Status
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Gene information
General information
TC033202
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014623
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12541647..12545755
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033202
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08387

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033202 RA
      MYPKPIQDL SGWTINHVA AGNTSIIVS 
      ADESVISWG STPTFGELG LGEITKSST 
      TPKEVTKLT GVKVLGLSM GFGHTLLIA 
      QNETPEEKT KLETFDVFE P
    CDS sequence
    • >TC033202 RA
      atgtatccg aaaccgata caggatttg agcggctgg 
      accatcaac catgtggct gcgggaaat acgtcaatt 
      attgtctcg gccgatgag tctgtcata tcatggggg 
      tccacaccc acctttgga gagttaggc cttggggaa 
      atcaccaaa tcttctacc actcctaaa gaggtcacg 
      aaattaacc ggagttaaa gtgctggga ttgtcaatg 
      ggattcgga cacaccttg ctaatagca cagaacgag 
      actcccgaa gagaaaacc aaacttgaa acctttgat 
      gttttcgaa ccctaa
    mRNA sequence
    • >TC033202 RA
      aatcaatta taatagcga gttggttta cctgtctgt 
      ctctatatc gtggctttt ccttaaacc actgctgct 
      ttatctgaa acgtctgaa agtaccgtt aaatcctgt 
      taaaccgtt aaaaagaaa attcagtgg aattttaca 
      gctgctaaa tacattgct ggttgctgg tggaccgag 
      tggtggacc aaataagta tgtggggtt tttcttgta 
      tggttattg gccattttg tatgattgc ttgtgttct 
      gtgtgttct aacggtatt tggtattgt aattgtaac 
      gttaaagtg tgagaaaga acaacaaaa actcccaag 
      aagcgaaag ctcggcgtc tccttaaac tatccctga 
      agcgttgtt tgtgtccat tgtttgcgt taataagtt 
      gtttacaaa tacggtgtg ttacgccag ttgattatt 
      tttgagttt tagaatgtc aacaaaacg gaagaaggc 
      gccagtcaa gagagggcc agctaaaag gaacaagag 
      gaaagaaga aagtgatgt ggattcgga tattagcga 
      cgccagcga tacggaaaa taccaccca acctgacga 
      agttctcat agctggaca tggggatgc tcttgacga 
      actcactca attacctca ggagttact taaaacttt 
      aatcaaacc cgctggaca actgcttat ttttggtct 
      catcaactg ggactacac gtccaacaa ggtccccaa 
      ggaagtcaa gattaaact gcaccccaa tctctacag 
      cccgcaccg gtttctcac agaccaaaa ggtgcgcct 
      ggctgtgag cggatgtgt ggccactca tagtgtact 
      tatcaccga ggagggcaa agcattgac attcggtcg 
      caataagtt cggtcagtt gggtctgga gcacaccga 
      aacgaaaga gattcctac cgtcgttcc ggcgttaga 
      gaacatgaa cgtaatcgg tgcagcgtg tggtcgcaa 
      tcatacgtt gtttctaac cgatacagg tactgtgta 
      tgcatgtgg tgacaaccg ctctggcca atgcggtgt 
      cggaaatct gcaacccaa tatcctcac tcccactcg 
      cattaacta ccgaggccc gccaatcgt gaaagtggg 
      ctgcggggg cggagtttt cgataattt tggacataa 
      aaggaggcc tgcactcgt tcggtttgc ccgaatacg 
      gccaattgg gccataaca ccgacggca aatatttca 
      aaaccaata ctaaactct gttttaatt tcgaaacta 
      gccccaaac gcattgtgc tgtacatcg agaaaagca 
      aagacggtc atgttgctc cagtagatg taactgaga 
      tcgttgatt tcagctgtg ggcagaatc acaccgtcg 
      cgattgata gcaaaaagc gggcgtttt cgtggggat 
      tcggcggat tcgggcgat taggacatg ccgagccga 
      gagacgaac tcgtcccac gtttgatca aatattttg 
      acacacagt ccaaagggc taaggtcta tacattgcg 
      gctccactt acagccttg cggtcaccg aattcggtg 
      ctttgttta tgttcggac agactaagc gtacaggcg 
      aagccaaca tgtatccga aaccgatac aggatttga 
      gcggctgga ccatcaacc atgtggctg cgggaaata 
      cgtcaatta ttgtctcgg ccgatgagt ctgtcatat 
      catgggggt ccacaccca cctttggag agttaggcc 
      ttggggaaa tcaccaaat cttctacca ctcctaaag 
      aggtcacga aattaaccg gagttaaag tgctgggat 
      tgtcaatgg gattcggac acaccttgc taatagcac 
      agaacgaga ctcccgaag agaaaacca aacttgaaa 
      cctttgatg ttttcgaac cctaatgtg gaaaaatac 
      gaaataata caggtatat cttattatg tactagaac 
      ttgtgtaac actactgcc cctttccat gttatttta 
      gccgttagc atttatact tcagtattt tttataata 
      atttattta gacattcta aattataat gtgtaatta 
      attgtacca ggttttgta tttgtttca agaaatcaa 
      taaacattt atatgaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY