| General information | |||
| TC014609 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 12350384..12356625 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014609 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08381 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014609 RA
MASTLGKIA LGGLTTTLG AGLASYLFL TDDKDNRWG AVYAQHSPP KAKRNLPSR EEQIKSLQN DSFDVLIVG GGATGAGCA LDAVTRGLK TALVEADDF ASGTSSRST KLIHGGVRY LQKAIMQLD IEQYRMVKE ALHERASML QSAPHLAHP LPIMLPVYT WWQVPYYWV GIKMYDVVA GRKTLKSSY LLSKKNALE LFPMLRGDK LCGAIVYYD GQQDDARMN LAIALTATR HGATVANHV SVTGLLKEK DSSGKEIVC GVSVKDEIT GNKWNVPAK CVINATGPF TDSIRKMDN PEVKTICSP SSGVHITLP GYYSPEQMG LLDPSTSDG RVIFFLPWQ KQTIAGTTD LPCDVTHHP KPTEDEILF ILEEVKNYL NPDVEVRRG DVLSAWSGI RPLVSDPNK PDTQSLARN HIVHVSDSK LVTIAGGKW TTYRAMAQE TIDAAIAAC CLKPVHKEC QTDGLLLEG AHGWTPTMY IRLVQDFGL ECEVAQHLS KSYGDRAFT VAKMAALTG KRWPIIGKK IHPEFPYID AEVRYGVRE YAVTAVDMI ARRLRLAFL NVQAAQEAL PEIITIMAE ELGWSDEEK QKQHKDATI FLQNEMGQN VNRASRDKI PINLSKEEI QLYIKRFQI IDKDRKGYV SINDIRRSL KSLGMKLTD DEIHHLLSD INVTYNGQL ELADYLQMM SAIKSGHVT YSRFARMAE LEEQKHEKE MLKKKISVE RSGGGV
- >TC014609 RB
MASTLGKIA LGGLTTTLG AGLASYLFL TDDKDNRWG AVYAQHSPP KAKRNLPSR EEQIKSLQN DSFDVLIVG GGATGAGCA LDAVTRGLK TALVEADDF ASGTSSRST KLIHGGVRY LQKAIMQLD IEQYRMVKE ALHERASML QSAPHLAHP LPIMLPVYT WWQVPYYWV GIKMYDVVA GRKTLKSSY LLSKKNALE LFPMLRGDK LCGAIVYYD GQQDDARMN LAIALTATR HGATVANHV SVTGLLKEK DSSGKEIVC GVSVKDEIT GNKWNVPAK CVINATGPF TDSIRKMDN PEVKTICSP SSGVHITLP GYYSPEQMG LLDPSTSDG RVIFFLPWQ KQTIAGTTD LPCDVTHHP KPTEDEILF ILEEVKNYL NPDVEVRRG DVLSAWSGI RPLVSDPNK PDTQSLARN HIVHVSDSK LVTIAGGKW TTYRAMAQE TIDAAIAAC CLKPVHKEC QTDGLLLEG AHGWTPTMY IRLVQDFGL ECEVAQHLS KSYGDRAFT VAKMAALTG KRWPIIGKK IHPEFPYID AEVRYGVRE YAVTAVDMI ARRLRLAFL NVQAAQEAL PEIITIMAE ELGWSDEEK QKQHKDATI FLQNEMGQN VNRASRDKI PINLSKEEI QLYIKRFQI IDKDRKGYV SINDIRRSL KSLGMKLTD DEIHHLLSD INVTYNGQL ELADYLQRD DGKEVPGEE LHEILREID TNMNGQVEL DEYLQMMSA IKSGHVTYS RFARMAELE EQKHEKEML KKKISVERS GGGV
- >TC014609 RA
atggcctca acccttgga aaaatcgca ctgggtggc ctcaccacc acgctgggt gccggccta gcctcttac ctcttcctc acagacgac aaagacaat cgatggggc gccgtttac gcccaacac agcccccct aaggccaaa cgcaacctc ccgagccgc gaggaacaa ataaaatcg ttgcagaac gactccttc gatgtgctt atcgtcgga ggtggggcc acaggggct ggatgtgcg ctagacgcc gtcaccagg gggctcaag acggctctc gtggaggcc gacgatttc gcaagtggg acttctagt cgcagcacg aagctgatc catggaggg gtgcgctac ctccagaag gcgatcatg cagctggat atcgagcag tataggatg gtcaaagag gcgctgcat gagagggcg tctatgtta cagtcagcc ccacacttg gcgcatcct ctgcccatc atgctcccc gtttatacg tggtggcaa gtgccatat tactgggtt ggcataaaa atgtacgac gttgtcgct ggtcggaag accctcaaa tcatcgtat ctcctctca aagaaaaac gccttggag ttgtttcca atgcttcga ggagacaaa ctttgtggt gctatagtt tattacgac ggccaacaa gacgacgcc cgaatgaat ctcgccatt gctttaaca gccacccgt cacggcgcc actgtggca aaccacgtt tctgtgacc ggcctcctc aaagaaaaa gattcttcc ggcaaagaa atcgtgtgt ggcgtcagt gtcaaggac gaaatcacc ggaaacaaa tggaatgtc ccggcaaaa tgtgtgata aacgccact ggacccttc acggactcc atccggaag atggacaat ccggaagtg aaaacgatt tgttcgccc agcagtggc gtccatatc actcttcct gggtattac agcccggag cagatgggt ttgcttgac cccagtacc agtgacggt cgcgtgatt ttcttcctg ccgtggcag aagcagacc atcgctggt acgacggac ctcccatgc gacgtcacc caccatccc aagcccacc gaggacgag attttattt attttagaa gaagttaag aattatttg aatccggat gtggaagtg aggcgaggt gatgtcttg tcggcctgg agcggtata aggccactc gtttccgat ccgaataag ccggacacg cagtcgctt gcccgtaac cacattgtt catgttagt gatagtaaa ttggtaacg atagctggg ggtaaatgg accacgtat cgtgccatg gctcaggag actattgat gcggcgatt gccgcctgt tgtctcaag ccggtgcat aaagagtgt cagactgac gggttgttg ctggagggg gcgcacgga tggaccccc accatgtac attcggtta gtgcaggat ttcggcctg gagtgcgag gtggcgcaa catttgtcc aagtcctac ggcgacagg gcgttcacg gtggccaag atggcagcg ctgacaggg aagcggtgg cccatcatc ggcaaaaaa atccacccc gagttcccg tacatcgac gccgaggtc agatacggc gtccgagag tacgcagtg actgccgtc gacatgatc gcacgaagg cttcgtttg gctttcctc aacgtgcaa gccgctcaa gaagccctc cctgagatt atcacaatc atggccgag gagttaggc tggtctgac gaggagaag caaaagcag cacaaagac gccacaatc ttcctccag aacgagatg ggccagaac gtgaaccgc gccagcaga gacaaaatc ccgatcaat ttgagcaaa gaagagata cagttgtac attaagcgg ttccaaatt atcgacaag gaccgcaag ggatacgtc tcgatcaat gatataagg aggagtctc aagtctcta ggtatgaaa ctaaccgac gacgaaatc caccacctc ttatccgat attaacgta acttacaac ggccaattg gaactggct gattacttg cagatgatg tctgccatc aagtcaggc catgtgacg tattcgaga ttcgcccga atggctgaa ctcgaagaa caaaaacac gagaaagaa atgctcaag aagaagatt tcggtcgag cgcagcggt ggcggcgtg taa
- >TC014609 RB
atggcctca acccttgga aaaatcgca ctgggtggc ctcaccacc acgctgggt gccggccta gcctcttac ctcttcctc acagacgac aaagacaat cgatggggc gccgtttac gcccaacac agcccccct aaggccaaa cgcaacctc ccgagccgc gaggaacaa ataaaatcg ttgcagaac gactccttc gatgtgctt atcgtcgga ggtggggcc acaggggct ggatgtgcg ctagacgcc gtcaccagg gggctcaag acggctctc gtggaggcc gacgatttc gcaagtggg acttctagt cgcagcacg aagctgatc catggaggg gtgcgctac ctccagaag gcgatcatg cagctggat atcgagcag tataggatg gtcaaagag gcgctgcat gagagggcg tctatgtta cagtcagcc ccacacttg gcgcatcct ctgcccatc atgctcccc gtttatacg tggtggcaa gtgccatat tactgggtt ggcataaaa atgtacgac gttgtcgct ggtcggaag accctcaaa tcatcgtat ctcctctca aagaaaaac gccttggag ttgtttcca atgcttcga ggagacaaa ctttgtggt gctatagtt tattacgac ggccaacaa gacgacgcc cgaatgaat ctcgccatt gctttaaca gccacccgt cacggcgcc actgtggca aaccacgtt tctgtgacc ggcctcctc aaagaaaaa gattcttcc ggcaaagaa atcgtgtgt ggcgtcagt gtcaaggac gaaatcacc ggaaacaaa tggaatgtc ccggcaaaa tgtgtgata aacgccact ggacccttc acggactcc atccggaag atggacaat ccggaagtg aaaacgatt tgttcgccc agcagtggc gtccatatc actcttcct gggtattac agcccggag cagatgggt ttgcttgac cccagtacc agtgacggt cgcgtgatt ttcttcctg ccgtggcag aagcagacc atcgctggt acgacggac ctcccatgc gacgtcacc caccatccc aagcccacc gaggacgag attttattt attttagaa gaagttaag aattatttg aatccggat gtggaagtg aggcgaggt gatgtcttg tcggcctgg agcggtata aggccactc gtttccgat ccgaataag ccggacacg cagtcgctt gcccgtaac cacattgtt catgttagt gatagtaaa ttggtaacg atagctggg ggtaaatgg accacgtat cgtgccatg gctcaggag actattgat gcggcgatt gccgcctgt tgtctcaag ccggtgcat aaagagtgt cagactgac gggttgttg ctggagggg gcgcacgga tggaccccc accatgtac attcggtta gtgcaggat ttcggcctg gagtgcgag gtggcgcaa catttgtcc aagtcctac ggcgacagg gcgttcacg gtggccaag atggcagcg ctgacaggg aagcggtgg cccatcatc ggcaaaaaa atccacccc gagttcccg tacatcgac gccgaggtc agatacggc gtccgagag tacgcagtg actgccgtc gacatgatc gcacgaagg cttcgtttg gctttcctc aacgtgcaa gccgctcaa gaagccctc cctgagatt atcacaatc atggccgag gagttaggc tggtctgac gaggagaag caaaagcag cacaaagac gccacaatc ttcctccag aacgagatg ggccagaac gtgaaccgc gccagcaga gacaaaatc ccgatcaat ttgagcaaa gaagagata cagttgtac attaagcgg ttccaaatt atcgacaag gaccgcaag ggatacgtc tcgatcaat gatataagg aggagtctc aagtctcta ggtatgaaa ctaaccgac gacgaaatc caccacctc ttatccgat attaacgta acttacaac ggccaattg gaactggct gattacttg cagcgggat gatggaaag gaggttcct ggtgaggag ctccacgaa atccttcgg gagattgat acgaatatg aatggacaa gttgaactt gacgagtat ttgcagatg atgtctgcc atcaagtca ggccatgtg acgtattcg agattcgcc cgaatggct gaactcgaa gaacaaaaa cacgagaaa gaaatgctc aagaagaag atttcggtc gagcgcagc ggtggcggc gtgtaa
- >TC014609 RA
tcacagttt gtaaattgg aaaaatggc ctcaaccct tggaaaaat cgcactggg tggcctcac caccacgct gggtgccgg cctagcctc ttacctctt cctcacaga cgacaaaga caatcgatg gggcgccgt ttacgccca acacagccc ccctaaggc caaacgcaa cctcccgag ccgcgagga acaaataaa atcgttgca gaacgactc cttcgatgt gcttatcgt cggaggtgg ggccacagg ggctggatg tgcgctaga cgccgtcac cagggggct caagacggc tctcgtgga ggccgacga tttcgcaag tgggacttc tagtcgcag cacgaagct gatccatgg aggggtgcg ctacctcca gaaggcgat catgcagct ggatatcga gcagtatag gatggtcaa agaggcgct gcatgagag ggcgtctat gttacagtc agccccaca cttggcgca tcctctgcc catcatgct ccccgttta tacgtggtg gcaagtgcc atattactg ggttggcat aaaaatgta cgacgttgt cgctggtcg gaagaccct caaatcatc gtatctcct ctcaaagaa aaacgcctt ggagttgtt tccaatgct tcgaggaga caaactttg tggtgctat agtttatta cgacggcca acaagacga cgcccgaat gaatctcgc cattgcttt aacagccac ccgtcacgg cgccactgt ggcaaacca cgtttctgt gaccggcct cctcaaaga aaaagattc ttccggcaa agaaatcgt gtgtggcgt cagtgtcaa ggacgaaat caccggaaa caaatggaa tgtcccggc aaaatgtgt gataaacgc cactggacc cttcacgga ctccatccg gaagatgga caatccgga agtgaaaac gatttgttc gcccagcag tggcgtcca tatcactct tcctgggta ttacagccc ggagcagat gggtttgct tgaccccag taccagtga cggtcgcgt gattttctt cctgccgtg gcagaagca gaccatcgc tggtacgac ggacctccc atgcgacgt cacccacca tcccaagcc caccgagga cgagatttt atttatttt agaagaagt taagaatta tttgaatcc ggatgtgga agtgaggcg aggtgatgt cttgtcggc ctggagcgg tataaggcc actcgtttc cgatccgaa taagccgga cacgcagtc gcttgcccg taaccacat tgttcatgt tagtgatag taaattggt aacgatagc tgggggtaa atggaccac gtatcgtgc catggctca ggagactat tgatgcggc gattgccgc ctgttgtct caagccggt gcataaaga gtgtcagac tgacgggtt gttgctgga gggggcgca cggatggac ccccaccat gtacattcg gttagtgca ggatttcgg cctggagtg cgaggtggc gcaacattt gtccaagtc ctacggcga cagggcgtt cacggtggc caagatggc agcgctgac agggaagcg gtggcccat catcggcaa aaaaatcca ccccgagtt cccgtacat cgacgccga ggtcagata cggcgtccg agagtacgc agtgactgc cgtcgacat gatcgcacg aaggcttcg tttggcttt cctcaacgt gcaagccgc tcaagaagc cctccctga gattatcac aatcatggc cgaggagtt aggctggtc tgacgagga gaagcaaaa gcagcacaa agacgccac aatcttcct ccagaacga gatgggcca gaacgtgaa ccgcgccag cagagacaa aatcccgat caatttgag caaagaaga gatacagtt gtacattaa gcggttcca aattatcga caaggaccg caagggata cgtctcgat caatgatat aaggaggag tctcaagtc tctaggtat gaaactaac cgacgacga aatccacca cctcttatc cgatattaa cgtaactta caacggcca attggaact ggctgatta cttgcagat gatgtctgc catcaagtc aggccatgt gacgtattc gagattcgc ccgaatggc tgaactcga agaacaaaa acacgagaa agaaatgct caagaagaa gatttcggt cgagcgcag cggtggcgg cgtgtaaat taaatttgt gaataaaaa ttgtgtcaa tttgctact caggtgatt ttgtcgtat cgtttgagt taatttatt ttgttgtag ctctcgttt taaataaaa taaaaagta ccacaaaca
- >TC014609 RB
tcacagttt gtaaattgg aaaaatggc ctcaaccct tggaaaaat cgcactggg tggcctcac caccacgct gggtgccgg cctagcctc ttacctctt cctcacaga cgacaaaga caatcgatg gggcgccgt ttacgccca acacagccc ccctaaggc caaacgcaa cctcccgag ccgcgagga acaaataaa atcgttgca gaacgactc cttcgatgt gcttatcgt cggaggtgg ggccacagg ggctggatg tgcgctaga cgccgtcac cagggggct caagacggc tctcgtgga ggccgacga tttcgcaag tgggacttc tagtcgcag cacgaagct gatccatgg aggggtgcg ctacctcca gaaggcgat catgcagct ggatatcga gcagtatag gatggtcaa agaggcgct gcatgagag ggcgtctat gttacagtc agccccaca cttggcgca tcctctgcc catcatgct ccccgttta tacgtggtg gcaagtgcc atattactg ggttggcat aaaaatgta cgacgttgt cgctggtcg gaagaccct caaatcatc gtatctcct ctcaaagaa aaacgcctt ggagttgtt tccaatgct tcgaggaga caaactttg tggtgctat agtttatta cgacggcca acaagacga cgcccgaat gaatctcgc cattgcttt aacagccac ccgtcacgg cgccactgt ggcaaacca cgtttctgt gaccggcct cctcaaaga aaaagattc ttccggcaa agaaatcgt gtgtggcgt cagtgtcaa ggacgaaat caccggaaa caaatggaa tgtcccggc aaaatgtgt gataaacgc cactggacc cttcacgga ctccatccg gaagatgga caatccgga agtgaaaac gatttgttc gcccagcag tggcgtcca tatcactct tcctgggta ttacagccc ggagcagat gggtttgct tgaccccag taccagtga cggtcgcgt gattttctt cctgccgtg gcagaagca gaccatcgc tggtacgac ggacctccc atgcgacgt cacccacca tcccaagcc caccgagga cgagatttt atttatttt agaagaagt taagaatta tttgaatcc ggatgtgga agtgaggcg aggtgatgt cttgtcggc ctggagcgg tataaggcc actcgtttc cgatccgaa taagccgga cacgcagtc gcttgcccg taaccacat tgttcatgt tagtgatag taaattggt aacgatagc tgggggtaa atggaccac gtatcgtgc catggctca ggagactat tgatgcggc gattgccgc ctgttgtct caagccggt gcataaaga gtgtcagac tgacgggtt gttgctgga gggggcgca cggatggac ccccaccat gtacattcg gttagtgca ggatttcgg cctggagtg cgaggtggc gcaacattt gtccaagtc ctacggcga cagggcgtt cacggtggc caagatggc agcgctgac agggaagcg gtggcccat catcggcaa aaaaatcca ccccgagtt cccgtacat cgacgccga ggtcagata cggcgtccg agagtacgc agtgactgc cgtcgacat gatcgcacg aaggcttcg tttggcttt cctcaacgt gcaagccgc tcaagaagc cctccctga gattatcac aatcatggc cgaggagtt aggctggtc tgacgagga gaagcaaaa gcagcacaa agacgccac aatcttcct ccagaacga gatgggcca gaacgtgaa ccgcgccag cagagacaa aatcccgat caatttgag caaagaaga gatacagtt gtacattaa gcggttcca aattatcga caaggaccg caagggata cgtctcgat caatgatat aaggaggag tctcaagtc tctaggtat gaaactaac cgacgacga aatccacca cctcttatc cgatattaa cgtaactta caacggcca attggaact ggctgatta cttgcagcg ggatgatgg aaaggaggt tcctggtga ggagctcca cgaaatcct tcgggagat tgatacgaa tatgaatgg acaagttga acttgacga gtatttgca gatgatgtc tgccatcaa gtcaggcca tgtgacgta ttcgagatt cgcccgaat ggctgaact cgaagaaca aaaacacga gaaagaaat gctcaagaa gaagatttc ggtcgagcg cagcggtgg cggcgtgta aattaaatt tgtgaataa aaattgtgt caatttgct actcaggtg attttgtcg tatcgtttg agttaattt attttgttg tagctctcg ttttaaata aaataaaaa gtaccacaa aca
