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Gene information
General information
TC013230 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11860840..11902906
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
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    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08369

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC013230 RA
    CDS sequence
    • >TC013230 RA
      atgaagtgt gtgtgtgtg gaagcgtcc ggctacgaa 
      cgcctcaat gagaatttc ctcgattat caaatcgta 
      tcagaagat aaggataat gcgacaaat gaaaaagag 
      gaggaaatg gtcttttac aacgacgac ggcagtttc 
      cagaccgtc atcgtcgag cgaaatctg tgtgcgagc 
      gacttgtgc caacttctt gccctcaaa aatcgcgtg 
      tcaaaaagt gtcaattgg agcattgtc gaacattgg 
      gtggaatac ggtttagag cgttcgttc gaagatcac 
      gaatacgtt ctcgaagcg tcgagagat atgaggggg 
      tattcgagg cacgcccaa tttagatac gttttcaga 
      aaagactac aggaaatac gagttcttc cacaatcca 
      caacagttc ttcccctcc gatatgtta aacgtcacc 
      aaagaagac ttccctcat ctcacaaac ttcaacgaa 
      aactacctc cagaatctc gtgttacaa gaaggaaaa 
      tcgccggtg gttttttcg caagtgtgg attaaagat 
      caacagaaa caggcctgg ggtaaggcc tttctgtta 
      ctccgtgaa aagaagctc tatctctcg tacaagatc 
      caagtgttg gcgaaattc ctgagggct gcgaaaatc 
      agggaaact tcggtttat ttgttggtt ttaatggag 
      ccattagag ctcggcctt cgcctggta cgggccatc 
      tccttcatc aggccgttt ttcctcgca ttttcgcca 
      cacttgacg tcagattcg aacgatttg gaagttgtg 
      tgtaaaggc gaagtcaat gccgttagc cgccgactc 
      cggagaccg gcagccgcc tgcctgaaa acagttgaa 
      cgccggcaa acgagtggc aatgtggtc gagatagca 
      gtagtatcg aatctaagt acaccggca aacacttgc 
      aataatttt ttttcattt cgttatttt aacaccgaa 
      aatggaccg agtgataaa gtcgactgc cattatttc 
      cataatcga aaacaatca ttgatctca gagtcggac 
      atcaacggc ttgaatttc ttcgccgaa ctgtccaac 
      taccacgtt tacacgaca ctcaacgca aagaaccag 
      ttccgtgcc cccaccgag tggggcatc tgcttgagg 
      ccttcagga gtcgacacc gaagacgaa actccagat 
      ttgaagtgt atagcttgt gattcggag cgagtacgg 
      tcctgttgg ctgaccgcc atgaggctt gccaagtat 
      ggaaagcaa ctacgcgaa aactacaga gccttcaaa 
      aacaagcaa accgagtcc atcaatcca aaagactac 
      aacagctac acagtacct aatgaatct gtcagatcg 
      agggtcgca atggacttt accggctcg gttgggcgc 
      atagtcgag gacccgaag gaagcgaaa gcgatcgcg 
      gtggctgag ggctattcg tggaagcgg cggtggcgg 
      cccccttgt cgcccgccc ccgaacagc gcatatcca 
      cagggcctc gaatcgggg gtgcacata acgcagccc 
      tggtttcac agcggcatg agcagggac caagcaacg 
      gcactagtc acgaaacac ggaaccgtc gacggggtg 
      tttttggtg cgggagagc cgatcgaat ccaggggcg 
      ttcgtcttg acgtacaaa tgcggcggt aaagtgttg 
      cacgcccaa atcacgccg atcctcgac cccgtccga 
      gaggctcac gttttctct ctcgacagt ggcgtaacg 
      aagttctac gatctcttg caactcgtc gaattttat 
      caattgaat gctggatgt ttacccacg agactcact 
      cattacgta gtacagaat tgttcgccg gtgaattcg 
      tcgacgaat ccgacgacg acgacaacg acggtgtcg 
      gctagcatt taa
    mRNA sequence
    • >TC013230 RA
      aggaggcat tttactacg agcatgcgt ctttcagcg 
      tattcgtgt ttacttacg gtttatttt tctattcgt 
      ccagtctgc aatcggcat tccgagtgt ttaggtgca 
      attcctagt ttgaatttc gcgccataa agccagaca 
      ttcgtttgt tgtcgcgga ccatcacta gatcctaga 
      catcgttag ctaatttcg cgtttttag tgcgtatta 
      tggacacgt gttggtttt aatcgctgc cgcagctgt 
      gcaaagggc tagggtctc tccaggggc ggctagaat 
      ggatttcgg agccctcac gaggcacaa ccactggtg 
      ccgctttcc gaggatgaa gtgtgtgtg tgtggaagc 
      gtccggcta cgaacgcct caatgagaa tttcctcga 
      ttatcaaat cgtatcaga agataagga taatgcgac 
      aaatgaaaa agaggagga aatggtctt ttacaacga 
      cgacggcag tttccagac cgtcatcgt cgagcgaaa 
      tctgtgtgc gagcgactt gtgccaact tcttgccct 
      caaaaatcg cgtgtcaaa aagtgtcaa ttggagcat 
      tgtcgaaca ttgggtgga atacggttt agagcgttc 
      gttcgaaga tcacgaata cgttctcga agcgtcgag 
      agatatgag ggggtattc gaggcacgc ccaatttag 
      atacgtttt cagaaaaga ctacaggaa atacgagtt 
      cttccacaa tccacaaca gttcttccc ctccgatat 
      gttaaacgt caccaaaga agacttccc tcatctcac 
      aaacttcaa cgaaaacta cctccagaa tctcgtgtt 
      acaagaagg aaaatcgcc ggtggtttt ttcgcaagt 
      gtggattaa agatcaaca gaaacaggc ctggggtaa 
      ggcctttct gttactccg tgaaaagaa gctctatct 
      ctcgtacaa gatccaagt gttggcgaa attcctgag 
      ggctgcgaa aatcaggga aacttcggt ttatttgtt 
      ggttttaat ggagccatt agagctcgg ccttcgcct 
      ggtacgggc catctcctt catcaggcc gtttttcct 
      cgcattttc gccacactt gacgtcaga ttcgaacga 
      tttggaagt tgtgtgtaa aggcgaagt caatgccgt 
      tagccgccg actccggag accggcagc cgcctgcct 
      gaaaacagt tgaacgccg gcaaacgag tggcaatgt 
      ggtcgagat agcagtagt atcgaatct aagtacacc 
      ggcaaacac ttgcaataa ttttttttc atttcgtta 
      ttttaacac cgaaaatgg accgagtga taaagtcga 
      ctgccatta tttccataa tcgaaaaca atcattgat 
      ctcagagtc ggacatcaa cggcttgaa tttcttcgc 
      cgaactgtc caactacca cgtttacac gacactcaa 
      cgcaaagaa ccagttccg tgcccccac cgagtgggg 
      catctgctt gaggccttc aggagtcga caccgaaga 
      cgaaactcc agatttgaa gtgtatagc ttgtgattc 
      ggagcgagt acggtcctg ttggctgac cgccatgag 
      gcttgccaa gtatggaaa gcaactacg cgaaaacta 
      cagagcctt caaaaacaa gcaaaccga gtccatcaa 
      tccaaaaga ctacaacag ctacacagt acctaatga 
      atctgtcag atcgagggt cgcaatgga ctttaccgg 
      ctcggttgg gcgcatagt cgaggaccc gaaggaagc 
      gaaagcgat cgcggtggc tgagggcta ttcgtggaa 
      gcggcggtg gcggccccc ttgtcgccc gcccccgaa 
      cagcgcata tccacaggg cctcgaatc gggggtgca 
      cataacgca gccctggtt tcacagcgg catgagcag 
      ggaccaagc aacggcact agtcacgaa acacggaac 
      cgtcgacgg ggtgttttt ggtgcggga gagccgatc 
      gaatccagg ggcgttcgt cttgacgta caaatgcgg 
      cggtaaagt gttgcacgc ccaaatcac gccgatcct 
      cgaccccgt ccgagaggc tcacgtttt ctctctcga 
      cagtggcgt aacgaagtt ctacgatct cttgcaact 
      cgtcgaatt ttatcaatt gaatgctgg atgtttacc 
      cacgagact cactcatta cgtagtaca gaattgttc 
      gccggtgaa ttcgtcgac gaatccgac gacgacgac 
      aacgacggt gtcggctag catttaata atgcatttg 
      actgacttt tcttcaaag tgactttaa tttattttt 
      gattgttga ttttcgagt gacgtggcc gcgaaccaa 
      agtgaaaac ggatcgaat tttaactgg tccgccttg 
      taaaagaca ctcaagcac tgattttac cctagttgt 
      aatgccgag atttttccc ggacttgtg ttgctccag 
      cgattttta cagggatga tatttattt ttgttcttt 
      cgtatttat gtttaatat gtatttcag tattaaatc 
      tcattgata acagggtga aaaaaacta gtttttaaa 
      atctctttt tcgaaaaat agatgagaa aggctgtgg 
      ttgttctgg tgtggatta gaaaaatat atttgatat 
      ttataaaga aattgttaa ttgaaattc acaccgttt 
      gtatagctt taggattag gtaaaacga ttttttttc 
      gttctttaa ttaaatatc ataggtata ataattaaa 
      aaccaaaaa atatattat ttaaattag gaaagcaat 
      atactagta aaggacttc tcgtagatg tgatattta 
      gttaagtat tttttcatt tacacagat taatcgtta 
      ttatttaaa cgcagtatt tttgtaaaa ccgtgcttt 
      tgcccctac ccatacatc attaacatt atttatgta 
      tttttcctt ttttaacgt ttttttctc aatcatcct 
      tgtaagtta tataaagcg aaaaaatca catatgtgg 
      acatataga aatagtact tacgcaaac agtgacaat 
      tactgcagc ttgtgttcg ttgtgactg agctgattc 
      tttgatacc gttcttgtt tattccact tactgtaca 
      tccattatt ttgtactct tgtagatga caacatgaa 
      gtaaattta acacttaat ataagcagt cttttcat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY