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Gene information
General information
TC013217 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12097100..12098889
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013217
    iBeetle screen iB_08374

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013217 RA
      MARKSHDPV SSQLSNTQI VLTSLCAGA IAGALAKTT 
      IAPLDRTKI NFQISNKPY STRKAFKFL RQTYHQHGF 
      LALWRGNSA TMVRIVPHA AIQFTAHEQ WKKILNVDN 
      TNKSPRKLF LAGSLAGAT SQSLTYPLD VARARMAVT 
      NKQEYATLR QVFYKIFYE EGITAFYKG YIPTIAGVV 
      PYAGVSFFT YDTLKMLYR EYTNLDCDA RLNPVISLG 
      FGAIAGMLG QCSSYPLDI VRRRMQTDT QGKYNSIRA 
      TLKIIYKEG IIGGFYKGL SMNWIKGPI AVGISYSSY 
      DNIKNTLRQ LTAKYNRKF L
    CDS sequence
    • >TC013217 RA
      atggctcgt aaaagccat gaccctgtt tcgagtcag 
      ctgagcaac actcagatc gtcctcact agtctgtgt 
      gcgggcgcc atagcgggg gccctcgcc aagaccacg 
      atagccccc ttggaccgc acaaaaatc aacttccag 
      atcagcaac aaaccctat tcaactcgc aaggcgttc 
      aagtttctg cgacaaacg taccaccag cacgggttt 
      ctggcccta tggaggggc aacagtgcc accatggtg 
      cgcattgtg ccccatgcc gccatccag tttaccgca 
      cacgaacag tggaagaaa attctcaat gtggacaat 
      acaaacaag tcccccagg aaattgttc ctggcgggg 
      agtctcgcg ggggccact tcgcaatct ctcacctac 
      ccacttgat gtggcgagg gctcgaatg gctgtcacg 
      aataagcag gaatacgcg actttgaga caagttttt 
      tataagatt ttttacgaa gagggaatc acagcgttc 
      tataagggg tacataccc accattgcg ggggtcgtg 
      ccctatgca ggggtctcg ttttttact tatgatacc 
      ttgaaaatg ctctataga gaatatacg aatttagat 
      tgtgatgct cgtttaaat cctgtcatt agtctcggt 
      tttggggct atcgcagga atgttgggt cagtgtagt 
      agctatccg ttagatatt gtaagaaga cgaatgcag 
      actgatact caaggaaaa tataattca attagagca 
      actcttaaa ataatttat aaggaagga ataatcggg 
      ggtttttac aaaggtttg tccatgaac tggatcaaa 
      ggccccatc gcggttgga ataagctat tccagctac 
      gacaacata aaaaacacg ttacgtcaa ttaacagcg 
      aaatataat agaaagttt ttgtag
    mRNA sequence
    • >TC013217 RA
      atcactcca cgcttagat gttgttaaa tccccgtcc 
      aattaaaaa aaggtaaca cactttgaa ctttgcctc 
      ctttcctcc gactctcct ctaattctt cattcccgt 
      tccagttga cccaataat gccttgatt agtcttcca 
      aaaaatggc tcgtaaaag ccatgaccc tgtttcgag 
      tcagctgag caacactca gatcgtcct cactagtct 
      gtgtgcggg cgccatagc gggggccct cgccaagac 
      cacgatagc ccccttgga ccgcacaaa aatcaactt 
      ccagatcag caacaaacc ctattcaac tcgcaaggc 
      gttcaagtt tctgcgaca aacgtacca ccagcacgg 
      gtttctggc cctatggag gggcaacag tgccaccat 
      ggtgcgcat tgtgcccca tgccgccat ccagtttac 
      cgcacacga acagtggaa gaaaattct caatgtgga 
      caatacaaa caagtcccc caggaaatt gttcctggc 
      ggggagtct cgcgggggc cacttcgca atctctcac 
      ctacccact tgatgtggc gagggctcg aatggctgt 
      cacgaataa gcaggaata cgcgacttt gagacaagt 
      tttttataa gatttttta cgaagaggg aatcacagc 
      gttctataa ggggtacat acccaccat tgcgggggt 
      cgtgcccta tgcaggggt ctcgttttt tacttatga 
      taccttgaa aatgctcta tagagaata tacgaattt 
      agattgtga tgctcgttt aaatcctgt cattagtct 
      cggttttgg ggctatcgc aggaatgtt gggtcagtg 
      tagtagcta tccgttaga tattgtaag aagacgaat 
      gcagactga tactcaagg aaaatataa ttcaattag 
      agcaactct taaaataat ttataagga aggaataat 
      cgggggttt ttacaaagg tttgtccat gaactggat 
      caaaggccc catcgcggt tggaataag ctattccag 
      ctacgacaa cataaaaaa cacgttacg tcaattaac 
      agcgaaata taatagaaa gtttttgta gtaattttt 
      atatattat tgtattgtt tgttatcgg aatgttgat 
      tatttaaca ggatttata cattttgtt acactggaa 
      taaaaaaat ggaaaagtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY