Status
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Gene information
General information
TC014691 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13481733..13486187
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014691
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08414

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014691 RA
      MCLFSKKSV HYLLTIRTR NYYYRGRFL TTAVDPPKS 
FKEIPGPLS LPLVGTLYL YFPFIGRYQ FDRLHKNAL 
KNFQLYGPI IREEIVPGE HIVWLGDPD DIAKMFHTE 
GTYPYRKSH LTLEKYRLD RPHIYNSGG LLPTNGPEW 
SRIRKVFQK GLSGPTEAL SFIKGSDDV ISEWLDTRF 
KKIHKETSN MDFLQELSR LFLELIGVA AFDIRFQSF 
HDDELDPCS KSTKLLESA FVTNSTILK TDNGPQLWR 
KFETPAYRR LRKAQELME SVAIDLVAL KLSTFKEKT 
SNPPTLLER YLASASLDF KDIIGVVCD FLLAGMDTT 
TYSSSFLLY HLATNPSTQ DALYEEACR LLPNPAAPL 
TTEKYKQAE YAKCAVKES LRLRPISIG VGRQLTTDV 
VFSGYKVPS GTVVVTLNQ VLSRMEKYF PEPDSFKPE 
RWMKNDPSY VQTHPYLVI PFGHGQRSC IARRFAEQN 
MVILILKLA RKYKLRWNG SEIDSKSLL INKPDGPIL 
LSFEPR
    CDS sequence
    • >TC014691 RA
      atgtgttta ttcagcaaa aaaagtgtt cattatctc 
ttaacaata agaacaaga aattattat tatcgcgga 
aggttttta actactgct gttgatccg ccaaagagc 
tttaaagaa ataccagga ccgctctcg cttcctctg 
gtggggacc ctctatctt tatttccca tttatcggt 
cgttatcag ttcgataga ttacacaaa aatgccctt 
aaaaacttc caactttac ggaccaatt atacgggag 
gagattgtc ccaggggaa catattgtc tggttgggg 
gatcctgac gacattgcc aaaatgttc cacacggag 
ggcacctac ccctacagg aagagccac ctcaccttg 
gagaaatac agactagat aggccccac atctacaac 
tcaggcggt ttgctccca acaaatgga cccgaatgg 
tcaagaatc cgaaaagtg ttccagaaa ggtctctct 
ggcccaacc gaggctctt agttttatc aaaggctca 
gatgacgtg atatctgag tggttagat actcgcttc 
aagaaaatt cacaaagag acgtcaaac atggatttt 
cttcaagaa ctctcaagg ttattttta gagctgata 
ggcgttgcc gcttttgat ataagattt caaagtttt 
cacgatgat gaattagac ccgtgctcc aaaagtacg 
aaattatta gaatcagcc tttgttact aatagtaca 
attttaaag acggataat gggccccaa ttgtggcgc 
aaattcgaa acaccggcc tacaggagg ctgagaaaa 
gcgcaggaa ttaatggag agcgttgcg attgatttg 
gtggctttg aagttgtcc accttcaag gaaaaaaca 
tcaaatcct cccactttg cttgagcga tacctcgcc 
tctgcctct ctggacttc aaggacatt ataggcgta 
gtgtgcgat tttttgttg gcaggaatg gacactacc 
acatactcc tcaagtttc ctgctttac cacttagcc 
accaaccct tcaactcaa gacgcactt tacgaggag 
gcttgccgt ctcctcccg aaccctgcg gcccctcta 
actacagaa aaatacaaa caagcagaa tatgcaaaa 
tgtgctgtg aaagaatct ctaagattg cggccgatt 
tcgatcggt gtcggccgc cagttgact acagatgtt 
gttttttca gggtacaaa gtgccttca gggacggtt 
gtagtgact ttgaaccaa gttttgagc cggatggag 
aaatatttt ccggagcct gattcattt aagcctgaa 
cgttggatg aaaaatgac ccttcctac gtccagacg 
cacccgtat cttgtgata cctttcggg catgggcaa 
aggtcctgc attgcgaga aggtttgcc gaacagaat 
atggttatt ttaattcta aagttggcg agaaaatac 
aaattgaga tggaacgga tctgaaatc gactcaaaa 
tccctactg atcaataaa ccagatgga ccaatttta 
ttgtctttt gagccaaga tag
    mRNA sequence
    • >TC014691 RA
      ggtcttttg aacgatagt tttcctgag ttgtcaaaa 
atgactact taacctgaa tttaaattt gctttattt 
tcttaaaaa aagtaaatg caaaattgg aacttcaag 
tagtagggc gcaaaagtg gtaatttcg tattaaaat 
taaatagaa attgtttca gaacatgtc tgaacgaga 
ggttatttt gaaagctgc tctaacctc tcgcttttt 
ccttatcac aattcaata cgtctccaa tttactgat 
acctgacca cgaagctga cgcttacac ctatccaaa 
aaatgaaga taatggaat atttgataa actagtcac 
tcacttact gggcggttt tgtgagatg ggacgccca 
tcattttat gcatattgc aatttttgg ctacactta 
tgaacacac gttagcatt tttcccact ttttccata 
ttcagctaa accagttgt ggcaatttt aagttattt 
gcttccgtt tctttcaac tgattcatt gactctcat 
cacgttaat tacagtcaa tattttttg tttcgcaca 
atcagcgct gtgcctcta tcaattatc agccttaat 
aatgaacaa agatttggc cttgaaagc tgctatttt 
gttctgttg caatcccgc gtctgtatt tttcacagc 
cccgtattc agaatcact attctgcta cttgacttt 
taaatcgat gctaaattc ggtcctctt gtataaaaa 
atacaaaaa tcagggtta tttactgag tgatgtcgc 
ctacattat tccaatttg tctcagcac atgcacaag 
gtcgaatgg ggtcctaaa cattggatt tctagccta 
tgcgttaat ctgtctctt ccttgaaaa aattaaatt 
ggaaaaaca aatatgtaa cctcctgtt atgccttgc 
caaatttat tacattagc cgcggtttt agttttaat 
ttgcctagt gccatttat ttgcttcca gttttatgg 
ctaccaaac attttgtaa aaattttaa aagttatca 
agtccctct tgttttggg ccacaaaaa cattgataa 
ttttgtact acctgggac tttttcaca acacaatca 
gtcctggtg ttacaagaa aatgccatt ggcctactc 
ttacatcca gtctcacta ttggaaagt cggtttttt 
gcagtatta cgaacttaa acaaattcc taatttttt 
gctcgcaag tccgatcat tttaattat attaggaca 
ctcgttaca ttttcttaa agagtttcc taaatcaat 
tagtaaatt attcaactt tgatttaat ttccgtaaa 
gtcagtgcg cactaaaaa gtttttccc ggttatggc 
ggtgtttat cgttcacgc cagtgttct gaccctttt 
ttgtgtctt caatatcca cgtccaagt aaccacacg 
aatgttgtg ttccgcaag ccctgttat ttattggtt 
ttgttcgta ctacgtaac ggatgttaa tttattcaa 
aaatttgat tgcaagaaa atgtaactg gggcgagtt 
gttggtact tagctattt tctcgggta ttatttcgt 
agggaccgt aatgttttg taatttcct gccctggac 
ttgactatt aaacgattt gaaccacgc gctgttttt 
ttcaaaact tcaacctca aaattttta ttaattttt 
gtgttaatt ttattattg ttgaaattt attgcgtga 
cattacttg tgtgataac gttgcaact tggtttaga 
ttatttacg cgttgttat tggtgtgtg tgaatgcat 
ttggttgaa tcgcctcaa aatatgcat ccccactat 
gcataattt taacatgtt tttctttat attctttat 
tccgatttt gacccggct ttgcacctc tgccagatc 
tcaagcata tgtaaatca ttcaatgtg ttaaaagta 
ccatcaatt ttcttgtta tatccatta agtgccaat 
taattttat caaatatgt gtttattca gcaaaaaaa 
gtgttcatt atctcttaa caataagaa caagaaatt 
attattatc gcggaaggt ttttaacta ctgctgttg 
atccgccaa agagcttta aagaaatac caggaccgc 
tctcgcttc ctctggtgg ggaccctct atctttatt 
tcccattta tcggtcgtt atcagttcg atagattac 
acaaaaatg cccttaaaa acttccaac tttacggac 
caattatac gggaggaga ttgtcccag gggaacata 
ttgtctggt tgggggatc ctgacgaca ttgccaaaa 
tgttccaca cggagggca cctacccct acaggaaga 
gccacctca ccttggaga aatacagac tagataggc 
cccacatct acaactcag gcggtttgc tcccaacaa 
atggacccg aatggtcaa gaatccgaa aagtgttcc 
agaaaggtc tctctggcc caaccgagg ctcttagtt 
ttatcaaag gctcagatg acgtgatat ctgagtggt 
tagatactc gcttcaaga aaattcaca aagagacgt 
caaacatgg attttcttc aagaactct caaggttat 
ttttagagc tgataggcg ttgccgctt ttgatataa 
gatttcaaa gttttcacg atgatgaat tagacccgt 
gctccaaaa gtacgaaat tattagaat cagcctttg 
ttactaata gtacaattt taaagacgg ataatgggc 
cccaattgt ggcgcaaat tcgaaacac cggcctaca 
ggaggctga gaaaagcgc aggaattaa tggagagcg 
ttgcgattg atttggtgg ctttgaagt tgtccacct 
tcaaggaaa aaacatcaa atcctccca ctttgcttg 
agcgatacc tcgcctctg cctctctgg acttcaagg 
acattatag gcgtagtgt gcgattttt tgttggcag 
gaatggaca ctaccacat actcctcaa gtttcctgc 
tttaccact tagccacca acccttcaa ctcaagacg 
cactttacg aggaggctt gccgtctcc tcccgaacc 
ctgcggccc ctctaacta cagaaaaat acaaacaag 
cagaatatg caaaatgtg ctgtgaaag aatctctaa 
gattgcggc cgatttcga tcggtgtcg gccgccagt 
tgactacag atgttgttt tttcagggt acaaagtgc 
cttcaggga cggttgtag tgactttga accaagttt 
tgagccgga tggagaaat attttccgg agcctgatt 
catttaagc ctgaacgtt ggatgaaaa atgaccctt 
cctacgtcc agacgcacc cgtatcttg tgatacctt 
tcgggcatg ggcaaaggt cctgcattg cgagaaggt 
ttgccgaac agaatatgg ttattttaa ttctaaagt 
tggcgagaa aatacaaat tgagatgga acggatctg 
aaatcgact caaaatccc tactgatca ataaaccag 
atggaccaa ttttattgt cttttgagc caagatagt 
atgtatgag tatatttgc aagtatttt tacatgtaa 
ttattgcca ctaacgaaa tatattttt ataagtatt 
atggtaatg catgaatga acctatcaa aaacgtaca 
aattaaatt gcaaaaatt ggactaaac cagcgaggg 
gtcctatag gtagtgtag agtgcggtg caatggtgg 
caggataat taatcagaa aattatcac ggttttgta 
ggtcgttcc gtatataaa aagtaaaca cttcatgta 
taaggcatg aagtaatga taaatagca cacttgcat 
ggacctaca attaattat ggtgttatt gtaaaatta 
gttacaaat tacttacca ttgtgtagc accgttgaa 
cgtagagat ggaactat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY