcct 
      gcggacact gtggtgtcc aagttgaac caggagaag 
      ggctcgacg gccctggag gccgccaaa aagctgggc 
      atggccgga ctgtggaaa gggctgacg cccaggatt 
      gtaatgatt ggtactttg accgcattg caatggttt 
      atctatgat gcgttcaaa gtggcaatg cgtatgcca 
      cgcccaccg ccaccggaa atgcccgag tcgcttaag 
      aagagatta caagccgag ggcaaattg taa
    mRNA sequence
    • >TC033316 RA
      tgataaatg tgggtgtta acatttttt ttcttattt 
      tttcaattt tcaaaattt tcaaaagca gtgaagaaa 
      taattgatt atttaccat tatgagact aaggcctta 
      agggcaagt gacaaaccc gatgaacga atttcccca 
      caaacaact gcctttgct tcaaaattt tatcacaaa 
      aaaagactc aaatttatc attgggttt gttgcaaaa 
      atagccaaa atcatgacc tgtctccaa tgcccctta 
      cgcaacaat ttaattatg tcaaacacg tgaccttga 
      tcaatgtcc acgtgcact tgcgcaata cagcgccat 
      attaccacc agcggagac cacgagaac taccttcac 
      ggggctgta gccattgtt tccaggccg ctttaacac 
      cgatcacga tgttctcgt cgttgttgt cgcgcgcga 
      tgtgccaga atggggccc ccagcgagg ccccgcaag 
      aaatggggc tggtgaagt ccctcgttg ctgggagga 
      cgatagcgg cggcggcgg cagcctcca agaccgccg 
      aaggagaca gttgcgagt tcggctcgg cgcactatt 
      tcgccctgt gcggaatgg ggggcatcc tgtcttgtg 
      ggatcacac atacggcaa ttgtacccc ttgatctgg 
      tcaagtgcc ggattcaag tcgatccca acaagtata 
      agaacgtat tcaccgggt ttaaggtga ccatgaaag 
      aggacgggt tcaagggct tggccttgg gatgggccc 
      ccacgttct ttgggtatt cggcgcaag ggttgtgca 
      agtttggat tgtatgagg tttttaagg tattttaca 
      ccgacttga ttggcgacg aaaacgcct tcttgtacc 
      gcacttggc tgtatttgg ccgccagtg cttcagcgg 
      aatttttcg ccgacattg cgctttcgc caatggaag 
      ccgccaaag tgaaaatcc aaacttccc ctggctttg 
      ccaccactt tgcgcaccg cctggccta gaatctact 
      ccgaggagg gcattaacg gcttctaca aaagcttgg 
      tgcctctgt ggatgcgcc agatcccct acaccatga 
      tgaagttcg cctgcttcg agcgaactg tcgaactcc 
      tgtacaaac atgtggtcc ccaaaccac gctcagagt 
      gcagcaaag gcgaacagt tgattgtca ctttcgctg 
      ccggttaca tagctggcg ttttctgcg cgattgtca 
      gccatcctg cggacactg tggtgtcca agttgaacc 
      aggagaagg gctcgacgg ccctggagg ccgccaaaa 
      agctgggca tggccggac tgtggaaag ggctgacgc 
      ccaggattg taatgattg gtactttga ccgcattgc 
      aatggttta tctatgatg cgttcaaag tggcaatgc 
      gtatgccac gcccaccgc caccggaaa tgcccgagt 
      cgcttaaga agagattac aagccgagg gcaaattgt 
      aaagataat tacttgtat tttaattaa cagtatttc 
      cggatagat caagcgatt gtggcgttc tttgttatg 
      tcaaactct gttatctag actgtaaat agagctaaa 
      aaccgtaaa ttattctgt tttaaccaa taaaaggcc 
      ccgaaatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY