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Gene information
General information
TC013193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12534842..12538865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013193
    iBeetle screen iB_08386

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013193 RA
      MKFPKNLFL VLFYTSWKF CDVCAYTPS HPLVYTKYG 
      SVIGSVEYS RNSRAYMSF KGIPFAKPP VGDLRFKAP 
      EPPEPWNFS INGTKDAPF CIQKNYFFS NPKVEGSED 
      CLYLNVYVP KTEGSQLLP VMVFIHWGG FFAGRGSSD 
      YIGPEYIMD KDVILVTFN YRLGVFGFL STLDDNAPG 
      NFGLKDQVM ALKFVHENI ECFGGDNNR VTIFGQSAG 
      SGSVNLHLI SPASRGLFQ QAISQSGAA LDLWARPLN 
      ALQPNVTAA LAAFTGCSA HIGSSKDIV DCLRKIEAT 
      KLAETADNF KYFSIEPLT PYSMVTEKQ TDANPNPFL 
      VQDPLESLK AGAFMKIPW MVGSVQDEG ILRAAPLIR 
      QPETLQTLN SNFEKLITQ MLFLQFSAG ANASSLLKN 
      MTDFYLGGK SLIDVNNPK SVQGFINLY GDRAFHYGI 
      YQTVILQLR KGHKPIWMY NFNYKGQYS YGDKFAATD 
      KNVNFTWGV SHCDDLLYL FKSPGLFAN LQKDNDILM 
      SKTMVSFWT NFAIYGNPD PHQNLNWNS LNFEKPEGV 
      KVADLNIMH ITGNHETGK IAFDVEKSQ ILDRIAFWA 
      KQSLLENFP DFG
    CDS sequence
    • >TC013193 RA
      atgaagttt cctaaaaac cttttctta gtgctgttc 
      tatacaagc tggaaattt tgtgatgtt tgtgcatac 
      actccttca catcctctt gtctacacc aagtatggc 
      tctgttatc ggatcagtg gaatattca agaaactcg 
      agagcttac atgtccttc aaaggaatc ccatttgcg 
      aaaccccca gtcggagac ttacgattt aaagcgcct 
      gaaccacca gaaccgtgg aatttttct ataaacgga 
      accaaagac gccccattt tgcatccag aaaaactac 
      ttctttagc aacccaaag gtagaagga agcgaagac 
      tgtttgtac ttaaatgta tacgtccca aaaactgaa 
      ggcagccag cttttgccc gtaatggtg ttcatccac 
      tggggcgga tttttcgcc ggccggggc tccagcgac 
      tacattggc cctgaatac atcatggat aaggacgtt 
      atcttggtc actttcaac taccgcttg ggcgtgttt 
      ggatttttg tccacactg gacgacaac gcgcctgga 
      aatttcggc ctgaaggac caagtcatg gctttgaag 
      ttcgttcac gaaaatatt gaatgcttc ggtggcgac 
      aacaacaga gtgacaata ttcggccaa agtgcaggc 
      agcggcagt gtcaacttg catttaata agtccagcc 
      tcgagaggc ttgtttcag caagccatc agtcaaagt 
      ggggctgct ttggacttg tgggcacga ccactgaac 
      gccctgcag ccgaatgtc accgctgca cttgctgca 
      tttaccgga tgttccgca cacataggc agcagtaaa 
      gatattgtt gattgttta aggaaaatt gaagccacc 
      aaattggcc gaaactgcc gacaatttc aaatatttt 
      tccatcgaa ccgctgact ccgtattca atggtcacg 
      gaaaaacaa acagacgcc aacccaaac cccttccta 
      gtccaagat cctctggag tcgctgaaa gctggcgcc 
      ttcatgaaa atcccctgg atggtgggt agtgtccaa 
      gatgaagga atcctcaga gctgctcct ttgatcaga 
      cagccggaa actttacaa acgcttaac tccaacttt 
      gaaaaactg ataacgcaa atgttgttc cttcaattc 
      agtgctggg gcgaacgca agctcactt ttaaaaaat 
      atgaccgac ttttacctc ggcgggaaa tccctcata 
      gacgtaaat aacccgaaa agtgttcaa ggatttatc 
      aatttatac ggcgacagg gcgtttcat tatggcatt 
      taccaaact gttattttg caattgaga aagggacac 
      aaaccgatt tggatgtac aatttcaat tacaaaggc 
      cagtacagt tatggtgat aagtttgcc gcgacggac 
      aaaaacgta aactttacg tggggtgtg agccactgt 
      gatgatttg ctctatcta ttcaagtcg cctgggctt 
      ttcgccaac ttgcagaaa gacaatgat attcttatg 
      agcaaaact atggtgtct ttttggaca aatttcgcg 
      atttatgga aatcccgat cctcatcaa aatctcaac 
      tggaatagc ttgaatttt gagaagcca gagggcgtc 
      aaagttgcg gatttgaat ataatgcat attactgga 
      aaccacgaa acgggcaag attgctttt gatgttgag 
      aaaagccaa attcttgac aggatcgca ttttgggcg 
      aaacaaagt cttcttgaa aatttccct gacttcggc 
      taa
    mRNA sequence
    • >TC013193 RA
      atcaatttc ataacagtg aagttggag caacccgtt 
      tgcctaggt atattttgt ataattggg tcattagta 
      atgagcttc aataataaa tttactgcc agtggcaat 
      gaaatgcgc tgctcacaa aaacctcgg aattatcag 
      aggttatcg tctcttttt atatttgtg ctaaaatgt 
      tttgtacct acttacctg tgtgctctt ttagtgtct 
      gaagtgaat tgtgtgttt cttcaattt tcagctaat 
      aaactatta atttacaca aagaacaaa acatgaagt 
      ttcctaaaa accttttct tagtgctgt tctatacaa 
      gctggaaat tttgtgatg tttgtgcat acactcctt 
      cacatcctc ttgtctaca ccaagtatg gctctgtta 
      tcggatcag tggaatatt caagaaact cgagagctt 
      acatgtcct tcaaaggaa tcccatttg cgaaacccc 
      cagtcggag acttacgat ttaaagcgc ctgaaccac 
      cagaaccgt ggaattttt ctataaacg gaaccaaag 
      acgccccat tttgcatcc agaaaaact acttcttta 
      gcaacccaa aggtagaag gaagcgaag actgtttgt 
      acttaaatg tatacgtcc caaaaactg aaggcagcc 
      agcttttgc ccgtaatgg tgttcatcc actggggcg 
      gatttttcg ccggccggg gctccagcg actacattg 
      gccctgaat acatcatgg ataaggacg ttatcttgg 
      tcactttca actaccgct tgggcgtgt ttggatttt 
      tgtccacac tggacgaca acgcgcctg gaaatttcg 
      gcctgaagg accaagtca tggctttga agttcgttc 
      acgaaaata ttgaatgct tcggtggcg acaacaaca 
      gagtgacaa tattcggcc aaagtgcag gcagcggca 
      gtgtcaact tgcatttaa taagtccag cctcgagag 
      gcttgtttc agcaagcca tcagtcaaa gtggggctg 
      ctttggact tgtgggcac gaccactga acgccctgc 
      agccgaatg tcaccgctg cacttgctg catttaccg 
      gatgttccg cacacatag gcagcagta aagatattg 
      ttgattgtt taaggaaaa ttgaagcca ccaaattgg 
      ccgaaactg ccgacaatt tcaaatatt tttccatcg 
      aaccgctga ctccgtatt caatggtca cggaaaaac 
      aaacagacg ccaacccaa accccttcc tagtccaag 
      atcctctgg agtcgctga aagctggcg ccttcatga 
      aaatcccct ggatggtgg gtagtgtcc aagatgaag 
      gaatcctca gagctgctc ctttgatca gacagccgg 
      aaactttac aaacgctta actccaact ttgaaaaac 
      tgataacgc aaatgttgt tccttcaat tcagtgctg 
      gggcgaacg caagctcac ttttaaaaa atatgaccg 
      acttttacc tcggcggga aatccctca tagacgtaa 
      ataacccga aaagtgttc aaggattta tcaatttat 
      acggcgaca gggcgtttc attatggca tttaccaaa 
      ctgttattt tgcaattga gaaagggac acaaaccga 
      tttggatgt acaatttca attacaaag gccagtaca 
      gttatggtg ataagtttg ccgcgacgg acaaaaacg 
      taaacttta cgtggggtg tgagccact gtgatgatt 
      tgctctatc tattcaagt cgcctgggc ttttcgcca 
      acttgcaga aagacaatg atattctta tgagcaaaa 
      ctatggtgt ctttttgga caaatttcg cgatttatg 
      gaaatcccg atcctcatc aaaatctca actggaata 
      gcttgaatt ttgagaagc cagagggcg tcaaagttg 
      cggatttga atataatgc atattactg gaaaccacg 
      aaacgggca agattgctt ttgatgttg agaaaagcc 
      aaattcttg acaggatcg cattttggg cgaaacaaa 
      gtcttcttg aaaatttcc ctgacttcg gctaattac 
      ttactactg tgtgtttta cttttttaa tttatattg 
      attacctta ttgatgtac aattccaat aaaattatt 
      cttttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY