Status
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Gene information
General information
TC033205
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030653,TC030762
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12668908..12672205
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033205
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08391

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature potentially posteriorshape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: It's same pic which is already used in above annotation.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033205 RA
      MQEAGNIDI ICEFLEIAI HNVLFARKL YPDSIFERK 
      RKYGVVVYR SVYPSLNEY IANCIKAIS YHLRNNQLK 
      NILLCFSSD QRVLEKYSF QILHLNAVS ESDPFLVKL 
      EQHLSTFFL KLHSSLDKL DNLPEDASF TIQLQVTEF 
      AHLEYKQDP LNENFLWVA APEYDNSSF PVIAPLHSV 
      NTGFVHFQI LVEKPSTVK GIFKRGDLE DSLSASDWS 
      SHNDHYKSR RRNRIVKLD SDPLLLTCE WDSCYQDFT 
      EFVPFVDHL KQHLHNYSL VGDGDCCWT GCTYVANSI 
      PDLNQHLCY HAYHTKLKC RGKSVLYRT NLPECTLTE 
      HFVIPVTPD GYLCEWEYC NERFETIFE LFEHVRVHI 
      YHNPKNHKQ GNITCCWKG CTNKTKFRT QCKLAEHMR 
      MHTKEKMVA CPTCGNLFS NVTKFCDHR RRQLPINLQ 
      SYLCSQCSK YFPSERLLR DHMRLHINH YKCSMCEMT 
      CPKPSILAK HIRYKHLSE RPFKCNYCD YECVSKHNL 
      DFHVKTHDP ESEYKCSDC GYECRSQFG LDRHYQKAH 
      GKDLTNVYE CHNCKKQFM RGGFLTKHL MKVHNYHWP 
      SGHSRFRYK EDADGIYRL QTVRYETLD VTEEIIKGK 
      TVQAPSEKE SLNFNVVKK TAKDSNSLH TFEVVFSEK 
      DETKSESEA AKEVLIQID DLDTKGQVV KSQTIHSIC 
      ADLEEISDC NSKEIVAIG DKTNEV
    • >TC033205 RB
      MQEADIICE FLEIAIHNV LFARKLYPD SIFERKRKY 
      GVVVYRSVY PSLNEYIAN CIKAISYHL RNNQLKNIL 
      LCFSSDQRV LEKYSFQIL HLNAVSESD PFLVKLEQH 
      LSTFFLKLH SSLDKLDNL PEDASFTIQ LQVTEFAHL 
      EYKQDPLNE NFLWVAAPE YDNSSFPVI APLHSVNTG 
      FVHFQILVE KPSTVKGIF KRGDLEDSL SASDWSSHN 
      DHYKSRRRN RIVKLDSDP LLLTCEWDS CYQDFTEFV 
      PFVDHLKQH LHNYSLVGD GDCCWTGCT YVANSIPDL 
      NQHLCYHAY HTKLKCRGK SVLYRTNLP ECTLTEHFV 
      IPVTPDGYL CEWEYCNER FETIFELFE HVRVHIYHN 
      PKNHKQGNI TCCWKGCTN KTKFRTQCK LAEHMRMHT 
      KEKMVACPT CGNLFSNVT KFCDHRRRQ LPINLQSYL 
      CSQCSKYFP SERLLRDHM RLHINHYKC SMCEMTCPK 
      PSILAKHIR YKHLSERPF KCNYCDYEC VSKHNLDFH 
      VKTHDPESE YKCSDCGYE CRSQFGLDR HYQKAHGKD 
      LTNVYECHN CKKQFMRGG FLTKHLMKV HNYHWPSGH 
      SRFRYKEDA DGIYRLQTV RYETLDVTE EIIKGKTVQ 
      APSEKESLN FNVVKKTAK DSNSLHTFE VVFSEKDET 
      KSESEAAKE VLIQIDDLD TKGQVVKSQ TIHSICADL 
      EEISDCNSK EIVAIGDKT NEV
    CDS sequence
    • >TC033205 RA
      atgcaagag gcgggtaat atcgacata atttgcgag 
      ttcctggaa atcgcaatt cacaacgtt ctgtttgct 
      cgcaagttg taccccgac tcaattttc gaacgcaag 
      cgtaaatac ggggtggtt gtttatcgt tctgtgtac 
      cctagctta aacgagtac attgccaat tgtattaag 
      gcaataagt taccatttg cggaacaac cagttgaaa 
      aatatcctt ctgtgcttt agttcagac cagagagta 
      ttagagaag tacagtttt caaattttg caccttaac 
      gccgtttcg gaaagcgat ccgttcctt gtgaaactg 
      gaacagcat ttaagtacg ttttttttg aaactacac 
      tcaagcctt gataagctt gataattta ccagaagac 
      gcctctttt accattcaa ttacaagtc acagagttt 
      gcgcatttg gagtacaag caagacccc cttaatgaa 
      aattttttg tgggtcgca gcgcccgaa tatgacaat 
      agttcgttt cccgtaata gcgcctttg catagtgtt 
      aacaccggt ttcgtccat tttcaaatt cttgtcgaa 
      aaaccgtca actgtgaaa ggaattttt aaaaggggc 
      gatttggag gattcttta tcggcttcc gattggagt 
      tcgcataat gatcattac aaatcaaga agaagaaat 
      cggattgtt aaactagat tctgaccct ttgcttctg 
      acgtgtgaa tgggattcg tgttatcag gattttacg 
      gaatttgtc ccctttgtc gaccatctg aagcaacat 
      ttgcacaat tacagttta gtcggagat ggggattgt 
      tgttggacg gggtgcact tacgtcgcc aactcgata 
      ccggaccta aaccagcac ttgtgctat cacgcctac 
      cataccaaa ttaaaatgt cgtggcaag agtgtctta 
      taccgcact aatttgccc gaatgcacc ctaaccgag 
      cactttgta attcccgta actcccgat gggtatctt 
      tgcgagtgg gagtactgt aacgagcga tttgagacg 
      atatttgaa ctctttgaa catgttcga gttcacatt 
      tatcacaat ccaaaaaac cacaaacag ggcaatatc 
      acttgttgc tggaaaggg tgcaccaat aaaacgaaa 
      tttcgcact cagtgtaag ttagctgag cacatgagg 
      atgcacaca aaggagaaa atggtggcg tgtccgact 
      tgtggtaac ttattttct aatgtaacg aagttttgt 
      gatcatcga cggaggcag ttgccgatt aatctacaa 
      agttatttg tgttcgcaa tgctcgaaa tatttccca 
      agtgaacgc ttattgagg gatcacatg agactgcat 
      attaaccat tacaagtgt tccatgtgc gaaatgacc 
      tgtcctaaa ccatcaatc cttgccaaa catatcaga 
      tacaaacat ttgagcgag agaccgttt aaatgtaac 
      tactgcgat tacgaatgc gtttcgaag cacaatttg 
      gatttccat gtgaaaacg cacgaccct gagagtgaa 
      tacaagtgc tctgattgt ggctatgaa tgccggtct 
      cagttcggt ttggaccga cattatcaa aaagcccac 
      ggaaaagac ttaactaac gtgtacgaa tgccacaac 
      tgtaaaaaa caatttatg cgtgggggt ttcttgact 
      aaacatttg atgaaagtc cacaattac cactggccg 
      tcgggtcac tcgaggttt cgctacaag gaagatgcg 
      gacgggatt tatcggctt caaacggtc cgatatgag 
      acacttgac gtgactgag gaaattatc aaagggaaa 
      acggttcaa gctccctca gagaaggaa tctttgaat 
      ttcaatgtg gtgaagaaa acggcgaaa gattctaat 
      tcgttgcat actttcgag gtggttttt tcggagaaa 
      gatgagaca aagagtgag agtgaagcc gcgaaggag 
      gtgttgatt caaatcgat gatcttgat acgaaagga 
      caagttgtc aagtcgcag acgatacat tcgatttgt 
      gcagatttg gaggagatt agtgattgc aatagcaaa 
      gaaattgta gctataggg gacaaaacg aatgaagtt 
      tag
    • >TC033205 RB
      atgcaagag gcggacata atttgcgag ttcctggaa 
      atcgcaatt cacaacgtt ctgtttgct cgcaagttg 
      taccccgac tcaattttc gaacgcaag cgtaaatac 
      ggggtggtt gtttatcgt tctgtgtac cctagctta 
      aacgagtac attgccaat tgtattaag gcaataagt 
      taccatttg cggaacaac cagttgaaa aatatcctt 
      ctgtgcttt agttcagac cagagagta ttagagaag 
      tacagtttt caaattttg caccttaac gccgtttcg 
      gaaagcgat ccgttcctt gtgaaactg gaacagcat 
      ttaagtacg ttttttttg aaactacac tcaagcctt 
      gataagctt gataattta ccagaagac gcctctttt 
      accattcaa ttacaagtc acagagttt gcgcatttg 
      gagtacaag caagacccc cttaatgaa aattttttg 
      tgggtcgca gcgcccgaa tatgacaat agttcgttt 
      cccgtaata gcgcctttg catagtgtt aacaccggt 
      ttcgtccat tttcaaatt cttgtcgaa aaaccgtca 
      actgtgaaa ggaattttt aaaaggggc gatttggag 
      gattcttta tcggcttcc gattggagt tcgcataat 
      gatcattac aaatcaaga agaagaaat cggattgtt 
      aaactagat tctgaccct ttgcttctg acgtgtgaa 
      tgggattcg tgttatcag gattttacg gaatttgtc 
      ccctttgtc gaccatctg aagcaacat ttgcacaat 
      tacagttta gtcggagat ggggattgt tgttggacg 
      gggtgcact tacgtcgcc aactcgata ccggaccta 
      aaccagcac ttgtgctat cacgcctac cataccaaa 
      ttaaaatgt cgtggcaag agtgtctta taccgcact 
      aatttgccc gaatgcacc ctaaccgag cactttgta 
      attcccgta actcccgat gggtatctt tgcgagtgg 
      gagtactgt aacgagcga tttgagacg atatttgaa 
      ctctttgaa catgttcga gttcacatt tatcacaat 
      ccaaaaaac cacaaacag ggcaatatc acttgttgc 
      tggaaaggg tgcaccaat aaaacgaaa tttcgcact 
      cagtgtaag ttagctgag cacatgagg atgcacaca 
      aaggagaaa atggtggcg tgtccgact tgtggtaac 
      ttattttct aatgtaacg aagttttgt gatcatcga 
      cggaggcag ttgccgatt aatctacaa agttatttg 
      tgttcgcaa tgctcgaaa tatttccca agtgaacgc 
      ttattgagg gatcacatg agactgcat attaaccat 
      tacaagtgt tccatgtgc gaaatgacc tgtcctaaa 
      ccatcaatc cttgccaaa catatcaga tacaaacat 
      ttgagcgag agaccgttt aaatgtaac tactgcgat 
      tacgaatgc gtttcgaag cacaatttg gatttccat 
      gtgaaaacg cacgaccct gagagtgaa tacaagtgc 
      tctgattgt ggctatgaa tgccggtct cagttcggt 
      ttggaccga cattatcaa aaagcccac ggaaaagac 
      ttaactaac gtgtacgaa tgccacaac tgtaaaaaa 
      caatttatg cgtgggggt ttcttgact aaacatttg 
      atgaaagtc cacaattac cactggccg tcgggtcac 
      tcgaggttt cgctacaag gaagatgcg gacgggatt 
      tatcggctt caaacggtc cgatatgag acacttgac 
      gtgactgag gaaattatc aaagggaaa acggttcaa 
      gctccctca gagaaggaa tctttgaat ttcaatgtg 
      gtgaagaaa acggcgaaa gattctaat tcgttgcat 
      actttcgag gtggttttt tcggagaaa gatgagaca 
      aagagtgag agtgaagcc gcgaaggag gtgttgatt 
      caaatcgat gatcttgat acgaaagga caagttgtc 
      aagtcgcag acgatacat tcgatttgt gcagatttg 
      gaggagatt agtgattgc aatagcaaa gaaattgta 
      gctataggg gacaaaacg aatgaagtt tag
    mRNA sequence
    • >TC033205 RA
      ttcatcaca tgaaacact cacaatatg ataaggaga 
      aattcgacg agagaatgg agaacattt cacagtgac 
      gtaagcgtt gagtcgtca cgtccgagc cacaatgac 
      gcacacgag acacttaac tcaaggaaa aaaccataa 
      aacgactga taattgttt agtttaaca cttattccg 
      ccttgtaaa ggaaataaa cgaacagca gctgcaacg 
      ataattatt ggtgtcaaa cgacttgtc aattagttg 
      gggtttttc gactcggat tggcaccta aaccagtcc 
      caccaatcg tccccgaaa attataaat atttttttc 
      gaaaaaaac aactcaaga agtgtccta attggcaaa 
      aactagtgt tatttgggt caattgtgc aaaattgcg 
      cattttagg attgttttt cacccgagc ttaaaaacg 
      cgccaagtt gagtcagct gactggtga cacaacctc 
      caatttgac ttgtttggt gatgtttca agtttttaa 
      tgcaagagg cgggtaata tcgacataa tttgcgagt 
      tcctggaaa tcgcaattc acaacgttc tgtttgctc 
      gcaagttgt accccgact caattttcg aacgcaagc 
      gtaaatacg gggtggttg tttatcgtt ctgtgtacc 
      ctagcttaa acgagtaca ttgccaatt gtattaagg 
      caataagtt accatttgc ggaacaacc agttgaaaa 
      atatccttc tgtgcttta gttcagacc agagagtat 
      tagagaagt acagttttc aaattttgc accttaacg 
      ccgtttcgg aaagcgatc cgttccttg tgaaactgg 
      aacagcatt taagtacgt tttttttga aactacact 
      caagccttg ataagcttg ataatttac cagaagacg 
      cctctttta ccattcaat tacaagtca cagagtttg 
      cgcatttgg agtacaagc aagaccccc ttaatgaaa 
      attttttgt gggtcgcag cgcccgaat atgacaata 
      gttcgtttc ccgtaatag cgcctttgc atagtgtta 
      acaccggtt tcgtccatt ttcaaattc ttgtcgaaa 
      aaccgtcaa ctgtgaaag gaattttta aaaggggcg 
      atttggagg attctttat cggcttccg attggagtt 
      cgcataatg atcattaca aatcaagaa gaagaaatc 
      ggattgtta aactagatt ctgaccctt tgcttctga 
      cgtgtgaat gggattcgt gttatcagg attttacgg 
      aatttgtcc cctttgtcg accatctga agcaacatt 
      tgcacaatt acagtttag tcggagatg gggattgtt 
      gttggacgg ggtgcactt acgtcgcca actcgatac 
      cggacctaa accagcact tgtgctatc acgcctacc 
      ataccaaat taaaatgtc gtggcaaga gtgtcttat 
      accgcacta atttgcccg aatgcaccc taaccgagc 
      actttgtaa ttcccgtaa ctcccgatg ggtatcttt 
      gcgagtggg agtactgta acgagcgat ttgagacga 
      tatttgaac tctttgaac atgttcgag ttcacattt 
      atcacaatc caaaaaacc acaaacagg gcaatatca 
      cttgttgct ggaaagggt gcaccaata aaacgaaat 
      ttcgcactc agtgtaagt tagctgagc acatgagga 
      tgcacacaa aggagaaaa tggtggcgt gtccgactt 
      gtggtaact tattttcta atgtaacga agttttgtg 
      atcatcgac ggaggcagt tgccgatta atctacaaa 
      gttatttgt gttcgcaat gctcgaaat atttcccaa 
      gtgaacgct tattgaggg atcacatga gactgcata 
      ttaaccatt acaagtgtt ccatgtgcg aaatgacct 
      gtcctaaac catcaatcc ttgccaaac atatcagat 
      acaaacatt tgagcgaga gaccgttta aatgtaact 
      actgcgatt acgaatgcg tttcgaagc acaatttgg 
      atttccatg tgaaaacgc acgaccctg agagtgaat 
      acaagtgct ctgattgtg gctatgaat gccggtctc 
      agttcggtt tggaccgac attatcaaa aagcccacg 
      gaaaagact taactaacg tgtacgaat gccacaact 
      gtaaaaaac aatttatgc gtgggggtt tcttgacta 
      aacatttga tgaaagtcc acaattacc actggccgt 
      cgggtcact cgaggtttc gctacaagg aagatgcgg 
      acgggattt atcggcttc aaacggtcc gatatgaga 
      cacttgacg tgactgagg aaattatca aagggaaaa 
      cggttcaag ctccctcag agaaggaat ctttgaatt 
      tcaatgtgg tgaagaaaa cggcgaaag attctaatt 
      cgttgcata ctttcgagg tggtttttt cggagaaag 
      atgagacaa agagtgaga gtgaagccg cgaaggagg 
      tgttgattc aaatcgatg atcttgata cgaaaggac 
      aagttgtca agtcgcaga cgatacatt cgatttgtg 
      cagatttgg aggagatta gtgattgca atagcaaag 
      aaattgtag ctatagggg acaaaacga atgaagttt 
      agttgggtg tgccaacgg tgttttatt tcattccca 
      attttttat ctagactat ttttatact caattactg 
      tgctccggc cacccgctg aagaagagt tcaagtcca 
      gttatgatg aagccgt
    • >TC033205 RB
      ttcatcaca tgaaacact cacaatatg ataaggaga 
      aattcgacg agagaatgg agaacattt cacagtgac 
      gtaagcgtt gagtcgtca cgtccgagc cacaatgac 
      gcacacgag acacttaac tcaaggaaa aaaccataa 
      aacgactga taattgttt agtttaaca cttattccg 
      ccttgtaaa ggaaataaa cgaacagca gctgcaacg 
      ataattatt ggtgtcaaa cgacttgtc aattagttg 
      gggtttttc gactcggat tggcaccta aaccagtcc 
      caccaatcg tccccgaaa attataaat atttttttc 
      gaaaaaaac aactcaaga agtgtccta attggcaaa 
      aactagtgt tatttgggt caattgtgc aaaattgcg 
      cattttagg attgttttt cacccgagc ttaaaaacg 
      cgccaagtt gagtcagct gactggtga cacaacctc 
      caatttgac ttgtttggt gatgtttca agtttttaa 
      tgcaagagg cggacataa tttgcgagt tcctggaaa 
      tcgcaattc acaacgttc tgtttgctc gcaagttgt 
      accccgact caattttcg aacgcaagc gtaaatacg 
      gggtggttg tttatcgtt ctgtgtacc ctagcttaa 
      acgagtaca ttgccaatt gtattaagg caataagtt 
      accatttgc ggaacaacc agttgaaaa atatccttc 
      tgtgcttta gttcagacc agagagtat tagagaagt 
      acagttttc aaattttgc accttaacg ccgtttcgg 
      aaagcgatc cgttccttg tgaaactgg aacagcatt 
      taagtacgt tttttttga aactacact caagccttg 
      ataagcttg ataatttac cagaagacg cctctttta 
      ccattcaat tacaagtca cagagtttg cgcatttgg 
      agtacaagc aagaccccc ttaatgaaa attttttgt 
      gggtcgcag cgcccgaat atgacaata gttcgtttc 
      ccgtaatag cgcctttgc atagtgtta acaccggtt 
      tcgtccatt ttcaaattc ttgtcgaaa aaccgtcaa 
      ctgtgaaag gaattttta aaaggggcg atttggagg 
      attctttat cggcttccg attggagtt cgcataatg 
      atcattaca aatcaagaa gaagaaatc ggattgtta 
      aactagatt ctgaccctt tgcttctga cgtgtgaat 
      gggattcgt gttatcagg attttacgg aatttgtcc 
      cctttgtcg accatctga agcaacatt tgcacaatt 
      acagtttag tcggagatg gggattgtt gttggacgg 
      ggtgcactt acgtcgcca actcgatac cggacctaa 
      accagcact tgtgctatc acgcctacc ataccaaat 
      taaaatgtc gtggcaaga gtgtcttat accgcacta 
      atttgcccg aatgcaccc taaccgagc actttgtaa 
      ttcccgtaa ctcccgatg ggtatcttt gcgagtggg 
      agtactgta acgagcgat ttgagacga tatttgaac 
      tctttgaac atgttcgag ttcacattt atcacaatc 
      caaaaaacc acaaacagg gcaatatca cttgttgct 
      ggaaagggt gcaccaata aaacgaaat ttcgcactc 
      agtgtaagt tagctgagc acatgagga tgcacacaa 
      aggagaaaa tggtggcgt gtccgactt gtggtaact 
      tattttcta atgtaacga agttttgtg atcatcgac 
      ggaggcagt tgccgatta atctacaaa gttatttgt 
      gttcgcaat gctcgaaat atttcccaa gtgaacgct 
      tattgaggg atcacatga gactgcata ttaaccatt 
      acaagtgtt ccatgtgcg aaatgacct gtcctaaac 
      catcaatcc ttgccaaac atatcagat acaaacatt 
      tgagcgaga gaccgttta aatgtaact actgcgatt 
      acgaatgcg tttcgaagc acaatttgg atttccatg 
      tgaaaacgc acgaccctg agagtgaat acaagtgct 
      ctgattgtg gctatgaat gccggtctc agttcggtt 
      tggaccgac attatcaaa aagcccacg gaaaagact 
      taactaacg tgtacgaat gccacaact gtaaaaaac 
      aatttatgc gtgggggtt tcttgacta aacatttga 
      tgaaagtcc acaattacc actggccgt cgggtcact 
      cgaggtttc gctacaagg aagatgcgg acgggattt 
      atcggcttc aaacggtcc gatatgaga cacttgacg 
      tgactgagg aaattatca aagggaaaa cggttcaag 
      ctccctcag agaaggaat ctttgaatt tcaatgtgg 
      tgaagaaaa cggcgaaag attctaatt cgttgcata 
      ctttcgagg tggtttttt cggagaaag atgagacaa 
      agagtgaga gtgaagccg cgaaggagg tgttgattc 
      aaatcgatg atcttgata cgaaaggac aagttgtca 
      agtcgcaga cgatacatt cgatttgtg cagatttgg 
      aggagatta gtgattgca atagcaaag aaattgtag 
      ctatagggg acaaaacga atgaagttt agttgggtg 
      tgccaacgg tgttttatt tcattccca attttttat 
      ctagactat ttttatact caattactg tgctccggc 
      cacccgctg aagaagagt tcaagtcca gttatgatg 
      aagccgt
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