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Gene information
General information
TC013151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13155007..13156634
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013151
    iBeetle screen iB_08406

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013151 RA
      MFNQGNEEK KLDWMYKGP NSLVEREEY LLGRKVDKT 
LEQLNEEEK QKQQNIIVP PKNRVEHEC IPPSIRDFN 
KVVQAEQVD LSTKLQEDP LVAIKKREE ETRRQFLQN 
PVQLRKLQK ALKAQQAQK KKKKKKKKS KQSSDSDSD 
LDSKLTEKL LGKKKKNSK EERNLDTIL IHKFEALKD 
KLTESDLQD ILNGKITSS DESSEDEKP RNKSSKKRH 
DSEDSDDYR KHKKKEKKR DCSDDDAQR RKKSDRSHQ 
KNKTSKDSH SHRNRSRSP DIKKSNTKR RSSSSESES 
EEDVRNRHS KKYDSDDSG SSKHKKRNY GLVSSDGKK 
IPLSRKHDD VKISKPKVP EPPPPVQRH RAKKLTEEE 
KEKLRQEMM QNAVLRDKE RERNVRKYR EKEEKERKE 
QNFDPEFIH KQLMKTADT GSVESRIKS NLNNIQRSS 
KSMDQHFSR R
    CDS sequence
    • >TC013151 RA
      atgttcaac caaggcaat gaagagaaa aaactagac 
tggatgtac aaaggcccc aacagtttg gtggagcgt 
gaggagtac ttattgggg cgcaaagtt gacaaaaca 
ttagagcag ttaaatgaa gaggaaaaa cagaagcaa 
caaaatata attgtaccg ccgaaaaat cgtgtggaa 
catgagtgt attccaccg tcaattagg gacttcaat 
aaagttgta caagccgaa caggtagat ttgtctaca 
aaattgcaa gaagacccg ctggtggcg ataaaaaaa 
cgtgaagaa gagaccaga cggcaattt ttacaaaat 
cccgttcaa ttgagaaag ttacaaaaa gcgctgaaa 
gcccaacaa gcccaaaaa aagaagaaa aagaagaag 
aagaagtcg aagcaatct agcgactca gatagtgat 
ttggacagc aaattgacg gaaaagttg ttagggaag 
aagaagaaa aatagtaaa gaagaacgc aacttggat 
acgatttta attcacaag tttgaagca ctgaaggat 
aagttgact gaaagcgat ttacaagac attttgaat 
ggaaaaata acaagtagc gacgagagc agtgaagac 
gaaaagcca agaaacaaa tcatcgaag aaacgacac 
gatagcgaa gatagtgat gattacagg aaacacaag 
aagaaggag aaaaaaaga gactgttct gatgatgat 
gcacaaagg agaaaaaaa tccgacagg agccaccaa 
aaaaataaa acttcaaaa gattcccac tcacataga 
aacaggagt agaagtccc gatattaaa aaatctaat 
actaagaga agaagtagc agtagcgag tctgaaagt 
gaagaagac gtaagaaac agacactcc aagaaatac 
gacagtgac gattccggt tcttccaag cacaaaaag 
cgcaattac ggtcttgta agttcagat ggtaaaaaa 
attccgtta tcaagaaaa cacgatgat gtaaaaata 
tctaagcca aaagtacct gaacctcct cccccagtc 
caaagacac agagctaag aaactaacc gaagaagaa 
aaagaaaaa ttaagacaa gaaatgatg caaaatgca 
gttctaagg gacaaagaa cgcgaacgc aacgtgaga 
aaataccga gaaaaggag gaaaaagaa aggaaggag 
caaaacttt gatcctgag tttatccac aaacaacta 
atgaaaact gcggacact ggcagcgtt gaaagcaga 
attaagtca aacttgaat aatattcag agatcaagc 
aagtccatg gatcaacat ttctcccgg aggtag
    mRNA sequence
    • >TC013151 RA
      actacagcg ggtgtgttt ttaattatt tattttaca 
aataattaa tcaattatt gagtaaatg ctgttttaa 
aatattttg agtgattgt gaaagtagt gaagacgga 
acaatttag ttataattt attctttgg atattaata 
ttgccgtaa cctcaactt gggaacata atgttcaac 
caaggcaat gaagagaaa aaactagac tggatgtac 
aaaggcccc aacagtttg gtggagcgt gaggagtac 
ttattgggg cgcaaagtt gacaaaaca ttagagcag 
ttaaatgaa gaggaaaaa cagaagcaa caaaatata 
attgtaccg ccgaaaaat cgtgtggaa catgagtgt 
attccaccg tcaattagg gacttcaat aaagttgta 
caagccgaa caggtagat ttgtctaca aaattgcaa 
gaagacccg ctggtggcg ataaaaaaa cgtgaagaa 
gagaccaga cggcaattt ttacaaaat cccgttcaa 
ttgagaaag ttacaaaaa gcgctgaaa gcccaacaa 
gcccaaaaa aagaagaaa aagaagaag aagaagtcg 
aagcaatct agcgactca gatagtgat ttggacagc 
aaattgacg gaaaagttg ttagggaag aagaagaaa 
aatagtaaa gaagaacgc aacttggat acgatttta 
attcacaag tttgaagca ctgaaggat aagttgact 
gaaagcgat ttacaagac attttgaat ggaaaaata 
acaagtagc gacgagagc agtgaagac gaaaagcca 
agaaacaaa tcatcgaag aaacgacac gatagcgaa 
gatagtgat gattacagg aaacacaag aagaaggag 
aaaaaaaga gactgttct gatgatgat gcacaaagg 
agaaaaaaa tccgacagg agccaccaa aaaaataaa 
acttcaaaa gattcccac tcacataga aacaggagt 
agaagtccc gatattaaa aaatctaat actaagaga 
agaagtagc agtagcgag tctgaaagt gaagaagac 
gtaagaaac agacactcc aagaaatac gacagtgac 
gattccggt tcttccaag cacaaaaag cgcaattac 
ggtcttgta agttcagat ggtaaaaaa attccgtta 
tcaagaaaa cacgatgat gtaaaaata tctaagcca 
aaagtacct gaacctcct cccccagtc caaagacac 
agagctaag aaactaacc gaagaagaa aaagaaaaa 
ttaagacaa gaaatgatg caaaatgca gttctaagg 
gacaaagaa cgcgaacgc aacgtgaga aaataccga 
gaaaaggag gaaaaagaa aggaaggag caaaacttt 
gatcctgag tttatccac aaacaacta atgaaaact 
gcggacact ggcagcgtt gaaagcaga attaagtca 
aacttgaat aatattcag agatcaagc aagtccatg 
gatcaacat ttctcccgg aggtagtga aagttgtgt 
aagaataaa actttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY