Status
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Gene information
General information
TC014691 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13481733..13486187
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014691
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08414

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014691 RA
      MCLFSKKSV HYLLTIRTR NYYYRGRFL TTAVDPPKS 
      FKEIPGPLS LPLVGTLYL YFPFIGRYQ FDRLHKNAL 
      KNFQLYGPI IREEIVPGE HIVWLGDPD DIAKMFHTE 
      GTYPYRKSH LTLEKYRLD RPHIYNSGG LLPTNGPEW 
      SRIRKVFQK GLSGPTEAL SFIKGSDDV ISEWLDTRF 
      KKIHKETSN MDFLQELSR LFLELIGVA AFDIRFQSF 
      HDDELDPCS KSTKLLESA FVTNSTILK TDNGPQLWR 
      KFETPAYRR LRKAQELME SVAIDLVAL KLSTFKEKT 
      SNPPTLLER YLASASLDF KDIIGVVCD FLLAGMDTT 
      TYSSSFLLY HLATNPSTQ DALYEEACR LLPNPAAPL 
      TTEKYKQAE YAKCAVKES LRLRPISIG VGRQLTTDV 
      VFSGYKVPS GTVVVTLNQ VLSRMEKYF PEPDSFKPE 
      RWMKNDPSY VQTHPYLVI PFGHGQRSC IARRFAEQN 
      MVILILKLA RKYKLRWNG SEIDSKSLL INKPDGPIL 
      LSFEPR
    CDS sequence
    • >TC014691 RA
      atgtgttta ttcagcaaa aaaagtgtt cattatctc 
      ttaacaata agaacaaga aattattat tatcgcgga 
      aggttttta actactgct gttgatccg ccaaagagc 
      tttaaagaa ataccagga ccgctctcg cttcctctg 
      gtggggacc ctctatctt tatttccca tttatcggt 
      cgttatcag ttcgataga ttacacaaa aatgccctt 
      aaaaacttc caactttac ggaccaatt atacgggag 
      gagattgtc ccaggggaa catattgtc tggttgggg 
      gatcctgac gacattgcc aaaatgttc cacacggag 
      ggcacctac ccctacagg aagagccac ctcaccttg 
      gagaaatac agactagat aggccccac atctacaac 
      tcaggcggt ttgctccca acaaatgga cccgaatgg 
      tcaagaatc cgaaaagtg ttccagaaa ggtctctct 
      ggcccaacc gaggctctt agttttatc aaaggctca 
      gatgacgtg atatctgag tggttagat actcgcttc 
      aagaaaatt cacaaagag acgtcaaac atggatttt 
      cttcaagaa ctctcaagg ttattttta gagctgata 
      ggcgttgcc gcttttgat ataagattt caaagtttt 
      cacgatgat gaattagac ccgtgctcc aaaagtacg 
      aaattatta gaatcagcc tttgttact aatagtaca 
      attttaaag acggataat gggccccaa ttgtggcgc 
      aaattcgaa acaccggcc tacaggagg ctgagaaaa 
      gcgcaggaa ttaatggag agcgttgcg attgatttg 
      gtggctttg aagttgtcc accttcaag gaaaaaaca 
      tcaaatcct cccactttg cttgagcga tacctcgcc 
      tctgcctct ctggacttc aaggacatt ataggcgta 
      gtgtgcgat tttttgttg gcaggaatg gacactacc 
      acatactcc tcaagtttc ctgctttac cacttagcc 
      accaaccct tcaactcaa gacgcactt tacgaggag 
      gcttgccgt ctcctcccg aaccctgcg gcccctcta 
      actacagaa aaatacaaa caagcagaa tatgcaaaa 
      tgtgctgtg aaagaatct ctaagattg cggccgatt 
      tcgatcggt gtcggccgc cagttgact acagatgtt 
      gttttttca gggtacaaa gtgccttca gggacggtt 
      gtagtgact ttgaaccaa gttttgagc cggatggag 
      aaatatttt ccggagcct gattcattt aagcctgaa 
      cgttggatg aaaaatgac ccttcctac gtccagacg 
      cacccgtat cttgtgata cctttcggg catgggcaa 
      aggtcctgc attgcgaga aggtttgcc gaacagaat 
      atggttatt ttaattcta aagttggcg agaaaatac 
      aaattgaga tggaacgga tctgaaatc gactcaaaa 
      tccctactg atcaataaa ccagatgga ccaatttta 
      ttgtctttt gagccaaga tag
    mRNA sequence
    • >TC014691 RA
      ggtcttttg aacgatagt tttcctgag ttgtcaaaa 
      atgactact taacctgaa tttaaattt gctttattt 
      tcttaaaaa aagtaaatg caaaattgg aacttcaag 
      tagtagggc gcaaaagtg gtaatttcg tattaaaat 
      taaatagaa attgtttca gaacatgtc tgaacgaga 
      ggttatttt gaaagctgc tctaacctc tcgcttttt 
      ccttatcac aattcaata cgtctccaa tttactgat 
      acctgacca cgaagctga cgcttacac ctatccaaa 
      aaatgaaga taatggaat atttgataa actagtcac 
      tcacttact gggcggttt tgtgagatg ggacgccca 
      tcattttat gcatattgc aatttttgg ctacactta 
      tgaacacac gttagcatt tttcccact ttttccata 
      ttcagctaa accagttgt ggcaatttt aagttattt 
      gcttccgtt tctttcaac tgattcatt gactctcat 
      cacgttaat tacagtcaa tattttttg tttcgcaca 
      atcagcgct gtgcctcta tcaattatc agccttaat 
      aatgaacaa agatttggc cttgaaagc tgctatttt 
      gttctgttg caatcccgc gtctgtatt tttcacagc 
      cccgtattc agaatcact attctgcta cttgacttt 
      taaatcgat gctaaattc ggtcctctt gtataaaaa 
      atacaaaaa tcagggtta tttactgag tgatgtcgc 
      ctacattat tccaatttg tctcagcac atgcacaag 
      gtcgaatgg ggtcctaaa cattggatt tctagccta 
      tgcgttaat ctgtctctt ccttgaaaa aattaaatt 
      ggaaaaaca aatatgtaa cctcctgtt atgccttgc 
      caaatttat tacattagc cgcggtttt agttttaat 
      ttgcctagt gccatttat ttgcttcca gttttatgg 
      ctaccaaac attttgtaa aaattttaa aagttatca 
      agtccctct tgttttggg ccacaaaaa cattgataa 
      ttttgtact acctgggac tttttcaca acacaatca 
      gtcctggtg ttacaagaa aatgccatt ggcctactc 
      ttacatcca gtctcacta ttggaaagt cggtttttt 
      gcagtatta cgaacttaa acaaattcc taatttttt 
      gctcgcaag tccgatcat tttaattat attaggaca 
      ctcgttaca ttttcttaa agagtttcc taaatcaat 
      tagtaaatt attcaactt tgatttaat ttccgtaaa 
      gtcagtgcg cactaaaaa gtttttccc ggttatggc 
      ggtgtttat cgttcacgc cagtgttct gaccctttt 
      ttgtgtctt caatatcca cgtccaagt aaccacacg 
      aatgttgtg ttccgcaag ccctgttat ttattggtt 
      ttgttcgta ctacgtaac ggatgttaa tttattcaa 
      aaatttgat tgcaagaaa atgtaactg gggcgagtt 
      gttggtact tagctattt tctcgggta ttatttcgt 
      agggaccgt aatgttttg taatttcct gccctggac 
      ttgactatt aaacgattt gaaccacgc gctgttttt 
      ttcaaaact tcaacctca aaattttta ttaattttt 
      gtgttaatt ttattattg ttgaaattt attgcgtga 
      cattacttg tgtgataac gttgcaact tggtttaga 
      ttatttacg cgttgttat tggtgtgtg tgaatgcat 
      ttggttgaa tcgcctcaa aatatgcat ccccactat 
      gcataattt taacatgtt tttctttat attctttat 
      tccgatttt gacccggct ttgcacctc tgccagatc 
      tcaagcata tgtaaatca ttcaatgtg ttaaaagta 
      ccatcaatt ttcttgtta tatccatta agtgccaat 
      taattttat caaatatgt gtttattca gcaaaaaaa 
      gtgttcatt atctcttaa caataagaa caagaaatt 
      attattatc gcggaaggt ttttaacta ctgctgttg 
      atccgccaa agagcttta aagaaatac caggaccgc 
      tctcgcttc ctctggtgg ggaccctct atctttatt 
      tcccattta tcggtcgtt atcagttcg atagattac 
      acaaaaatg cccttaaaa acttccaac tttacggac 
      caattatac gggaggaga ttgtcccag gggaacata 
      ttgtctggt tgggggatc ctgacgaca ttgccaaaa 
      tgttccaca cggagggca cctacccct acaggaaga 
      gccacctca ccttggaga aatacagac tagataggc 
      cccacatct acaactcag gcggtttgc tcccaacaa 
      atggacccg aatggtcaa gaatccgaa aagtgttcc 
      agaaaggtc tctctggcc caaccgagg ctcttagtt 
      ttatcaaag gctcagatg acgtgatat ctgagtggt 
      tagatactc gcttcaaga aaattcaca aagagacgt 
      caaacatgg attttcttc aagaactct caaggttat 
      ttttagagc tgataggcg ttgccgctt ttgatataa 
      gatttcaaa gttttcacg atgatgaat tagacccgt 
      gctccaaaa gtacgaaat tattagaat cagcctttg 
      ttactaata gtacaattt taaagacgg ataatgggc 
      cccaattgt ggcgcaaat tcgaaacac cggcctaca 
      ggaggctga gaaaagcgc aggaattaa tggagagcg 
      ttgcgattg atttggtgg ctttgaagt tgtccacct 
      tcaaggaaa aaacatcaa atcctccca ctttgcttg 
      agcgatacc tcgcctctg cctctctgg acttcaagg 
      acattatag gcgtagtgt gcgattttt tgttggcag 
      gaatggaca ctaccacat actcctcaa gtttcctgc 
      tttaccact tagccacca acccttcaa ctcaagacg 
      cactttacg aggaggctt gccgtctcc tcccgaacc 
      ctgcggccc ctctaacta cagaaaaat acaaacaag 
      cagaatatg caaaatgtg ctgtgaaag aatctctaa 
      gattgcggc cgatttcga tcggtgtcg gccgccagt 
      tgactacag atgttgttt tttcagggt acaaagtgc 
      cttcaggga cggttgtag tgactttga accaagttt 
      tgagccgga tggagaaat attttccgg agcctgatt 
      catttaagc ctgaacgtt ggatgaaaa atgaccctt 
      cctacgtcc agacgcacc cgtatcttg tgatacctt 
      tcgggcatg ggcaaaggt cctgcattg cgagaaggt 
      ttgccgaac agaatatgg ttattttaa ttctaaagt 
      tggcgagaa aatacaaat tgagatgga acggatctg 
      aaatcgact caaaatccc tactgatca ataaaccag 
      atggaccaa ttttattgt cttttgagc caagatagt 
      atgtatgag tatatttgc aagtatttt tacatgtaa 
      ttattgcca ctaacgaaa tatattttt ataagtatt 
      atggtaatg catgaatga acctatcaa aaacgtaca 
      aattaaatt gcaaaaatt ggactaaac cagcgaggg 
      gtcctatag gtagtgtag agtgcggtg caatggtgg 
      caggataat taatcagaa aattatcac ggttttgta 
      ggtcgttcc gtatataaa aagtaaaca cttcatgta 
      taaggcatg aagtaatga taaatagca cacttgcat 
      ggacctaca attaattat ggtgttatt gtaaaatta 
      gttacaaat tacttacca ttgtgtagc accgttgaa 
      cgtagagat ggaactat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY