GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08426 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC013084 RA
MKRLCFYFP TILIIISGE NPCLAEEDE SRIVGGFEA NKADYPYAV SLRDPNNHH FCGGTLIDH EHVVTAAHC VAGGRSNVV VVGSDSLDK GGSTHKVIS TTVHPEYDP KLVVNDIAL LKIEPVTSY KFSFPVRMQ SNLSDYENP CYVMGWGLT EAGGKLSNK FKVAEVHPV SPTHCEEEW KEAYNPNVI CTTSDGNSA CQGDSGGPL ICDEKFTGI VSFGKPCAT GKPDVFTSV FAYNEWIDK NME
- >TC013084 RB
MKRLCFYFP TILIIISGE NPCLAAEED ESRIVGGFE ANKADYPYA VSLRDPNNH HFCGGTLID HEHVVTAAH CVAGGRSNV VVVGSDSLD KGGSTHKVI STTVHPEYD PKLVVNDIA LLKIEPVTS YKFSFPVRM QSNLSDYEN PCYVMGWGL TEAGGKLSN KFKVAEVHP VSPTHCEEE WKEAYNPNV ICTTSDGNS ACQGDSGGP LICDEKFTG IVSFGKPCA TGKPDVFTS VFAYNEWID KNME
- >TC013084 RC
MQSNLSDYE NPCYVMGWG LTEAGGKLS NKFKVAEVH PVSPTHCEE EWKEAYNPN VICTTSDGN SACQGDSGG PLICDEKFT GIVSFGKPC ATGKPDVFT SVFAYNEWI DKNME
- >TC013084 RA
atgaagcgt ttgtgtttt tacttcccc acgatttta attataatt tctggagaa aatccgtgt ttggcagaa gaagacgaa tcgcgaatt gtgggcgga tttgaggca aacaaagcc gactaccct tacgctgtg tccctgcgg gacccgaac aaccaccac ttttgtggg ggcacctta atcgaccac gaacacgtc gtgaccgcc gcccattgc gtcgctggc ggccgctcg aacgtcgta gtcgtgggc agtgactct ctagacaag ggtggaagc acccacaag gtcatttcc acgacggtt caccccgaa tacgaccca aaattggtc gtaaacgac atagcgctg ttgaaaata gagccggtc acgtcgtac aaattttca tttccggtc agaatgcaa tcaaattta tcggactat gaaaatccg tgctacgtc atgggctgg ggcttaacg gaagccggg ggaaaactc tccaataaa ttcaaagtg gcagaagtg catcctgtg agtcccaca cactgcgag gaagaatgg aaggaggcg tataatccc aacgtgatt tgtaccacc agcgatgga aattccgcc tgtcagggg gattcgggg gggccgtta atttgcgat gagaagttt accggaatt gtgtctttt gggaagccg tgcgctacg ggaaagccg gatgtgttt acctcggtt tttgcctac aacgagtgg attgacaaa aatatggaa tga
- >TC013084 RB
atgaagcgt ttgtgtttt tacttcccc acgatttta attataatt tctggagaa aatccgtgt ttggcagca gaagaagac gaatcgcga attgtgggc ggatttgag gcaaacaaa gccgactac ccttacgct gtgtccctg cgggacccg aacaaccac cacttttgt gggggcacc ttaatcgac cacgaacac gtcgtgacc gccgcccat tgcgtcgct ggcggccgc tcgaacgtc gtagtcgtg ggcagtgac tctctagac aagggtgga agcacccac aaggtcatt tccacgacg gttcacccc gaatacgac ccaaaattg gtcgtaaac gacatagcg ctgttgaaa atagagccg gtcacgtcg tacaaattt tcatttccg gtcagaatg caatcaaat ttatcggac tatgaaaat ccgtgctac gtcatgggc tggggctta acggaagcc gggggaaaa ctctccaat aaattcaaa gtggcagaa gtgcatcct gtgagtccc acacactgc gaggaagaa tggaaggag gcgtataat cccaacgtg atttgtacc accagcgat ggaaattcc gcctgtcag ggggattcg ggggggccg ttaatttgc gatgagaag tttaccgga attgtgtct tttgggaag ccgtgcgct acgggaaag ccggatgtg tttacctcg gtttttgcc tacaacgag tggattgac aaaaatatg gaatga
- >TC013084 RC
atgcaatca aatttatcg gactatgaa aatccgtgc tacgtcatg ggctggggc ttaacggaa gccggggga aaactctcc aataaattc aaagtggca gaagtgcat cctgtgagt cccacacac tgcgaggaa gaatggaag gaggcgtat aatcccaac gtgatttgt accaccagc gatggaaat tccgcctgt cagggggat tcggggggg ccgttaatt tgcgatgag aagtttacc ggaattgtg tcttttggg aagccgtgc gctacggga aagccggat gtgtttacc tcggttttt gcctacaac gagtggatt gacaaaaat atggaatga
- >TC013084 RA
caagtacaa aatgaagcg tttgtgttt ttacttccc cacgatttt aattataat ttctggaga aaatccgtg tttggcaga agaagacga atcgcgaat tgtgggcgg atttgaggc aaacaaagc cgactaccc ttacgctgt gtccctgcg ggacccgaa caaccacca cttttgtgg gggcacctt aatcgacca cgaacacgt cgtgaccgc cgcccattg cgtcgctgg cggccgctc gaacgtcgt agtcgtggg cagtgactc tctagacaa gggtggaag cacccacaa ggtcatttc cacgacggt tcaccccga atacgaccc aaaattggt cgtaaacga catagcgct gttgaaaat agagccggt cacgtcgta caaattttc atttccggt cagaatgca atcaaattt atcggacta tgaaaatcc gtgctacgt catgggctg gggcttaac ggaagccgg gggaaaact ctccaataa attcaaagt ggcagaagt gcatcctgt gagtcccac acactgcga ggaagaatg gaaggaggc gtataatcc caacgtgat ttgtaccac cagcgatgg aaattccgc ctgtcaggg ggattcggg ggggccgtt aatttgcga tgagaagtt taccggaat tgtgtcttt tgggaagcc gtgcgctac gggaaagcc ggatgtgtt tacctcggt ttttgccta caacgagtg gattgacaa aaatatgga atgattttt cttcgaaaa taaaaactt gaagaaatt
- >TC013084 RB
caagtacaa aatgaagcg tttgtgttt ttacttccc cacgatttt aattataat ttctggaga aaatccgtg tttggcagc agaagaaga cgaatcgcg aattgtggg cggatttga ggcaaacaa agccgacta cccttacgc tgtgtccct gcgggaccc gaacaacca ccacttttg tgggggcac cttaatcga ccacgaaca cgtcgtgac cgccgccca ttgcgtcgc tggcggccg ctcgaacgt cgtagtcgt gggcagtga ctctctaga caagggtgg aagcaccca caaggtcat ttccacgac ggttcaccc cgaatacga cccaaaatt ggtcgtaaa cgacatagc gctgttgaa aatagagcc ggtcacgtc gtacaaatt ttcatttcc ggtcagaat gcaatcaaa tttatcgga ctatgaaaa tccgtgcta cgtcatggg ctggggctt aacggaagc cgggggaaa actctccaa taaattcaa agtggcaga agtgcatcc tgtgagtcc cacacactg cgaggaaga atggaagga ggcgtataa tcccaacgt gatttgtac caccagcga tggaaattc cgcctgtca gggggattc gggggggcc gttaatttg cgatgagaa gtttaccgg aattgtgtc ttttgggaa gccgtgcgc tacgggaaa gccggatgt gtttacctc ggtttttgc ctacaacga gtggattga caaaaatat ggaatgatt tttcttcga aaataaaaa cttgaagaa att
- >TC013084 RC
ccacgaaca cgtcgtgac cgccgccca ttgcgtcgc tggcggccg ctcgaacgt cgtagtcgt gggcagtga ctctctaga caagggtgg aagcaccca caaggtcat ttccacgac ggttcaccc cgaatacga cccaaaatt ggtcgtaaa cgacatagc gctgttgaa aatagagcc ggtcacgtc gtacaaatt ttcatttcc ggtcagaat gcaatcaaa tttatcgga ctatgaaaa tccgtgcta cgtcatggg ctggggctt aacggaagc cgggggaaa actctccaa taaattcaa agtggcaga agtgcatcc tgtgagtcc cacacactg cgaggaaga atggaagga ggcgtataa tcccaacgt gatttgtac caccagcga tggaaattc cgcctgtca gggggattc gggggggcc gttaatttg cgatgagaa gtttaccgg aattgtgtc ttttgggaa gccgtgcgc tacgggaaa gccggatgt gtttacctc ggtttttgc ctacaacga gtggattga caaaaatat ggaatgatt tttcttcga aaataaaaa cttgaagaa att
