Status
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Gene information
General information
TC033308
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030331,TC030330,TC030329,TC015336
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 265311..271932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033308
    iBeetle screen iB_08466

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033308 RA
      MLKSAGLHP YTKLKIVKF FYHHYLNLF FFIFLLFLA 
      ILEVGVSVK CDIYRAIEA LSSVLFMTL TLFRYVVNY 
      RNKPSLAWL LEKRSNFWL LEHFEGQIR TDCAKIMHT 
      SSNFIRNYK NYAIVLAAV FYVQPFIFH ELQMKIYVP 
      EGWFYYLYL VYWYMTPPL FVSVYGVTS MFCAICIPV 
      TIQFKLLAH RIQNLDFKS EKFQRDLKH LVDYHNFLI 
      DYCTRINRY SNGVLLFEF FITISVCCI LIFIAANDY 
      PFVDKIKYA GFIVSQFLD TAIFCYNCE LISDASENV 
      GKAAYDSLW YESESKIRR SLILIIVRA QKKVTFSGY 
      GLVRINMMT FTQVFKATL SFTNVHKMS NKQLDPTYY 
      ILKIFKAAG VHPDVKVTP FLVFFFWMN CTIVSAVIV 
      LATIGAIFG ALDNDINTV VECLQSTFI YIHILGKHF 
      VLFYSKPIL SHLLAQRTH FLQLETFDL STIEQFQQL 
      LKKTSKFVN TFMFCTSCV VFSFYMQPY LTHGDLPVS 
      VYVPDGWYY YIHFGFWPL APCIVASIY GSDALFCAI 
      SVPVIVQFR LLARKIQNW KIENTKLNN QKSRKIFKK 
      NLKELVDHQ NFLFEYCNE MNKFNNGIF LNQFLLSVG 
      IICVQLFVV SQKGFKLPN KIKCVGYSF MEIIETAIF 
      CFNAELISD ASEDVGNAA YDSLWYESD DPEVRHAIT 
      LIIARSQNR IVFSGFGFV WINLKTFTQ IFKTALSFY 
      SYLHNVLLG KYPTLFFKK KTLKELLER QKQFWPIDN 
      VEPKLAKKF DQILTNTTK FIRYFIIVT FLVIMNFFL 
      QPILTGDLP VRVYVPSGW FHYINSVYW YLIPVIIGS 
      IYGSDLIFC SLCVPVIIQ FQLLAHKFE KFKPQQLKK 
      LVDYHNFLI KYCNDLNKY IEPIFLNQV IVATAIICM 
      QLFIVSQKE FLLPNKLKC LGYCFGEVI ETAIYCFNA 
      EMISDAAEK VGIAVYNSQ WYKVPRKSV VLVIAKTQK 
      KVVFNGLGL VAINLKTFT QIFKTALSF YSYLNTMIY 
      LSYENIYYT IDSILTIVL YLHIASKYV NLHLHKDTL 
      AMLIQERSK FWPIDTFEM TVRQKCVRI LTKSLTIIK 
      SYLGYSLLV VISFLIQPI ITGQLPVFM YVPRGTYYI 
      FFVIFMTIT PGIMSSIWG VDTLFFSIT TPVSIQFKL 
      LAHKFETID LKMDSKRVR HEFRKLVDY HNFLINYCQ 
      NINRMSSGI FLTQYLVAI ATSCMQLFI TSQPEFGLL 
      NKIKCLTYF IMQIIETGI YCFTAQLIS ESSENVGNA 
      VYKAPWYDF NCGTRRDIA LVIVRSQKK VVFNGLGLV 
      WIKMETFTK IFKTALSFY TYLNTMVYQ N
    CDS sequence
    • >TC033308 RA
      atgctgaaa agcgccggc ttgcaccct tataccaag 
      ctgaaaatt gtcaaattt ttctaccac cactatctc 
      aatttattc tttttcatt tttctacta tttttggca 
      attttggaa gttggcgtt tcagtcaag tgcgacatt 
      tatcgcgct attgaagcg ctgtcttca gttttgttc 
      atgaccttg acactcttt cgctacgtc gtcaattat 
      cgcaataaa ccatccctt gcctggctg ttggaaaag 
      cgctcaaac ttctggctt ttggagcac tttgagggc 
      caaattcgc accgattgt gccaaaata atgcacaca 
      agctcgaat tttatccga aattacaaa aattatgcc 
      atagtttta gccgcagtt ttttatgtc cagccgttt 
      attttccac gaactccaa atgaaaatc tacgtgcct 
      gaagggtgg ttttactac ttgtatttg gtttattgg 
      tacatgaca ccgcctttg ttcgtcagt gtttacgga 
      gtcacttcg atgttttgc gctatttgt attccagtc 
      acaattcag ttcaaactt ttggctcat cgtatccaa 
      aatttggac ttcaaaagc gaaaagttt caacgtgat 
      ttgaaacat ttggtcgat tatcataac tttctgatt 
      gattattgc acgagaatt aatcggtat tcgaacggg 
      gtgctactt tttgagttt tttatcacg atttcagtt 
      tgttgtatt ttgattttt atcgctgca aacgattat 
      ccgttcgta gataaaatc aagtatgca ggttttatc 
      gtgagtcag tttttggat actgccatc ttttgctac 
      aattgtgaa ctaatctca gacgcttca gagaacgtc 
      ggtaaagca gcttacgac tcattatgg tacgaatcc 
      gaaagcaaa attcgccga agtttgatc ttaattatc 
      gtacgagcc caaaaaaaa gtcactttc agtggctac 
      ggactggtc cgaatcaat atgatgacg ttcacacaa 
      gtttttaaa gccactctc tccttcacc aacgtccac 
      aaaatgtcg aataaacaa ctagaccca acttactat 
      attttgaaa atttttaaa gcagctggt gtgcacccg 
      gacgttaaa gtcacccca tttttggtc ttttttttc 
      tggatgaac tgtactatt gtgtctgct gttatagtt 
      ttggcaacc attggtgca attttcggc gctttagac 
      aacgatatt aacacagtg gttgaatgt ctccaaagt 
      acctttatt tacatacat attttaggc aaacacttc 
      gtcttgttc tacagcaaa cccatcctg tcccatctc 
      cttgcccaa cgcacccat ttcctccaa ttggagacg 
      ttcgactta agtaccatc gagcagttc caacaactg 
      ctcaaaaaa acctccaag ttcgtcaac accttcatg 
      ttttgcacc agctgtgtg gttttcagc ttctacatg 
      caaccatac ctcacccat ggcgatttg ccagtcagt 
      gtttacgta ccagatggg tggtactac tacatccat 
      tttgggttc tggcctttg gcaccatgc attgttgcc 
      agtatttac ggttcagat gcacttttt tgcgcaata 
      agtgttcca gttattgtg caatttcga cttttggca 
      cggaaaata caaaactgg aaaatcgaa aacaccaaa 
      cttaataat caaaagagt aggaaaatt ttcaaaaaa 
      aatttgaaa gagctagta gaccaccag aattttttg 
      tttgagtat tgtaacgaa atgaataag ttcaataac 
      ggcattttt ctcaaccag tttctgctt tcggttgga 
      attatttgt gtccaactg tttgtagtg tcacaaaaa 
      gggtttaaa ctgccaaat aaaattaaa tgtgtcggg 
      tattccttt atggaaatt atcgaaact gctattttt 
      tgcttcaat gctgaatta atctcagat gcgtccgaa 
      gacgtcgga aatgcggct tacgactcc ctctggtac 
      gaatcagac gatcccgaa gtacgacac gccataact 
      ctcataatt gcgcgttcg cagaatcgc atagttttc 
      agcggtttt ggtttcgtt tggatcaat ttgaaaact 
      ttcactcag atttttaaa accgccctc tcattctac 
      agttatctc cacaacgtt ttattgggt aaatacccg 
      acacttttc ttcaagaaa aaaacactg aaagagctg 
      ctagaacgc caaaaacaa ttctggcct attgataat 
      gttgaacca aaactcgcc aaaaaattt gaccagatt 
      ttgaccaac acaacgaaa tttattcgc tattttata 
      atcgttacg tttctagta ataatgaat ttttttcta 
      caaccgatt ctaacaggg gatttaccg gtacgtgtg 
      tacgtaccg tctggttgg ttccattac atcaattca 
      gtctactgg tacttgatt ccggtcata attggaagt 
      atctacggt tctgattta attttttgc tcactttgt 
      gttcccgtt attatacaa tttcaatta ctagcacac 
      aaattcgaa aagttcaaa ccccaacag ttgaaaaaa 
      ctcgtcgat tatcacaat tttctaata aaatattgc 
      aacgacttg aacaaatac attgaaccg atcttcctc 
      aatcaagtg atcgtagcc acggccata atctgcatg 
      cagttgttt atagtttcg caaaaggaa tttttactc 
      cccaacaaa ctaaaatgt ttgggttat tgttttggc 
      gaagtgatc gaaactgcg atttattgc ttcaatgcc 
      gaaatgatt tctgatgct gccgaaaaa gttggcatc 
      gctgtgtat aattcacag tggtacaaa gtaccacga 
      aaatcggtc gttttggtt attgccaag acgcagaaa 
      aaggtggtt tttaacgga cttggtttg gttgcaatt 
      aacttgaaa acgttcaca cagattttc aaaaccgcg 
      ctgtcgttt tatagttat ttgaacacg atgatttac 
      ctctcgtac gaaaatatc tactacacg attgacagt 
      attttgaca attgtgcta tatcttcat attgctagc 
      aaatatgtg aatttgcat ttgcataaa gacactcta 
      gctatgttg attcaagag cggtcaaaa ttttggcca 
      attgacact tttgaaatg acagtgcgt caaaaatgt 
      gtcagaatt ttaacaaaa agtttgact ataattaag 
      tcctatttg ggttatagt ctcttggtt gtgatcagt 
      tttctgata cagcccata atcacggga caattgcca 
      gtttttatg tacgtacca cgaggcaca tactatatt 
      ttttttgtc attttcatg acaatcaca ccaggcatt 
      atgtcaagt atttggggt gtcgatact ttgtttttt 
      tcaatcaca actccagtt tccattcag tttaaactg 
      ctcgctcac aagtttgag acaatcgac ctgaagatg 
      gattcgaaa cgggtgcgt cacgaattc cgcaaactg 
      gtcgattat cacaatttt ctgatcaat tattgccaa 
      aatatcaat cgaatgtcg agcggaatt ttcctgact 
      cagtatcta gtggccatt gctacgtcc tgtatgcag 
      ctcttcata acatcgcag ccagagttt ggtcttttg 
      aacaaaatc aagtgtttg acctatttt attatgcaa 
      atcattgag actggaatt tattgtttc acggcacag 
      ttgatttcc gaaagttcc gaaaatgtt ggtaatgca 
      gtttacaag gcaccttgg tacgacttt aattgtggt 
      actaggaga gatattgcg ttggttatt gtcaggtca 
      cagaaaaag gtagttttt aacggactt gggctggtt 
      tggatcaag atggagact tttacaaag atttttaag 
      acggctctc tctttctac acgtactta aacactatg 
      gtttaccaa aactag
    mRNA sequence
    • >TC033308 RA
      taaaatgtg ctcaattta aactaagtc acttccatg 
      taaactgaa acaattttt caggcccga aaccctctc 
      attctaccc cgaactgat gctgaaaag cgccggctt 
      gcaccctta taccaagct gaaaattgt caaattttt 
      ctaccacca ctatctcaa tttattctt tttcatttt 
      tctactatt tttggcaat tttggaagt tggcgtttc 
      agtcaagtg cgacattta tcgcgctat tgaagcgct 
      gtcttcagt tttgttcat gaccttgac actctttcg 
      ctacgtcgt caattatcg caataaacc atcccttgc 
      ctggctgtt ggaaaagcg ctcaaactt ctggctttt 
      ggagcactt tgagggcca aattcgcac cgattgtgc 
      caaaataat gcacacaag ctcgaattt tatccgaaa 
      ttacaaaaa ttatgccat agttttagc cgcagtttt 
      ttatgtcca gccgtttat tttccacga actccaaat 
      gaaaatcta cgtgcctga agggtggtt ttactactt 
      gtatttggt ttattggta catgacacc gcctttgtt 
      cgtcagtgt ttacggagt cacttcgat gttttgcgc 
      tatttgtat tccagtcac aattcagtt caaactttt 
      ggctcatcg tatccaaaa tttggactt caaaagcga 
      aaagtttca acgtgattt gaaacattt ggtcgatta 
      tcataactt tctgattga ttattgcac gagaattaa 
      tcggtattc gaacggggt gctactttt tgagttttt 
      tatcacgat ttcagtttg ttgtatttt gatttttat 
      cgctgcaaa cgattatcc gttcgtaga taaaatcaa 
      gtatgcagg ttttatcgt gagtcagtt tttggatac 
      tgccatctt ttgctacaa ttgtgaact aatctcaga 
      cgcttcaga gaacgtcgg taaagcagc ttacgactc 
      attatggta cgaatccga aagcaaaat tcgccgaag 
      tttgatctt aattatcgt acgagccca aaaaaaagt 
      cactttcag tggctacgg actggtccg aatcaatat 
      gatgacgtt cacacaagt ttttaaagc cactctctc 
      cttcaccaa cgtccacaa aatgtcgaa taaacaact 
      agacccaac ttactatat tttgaaaat ttttaaagc 
      agctggtgt gcacccgga cgttaaagt caccccatt 
      tttggtctt ttttttctg gatgaactg tactattgt 
      gtctgctgt tatagtttt ggcaaccat tggtgcaat 
      tttcggcgc tttagacaa cgatattaa cacagtggt 
      tgaatgtct ccaaagtac ctttattta catacatat 
      tttaggcaa acacttcgt cttgttcta cagcaaacc 
      catcctgtc ccatctcct tgcccaacg cacccattt 
      cctccaatt ggagacgtt cgacttaag taccatcga 
      gcagttcca acaactgct caaaaaaac ctccaagtt 
      cgtcaacac cttcatgtt ttgcaccag ctgtgtggt 
      tttcagctt ctacatgca accatacct cacccatgg 
      cgatttgcc agtcagtgt ttacgtacc agatgggtg 
      gtactacta catccattt tgggttctg gcctttggc 
      accatgcat tgttgccag tatttacgg ttcagatgc 
      acttttttg cgcaataag tgttccagt tattgtgca 
      atttcgact tttggcacg gaaaataca aaactggaa 
      aatcgaaaa caccaaact taataatca aaagagtag 
      gaaaatttt caaaaaaaa tttgaaaga gctagtaga 
      ccaccagaa ttttttgtt tgagtattg taacgaaat 
      gaataagtt caataacgg catttttct caaccagtt 
      tctgctttc ggttggaat tatttgtgt ccaactgtt 
      tgtagtgtc acaaaaagg gtttaaact gccaaataa 
      aattaaatg tgtcgggta ttcctttat ggaaattat 
      cgaaactgc tattttttg cttcaatgc tgaattaat 
      ctcagatgc gtccgaaga cgtcggaaa tgcggctta 
      cgactccct ctggtacga atcagacga tcccgaagt 
      acgacacgc cataactct cataattgc gcgttcgca 
      gaatcgcat agttttcag cggttttgg tttcgtttg 
      gatcaattt gaaaacttt cactcagat ttttaaaac 
      cgccctctc attctacag ttatctcca caacgtttt 
      attgggtaa atacccgac acttttctt caagaaaaa 
      aacactgaa agagctgct agaacgcca aaaacaatt 
      ctggcctat tgataatgt tgaaccaaa actcgccaa 
      aaaatttga ccagatttt gaccaacac aacgaaatt 
      tattcgcta ttttataat cgttacgtt tctagtaat 
      aatgaattt ttttctaca accgattct aacagggga 
      tttaccggt acgtgtgta cgtaccgtc tggttggtt 
      ccattacat caattcagt ctactggta cttgattcc 
      ggtcataat tggaagtat ctacggttc tgatttaat 
      tttttgctc actttgtgt tcccgttat tatacaatt 
      tcaattact agcacacaa attcgaaaa gttcaaacc 
      ccaacagtt gaaaaaact cgtcgatta tcacaattt 
      tctaataaa atattgcaa cgacttgaa caaatacat 
      tgaaccgat cttcctcaa tcaagtgat cgtagccac 
      ggccataat ctgcatgca gttgtttat agtttcgca 
      aaaggaatt tttactccc caacaaact aaaatgttt 
      gggttattg ttttggcga agtgatcga aactgcgat 
      ttattgctt caatgccga aatgatttc tgatgctgc 
      cgaaaaagt tggcatcgc tgtgtataa ttcacagtg 
      gtacaaagt accacgaaa atcggtcgt tttggttat 
      tgccaagac gcagaaaaa ggtggtttt taacggact 
      tggtttggt tgcaattaa cttgaaaac gttcacaca 
      gattttcaa aaccgcgct gtcgtttta tagttattt 
      gaacacgat gatttacct ctcgtacga aaatatcta 
      ctacacgat tgacagtat tttgacaat tgtgctata 
      tcttcatat tgctagcaa atatgtgaa tttgcattt 
      gcataaaga cactctagc tatgttgat tcaagagcg 
      gtcaaaatt ttggccaat tgacacttt tgaaatgac 
      agtgcgtca aaaatgtgt cagaatttt aacaaaaag 
      tttgactat aattaagtc ctatttggg ttatagtct 
      cttggttgt gatcagttt tctgataca gcccataat 
      cacgggaca attgccagt ttttatgta cgtaccacg 
      aggcacata ctatatttt ttttgtcat tttcatgac 
      aatcacacc aggcattat gtcaagtat ttggggtgt 
      cgatacttt gtttttttc aatcacaac tccagtttc 
      cattcagtt taaactgct cgctcacaa gtttgagac 
      aatcgacct gaagatgga ttcgaaacg ggtgcgtca 
      cgaattccg caaactggt cgattatca caattttct 
      gatcaatta ttgccaaaa tatcaatcg aatgtcgag 
      cggaatttt cctgactca gtatctagt ggccattgc 
      tacgtcctg tatgcagct cttcataac atcgcagcc 
      agagtttgg tcttttgaa caaaatcaa gtgtttgac 
      ctattttat tatgcaaat cattgagac tggaattta 
      ttgtttcac ggcacagtt gatttccga aagttccga 
      aaatgttgg taatgcagt ttacaaggc accttggta 
      cgactttaa ttgtggtac taggagaga tattgcgtt 
      ggttattgt caggtcaca gaaaaaggt agtttttaa 
      cggacttgg gctggtttg gatcaagat ggagacttt 
      tacaaagat ttttaagac ggctctctc tttctacac 
      gtacttaaa cactatggt ttaccaaaa ctagaaaaa 
      tgacacaaa ttgtattcg ttccgtgtt gtctgtatg 
      caaatagta ccgttctat aaatttgta attcaaatc 
      attgaactt ataaaataa agacacttg cattga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY