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Gene information
General information
TC014609 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12350384..12356625
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014609
    iBeetle screen iB_08381

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014609 RA
      MASTLGKIA LGGLTTTLG AGLASYLFL TDDKDNRWG 
      AVYAQHSPP KAKRNLPSR EEQIKSLQN DSFDVLIVG 
      GGATGAGCA LDAVTRGLK TALVEADDF ASGTSSRST 
      KLIHGGVRY LQKAIMQLD IEQYRMVKE ALHERASML 
      QSAPHLAHP LPIMLPVYT WWQVPYYWV GIKMYDVVA 
      GRKTLKSSY LLSKKNALE LFPMLRGDK LCGAIVYYD 
      GQQDDARMN LAIALTATR HGATVANHV SVTGLLKEK 
      DSSGKEIVC GVSVKDEIT GNKWNVPAK CVINATGPF 
      TDSIRKMDN PEVKTICSP SSGVHITLP GYYSPEQMG 
      LLDPSTSDG RVIFFLPWQ KQTIAGTTD LPCDVTHHP 
      KPTEDEILF ILEEVKNYL NPDVEVRRG DVLSAWSGI 
      RPLVSDPNK PDTQSLARN HIVHVSDSK LVTIAGGKW 
      TTYRAMAQE TIDAAIAAC CLKPVHKEC QTDGLLLEG 
      AHGWTPTMY IRLVQDFGL ECEVAQHLS KSYGDRAFT 
      VAKMAALTG KRWPIIGKK IHPEFPYID AEVRYGVRE 
      YAVTAVDMI ARRLRLAFL NVQAAQEAL PEIITIMAE 
      ELGWSDEEK QKQHKDATI FLQNEMGQN VNRASRDKI 
      PINLSKEEI QLYIKRFQI IDKDRKGYV SINDIRRSL 
      KSLGMKLTD DEIHHLLSD INVTYNGQL ELADYLQMM 
      SAIKSGHVT YSRFARMAE LEEQKHEKE MLKKKISVE 
      RSGGGV
    • >TC014609 RB
      MASTLGKIA LGGLTTTLG AGLASYLFL TDDKDNRWG 
      AVYAQHSPP KAKRNLPSR EEQIKSLQN DSFDVLIVG 
      GGATGAGCA LDAVTRGLK TALVEADDF ASGTSSRST 
      KLIHGGVRY LQKAIMQLD IEQYRMVKE ALHERASML 
      QSAPHLAHP LPIMLPVYT WWQVPYYWV GIKMYDVVA 
      GRKTLKSSY LLSKKNALE LFPMLRGDK LCGAIVYYD 
      GQQDDARMN LAIALTATR HGATVANHV SVTGLLKEK 
      DSSGKEIVC GVSVKDEIT GNKWNVPAK CVINATGPF 
      TDSIRKMDN PEVKTICSP SSGVHITLP GYYSPEQMG 
      LLDPSTSDG RVIFFLPWQ KQTIAGTTD LPCDVTHHP 
      KPTEDEILF ILEEVKNYL NPDVEVRRG DVLSAWSGI 
      RPLVSDPNK PDTQSLARN HIVHVSDSK LVTIAGGKW 
      TTYRAMAQE TIDAAIAAC CLKPVHKEC QTDGLLLEG 
      AHGWTPTMY IRLVQDFGL ECEVAQHLS KSYGDRAFT 
      VAKMAALTG KRWPIIGKK IHPEFPYID AEVRYGVRE 
      YAVTAVDMI ARRLRLAFL NVQAAQEAL PEIITIMAE 
      ELGWSDEEK QKQHKDATI FLQNEMGQN VNRASRDKI 
      PINLSKEEI QLYIKRFQI IDKDRKGYV SINDIRRSL 
      KSLGMKLTD DEIHHLLSD INVTYNGQL ELADYLQRD 
      DGKEVPGEE LHEILREID TNMNGQVEL DEYLQMMSA 
      IKSGHVTYS RFARMAELE EQKHEKEML KKKISVERS 
      GGGV
    CDS sequence
    • >TC014609 RA
      atggcctca acccttgga aaaatcgca ctgggtggc 
      ctcaccacc acgctgggt gccggccta gcctcttac 
      ctcttcctc acagacgac aaagacaat cgatggggc 
      gccgtttac gcccaacac agcccccct aaggccaaa 
      cgcaacctc ccgagccgc gaggaacaa ataaaatcg 
      ttgcagaac gactccttc gatgtgctt atcgtcgga 
      ggtggggcc acaggggct ggatgtgcg ctagacgcc 
      gtcaccagg gggctcaag acggctctc gtggaggcc 
      gacgatttc gcaagtggg acttctagt cgcagcacg 
      aagctgatc catggaggg gtgcgctac ctccagaag 
      gcgatcatg cagctggat atcgagcag tataggatg 
      gtcaaagag gcgctgcat gagagggcg tctatgtta 
      cagtcagcc ccacacttg gcgcatcct ctgcccatc 
      atgctcccc gtttatacg tggtggcaa gtgccatat 
      tactgggtt ggcataaaa atgtacgac gttgtcgct 
      ggtcggaag accctcaaa tcatcgtat ctcctctca 
      aagaaaaac gccttggag ttgtttcca atgcttcga 
      ggagacaaa ctttgtggt gctatagtt tattacgac 
      ggccaacaa gacgacgcc cgaatgaat ctcgccatt 
      gctttaaca gccacccgt cacggcgcc actgtggca 
      aaccacgtt tctgtgacc ggcctcctc aaagaaaaa 
      gattcttcc ggcaaagaa atcgtgtgt ggcgtcagt 
      gtcaaggac gaaatcacc ggaaacaaa tggaatgtc 
      ccggcaaaa tgtgtgata aacgccact ggacccttc 
      acggactcc atccggaag atggacaat ccggaagtg 
      aaaacgatt tgttcgccc agcagtggc gtccatatc 
      actcttcct gggtattac agcccggag cagatgggt 
      ttgcttgac cccagtacc agtgacggt cgcgtgatt 
      ttcttcctg ccgtggcag aagcagacc atcgctggt 
      acgacggac ctcccatgc gacgtcacc caccatccc 
      aagcccacc gaggacgag attttattt attttagaa 
      gaagttaag aattatttg aatccggat gtggaagtg 
      aggcgaggt gatgtcttg tcggcctgg agcggtata 
      aggccactc gtttccgat ccgaataag ccggacacg 
      cagtcgctt gcccgtaac cacattgtt catgttagt 
      gatagtaaa ttggtaacg atagctggg ggtaaatgg 
      accacgtat cgtgccatg gctcaggag actattgat 
      gcggcgatt gccgcctgt tgtctcaag ccggtgcat 
      aaagagtgt cagactgac gggttgttg ctggagggg 
      gcgcacgga tggaccccc accatgtac attcggtta 
      gtgcaggat ttcggcctg gagtgcgag gtggcgcaa 
      catttgtcc aagtcctac ggcgacagg gcgttcacg 
      gtggccaag atggcagcg ctgacaggg aagcggtgg 
      cccatcatc ggcaaaaaa atccacccc gagttcccg 
      tacatcgac gccgaggtc agatacggc gtccgagag 
      tacgcagtg actgccgtc gacatgatc gcacgaagg 
      cttcgtttg gctttcctc aacgtgcaa gccgctcaa 
      gaagccctc cctgagatt atcacaatc atggccgag 
      gagttaggc tggtctgac gaggagaag caaaagcag 
      cacaaagac gccacaatc ttcctccag aacgagatg 
      ggccagaac gtgaaccgc gccagcaga gacaaaatc 
      ccgatcaat ttgagcaaa gaagagata cagttgtac 
      attaagcgg ttccaaatt atcgacaag gaccgcaag 
      ggatacgtc tcgatcaat gatataagg aggagtctc 
      aagtctcta ggtatgaaa ctaaccgac gacgaaatc 
      caccacctc ttatccgat attaacgta acttacaac 
      ggccaattg gaactggct gattacttg cagatgatg 
      tctgccatc aagtcaggc catgtgacg tattcgaga 
      ttcgcccga atggctgaa ctcgaagaa caaaaacac 
      gagaaagaa atgctcaag aagaagatt tcggtcgag 
      cgcagcggt ggcggcgtg taa
    • >TC014609 RB
      atggcctca acccttgga aaaatcgca ctgggtggc 
      ctcaccacc acgctgggt gccggccta gcctcttac 
      ctcttcctc acagacgac aaagacaat cgatggggc 
      gccgtttac gcccaacac agcccccct aaggccaaa 
      cgcaacctc ccgagccgc gaggaacaa ataaaatcg 
      ttgcagaac gactccttc gatgtgctt atcgtcgga 
      ggtggggcc acaggggct ggatgtgcg ctagacgcc 
      gtcaccagg gggctcaag acggctctc gtggaggcc 
      gacgatttc gcaagtggg acttctagt cgcagcacg 
      aagctgatc catggaggg gtgcgctac ctccagaag 
      gcgatcatg cagctggat atcgagcag tataggatg 
      gtcaaagag gcgctgcat gagagggcg tctatgtta 
      cagtcagcc ccacacttg gcgcatcct ctgcccatc 
      atgctcccc gtttatacg tggtggcaa gtgccatat 
      tactgggtt ggcataaaa atgtacgac gttgtcgct 
      ggtcggaag accctcaaa tcatcgtat ctcctctca 
      aagaaaaac gccttggag ttgtttcca atgcttcga 
      ggagacaaa ctttgtggt gctatagtt tattacgac 
      ggccaacaa gacgacgcc cgaatgaat ctcgccatt 
      gctttaaca gccacccgt cacggcgcc actgtggca 
      aaccacgtt tctgtgacc ggcctcctc aaagaaaaa 
      gattcttcc ggcaaagaa atcgtgtgt ggcgtcagt 
      gtcaaggac gaaatcacc ggaaacaaa tggaatgtc 
      ccggcaaaa tgtgtgata aacgccact ggacccttc 
      acggactcc atccggaag atggacaat ccggaagtg 
      aaaacgatt tgttcgccc agcagtggc gtccatatc 
      actcttcct gggtattac agcccggag cagatgggt 
      ttgcttgac cccagtacc agtgacggt cgcgtgatt 
      ttcttcctg ccgtggcag aagcagacc atcgctggt 
      acgacggac ctcccatgc gacgtcacc caccatccc 
      aagcccacc gaggacgag attttattt attttagaa 
      gaagttaag aattatttg aatccggat gtggaagtg 
      aggcgaggt gatgtcttg tcggcctgg agcggtata 
      aggccactc gtttccgat ccgaataag ccggacacg 
      cagtcgctt gcccgtaac cacattgtt catgttagt 
      gatagtaaa ttggtaacg atagctggg ggtaaatgg 
      accacgtat cgtgccatg gctcaggag actattgat 
      gcggcgatt gccgcctgt tgtctcaag ccggtgcat 
      aaagagtgt cagactgac gggttgttg ctggagggg 
      gcgcacgga tggaccccc accatgtac attcggtta 
      gtgcaggat ttcggcctg gagtgcgag gtggcgcaa 
      catttgtcc aagtcctac ggcgacagg gcgttcacg 
      gtggccaag atggcagcg ctgacaggg aagcggtgg 
      cccatcatc ggcaaaaaa atccacccc gagttcccg 
      tacatcgac gccgaggtc agatacggc gtccgagag 
      tacgcagtg actgccgtc gacatgatc gcacgaagg 
      cttcgtttg gctttcctc aacgtgcaa gccgctcaa 
      gaagccctc cctgagatt atcacaatc atggccgag 
      gagttaggc tggtctgac gaggagaag caaaagcag 
      cacaaagac gccacaatc ttcctccag aacgagatg 
      ggccagaac gtgaaccgc gccagcaga gacaaaatc 
      ccgatcaat ttgagcaaa gaagagata cagttgtac 
      attaagcgg ttccaaatt atcgacaag gaccgcaag 
      ggatacgtc tcgatcaat gatataagg aggagtctc 
      aagtctcta ggtatgaaa ctaaccgac gacgaaatc 
      caccacctc ttatccgat attaacgta acttacaac 
      ggccaattg gaactggct gattacttg cagcgggat 
      gatggaaag gaggttcct ggtgaggag ctccacgaa 
      atccttcgg gagattgat acgaatatg aatggacaa 
      gttgaactt gacgagtat ttgcagatg atgtctgcc 
      atcaagtca ggccatgtg acgtattcg agattcgcc 
      cgaatggct gaactcgaa gaacaaaaa cacgagaaa 
      gaaatgctc aagaagaag atttcggtc gagcgcagc 
      ggtggcggc gtgtaa
    mRNA sequence
    • >TC014609 RA
      tcacagttt gtaaattgg aaaaatggc ctcaaccct 
      tggaaaaat cgcactggg tggcctcac caccacgct 
      gggtgccgg cctagcctc ttacctctt cctcacaga 
      cgacaaaga caatcgatg gggcgccgt ttacgccca 
      acacagccc ccctaaggc caaacgcaa cctcccgag 
      ccgcgagga acaaataaa atcgttgca gaacgactc 
      cttcgatgt gcttatcgt cggaggtgg ggccacagg 
      ggctggatg tgcgctaga cgccgtcac cagggggct 
      caagacggc tctcgtgga ggccgacga tttcgcaag 
      tgggacttc tagtcgcag cacgaagct gatccatgg 
      aggggtgcg ctacctcca gaaggcgat catgcagct 
      ggatatcga gcagtatag gatggtcaa agaggcgct 
      gcatgagag ggcgtctat gttacagtc agccccaca 
      cttggcgca tcctctgcc catcatgct ccccgttta 
      tacgtggtg gcaagtgcc atattactg ggttggcat 
      aaaaatgta cgacgttgt cgctggtcg gaagaccct 
      caaatcatc gtatctcct ctcaaagaa aaacgcctt 
      ggagttgtt tccaatgct tcgaggaga caaactttg 
      tggtgctat agtttatta cgacggcca acaagacga 
      cgcccgaat gaatctcgc cattgcttt aacagccac 
      ccgtcacgg cgccactgt ggcaaacca cgtttctgt 
      gaccggcct cctcaaaga aaaagattc ttccggcaa 
      agaaatcgt gtgtggcgt cagtgtcaa ggacgaaat 
      caccggaaa caaatggaa tgtcccggc aaaatgtgt 
      gataaacgc cactggacc cttcacgga ctccatccg 
      gaagatgga caatccgga agtgaaaac gatttgttc 
      gcccagcag tggcgtcca tatcactct tcctgggta 
      ttacagccc ggagcagat gggtttgct tgaccccag 
      taccagtga cggtcgcgt gattttctt cctgccgtg 
      gcagaagca gaccatcgc tggtacgac ggacctccc 
      atgcgacgt cacccacca tcccaagcc caccgagga 
      cgagatttt atttatttt agaagaagt taagaatta 
      tttgaatcc ggatgtgga agtgaggcg aggtgatgt 
      cttgtcggc ctggagcgg tataaggcc actcgtttc 
      cgatccgaa taagccgga cacgcagtc gcttgcccg 
      taaccacat tgttcatgt tagtgatag taaattggt 
      aacgatagc tgggggtaa atggaccac gtatcgtgc 
      catggctca ggagactat tgatgcggc gattgccgc 
      ctgttgtct caagccggt gcataaaga gtgtcagac 
      tgacgggtt gttgctgga gggggcgca cggatggac 
      ccccaccat gtacattcg gttagtgca ggatttcgg 
      cctggagtg cgaggtggc gcaacattt gtccaagtc 
      ctacggcga cagggcgtt cacggtggc caagatggc 
      agcgctgac agggaagcg gtggcccat catcggcaa 
      aaaaatcca ccccgagtt cccgtacat cgacgccga 
      ggtcagata cggcgtccg agagtacgc agtgactgc 
      cgtcgacat gatcgcacg aaggcttcg tttggcttt 
      cctcaacgt gcaagccgc tcaagaagc cctccctga 
      gattatcac aatcatggc cgaggagtt aggctggtc 
      tgacgagga gaagcaaaa gcagcacaa agacgccac 
      aatcttcct ccagaacga gatgggcca gaacgtgaa 
      ccgcgccag cagagacaa aatcccgat caatttgag 
      caaagaaga gatacagtt gtacattaa gcggttcca 
      aattatcga caaggaccg caagggata cgtctcgat 
      caatgatat aaggaggag tctcaagtc tctaggtat 
      gaaactaac cgacgacga aatccacca cctcttatc 
      cgatattaa cgtaactta caacggcca attggaact 
      ggctgatta cttgcagat gatgtctgc catcaagtc 
      aggccatgt gacgtattc gagattcgc ccgaatggc 
      tgaactcga agaacaaaa acacgagaa agaaatgct 
      caagaagaa gatttcggt cgagcgcag cggtggcgg 
      cgtgtaaat taaatttgt gaataaaaa ttgtgtcaa 
      tttgctact caggtgatt ttgtcgtat cgtttgagt 
      taatttatt ttgttgtag ctctcgttt taaataaaa 
      taaaaagta ccacaaaca
    • >TC014609 RB
      tcacagttt gtaaattgg aaaaatggc ctcaaccct 
      tggaaaaat cgcactggg tggcctcac caccacgct 
      gggtgccgg cctagcctc ttacctctt cctcacaga 
      cgacaaaga caatcgatg gggcgccgt ttacgccca 
      acacagccc ccctaaggc caaacgcaa cctcccgag 
      ccgcgagga acaaataaa atcgttgca gaacgactc 
      cttcgatgt gcttatcgt cggaggtgg ggccacagg 
      ggctggatg tgcgctaga cgccgtcac cagggggct 
      caagacggc tctcgtgga ggccgacga tttcgcaag 
      tgggacttc tagtcgcag cacgaagct gatccatgg 
      aggggtgcg ctacctcca gaaggcgat catgcagct 
      ggatatcga gcagtatag gatggtcaa agaggcgct 
      gcatgagag ggcgtctat gttacagtc agccccaca 
      cttggcgca tcctctgcc catcatgct ccccgttta 
      tacgtggtg gcaagtgcc atattactg ggttggcat 
      aaaaatgta cgacgttgt cgctggtcg gaagaccct 
      caaatcatc gtatctcct ctcaaagaa aaacgcctt 
      ggagttgtt tccaatgct tcgaggaga caaactttg 
      tggtgctat agtttatta cgacggcca acaagacga 
      cgcccgaat gaatctcgc cattgcttt aacagccac 
      ccgtcacgg cgccactgt ggcaaacca cgtttctgt 
      gaccggcct cctcaaaga aaaagattc ttccggcaa 
      agaaatcgt gtgtggcgt cagtgtcaa ggacgaaat 
      caccggaaa caaatggaa tgtcccggc aaaatgtgt 
      gataaacgc cactggacc cttcacgga ctccatccg 
      gaagatgga caatccgga agtgaaaac gatttgttc 
      gcccagcag tggcgtcca tatcactct tcctgggta 
      ttacagccc ggagcagat gggtttgct tgaccccag 
      taccagtga cggtcgcgt gattttctt cctgccgtg 
      gcagaagca gaccatcgc tggtacgac ggacctccc 
      atgcgacgt cacccacca tcccaagcc caccgagga 
      cgagatttt atttatttt agaagaagt taagaatta 
      tttgaatcc ggatgtgga agtgaggcg aggtgatgt 
      cttgtcggc ctggagcgg tataaggcc actcgtttc 
      cgatccgaa taagccgga cacgcagtc gcttgcccg 
      taaccacat tgttcatgt tagtgatag taaattggt 
      aacgatagc tgggggtaa atggaccac gtatcgtgc 
      catggctca ggagactat tgatgcggc gattgccgc 
      ctgttgtct caagccggt gcataaaga gtgtcagac 
      tgacgggtt gttgctgga gggggcgca cggatggac 
      ccccaccat gtacattcg gttagtgca ggatttcgg 
      cctggagtg cgaggtggc gcaacattt gtccaagtc 
      ctacggcga cagggcgtt cacggtggc caagatggc 
      agcgctgac agggaagcg gtggcccat catcggcaa 
      aaaaatcca ccccgagtt cccgtacat cgacgccga 
      ggtcagata cggcgtccg agagtacgc agtgactgc 
      cgtcgacat gatcgcacg aaggcttcg tttggcttt 
      cctcaacgt gcaagccgc tcaagaagc cctccctga 
      gattatcac aatcatggc cgaggagtt aggctggtc 
      tgacgagga gaagcaaaa gcagcacaa agacgccac 
      aatcttcct ccagaacga gatgggcca gaacgtgaa 
      ccgcgccag cagagacaa aatcccgat caatttgag 
      caaagaaga gatacagtt gtacattaa gcggttcca 
      aattatcga caaggaccg caagggata cgtctcgat 
      caatgatat aaggaggag tctcaagtc tctaggtat 
      gaaactaac cgacgacga aatccacca cctcttatc 
      cgatattaa cgtaactta caacggcca attggaact 
      ggctgatta cttgcagcg ggatgatgg aaaggaggt 
      tcctggtga ggagctcca cgaaatcct tcgggagat 
      tgatacgaa tatgaatgg acaagttga acttgacga 
      gtatttgca gatgatgtc tgccatcaa gtcaggcca 
      tgtgacgta ttcgagatt cgcccgaat ggctgaact 
      cgaagaaca aaaacacga gaaagaaat gctcaagaa 
      gaagatttc ggtcgagcg cagcggtgg cggcgtgta 
      aattaaatt tgtgaataa aaattgtgt caatttgct 
      actcaggtg attttgtcg tatcgtttg agttaattt 
      attttgttg tagctctcg ttttaaata aaataaaaa 
      gtaccacaa aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY