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Gene information
General information
TC013084 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14248453..14249347
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013084
    iBeetle screen iB_08426

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013084 RA
      MKRLCFYFP TILIIISGE NPCLAEEDE SRIVGGFEA 
NKADYPYAV SLRDPNNHH FCGGTLIDH EHVVTAAHC 
VAGGRSNVV VVGSDSLDK GGSTHKVIS TTVHPEYDP 
KLVVNDIAL LKIEPVTSY KFSFPVRMQ SNLSDYENP 
CYVMGWGLT EAGGKLSNK FKVAEVHPV SPTHCEEEW 
KEAYNPNVI CTTSDGNSA CQGDSGGPL ICDEKFTGI 
VSFGKPCAT GKPDVFTSV FAYNEWIDK NME
    • >TC013084 RB
      MKRLCFYFP TILIIISGE NPCLAAEED ESRIVGGFE 
ANKADYPYA VSLRDPNNH HFCGGTLID HEHVVTAAH 
CVAGGRSNV VVVGSDSLD KGGSTHKVI STTVHPEYD 
PKLVVNDIA LLKIEPVTS YKFSFPVRM QSNLSDYEN 
PCYVMGWGL TEAGGKLSN KFKVAEVHP VSPTHCEEE 
WKEAYNPNV ICTTSDGNS ACQGDSGGP LICDEKFTG 
IVSFGKPCA TGKPDVFTS VFAYNEWID KNME
    • >TC013084 RC
      MQSNLSDYE NPCYVMGWG LTEAGGKLS NKFKVAEVH 
PVSPTHCEE EWKEAYNPN VICTTSDGN SACQGDSGG 
PLICDEKFT GIVSFGKPC ATGKPDVFT SVFAYNEWI 
DKNME
    CDS sequence
    • >TC013084 RA
      atgaagcgt ttgtgtttt tacttcccc acgatttta 
attataatt tctggagaa aatccgtgt ttggcagaa 
gaagacgaa tcgcgaatt gtgggcgga tttgaggca 
aacaaagcc gactaccct tacgctgtg tccctgcgg 
gacccgaac aaccaccac ttttgtggg ggcacctta 
atcgaccac gaacacgtc gtgaccgcc gcccattgc 
gtcgctggc ggccgctcg aacgtcgta gtcgtgggc 
agtgactct ctagacaag ggtggaagc acccacaag 
gtcatttcc acgacggtt caccccgaa tacgaccca 
aaattggtc gtaaacgac atagcgctg ttgaaaata 
gagccggtc acgtcgtac aaattttca tttccggtc 
agaatgcaa tcaaattta tcggactat gaaaatccg 
tgctacgtc atgggctgg ggcttaacg gaagccggg 
ggaaaactc tccaataaa ttcaaagtg gcagaagtg 
catcctgtg agtcccaca cactgcgag gaagaatgg 
aaggaggcg tataatccc aacgtgatt tgtaccacc 
agcgatgga aattccgcc tgtcagggg gattcgggg 
gggccgtta atttgcgat gagaagttt accggaatt 
gtgtctttt gggaagccg tgcgctacg ggaaagccg 
gatgtgttt acctcggtt tttgcctac aacgagtgg 
attgacaaa aatatggaa tga
    • >TC013084 RB
      atgaagcgt ttgtgtttt tacttcccc acgatttta 
attataatt tctggagaa aatccgtgt ttggcagca 
gaagaagac gaatcgcga attgtgggc ggatttgag 
gcaaacaaa gccgactac ccttacgct gtgtccctg 
cgggacccg aacaaccac cacttttgt gggggcacc 
ttaatcgac cacgaacac gtcgtgacc gccgcccat 
tgcgtcgct ggcggccgc tcgaacgtc gtagtcgtg 
ggcagtgac tctctagac aagggtgga agcacccac 
aaggtcatt tccacgacg gttcacccc gaatacgac 
ccaaaattg gtcgtaaac gacatagcg ctgttgaaa 
atagagccg gtcacgtcg tacaaattt tcatttccg 
gtcagaatg caatcaaat ttatcggac tatgaaaat 
ccgtgctac gtcatgggc tggggctta acggaagcc 
gggggaaaa ctctccaat aaattcaaa gtggcagaa 
gtgcatcct gtgagtccc acacactgc gaggaagaa 
tggaaggag gcgtataat cccaacgtg atttgtacc 
accagcgat ggaaattcc gcctgtcag ggggattcg 
ggggggccg ttaatttgc gatgagaag tttaccgga 
attgtgtct tttgggaag ccgtgcgct acgggaaag 
ccggatgtg tttacctcg gtttttgcc tacaacgag 
tggattgac aaaaatatg gaatga
    • >TC013084 RC
      atgcaatca aatttatcg gactatgaa aatccgtgc 
tacgtcatg ggctggggc ttaacggaa gccggggga 
aaactctcc aataaattc aaagtggca gaagtgcat 
cctgtgagt cccacacac tgcgaggaa gaatggaag 
gaggcgtat aatcccaac gtgatttgt accaccagc 
gatggaaat tccgcctgt cagggggat tcggggggg 
ccgttaatt tgcgatgag aagtttacc ggaattgtg 
tcttttggg aagccgtgc gctacggga aagccggat 
gtgtttacc tcggttttt gcctacaac gagtggatt 
gacaaaaat atggaatga
    mRNA sequence
    • >TC013084 RA
      caagtacaa aatgaagcg tttgtgttt ttacttccc 
cacgatttt aattataat ttctggaga aaatccgtg 
tttggcaga agaagacga atcgcgaat tgtgggcgg 
atttgaggc aaacaaagc cgactaccc ttacgctgt 
gtccctgcg ggacccgaa caaccacca cttttgtgg 
gggcacctt aatcgacca cgaacacgt cgtgaccgc 
cgcccattg cgtcgctgg cggccgctc gaacgtcgt 
agtcgtggg cagtgactc tctagacaa gggtggaag 
cacccacaa ggtcatttc cacgacggt tcaccccga 
atacgaccc aaaattggt cgtaaacga catagcgct 
gttgaaaat agagccggt cacgtcgta caaattttc 
atttccggt cagaatgca atcaaattt atcggacta 
tgaaaatcc gtgctacgt catgggctg gggcttaac 
ggaagccgg gggaaaact ctccaataa attcaaagt 
ggcagaagt gcatcctgt gagtcccac acactgcga 
ggaagaatg gaaggaggc gtataatcc caacgtgat 
ttgtaccac cagcgatgg aaattccgc ctgtcaggg 
ggattcggg ggggccgtt aatttgcga tgagaagtt 
taccggaat tgtgtcttt tgggaagcc gtgcgctac 
gggaaagcc ggatgtgtt tacctcggt ttttgccta 
caacgagtg gattgacaa aaatatgga atgattttt 
cttcgaaaa taaaaactt gaagaaatt
    • >TC013084 RB
      caagtacaa aatgaagcg tttgtgttt ttacttccc 
cacgatttt aattataat ttctggaga aaatccgtg 
tttggcagc agaagaaga cgaatcgcg aattgtggg 
cggatttga ggcaaacaa agccgacta cccttacgc 
tgtgtccct gcgggaccc gaacaacca ccacttttg 
tgggggcac cttaatcga ccacgaaca cgtcgtgac 
cgccgccca ttgcgtcgc tggcggccg ctcgaacgt 
cgtagtcgt gggcagtga ctctctaga caagggtgg 
aagcaccca caaggtcat ttccacgac ggttcaccc 
cgaatacga cccaaaatt ggtcgtaaa cgacatagc 
gctgttgaa aatagagcc ggtcacgtc gtacaaatt 
ttcatttcc ggtcagaat gcaatcaaa tttatcgga 
ctatgaaaa tccgtgcta cgtcatggg ctggggctt 
aacggaagc cgggggaaa actctccaa taaattcaa 
agtggcaga agtgcatcc tgtgagtcc cacacactg 
cgaggaaga atggaagga ggcgtataa tcccaacgt 
gatttgtac caccagcga tggaaattc cgcctgtca 
gggggattc gggggggcc gttaatttg cgatgagaa 
gtttaccgg aattgtgtc ttttgggaa gccgtgcgc 
tacgggaaa gccggatgt gtttacctc ggtttttgc 
ctacaacga gtggattga caaaaatat ggaatgatt 
tttcttcga aaataaaaa cttgaagaa att
    • >TC013084 RC
      ccacgaaca cgtcgtgac cgccgccca ttgcgtcgc 
tggcggccg ctcgaacgt cgtagtcgt gggcagtga 
ctctctaga caagggtgg aagcaccca caaggtcat 
ttccacgac ggttcaccc cgaatacga cccaaaatt 
ggtcgtaaa cgacatagc gctgttgaa aatagagcc 
ggtcacgtc gtacaaatt ttcatttcc ggtcagaat 
gcaatcaaa tttatcgga ctatgaaaa tccgtgcta 
cgtcatggg ctggggctt aacggaagc cgggggaaa 
actctccaa taaattcaa agtggcaga agtgcatcc 
tgtgagtcc cacacactg cgaggaaga atggaagga 
ggcgtataa tcccaacgt gatttgtac caccagcga 
tggaaattc cgcctgtca gggggattc gggggggcc 
gttaatttg cgatgagaa gtttaccgg aattgtgtc 
ttttgggaa gccgtgcgc tacgggaaa gccggatgt 
gtttacctc ggtttttgc ctacaacga gtggattga 
caaaaatat ggaatgatt tttcttcga aaataaaaa 
cttgaagaa att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY