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Gene information
General information
TC010608 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451776.1: 26466..32283
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010608
    iBeetle screen iB_08453

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010608 RA
      MDRTMYGKI VPGTLHPYQ CTLKNGTSP GCRRNREKI 
PIYVVEYHH EVLPHIYRN IGAKLLPLE GNTIVHFDS 
HPDMLIPKN MPESYVYKK EKLFEEISI ENWMLPGAY 
AGHFKTLWW IKPPWAHQI EDSDQKFLI GRNKNGHIR 
VNCKENYFV SECLFAKTE DLEGVREVS LRVATLGED 
FDRKVLGEV TGPYILDID LDFFSTSNP FKEIYNKAG 
TYNKLKEIY KFVKPSDDI FECVSRREE QIENLERIF 
KHLQDRKCL PEDVPSEVR QLRDELLKF YSEEEIDWE 
LVHDAGCTC DDTELPHHV ASSEELGVM FERFRGVLE 
GLATPPVVV TVSRSTEDD YTPSEDVEV IQESVLRIV 
REKFNCDEP VLDYSEENA E
    CDS sequence
    • >TC010608 RA
      atggatcgc acgatgtac ggcaaaatt gtccccggg 
actttacac ccctatcag tgcaccctc aaaaatggg 
acgtccccc ggatgtcgc cggaatcgc gaaaaaatt 
ccgatttat gtggtggag taccatcac gaggtcttg 
ccccatatt taccgcaat atcggggcc aagctgttg 
cctctggag ggcaacacc attgtgcat tttgactcg 
caccccgat atgttgatt cccaaaaat atgcccgag 
agttatgtg tacaagaag gagaaattg ttcgaggag 
attagtatc gaaaattgg atgttgcca ggggcttac 
gctggccat tttaagaca ctttggtgg attaagccc 
ccatgggct catcagatt gaagacagc gatcagaaa 
tttttgatt gggaggaac aaaaacggc cacattcgg 
gtcaattgt aaagagaat tatttcgtt tcggagtgt 
ttgttcgcc aaaactgag gacctggag ggtgttagg 
gaagtttcc ttaagggtg gcaacccta ggggaggat 
tttgatcga aaagttttg ggcgaagtc acgggaccg 
tacattctg gacatcgat ttggatttt ttcagtacg 
agcaacccc ttcaaggag atttacaac aaggctggg 
acttacaac aaattgaag gagatttac aaatttgtg 
aaacccagt gatgacatt ttcgagtgt gtgtcaagg 
cgggaagaa cagattgaa aatttggaa agaatcttc 
aagcatttg caagaccgg aaatgtctg cccgaagat 
gtgccgagc gaagtgcga cagttgaga gatgagctg 
ttgaagttt tattccgag gaggaaatc gactgggag 
ttggtgcat gatgccggt tgtacgtgt gacgatacg 
gagttgcct catcacgtg gcgagttct gaagaactg 
ggtgttatg tttgagcgg tttcggggg gttttggag 
ggtttggcg acgcctcca gttgttgtg actgtgtca 
agatcaacg gaggatgat tatacaccg agcgaggac 
gtggaggtg attcaagag tccgttttg aggattgtt 
agggaaaaa tttaattgt gatgaacca gttttggat 
tacagtgaa gaaaatgcc gaatag
    mRNA sequence
    • >TC010608 RA
      cttgttatg gtttttatc tctgggttg tggggattc 
ccgatcagg gacttcacc aacggtgac taaagttaa 
ataaaaaca agagcaacg aatttaatt ttatttttg 
tgctggatt ttgcagtct tcttagacg tccttatca 
ctatcagtt cttatcatg ttttattaa atcataact 
taacgtaat tttcgttac gcagttatc aagtcactt 
agatacggg gggcaactc agtcagtta attcgcaag 
gtttgtgcg ggaaaaaaa ttttggttt ttaaaattt 
tgcaaaact tgttgagag gcgctgccc gtccgttgt 
ttacttctt tcaaggttg cctacactg accgtctgg 
aatttcata tcttgacat ttgtgattc tgcgaaaat 
gcctaaata aggtcattt tcctaaaaa gtgccgata 
attcgtgat gtagttgag aagttttgc ttaaaatta 
gtattagaa cggcagctt attaccatg gatgaccta 
gacgtttga atttggcgc tcagttttt tcttttatc 
cgatcatgg atcgcacga tgtacggca aaattgtcc 
ccgggactt tacacccct atcagtgca ccctcaaaa 
atgggacgt cccccggat gtcgccgga atcgcgaaa 
aaattccga tttatgtgg tggagtacc atcacgagg 
tcttgcccc atatttacc gcaatatcg gggccaagc 
tgttgcctc tggagggca acaccattg tgcattttg 
actcgcacc ccgatatgt tgattccca aaaatatgc 
ccgagagtt atgtgtaca agaaggaga aattgttcg 
aggagatta gtatcgaaa attggatgt tgccagggg 
cttacgctg gccatttta agacacttt ggtggatta 
agcccccat gggctcatc agattgaag acagcgatc 
agaaatttt tgattggga ggaacaaaa acggccaca 
ttcgggtca attgtaaag agaattatt tcgtttcgg 
agtgtttgt tcgccaaaa ctgaggacc tggagggtg 
ttagggaag tttccttaa gggtggcaa ccctagggg 
aggattttg atcgaaaag ttttgggcg aagtcacgg 
gaccgtaca ttctggaca tcgatttgg attttttca 
gtacgagca accccttca aggagattt acaacaagg 
ctgggactt acaacaaat tgaaggaga tttacaaat 
ttgtgaaac ccagtgatg acattttcg agtgtgtgt 
caaggcggg aagaacaga ttgaaaatt tggaaagaa 
tcttcaagc atttgcaag accggaaat gtctgcccg 
aagatgtgc cgagcgaag tgcgacagt tgagagatg 
agctgttga agttttatt ccgaggagg aaatcgact 
gggagttgg tgcatgatg ccggttgta cgtgtgacg 
atacggagt tgcctcatc acgtggcga gttctgaag 
aactgggtg ttatgtttg agcggtttc ggggggttt 
tggagggtt tggcgacgc ctccagttg ttgtgactg 
tgtcaagat caacggagg atgattata caccgagcg 
aggacgtgg aggtgattc aagagtccg ttttgagga 
ttgttaggg aaaaattta attgtgatg aaccagttt 
tggattaca gtgaagaaa atgccgaat agatgtgtt 
gttagttga ttttttgtg aattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY