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Gene information
General information
TC033316
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015417 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 579411..582696
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033316
    iBeetle screen iB_08474

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033316 RA
      MFSSLLSRA MCQNGAPSE APQEMGLVK SLVAGRTIA 
AAAAASKTA EGDSCEFGS AHYFALCGM GGILSCGIT 
HTAIVPLDL VKCRIQVDP NKYKNVFTG FKVTMKEDG 
FKGLALGWA PTFFGYSAQ GLCKFGLYE VFKVFYTDL 
IGDENAFLY RTWLYLAAS ASAEFFADI ALSPMEAAK 
VKIQTSPGF ATTLRTAWP RIYSEEGIN GFYKSLVPL 
WMRQIPYTM MKFACFERT VELLYKHVV PKPRSECSK 
GEQLIVTFA AGYIAGVFC AIVSHPADT VVSKLNQEK 
GSTALEAAK KLGMAGLWK GLTPRIVMI GTLTALQWF 
IYDAFKVAM RMPRPPPPE MPESLKKRL QAEGKL
    CDS sequence
    • >TC033316 RA
      atgttctcg tcgttgttg tcgcgcgcg atgtgccag 
aatggggcc cccagcgag gccccgcaa gaaatgggg 
ctggtgaag tccctcgtt gctgggagg acgatagcg 
gcggcggcg gcagcctcc aagaccgcc gaaggagac 
agttgcgag ttcggctcg gcgcactat ttcgccctg 
tgcggaatg gggggcatc ctgtcttgt gggatcaca 
catacggca attgtaccc cttgatctg gtcaagtgc 
cggattcaa gtcgatccc aacaagtat aagaacgta 
ttcaccggg tttaaggtg accatgaaa gaggacggg 
ttcaagggc ttggccttg ggatgggcc cccacgttc 
tttgggtat tcggcgcaa gggttgtgc aagtttgga 
ttgtatgag gtttttaag gtattttac accgacttg 
attggcgac gaaaacgcc ttcttgtac cgcacttgg 
ctgtatttg gccgccagt gcttcagcg gaatttttc 
gccgacatt gcgctttcg ccaatggaa gccgccaaa 
gtgaaaatc caaacttcc cctggcttt gccaccact 
ttgcgcacc gcctggcct agaatctac tccgaggag 
ggcattaac ggcttctac aaaagcttg gtgcctctg 
tggatgcgc cagatcccc tacaccatg atgaagttc 
gcctgcttc gagcgaact gtcgaactc ctgtacaaa 
catgtggtc cccaaacca cgctcagag tgcagcaaa 
ggcgaacag ttgattgtc actttcgct gccggttac 
atagctggc gttttctgc gcgattgtc agccatcct 
gcggacact gtggtgtcc aagttgaac caggagaag 
ggctcgacg gccctggag gccgccaaa aagctgggc 
atggccgga ctgtggaaa gggctgacg cccaggatt 
gtaatgatt ggtactttg accgcattg caatggttt 
atctatgat gcgttcaaa gtggcaatg cgtatgcca 
cgcccaccg ccaccggaa atgcccgag tcgcttaag 
aagagatta caagccgag ggcaaattg taa
    mRNA sequence
    • >TC033316 RA
      tgataaatg tgggtgtta acatttttt ttcttattt 
tttcaattt tcaaaattt tcaaaagca gtgaagaaa 
taattgatt atttaccat tatgagact aaggcctta 
agggcaagt gacaaaccc gatgaacga atttcccca 
caaacaact gcctttgct tcaaaattt tatcacaaa 
aaaagactc aaatttatc attgggttt gttgcaaaa 
atagccaaa atcatgacc tgtctccaa tgcccctta 
cgcaacaat ttaattatg tcaaacacg tgaccttga 
tcaatgtcc acgtgcact tgcgcaata cagcgccat 
attaccacc agcggagac cacgagaac taccttcac 
ggggctgta gccattgtt tccaggccg ctttaacac 
cgatcacga tgttctcgt cgttgttgt cgcgcgcga 
tgtgccaga atggggccc ccagcgagg ccccgcaag 
aaatggggc tggtgaagt ccctcgttg ctgggagga 
cgatagcgg cggcggcgg cagcctcca agaccgccg 
aaggagaca gttgcgagt tcggctcgg cgcactatt 
tcgccctgt gcggaatgg ggggcatcc tgtcttgtg 
ggatcacac atacggcaa ttgtacccc ttgatctgg 
tcaagtgcc ggattcaag tcgatccca acaagtata 
agaacgtat tcaccgggt ttaaggtga ccatgaaag 
aggacgggt tcaagggct tggccttgg gatgggccc 
ccacgttct ttgggtatt cggcgcaag ggttgtgca 
agtttggat tgtatgagg tttttaagg tattttaca 
ccgacttga ttggcgacg aaaacgcct tcttgtacc 
gcacttggc tgtatttgg ccgccagtg cttcagcgg 
aatttttcg ccgacattg cgctttcgc caatggaag 
ccgccaaag tgaaaatcc aaacttccc ctggctttg 
ccaccactt tgcgcaccg cctggccta gaatctact 
ccgaggagg gcattaacg gcttctaca aaagcttgg 
tgcctctgt ggatgcgcc agatcccct acaccatga 
tgaagttcg cctgcttcg agcgaactg tcgaactcc 
tgtacaaac atgtggtcc ccaaaccac gctcagagt 
gcagcaaag gcgaacagt tgattgtca ctttcgctg 
ccggttaca tagctggcg ttttctgcg cgattgtca 
gccatcctg cggacactg tggtgtcca agttgaacc 
aggagaagg gctcgacgg ccctggagg ccgccaaaa 
agctgggca tggccggac tgtggaaag ggctgacgc 
ccaggattg taatgattg gtactttga ccgcattgc 
aatggttta tctatgatg cgttcaaag tggcaatgc 
gtatgccac gcccaccgc caccggaaa tgcccgagt 
cgcttaaga agagattac aagccgagg gcaaattgt 
aaagataat tacttgtat tttaattaa cagtatttc 
cggatagat caagcgatt gtggcgttc tttgttatg 
tcaaactct gttatctag actgtaaat agagctaaa 
aaccgtaaa ttattctgt tttaaccaa taaaaggcc 
ccgaaatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY