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Gene information
General information
TC033303 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 129113..130484
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033303
    iBeetle screen iB_08461

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033303 RA
      MREKLKKSL VLANNLADA TYKKLLENC 
FVTLTGFPE PHNISTLYN SKPDQINEL 
YAALLALTA NLIQTGQNK VNTSQFLNE 
CHFSASKTA LFLDFYEKN RCEIEVNLI 
NIGNHLPHV TDITWKIDY IIKSSYLDS 
SQGPLFRIG LQTEKYDCE NEGKKIDFI 
QFTCDSQEL QDLVNKLKD AVRHCQRIG 
SEH
    CDS sequence
    • >TC033303 RA
      atgcgagaa aaactcaaa aaatcgcta gttttggcc 
aacaattta gccgacgca acttacaaa aaactcctc 
gaaaattgt tttgtcacc ttgactggt tttccagaa 
cctcacaac atttcaacc ctttacaac tccaaaccc 
gaccaaatt aatgaatta tatgcagct ttattggcc 
ctaactgca aatttgatc caaaccgga caaaataag 
gtaaatacg agccaattt ttaaacgaa tgtcacttc 
agtgcgagc aaaacggcc ctatttttg gatttttac 
gaaaaaaat cgctgcgaa atcgaagtc aatcttatc 
aatattgga aatcacttg cctcatgtt acggacatt 
acgtggaaa attgattac ataattaag tcgagttat 
ttggacagt agtcaaggc ccgctcttc agaattgga 
ttacagacg gaaaaatat gattgtgaa aatgagggc 
aaaaaaatt gattttatt caattcact tgtgatagt 
caggaatta caggatttg gttaataaa ctcaaagac 
gctgtgagg cattgccaa agaatcgga agtgaacat 
taa
    mRNA sequence
    • >TC033303 RA
      tgaaaataa aaccggcaa aataacagt tggcaacac 
tgtccccag taaaattcg aggttagat gacctccgg 
aaaaatgcg agaaaaact caaaaaatc gctagtttt 
ggccaacaa tttagccga cgcaactta caaaaaact 
cctcgaaaa ttgttttgt caccttgac tggttttcc 
agaacctca caacatttc aacccttta caactccaa 
acccgacca aattaatga attatatgc agctttatt 
ggccctaac tgcaaattt gatccaaac cggacaaaa 
taaggtaaa tacgagcca atttttaaa cgaatgtca 
cttcagtgc gagcaaaac ggccctatt tttggattt 
ttacgaaaa aaatcgctg cgaaatcga agtcaatct 
tatcaatat tggaaatca cttgcctca tgttacgga 
cattacgtg gaaaattga ttacataat taagtcgag 
ttatttgga cagtagtca aggcccgct cttcagaat 
tggattaca gacggaaaa atatgattg tgaaaatga 
gggcaaaaa aattgattt tattcaatt cacttgtga 
tagtcagga attacagga tttggttaa taaactcaa 
agacgctgt gaggcattg ccaaagaat cggaagtga 
acattaagt gattacttt atcattcct ttttattga 
aatttattt gattattat gtaaatgtg gactagttg 
ataattatt tgtataaaa ggctttaca tacacctta 
aataaatct aaaaatgta tttgtttgt tttattttg 
tgcttttat cgcgtcagg taagctaaa aatttgaca 
aatggcata accttaaat aaaggggct ttctgcgcc 
ccctttgaa gacgtaacc gaggcaaca atgagcgaa 
gaaaacaac acacaacct gaaatttta ttacaagag 
gaagacaat gctgaaaca agtataact caaattttt 
aaatttgca attatacga gggtcactc gatttgtgt 
tgttccagc aaacgggaa aaatcctat acaggagtg 
gttcgaaaa tttgggcaa aaatatgtc gaccaatac 
gcaaaacac gatgaataa cataaataa ttggtttac 
acaattggc acaaaataa taatggtgg gcaaaattt 
acccactat gtatatggg ttcgttcca attggtcaa 
aatgcccat gcctcaaat tagcgacgt tgttaaagg 
aatgggaga tttggcgac ggtggttca aactttgaa 
taaattggg tggaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY