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Gene information
General information
TC013626 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2938309..2942925
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013626
    iBeetle screen iB_08382

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013626 RA
      MESEEALNY YINEVKDTN NEIEIEEEQ VPNEAEQAA 
      DFLQKVGLS DLTKLYNQG KEITENVVS DSVRQKHLT 
      ERQAETVRS RIRTLNKTL HSRQPRRKQ RQDIRDVAW 
      NVETSSTGT RSRSATPDS LDSVGILND DLTSDEDQQ 
      SSLPPFPLE SSQPVFKAK VKWTSSEPL QNKKLSRDK 
      GDVAHLGSE NVILKGYQP LNEYSTLPR PQRERSGSD 
      PTTEVHLQP LKNRNSYEC KPLNLHNEF SRSQNNCSK 
      VYINGDENE ILSFEGLMR QNKVNQLTS ESFGLCDQG 
      IDIDEFTDS EYQYLKPLL YVELVAIFD QYKIVFLKR 
      KASGNVFGV NLSTLVMRD MQRPTDNLM VPKIFQSVV 
      NQLNTRCVC EDGILRLAG QKQKLEFLC NEIESKFFT 
      SRLEVETLL AQATVHELT GVLKKLLRD LPDPVFTME 
      LFDMFYKTS MIPNSEDKL KALNLLVLL LPIEHRNTY 
      KLLLQFLLN VVKHEKQNR MNLHNVAMI TAPSFFPAR 
      LLLPKDNNS KLIMKQLSQ EELAKHING AAVCCVIME 
      TMLRAGLKL WLIPSYLAR QAKEAQKRA QDKKDNSRD 
      RDKRIPCGK TKLVRSSTQ YEPGPINSP RIKKDLFI
    CDS sequence
    • >TC013626 RA
      atggaatca gaagaagcg ctcaactac tacattaac 
      gaagttaaa gacactaac aatgagata gaaatcgag 
      gaagagcaa gtccccaat gaagccgaa caagctgct 
      gactttctg caaaaggtc ggtttatcc gacttgacg 
      aagttgtac aaccaggga aaagaaata acagaaaat 
      gtagttagc gattccgtc aggcagaaa catttgact 
      gagagacaa gccgaaact gttaggtct agaatccgg 
      actttaaac aaaacactg cacagcaga cagccaagg 
      cggaaacaa cggcaagac attcgagac gtagcgtgg 
      aatgttgaa acatcgagt accggtacc aggtctaga 
      agtgctaca ccagattca ctcgactcg gtcggaatc 
      ctaaacgat gacctaact tcggacgaa gaccagcag 
      tcgtcttta cctcctttc ccacttgaa tctagtcaa 
      ccagtattt aaagcaaaa gttaaatgg acgtccagt 
      gaaccgctc cagaacaaa aaattgagc cgtgataag 
      ggagatgtg gcccatctt ggatctgaa aacgttatc 
      ttgaaaggt taccagccg ctgaacgag tactcgacc 
      ctcccgcga ccccaaaga gagcggagc gggagcgac 
      cccacaaca gaagtgcac ctccagccc ctcaaaaac 
      cggaacagt tacgaatgc aaaccgctc aacctccac 
      aacgaattc tcccgcagc cagaacaac tgcagcaaa 
      gtgtacata aacggcgac gagaacgaa atcctgagc 
      ttcgagggg ttgatgagg caaaacaaa gtcaatcaa 
      ctgacgtcc gaaagcttc ggtttgtgc gaccaaggc 
      atcgacatc gacgaattc accgacagc gaataccag 
      tacttaaag cccctactt tacgtagag ttagtcgct 
      atattcgac caatacaag atagttttt ctgaaacgg 
      aaggccagt gggaacgtc ttcggcgtt aatttgtcg 
      actctggta atgcgcgac atgcagcga ccgactgat 
      aacctaatg gtgcctaag atattccaa agcgtcgtc 
      aaccaactg aacactcgc tgcgtgtgc gaggacggg 
      attttgaga ctggccggc cagaagcag aagctcgag 
      tttttgtgc aacgagatc gagtcgaaa tttttcaca 
      agcaggctg gaggtggag acgctcctg gcgcaagca 
      acggtgcac gaactgacc ggggttttg aaaaagctc 
      ctccgggac ttgcccgac cccgtgttc accatggag 
      ctgttcgat atgttctac aaaacgagc atgattccg 
      aacagcgag gacaagttg aaagcttta aaccttctc 
      gtactcctc ctgccgatc gagcaccgc aacacctac 
      aaattgctt ctgcaattt cttttgaac gtggtcaag 
      catgaaaag cagaacagg atgaattta cacaacgtc 
      gcgatgatt acagccccg tcgtttttt cctgcaagg 
      ctgctgctt ccgaaagat aataacagt aaactgatt 
      atgaagcaa ctgtcgcag gaagagttg gccaaacat 
      ataaacggc gctgcggtt tgttgcgtc attatggag 
      accatgtta cgtgcagga ttgaaattg tggctaatt 
      ccgagctac ctggctcgg caagcgaag gaagcacaa 
      aaaagagcg caagacaaa aaggacaat agtagagat 
      agagacaaa agaattccg tgcggtaaa acaaagttg 
      gtcaggagc agcactcag tacgagcct ggccctatc 
      aactcgccg aggatcaaa aaggacttg tttatctag
    mRNA sequence
    • >TC013626 RA
      cgcctgaac taagccaac aggcggcgc tagcactac 
      ttaagtcga gtattttta catgttttt gtgcgtgtt 
      gtttttatt tttagtggg gagtgagtt cgtactgtt 
      tcatgtccg atattattt tagaatagg aagtggttg 
      aaggtgttt aatttttta attgttatt tgttggatt 
      tttacgacc gtcccttgt gatgtttgc tggcgtttg 
      gcaaagtca ataaataag ataaggagt ttcgttagt 
      gtttagtgt gtatacatt gctcatttt attatattg 
      ttataagtc acattttga atcgaagcc tcaagggac 
      catctcgtg atgtgtaaa gaaagaatt gcaatcatg 
      aagaacata taagtgtcc tagttatgg aatcagaag 
      aagcgctca actactaca ttaacgaag ttaaagaca 
      ctaacaatg agatagaaa tcgaggaag agcaagtcc 
      ccaatgaag ccgaacaag ctgctgact ttctgcaaa 
      aggtcggtt tatccgact tgacgaagt tgtacaacc 
      agggaaaag aaataacag aaaatgtag ttagcgatt 
      ccgtcaggc agaaacatt tgactgaga gacaagccg 
      aaactgtta ggtctagaa tccggactt taaacaaaa 
      cactgcaca gcagacagc caaggcgga aacaacggc 
      aagacattc gagacgtag cgtggaatg ttgaaacat 
      cgagtaccg gtaccaggt ctagaagtg ctacaccag 
      attcactcg actcggtcg gaatcctaa acgatgacc 
      taacttcgg acgaagacc agcagtcgt ctttacctc 
      ctttcccac ttgaatcta gtcaaccag tatttaaag 
      caaaagtta aatggacgt ccagtgaac cgctccaga 
      acaaaaaat tgagccgtg ataagggag atgtggccc 
      atcttggat ctgaaaacg ttatcttga aaggttacc 
      agccgctga acgagtact cgaccctcc cgcgacccc 
      aaagagagc ggagcggga gcgacccca caacagaag 
      tgcacctcc agcccctca aaaaccgga acagttacg 
      aatgcaaac cgctcaacc tccacaacg aattctccc 
      gcagccaga acaactgca gcaaagtgt acataaacg 
      gcgacgaga acgaaatcc tgagcttcg aggggttga 
      tgaggcaaa acaaagtca atcaactga cgtccgaaa 
      gcttcggtt tgtgcgacc aaggcatcg acatcgacg 
      aattcaccg acagcgaat accagtact taaagcccc 
      tactttacg tagagttag tcgctatat tcgaccaat 
      acaagatag tttttctga aacggaagg ccagtggga 
      acgtcttcg gcgttaatt tgtcgactc tggtaatgc 
      gcgacatgc agcgaccga ctgataacc taatggtgc 
      ctaagatat tccaaagcg tcgtcaacc aactgaaca 
      ctcgctgcg tgtgcgagg acgggattt tgagactgg 
      ccggccaga agcagaagc tcgagtttt tgtgcaacg 
      agatcgagt cgaaatttt tcacaagca ggctggagg 
      tggagacgc tcctggcgc aagcaacgg tgcacgaac 
      tgaccgggg ttttgaaaa agctcctcc gggacttgc 
      ccgaccccg tgttcacca tggagctgt tcgatatgt 
      tctacaaaa cgagcatga ttccgaaca gcgaggaca 
      agttgaaag ctttaaacc ttctcgtac tcctcctgc 
      cgatcgagc accgcaaca cctacaaat tgcttctgc 
      aatttcttt tgaacgtgg tcaagcatg aaaagcaga 
      acaggatga atttacaca acgtcgcga tgattacag 
      ccccgtcgt tttttcctg caaggctgc tgcttccga 
      aagataata acagtaaac tgattatga agcaactgt 
      cgcaggaag agttggcca aacatataa acggcgctg 
      cggtttgtt gcgtcatta tggagacca tgttacgtg 
      caggattga aattgtggc taattccga gctacctgg 
      ctcggcaag cgaaggaag cacaaaaaa gagcgcaag 
      acaaaaagg acaatagta gagatagag acaaaagaa 
      ttccgtgcg gtaaaacaa agttggtca ggagcagca 
      ctcagtacg agcctggcc ctatcaact cgccgagga 
      tcaaaaagg acttgttta tctagaccc aagaatcga 
      attgtataa catattcct caataacgt accttatca 
      accccttaa tatctagta atatattta atacttggt 
      ttacaattc tctgtgcca aaataaatt ttaattttt 
      aat
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