Status
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Gene information
General information
TC013601 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3245679..3250308
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013601
    iBeetle screen iB_08458

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013601 RA
      MSKRFSTGQ SNTLLNYFQ SPKSSNGKD VKQNGAGKR 
      DLVSNKDEG DVSPVQAKK RKKTTIIDS DSDDEHLPI 
      QPTSKKSKN GKKRPVSDS DSESSDSDS KPAEKSLKL 
      SASKTNEKS AAKAAPIVS DLNTNWLHN RLDFLQPDK 
      IRDVNKRRP DDPDYDPRT LYIPQSFLE KQTPAMRQW 
      WVLKSTHMD SVLFFKVGK FYELYHMDA VVGVTQLGF 
      SYMKGEFAH SGFPESAYH KMANALIEK GFKVARTEQ 
      TETPEMMAE RCKKQGRAT KFDKVVNRE ICQISTKAT 
      CVYTAQLPD AMHSQSCYM YAIAEKDVT GSQRRFGIC 
      FIDTSIGVF KLAEFDDDK HCSKLLVCL SEYPPGLIL 
      TERKKLSSK LKLILNTNY RDVRRESLA PESQFYGAS 
      TTIEKLLNG NYFRNESND LCLPEVLKN VTDGYNAKP 
      EFELMVKAL GGCLWYLHD SKLDIQVVS LGKFEIYQP 
      LEMNVREKS PRSCMILDS VTIVNLNLL GEENSLQKT 
      LDYCQTAFG KRLLTQWIC RPLCVVEKI KERQEAVQE 
      LVKNTSLLK DAQDVLKKL PDLERQLAK IHTYGNKFF 
      AQDHPDSRA VFYEAATYS KRRIGDLLK TLQGFELAQ 
      NLCPLFKGC QSSLLKRLT QFKPDGHYV DLTELLLFF 
      KHAFDQEEA QKEGKIIPK PGVDENYDQ AQDAIKAVT 
      KKLDEYLVE LQQFFGCKV TYFGSDKKR FQIDIPESH 
      TKKVTSEYQ LEGTKKGAK PSKRYSTSR SRQLLAEMM 
      KAESERAKV IQDLNRRIF QKFSEKREQ WEQAIDCIT 
      VLDVLCSLA QYACNFGQD ICIPEIEPM GNSDKIVIE 
      NGRHPCITN IDNFVPNDT KMGVADFAN ILLITGPNM 
      GGKSTLMRQ IAIICIMAH MGGYVPASG CTLSLIDRI 
      FTRLGAHDD IVQGQSTFL VELSEAAVM LHHATPHSL 
      VLLDELGRG TSTHDGNAI ATAYVEKLT TINCRTLFS 
      THYHSLVDH FEARRDVQL GHMACMVEN DDDPTEESV 
      TFLYKMARG RCPKSYGFN VARLSGLKH CIVSRGREI 
      SKQLENESK SRQVFRDLF TSSVATIRS AMSKLSLS
    CDS sequence
    • >TC013601 RA
      atgtcgaaa cggttcagc acgggccaa agtaacacc 
      cttctgaat tatttccaa tctccgaaa agctcaaat 
      ggtaaagac gtcaagcaa aacggcgcc gggaaaagg 
      gatcttgtt tctaataag gacgaggga gacgtatcc 
      ccagttcaa gccaagaaa cgcaagaaa acgacaatt 
      atcgacagt gactcagac gatgagcat ttgccgatc 
      cagccaact tccaagaag tcgaaaaac gggaagaaa 
      cggccggtg agcgactca gacagcgag tcttcggac 
      agcgacagc aagcccgcc gagaagtcg ctcaaactg 
      tccgcttcc aagacaaat gagaaaagc gcagccaaa 
      gcggcccca attgtcagc gatcttaac accaattgg 
      ttgcacaat cggcttgac ttcctccaa cccgataaa 
      atccgcgat gttaataag agacggccc gatgatcca 
      gactatgac cctcgcaca ctttacatt cctcagagc 
      ttcctggag aagcaaact ccggcaatg cgtcagtgg 
      tgggtcctg aaaagcacc cacatggac tcggttttg 
      ttcttcaaa gttggaaag ttctacgag ctttaccac 
      atggatgcc gttgtgggg gtcacacaa ctggggttt 
      tcctacatg aagggggag ttcgcccac tccggcttt 
      cccgaaagc gcctaccat aagatggca aatgcttta 
      atcgagaag gggtttaag gtggcaagg accgaacaa 
      accgaaacc cccgaaatg atggccgaa aggtgcaaa 
      aaacaggga cgtgccacc aaattcgat aaagtagtc 
      aatcgggaa atttgtcaa atttcaacg aaagccact 
      tgcgtctac accgcacag ttgcccgat gccatgcat 
      tcgcagtct tgttacatg tatgccatt gcagagaag 
      gacgtaact ggttcgcaa agaagattc ggaatttgt 
      tttattgat acgtccatt ggcgtcttc aaactggca 
      gagtttgat gacgacaaa cactgttca aagctttta 
      gtctgtctt tcggaatat ccaccagga ttaatcttg 
      actgagaga aagaagctg agctccaaa ctaaaacta 
      attttaaac accaactac agagatgtt cggcgagaa 
      tcgctggct cccgagagt caattttac ggtgccagc 
      accactatt gaaaaatta ctgaacggg aattatttt 
      cgtaacgaa agtaatgac ctgtgtttg cctgaagtc 
      ctcaaaaac gtcacagac ggatataac gctaagcca 
      gagtttgag ttaatggtg aaagcgtta ggtggctgc 
      ttgtggtac ttgcatgac tctaagctc gacattcag 
      gtcgtttcg ttggggaaa ttcgagatt taccagccg 
      ttggagatg aatgtgcgg gagaaaagc ccccgcagt 
      tgcatgatt ttggattct gtgacaatt gtcaacttg 
      aatctcttg ggtgaagag aacagtttg caaaaaacg 
      ttggattat tgtcagact gcgtttggc aaaaggttg 
      ctgacacag tggatctgt cggcctttg tgtgtcgtt 
      gagaaaatt aaagaacgg caagaggcg gttcaagag 
      ctggtcaaa aacacgtct ttgttgaaa gacgcacaa 
      gacgttctt aaaaaattg ccagatttg gagcgacaa 
      ttggccaag attcacact tacggtaac aaatttttt 
      gcccaagat caccccgac agtcgcgct gttttctac 
      gaagcggcc acttattct aaaaggcga attggcgac 
      ctcttgaaa actttgcaa gggtttgaa ctggcacag 
      aacttgtgc ccgttgttc aaaggctgc cagtcgtcg 
      ttacttaag cgcttgact caatttaaa cccgacggt 
      cattacgtt gatttgacc gaactgttg ttgtttttt 
      aaacacgcg tttgaccaa gaagaggcc cagaaagaa 
      ggtaaaata atcccaaag ccgggcgtt gacgagaat 
      tacgaccag gcacaagat gctatcaag gcagtaacg 
      aaaaaactc gatgagtat ttagtcgaa ctgcaacaa 
      tttttcggc tgcaaagtt acatacttc ggttcggat 
      aaaaaacgc ttccaaatt gatattccc gaaagtcac 
      accaagaaa gtaacaagt gaataccaa ctcgaaggg 
      actaaaaaa ggcgctaag ccctcaaaa cgttactca 
      acgtccaga agtcggcaa cttctagca gagatgatg 
      aaagcggag agcgagagg gcgaaagtt atccaagat 
      ttgaaccgg aggattttc cagaagttt agcgaaaag 
      cgcgagcag tgggagcaa gccattgat tgtattact 
      gtcttggac gtgttgtgt tctctagcg caatatgct 
      tgcaatttc ggacaggat atatgtatt cctgaaatt 
      gaaccaatg ggaaactca gataaaatt gtcattgaa 
      aatggtcgt cacccctgc attacaaac attgataac 
      ttcgttccc aacgacact aaaatggga gttgccgat 
      tttgcgaac attttgctc atcactgga cccaatatg 
      ggtggtaaa tccactctg atgcgtcag attgccatt 
      atttgtatt atggcacat atggggggt tacgtgcct 
      gcgtccggc tgtacttta tctctgatt gatcgaatt 
      tttactcgt ctgggggct catgacgat attgttcaa 
      ggccagtct acgttcttg gtagaactg tccgaagct 
      gcagttatg ttgcatcat gcgacgcca cattcgctg 
      gtcctgctt gatgagtta ggtagaggt acttccacg 
      cacgatgga aatgctata gctaccgcg tatgtggaa 
      aaactaaca acgattaat tgtcggacg ttgttttct 
      acgcactac cacagtctt gtggaccat tttgaggcg 
      aggcgagac gtgcagttg ggacatatg gcttgcatg 
      gtcgaaaac gacgacgat ccgactgaa gagtcggtt 
      actttcctg tataagatg gcaagaggg cgctgtccg 
      aagtcgtat ggtttcaac gtagcgcga ctctcgggc 
      ttgaaacat tgcattgtt tcacgaggg cgggaaata 
      tccaaacag ttggagaat gaatccaaa agtcgtcaa 
      gttttcagg gatttattc acatctagt gtggcaacg 
      attagatct gctatgtca aagttatct ctctcttaa
    mRNA sequence
    • >TC013601 RA
      tgtcggtcg ctagaaggc gcgcttttg ttgtgttgt 
      tggttgttt ggttaaaaa actatttgc tccgtgttg 
      tcaatccga aaattttgc acaaaatgt cgaaacggt 
      tcagcacgg gccaaagta acacccttc tgaattatt 
      tccaatctc cgaaaagct caaatggta aagacgtca 
      agcaaaacg gcgccggga aaagggatc ttgtttcta 
      ataaggacg agggagacg tatccccag ttcaagcca 
      agaaacgca agaaaacga caattatcg acagtgact 
      cagacgatg agcatttgc cgatccagc caacttcca 
      agaagtcga aaaacggga agaaacggc cggtgagcg 
      actcagaca gcgagtctt cggacagcg acagcaagc 
      ccgccgaga agtcgctca aactgtccg cttccaaga 
      caaatgaga aaagcgcag ccaaagcgg ccccaattg 
      tcagcgatc ttaacacca attggttgc acaatcggc 
      ttgacttcc tccaacccg ataaaatcc gcgatgtta 
      ataagagac ggcccgatg atccagact atgaccctc 
      gcacacttt acattcctc agagcttcc tggagaagc 
      aaactccgg caatgcgtc agtggtggg tcctgaaaa 
      gcacccaca tggactcgg ttttgttct tcaaagttg 
      gaaagttct acgagcttt accacatgg atgccgttg 
      tgggggtca cacaactgg ggttttcct acatgaagg 
      gggagttcg cccactccg gctttcccg aaagcgcct 
      accataaga tggcaaatg ctttaatcg agaaggggt 
      ttaaggtgg caaggaccg aacaaaccg aaacccccg 
      aaatgatgg ccgaaaggt gcaaaaaac agggacgtg 
      ccaccaaat tcgataaag tagtcaatc gggaaattt 
      gtcaaattt caacgaaag ccacttgcg tctacaccg 
      cacagttgc ccgatgcca tgcattcgc agtcttgtt 
      acatgtatg ccattgcag agaaggacg taactggtt 
      cgcaaagaa gattcggaa tttgtttta ttgatacgt 
      ccattggcg tcttcaaac tggcagagt ttgatgacg 
      acaaacact gttcaaagc ttttagtct gtctttcgg 
      aatatccac caggattaa tcttgactg agagaaaga 
      agctgagct ccaaactaa aactaattt taaacacca 
      actacagag atgttcggc gagaatcgc tggctcccg 
      agagtcaat tttacggtg ccagcacca ctattgaaa 
      aattactga acgggaatt attttcgta acgaaagta 
      atgacctgt gtttgcctg aagtcctca aaaacgtca 
      cagacggat ataacgcta agccagagt ttgagttaa 
      tggtgaaag cgttaggtg gctgcttgt ggtacttgc 
      atgactcta agctcgaca ttcaggtcg tttcgttgg 
      ggaaattcg agatttacc agccgttgg agatgaatg 
      tgcgggaga aaagccccc gcagttgca tgattttgg 
      attctgtga caattgtca acttgaatc tcttgggtg 
      aagagaaca gtttgcaaa aaacgttgg attattgtc 
      agactgcgt ttggcaaaa ggttgctga cacagtgga 
      tctgtcggc ctttgtgtg tcgttgaga aaattaaag 
      aacggcaag aggcggttc aagagctgg tcaaaaaca 
      cgtctttgt tgaaagacg cacaagacg ttcttaaaa 
      aattgccag atttggagc gacaattgg ccaagattc 
      acacttacg gtaacaaat tttttgccc aagatcacc 
      ccgacagtc gcgctgttt tctacgaag cggccactt 
      attctaaaa ggcgaattg gcgacctct tgaaaactt 
      tgcaagggt ttgaactgg cacagaact tgtgcccgt 
      tgttcaaag gctgccagt cgtcgttac ttaagcgct 
      tgactcaat ttaaacccg acggtcatt acgttgatt 
      tgaccgaac tgttgttgt tttttaaac acgcgtttg 
      accaagaag aggcccaga aagaaggta aaataatcc 
      caaagccgg gcgttgacg agaattacg accaggcac 
      aagatgcta tcaaggcag taacgaaaa aactcgatg 
      agtatttag tcgaactgc aacaatttt tcggctgca 
      aagttacat acttcggtt cggataaaa aacgcttcc 
      aaattgata ttcccgaaa gtcacacca agaaagtaa 
      caagtgaat accaactcg aagggacta aaaaaggcg 
      ctaagccct caaaacgtt actcaacgt ccagaagtc 
      ggcaacttc tagcagaga tgatgaaag cggagagcg 
      agagggcga aagttatcc aagatttga accggagga 
      ttttccaga agtttagcg aaaagcgcg agcagtggg 
      agcaagcca ttgattgta ttactgtct tggacgtgt 
      tgtgttctc tagcgcaat atgcttgca atttcggac 
      aggatatat gtattcctg aaattgaac caatgggaa 
      actcagata aaattgtca ttgaaaatg gtcgtcacc 
      cctgcatta caaacattg ataacttcg ttcccaacg 
      acactaaaa tgggagttg ccgattttg cgaacattt 
      tgctcatca ctggaccca atatgggtg gtaaatcca 
      ctctgatgc gtcagattg ccattattt gtattatgg 
      cacatatgg ggggttacg tgcctgcgt ccggctgta 
      ctttatctc tgattgatc gaattttta ctcgtctgg 
      gggctcatg acgatattg ttcaaggcc agtctacgt 
      tcttggtag aactgtccg aagctgcag ttatgttgc 
      atcatgcga cgccacatt cgctggtcc tgcttgatg 
      agttaggta gaggtactt ccacgcacg atggaaatg 
      ctatagcta ccgcgtatg tggaaaaac taacaacga 
      ttaattgtc ggacgttgt tttctacgc actaccaca 
      gtcttgtgg accattttg aggcgaggc gagacgtgc 
      agttgggac atatggctt gcatggtcg aaaacgacg 
      acgatccga ctgaagagt cggttactt tcctgtata 
      agatggcaa gagggcgct gtccgaagt cgtatggtt 
      tcaacgtag cgcgactct cgggcttga aacattgca 
      ttgtttcac gagggcggg aaatatcca aacagttgg 
      agaatgaat ccaaaagtc gtcaagttt tcagggatt 
      tattcacat ctagtgtgg caacgatta gatctgcta 
      tgtcaaagt tatctctct cttaatgca tttttatta 
      cttttttat ataccacag tgaataaac tattatttt 
      gata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY