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Gene information
General information
TC013630 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2901788..2909655
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013630
    iBeetle screen iB_08367

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    vitellogenic egg chamber fused - Number of animals showing the phenotype: 3

    egg chamber pattern irregular - Number of animals showing the phenotype: 2

    mature egg number increased - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva segment random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013630 RA
      MPRPPRETY GDQKPPYSY ISLTAMAIW SSPEKMLPL 
SEIYRFITD RFPYYRRNT QRWQNSLRH NLSFNDCFI 
KIPRRPDRP GKGAFWALH PSALDMFEN GSFLRRRKR 
FKLPKHVHE ELDSLSKLN SSFMGGQTI SPTPSACPP 
IVCPPELPL PPITPVATT APKSFSIDS IIQSEPKSP 
QFPTNVPPI QALPSLSWV NMMTPSTSQ SELQSRDDL 
IMAQKAAFF EYAAAIFSI NASTSAKTG TGVIRPLPI 
MPNALYPAT CHTVPVPVQ YSFCMPNMR NLEFETEHE 
CDRTIMDKN ISLNYLIAQ NGQEDNIEI SSSSSEEAT 
NV
    CDS sequence
    • >TC013630 RA
      atgccaagg ccacctcgt gaaacatac ggggaccag 
aaaccacca tactcgtac atatctctc acagcgatg 
gccatctgg agctccccg gagaaaatg ctacccctt 
tcggagatc taccgattc atcaccgac cgcttcccc 
tactaccgg cggaacacc cagaggtgg caaaactcc 
ctgcgacac aacctctcc ttcaacgac tgcttcatc 
aagatcccg cggcggccc gacaggccc ggcaagggc 
gccttctgg gccctgcac ccctcggcc ctggacatg 
ttcgagaac gggagcttc ctcagacgg cggaagcgc 
ttcaagctg cccaagcac gtccacgag gagctcgac 
agcctctcg aaactgaac agttccttc atggggggg 
cagaccatc agcccgacc ccgtccgcc tgccccccg 
atcgtgtgc ccccccgag ctgccgctc ccgcccatc 
acccccgtg gccaccacc gcccccaag tccttcagc 
atcgacagc atcatccaa agcgagccg aagtcgccg 
cagttcccc acgaacgtg cccccgatc caggccctc 
cctagcctg tcctgggtc aacatgatg accccctcc 
acctcccag agcgagctc cagagccga gacgacttg 
atcatggcc cagaaagcg gcgtttttc gaatacgcc 
gccgccatt ttttcgata aatgcctcg acgagcgcg 
aagacgggc actggggtc atacgaccc ctgcccatc 
atgcccaac gctctctac ccggcgacc tgccatacg 
gtaccggta ccagtgcaa tactccttc tgcatgccc 
aatatgcgc aacttggaa tttgagact gagcacgaa 
tgtgataga accatcatg gacaaaaat atctcactc 
aactattta atcgcccag aatggccaa gaggacaac 
atcgaaatc tcctcctcg tcctcggag gaggccacc 
aacgtgtaa
    mRNA sequence
    • >TC013630 RA
      acacatttt tgatcacac tctcttaga taaaccgat 
ttgtaaagt taactacaa agctaagcc gacaatccc 
atcagaaaa taagccgac agtgatttt ctttgtgcg 
gttgctgaa caccattat gtacactgg tgcggtata 
gtgtttgtg tacgaagga ctgtacaga tttatttat 
ttaatttgt catgtggca caaatggat aaaggggac 
aacttaaaa gtcgtcagc agaaatgcc aaggccacc 
tcgtgaaac atacgggga ccagaaacc accatactc 
gtacatatc tctcacagc gatggccat ctggagctc 
cccggagaa aatgctacc cctttcgga gatctaccg 
attcatcac cgaccgctt cccctacta ccggcggaa 
cacccagag gtggcaaaa ctccctgcg acacaacct 
ctccttcaa cgactgctt catcaagat cccgcggcg 
gcccgacag gcccggcaa gggcgcctt ctgggccct 
gcacccctc ggccctgga catgttcga gaacgggag 
cttcctcag acggcggaa gcgcttcaa gctgcccaa 
gcacgtcca cgaggagct cgacagcct ctcgaaact 
gaacagttc cttcatggg ggggcagac catcagccc 
gaccccgtc cgcctgccc cccgatcgt gtgcccccc 
cgagctgcc gctcccgcc catcacccc cgtggccac 
caccgcccc caagtcctt cagcatcga cagcatcat 
ccaaagcga gccgaagtc gccgcagtt ccccacgaa 
cgtgccccc gatccaggc cctccctag cctgtcctg 
ggtcaacat gatgacccc ctccacctc ccagagcga 
gctccagag ccgagacga cttgatcat ggcccagaa 
agcggcgtt tttcgaata cgccgccgc cattttttc 
gataaatgc ctcgacgag cgcgaagac gggcactgg 
ggtcatacg acccctgcc catcatgcc caacgctct 
ctacccggc gacctgcca tacggtacc ggtaccagt 
gcaatactc cttctgcat gcccaatat gcgcaactt 
ggaatttga gactgagca cgaatgtga tagaaccat 
catggacaa aaatatctc actcaacta tttaatcgc 
ccagaatgg ccaagagga caacatcga aatctcctc 
ctcgtcctc ggaggaggc caccaacgt gtaaatact 
ccgtgtgca aactgtgtt aatgtattt tagttctta 
tagttatat ttaaataaa agttgagaa gaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY