Status
Please wait ...
Gene information
General information
TC013064 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14505134..14507791
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013064
    iBeetle screen iB_08432

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013064 RA
      MSLDKAKFE LMQKSIQDE LKTIKAEEE ALFQDMSNM 
      LNQKLFLDS KIKGLAKLV PTLKVVKHE AQDLVNTIY 
      DISESSEKI SGKIRSLDT ARSRVNECQ QRVSDLIDL 
      EICSQGVQT AILDSDYEK GAAHVHRFL SIDQSLLQR 
      TATDVENVS GMLKSVRTL QDAASQLQA IVEHKFNEA 
      VKCEDLVAI ERFFKIFPL LGMHEEGIK ALCTYLRTK 
      VAATAEKNL KSAMTTPQS DKRHSVIYA DTLTLLFEG 
      LARVVDTHQ PLIETYYGP GRLLCAVSI LQKECDIQM 
      KRILTEWAR ARQINKKIQ LITDLSRMS TSSSFGKLE 
      KIDPKDLDI SIGEITLMH FRTELYIRF IQRKVVSDL 
      DVGVQDEDE KPKVLKSLE NLIKTSDLT HSKHELLLH 
      YLKLEQYFM EESVAKAVS MDSIEPSQQ TSSMVDDTF 
      FIVKKCIRR SISTGSLDG ICAIINNAC RILENDFCD 
      VLRARLRQG YPSGYLDLT QAYNVLQSS IQQGRLQAS 
      DTEQTRTAF IVALNNADS STEYVDALC DSVLKEIEQ 
      ALPLMNSNE RGKLESCLS GLSSVTTNL KEITEYGIQ 
      QLRVSAIKP RISPWVDNF TSINHQLSE DELSAYEAG 
      ESFVQTLIM NLETLLSLF KDVLTTANY DAFISVLTT 
      EVTQRLEKV VMKSSFNRL GGLVLDKEV RSLAGYITA 
      TTSWSVRDK FARLTQIAT ILNLEQVSE FYDYWGDHD 
      GALTWRLTP SEVRTVMGL RSDFKVEDI KRFKL
    CDS sequence
    • >TC013064 RA
      atgtcttta gataaggct aagttcgaa ctgatgcaa 
      aaaagcatc caggatgag cttaaaaca atcaaagcg 
      gaagaggag gcccttttc caagacatg tccaacatg 
      ttaaaccaa aaactgttc cttgatagc aaaatcaaa 
      ggccttgct aaactagtc cccacgctg aaagtggtg 
      aaacacgaa gcccaagat ctagtaaac accatttac 
      gatatttcg gaatcatct gagaaaatc agtggcaaa 
      attagatca cttgatacc gctcgaagc cgtgttaat 
      gaatgccag caacgcgtt agcgacctc attgacttg 
      gaaatatgc tctcaaggg gtccaaact gcgatactg 
      gactctgat tatgagaaa ggggcagct catgttcat 
      aggttccta tcaattgac cagtcgctc cttcagaga 
      accgcaacc gatgtcgag aacgtgtca ggaatgttg 
      aagtcagtg cggacttta caggacgct gcaagccaa 
      ttgcaggca atagttgag cataagttt aacgaagcg 
      gtcaagtgt gaggactta gttgcgatt gaacgcttc 
      tttaagatc tttcccttg ctgggaatg cacgaggaa 
      gggattaag gcgctctgt acttacctc agaaccaaa 
      gttgcagca accgctgaa aaaaatctg aaatcggca 
      atgacaacg ccccagtct gataaacgc cacagtgtt 
      atttatgcc gacacttta actctatta tttgagggg 
      ttggcacgt gttgttgac acgcaccag cctctgatt 
      gagacgtac tacggccca gggagactt ctctgcgcc 
      gtcagtatt ttacaaaaa gagtgcgac attcaaatg 
      aaaagaatc ttgacagag tgggctagg gcgcggcaa 
      attaacaaa aaaattcag ctcattacg gacttgtcc 
      agaatgagc accagttcc agttttggc aaactggaa 
      aaaatcgat cccaaagat ttggatatt tccatcgga 
      gagataact ctcatgcat ttcaggacc gagctttac 
      atcaggttc atacaacgc aaagttgtg tcagaccta 
      gacgtgggc gtccaggac gaagacgag aagccaaaa 
      gtgctaaag tcgctggaa aacctaata aaaaccagc 
      gacttgacc cactcgaag cacgaactg ttgttacac 
      tacttaaaa cttgagcag tattttatg gaggaatcg 
      gttgcgaaa gccgtaagt atggatagt attgaaccg 
      tcgcaacaa acgtccagt atggtcgat gacacgttt 
      ttcatagtc aaaaaatgc atacgtcgt tcaatatcg 
      accggaagt ttggacgga atctgtgcc attataaat 
      aacgcgtgc cgaatcctc gaaaacgat ttttgtgac 
      gttttgagg gcgcgttta aggcaaggc tacccttcg 
      ggttatctc gacttgact caggcctac aacgtgttg 
      caaagttcc atacaacag ggccggctc caggccagt 
      gacactgag cagacaagg actgcgttt atcgtggct 
      ttgaataat gctgatagt agcactgaa tacgttgat 
      gcgttgtgt gatagcgtt ttgaaggaa attgaacaa 
      gcccttcct ttgatgaac agcaacgaa cgcgggaaa 
      ctcgagagt tgcctctct ggcctgtct tcagtcacg 
      acaaatctg aaggaaatc accgagtat ggtattcag 
      cagttgcgc gtcagcgca attaaacca cgaatcagt 
      ccttgggtc gacaacttc accagtata aaccatcag 
      ttatccgag gacgaactg tcggcgtac gaagcgggc 
      gaatcgttc gtccaaacg cttattatg aaccttgaa 
      acgctcttg tccctgttt aaagatgtt ttgacaacg 
      gcgaattac gatgcgttt atttcggtt ttaacgacg 
      gaagttacc caaagactg gagaaagtc gtaatgaaa 
      tcgtcgttt aataggttg ggaggtttg gtattagat 
      aaagaagtt aggtcattg gcgggttac atcacagcg 
      acgacttcg tggtccgtg agagacaaa ttcgcgcgt 
      ttgactcaa atcgccaca attttaaac ttggagcaa 
      gtgtcggag ttttacgat tattggggc gatcacgat 
      ggggcgctt acgtggcgt ttgacgccg tctgaagtc 
      cggactgtg atggggcta aggtcggat tttaaggtc 
      gaggatata aaaagattc aagctgtga
    mRNA sequence
    • >TC013064 RA
      caaaacaat aaacattga aaaaatgtc tttagataa 
      ggctaagtt cgaactgat gcaaaaaag catccagga 
      tgagcttaa aacaatcaa agcggaaga ggaggccct 
      tttccaaga catgtccaa catgttaaa ccaaaaact 
      gttccttga tagcaaaat caaaggcct tgctaaact 
      agtccccac gctgaaagt ggtgaaaca cgaagccca 
      agatctagt aaacaccat ttacgatat ttcggaatc 
      atctgagaa aatcagtgg caaaattag atcacttga 
      taccgctcg aagccgtgt taatgaatg ccagcaacg 
      cgttagcga cctcattga cttggaaat atgctctca 
      aggggtcca aactgcgat actggactc tgattatga 
      gaaaggggc agctcatgt tcataggtt cctatcaat 
      tgaccagtc gctccttca gagaaccgc aaccgatgt 
      cgagaacgt gtcaggaat gttgaagtc agtgcggac 
      tttacagga cgctgcaag ccaattgca ggcaatagt 
      tgagcataa gtttaacga agcggtcaa gtgtgagga 
      cttagttgc gattgaacg cttctttaa gatctttcc 
      cttgctggg aatgcacga ggaagggat taaggcgct 
      ctgtactta cctcagaac caaagttgc agcaaccgc 
      tgaaaaaaa tctgaaatc ggcaatgac aacgcccca 
      gtctgataa acgccacag tgttattta tgccgacac 
      tttaactct attatttga ggggttggc acgtgttgt 
      tgacacgca ccagcctct gattgagac gtactacgg 
      cccagggag acttctctg cgccgtcag tattttaca 
      aaaagagtg cgacattca aatgaaaag aatcttgac 
      agagtgggc tagggcgcg gcaaattaa caaaaaaat 
      tcagctcat tacggactt gtccagaat gagcaccag 
      ttccagttt tggcaaact ggaaaaaat cgatcccaa 
      agatttgga tatttccat cggagagat aactctcat 
      gcatttcag gaccgagct ttacatcag gttcataca 
      acgcaaagt tgtgtcaga cctagacgt gggcgtcca 
      ggacgaaga cgagaagcc aaaagtgct aaagtcgct 
      ggaaaacct aataaaaac cagcgactt gacccactc 
      gaagcacga actgttgtt acactactt aaaacttga 
      gcagtattt tatggagga atcggttgc gaaagccgt 
      aagtatgga tagtattga accgtcgca acaaacgtc 
      cagtatggt cgatgacac gtttttcat agtcaaaaa 
      atgcatacg tcgttcaat atcgaccgg aagtttgga 
      cggaatctg tgccattat aaataacgc gtgccgaat 
      cctcgaaaa cgatttttg tgacgtttt gagggcgcg 
      tttaaggca aggctaccc ttcgggtta tctcgactt 
      gactcaggc ctacaacgt gttgcaaag ttccataca 
      acagggccg gctccaggc cagtgacac tgagcagac 
      aaggactgc gtttatcgt ggctttgaa taatgctga 
      tagtagcac tgaatacgt tgatgcgtt gtgtgatag 
      cgttttgaa ggaaattga acaagccct tcctttgat 
      gaacagcaa cgaacgcgg gaaactcga gagttgcct 
      ctctggcct gtcttcagt cacgacaaa tctgaagga 
      aatcaccga gtatggtat tcagcagtt gcgcgtcag 
      cgcaattaa accacgaat cagtccttg ggtcgacaa 
      cttcaccag tataaacca tcagttatc cgaggacga 
      actgtcggc gtacgaagc gggcgaatc gttcgtcca 
      aacgcttat tatgaacct tgaaacgct cttgtccct 
      gtttaaaga tgttttgac aacggcgaa ttacgatgc 
      gtttatttc ggttttaac gacggaagt tacccaaag 
      actggagaa agtcgtaat gaaatcgtc gtttaatag 
      gttgggagg tttggtatt agataaaga agttaggtc 
      attggcggg ttacatcac agcgacgac ttcgtggtc 
      cgtgagaga caaattcgc gcgtttgac tcaaatcgc 
      cacaatttt aaacttgga gcaagtgtc ggagtttta 
      cgattattg gggcgatca cgatggggc gcttacgtg 
      gcgtttgac gccgtctga agtccggac tgtgatggg 
      gctaaggtc ggattttaa ggtcgagga tataaaaag 
      attcaagct gtgagtgtt ggtggtaat aaaagtgca 
      gttttaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY