Status
Please wait ...
Gene information
General information
TC033259
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013023
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15014246..15022273
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033259
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033259 RA
      MNILFQGSG GKAPYYMGS TASLPRGAA LLRTYSPAV 
      GALPADRLK SLPTDGYMS SPERGSRYE DPYYSQYAS 
      RSGSITPVI DEEVSDTEL LDDSYSLYG VKLASGGPR 
      GGPRSPFPP PAAVPYDAT RLRVENMER QLANLTGLV 
      QKALTQTPT THLQVPGRE NYRTGDEFD KNSSSSSAS 
      LPDKSVSFE KSVSFSDEP PDMNSPKQH SPQHAADTK 
      PAKPAIKSS TLPRTSSQE RDRLKPAPP PKPASLSPG 
      QYDGRLLYR DLQLTPEMY NQLRGLQKK AKDLRAEVR 
      NLRRMSQAQ AHSVRETIR DTFIKIRAM LLAGGEQVW 
      QAGMDQERV RLSREEDLY KQEMLRLEK DLSDLESTV 
      EELRGNVIN RKTRVNMSD VENMALVLS KSSKTVADL 
      KLRFPGLQD GMKTFLSSE MEKVMREEK FLKEEPERL 
      ESALRRCKK LTGTLVTLK RLASVQEQR LPCSNSSTD 
      GRLSPTVTE TPPITPTSS SKPPSGARV GAVVVGGDS 
      VSSSDSSQR PENALDALL DELQTFSKP PSEEEPPPA 
      VPAKGVAPS HPPHMSEIG RKGSMDSAT FLPTQNLTV 
      STATGSLRR LHSYPSGSD TDNSPPIQP QKTLSKPPL 
      PERNAELLQ HITGRRVPP PPPPRTSSK SPLASPTSP 
      SLPPRSPAA GLQHIQNVQ NMVENKPQS QFEAATQNL 
      GLGTLRRNV PNRSSLKDK AQTLPRNVG SNLQVPPEN 
      EAISASNSS SCESVNSQD GTKKSRKEE LEQRHQELL 
      KKQKALQEQ YARLQQLQR GNNTLAVVP PTSELLKKT 
      GSESNLLQK MGLQMTTSQ ITGSLTNLP NTTANSLSK 
      NTSTNTTNS NNAEEQTTT TNKVYETDI L
    CDS sequence
    • >TC033259 RA
      atgaatata ttatttcag ggcagcggg ggcaaagcg 
      ccgtactac atgggctcg acggccagt ctcccccgg 
      ggcgccgcc ctcctgcgg acctactcg cccgccgtg 
      ggcgccctg cccgccgac cgcctcaag tcgctcccc 
      acagatggg tacatgtcg tcgcctgag aggggctcg 
      cggtacgag gacccctac tacagccaa tacgcctca 
      aggtcgggc tccatcacc cctgtcata gacgaagaa 
      gtcagtgac acagaattg ctggacgat tcgtactcc 
      ctttacggg gtcaagtta gcctcgggg gggcctcga 
      ggagggccc aggtccccc tttccgcct cctgctgcc 
      gttccatat gacgctaca cggttgcgt gtagaaaat 
      atggagcgt caacttgct aatttgaca ggtttggtc 
      cagaaggca ctcacacaa actcccaca acacattta 
      caagtacca ggaagggag aactaccgt acaggagac 
      gagttcgat aagaactcc agttcaagt tccgcctct 
      ttaccagat aagtcggtt tctttcgag aaatcggtc 
      tcattcagt gatgagccg cccgacatg aattcgccg 
      aaacagcac agtccacaa cacgcagcg gatactaaa 
      cctgccaaa cccgccatt aaatcatca actcttccg 
      cggacctcc tcacaggag agagatcgt ttaaaaccg 
      gcgcctccg ccaaaaccg gcatccttg tctccggga 
      caatacgac ggaaggctt ttgtatcgc gacctgcag 
      ttaacaccg gaaatgtac aaccagttg cgaggcttg 
      caaaagaaa gccaaagat ttgagagcg gaagtgagg 
      aatttgagg aggatgtcg caagcgcaa gcacactca 
      gtcagagaa acaattagg gacacgttt attaaaatt 
      agagcgatg cttttagct ggaggtgaa caagtgtgg 
      caagccggg atggaccaa gaacgggtc cgtttgagt 
      cgagaggaa gacctttat aaacaagaa atgctacgt 
      ttggagaag gatttgagt gatttagaa tcaacagtt 
      gaggaattg aggggtaat gtgattaat agaaagaca 
      agggttaac atgtcagat gttgaaaac atggcacta 
      gtcttaagt aaaagctca aaaacagtg gccgattta 
      aaattaaga tttcctggc ctacaagat gggatgaag 
      actttccta agttcggaa atggagaaa gtaatgagg 
      gaggaaaag ttcctcaag gaagagccc gaacggctg 
      gaaagtgcg ttaagacgg tgtaaaaag ctcacaggg 
      actctagtc actttaaaa agacttgca agtgttcaa 
      gagcaacgg cttccgtgc tcgaactca tcgactgat 
      ggtcgtctc tcgcccact gtgacggag acgcctccc 
      atcaccccc acgtcatca agcaagccc ccttccggt 
      gcacgtgtg ggggcggtc gtcgtcggg ggagactcc 
      gtcagctcc agcgacagt agccaacgt ccggagaac 
      gcactcgac gctctgtta gacgagctg cagaccttc 
      tccaagccc cctagcgag gaggagccg ccgcccgca 
      gtgcccgcc aaaggcgtc gcccccagc caccccccg 
      cacatgtcc gagatcggc cggaaaggc agcatggac 
      tcggccacg ttcctcccg acccagaac ctgacagtg 
      agcaccgcc accggcagt ctgcgaaga ctgcactcg 
      taccccagc ggctccgac accgacaac agccccccg 
      atccagccc cagaagacc ctcagcaag ccgccgctg 
      cccgaacgc aacgccgag ctgttgcag cacatcacg 
      gggcggcgc gtgccgcct ccgccgccc ccgcgcacc 
      agctccaag tcgccgctc gcctccccc acgtccccc 
      agtctgccg ccgcgctcg cccgccgcc ggcttgcag 
      cacatccag aacgtgcag aacatggtg gagaacaag 
      ccgcagtcg cagtttgaa gccgccaca caaaaccta 
      ggtttaggc actttgagg agaaacgtt cctaatagg 
      tctagtctc aaggataaa gcgcaaacg ctgccaagg 
      aatgtgggg tcgaatctg caagtgccc cccgaaaac 
      gaggcgatc agtgcctcc aatagcagc agttgcgag 
      agtgtcaac tcgcaggat gggacgaaa aagtcgcgg 
      aaggaggag ctggagcag aggcaccag gagttgttg 
      aagaagcag aaggcgctc caggagcag tatgccagg 
      ctgcagcag ctccaaagg gggaacaac acgttggcg 
      gtggtgcct cccacttcc gaattgttg aaaaagaca 
      gggagtgag tcgaatttg ttgcagaag atggggcta 
      caaatgact acctcacag atcaccggc tcgctgacg 
      aatcttcca aacactacc gctaattca cttagtaaa 
      aacacctcg accaatacc acgaatagt aacaacgcg 
      gaagaacag accaccacg actaataaa gtttacgaa 
      actgatata ctgtga
    mRNA sequence
    • >TC033259 RA
      agagaaact tgaacggaa acagcgatc aagtgcaat 
      gtcaagtcc cacgttttt aaatttcga tgacatttt 
      aatgttttg gggaaaaca gttcagtaa tttagcgga 
      actaagtgg atgaatata ttatttcag ggcagcggg 
      ggcaaagcg ccgtactac atgggctcg acggccagt 
      ctcccccgg ggcgccgcc ctcctgcgg acctactcg 
      cccgccgtg ggcgccctg cccgccgac cgcctcaag 
      tcgctcccc acagatggg tacatgtcg tcgcctgag 
      aggggctcg cggtacgag gacccctac tacagccaa 
      tacgcctca aggtcgggc tccatcacc cctgtcata 
      gacgaagaa gtcagtgac acagaattg ctggacgat 
      tcgtactcc ctttacggg gtcaagtta gcctcgggg 
      gggcctcga ggagggccc aggtccccc tttccgcct 
      cctgctgcc gttccatat gacgctaca cggttgcgt 
      gtagaaaat atggagcgt caacttgct aatttgaca 
      ggtttggtc cagaaggca ctcacacaa actcccaca 
      acacattta caagtacca ggaagggag aactaccgt 
      acaggagac gagttcgat aagaactcc agttcaagt 
      tccgcctct ttaccagat aagtcggtt tctttcgag 
      aaatcggtc tcattcagt gatgagccg cccgacatg 
      aattcgccg aaacagcac agtccacaa cacgcagcg 
      gatactaaa cctgccaaa cccgccatt aaatcatca 
      actcttccg cggacctcc tcacaggag agagatcgt 
      ttaaaaccg gcgcctccg ccaaaaccg gcatccttg 
      tctccggga caatacgac ggaaggctt ttgtatcgc 
      gacctgcag ttaacaccg gaaatgtac aaccagttg 
      cgaggcttg caaaagaaa gccaaagat ttgagagcg 
      gaagtgagg aatttgagg aggatgtcg caagcgcaa 
      gcacactca gtcagagaa acaattagg gacacgttt 
      attaaaatt agagcgatg cttttagct ggaggtgaa 
      caagtgtgg caagccggg atggaccaa gaacgggtc 
      cgtttgagt cgagaggaa gacctttat aaacaagaa 
      atgctacgt ttggagaag gatttgagt gatttagaa 
      tcaacagtt gaggaattg aggggtaat gtgattaat 
      agaaagaca agggttaac atgtcagat gttgaaaac 
      atggcacta gtcttaagt aaaagctca aaaacagtg 
      gccgattta aaattaaga tttcctggc ctacaagat 
      gggatgaag actttccta agttcggaa atggagaaa 
      gtaatgagg gaggaaaag ttcctcaag gaagagccc 
      gaacggctg gaaagtgcg ttaagacgg tgtaaaaag 
      ctcacaggg actctagtc actttaaaa agacttgca 
      agtgttcaa gagcaacgg cttccgtgc tcgaactca 
      tcgactgat ggtcgtctc tcgcccact gtgacggag 
      acgcctccc atcaccccc acgtcatca agcaagccc 
      ccttccggt gcacgtgtg ggggcggtc gtcgtcggg 
      ggagactcc gtcagctcc agcgacagt agccaacgt 
      ccggagaac gcactcgac gctctgtta gacgagctg 
      cagaccttc tccaagccc cctagcgag gaggagccg 
      ccgcccgca gtgcccgcc aaaggcgtc gcccccagc 
      caccccccg cacatgtcc gagatcggc cggaaaggc 
      agcatggac tcggccacg ttcctcccg acccagaac 
      ctgacagtg agcaccgcc accggcagt ctgcgaaga 
      ctgcactcg taccccagc ggctccgac accgacaac 
      agccccccg atccagccc cagaagacc ctcagcaag 
      ccgccgctg cccgaacgc aacgccgag ctgttgcag 
      cacatcacg gggcggcgc gtgccgcct ccgccgccc 
      ccgcgcacc agctccaag tcgccgctc gcctccccc 
      acgtccccc agtctgccg ccgcgctcg cccgccgcc 
      ggcttgcag cacatccag aacgtgcag aacatggtg 
      gagaacaag ccgcagtcg cagtttgaa gccgccaca 
      caaaaccta ggtttaggc actttgagg agaaacgtt 
      cctaatagg tctagtctc aaggataaa gcgcaaacg 
      ctgccaagg aatgtgggg tcgaatctg caagtgccc 
      cccgaaaac gaggcgatc agtgcctcc aatagcagc 
      agttgcgag agtgtcaac tcgcaggat gggacgaaa 
      aagtcgcgg aaggaggag ctggagcag aggcaccag 
      gagttgttg aagaagcag aaggcgctc caggagcag 
      tatgccagg ctgcagcag ctccaaagg gggaacaac 
      acgttggcg gtggtgcct cccacttcc gaattgttg 
      aaaaagaca gggagtgag tcgaatttg ttgcagaag 
      atggggcta caaatgact acctcacag atcaccggc 
      tcgctgacg aatcttcca aacactacc gctaattca 
      cttagtaaa aacacctcg accaatacc acgaatagt 
      aacaacgcg gaagaacag accaccacg actaataaa 
      gtttacgaa actgatata ctgtgacca agcactcaa 
      agacgattg caatctgta tgttgaatt tgttgcact 
      cgatactca tatcacgac tggattgtt attactttt 
      cctgtttac tctgattga atgtagtca gtgttttat 
      aacggttag gattttgtc ttttcgatc ttaatatac 
      ttatgaagg cgcaatttt accaaacta caataacgg 
      ctgtaattt cgtaaaact gcccaagga acgttctaa 
      aaatgtcac tttttgtta cctatcacg atttgttcc 
      gatctacac tgaactgta cataattac ataaagatt 
      gcaaaatgt atgacgcac ttattggaa aattaaaaa 
      caccactaa attcgttat tgagacaca ttgtacata 
      aaatgttaa tttggatcg ttccggttt tatttaact 
      tgcaggaac gtacagaag cacagaatc cagtaaaaa 
      cctatcctg tgaaacatt cctgcgaca aaatttgca 
      cacagagaa aagattttt gtgcttctg cacggtgca 
      ctgatttgg acgttgtta aatattgct caaaatata 
      atacttacc atgtaatca aagatgttg cacatcata 
      cttaactgt ggtgtttcc aaatgttgt gattaattt 
      gaacgggat tcttttggc tgcacttca gtaaaacat 
      ccacagatc cccaccaat attgtgatg aatcagttt 
      tgcattttt atatatgta cttagaaag cgcaaacgg 
      tccctcagc tgataatgt tactgttag aatttttta 
      tctggcata aggcctacc ggaaaatgc caaatttta 
      agactaacc gatcgttgt acgcccata aattccaca 
      gcagtatta aacagagtg aacattaaa ccaactgaa 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY