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Gene information
General information
TC013235 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11773750..11775289
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013235
    iBeetle screen iB_08366

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013235 RA
      MGKAKRLKP SQMGTKVPL DQDIEDAKF AKSKNRNKI 
      RLRQDDDEQ FVDSNLSRK ILSAAREQQ RELDSSFGP 
      TPSEASSSK FKVSKLGDD DSEPEEEDT LEPDTFYEN 
      IEINEEDEK ALEKFMSSN PAPRRTLGD ILAEKFTEV 
      KTHFSEEGS VKMQELDPR VEQMYQGVR DVLRKYRSG 
      KLPKAFKII PNLRNWEQI LYITDPPSW SAAAMYQAT 
      RIFASNLKE KMAQRFYNL VLLPRIRDD LAEYKRLNF 
      HLYQALRKA LFKPGGFMK GILLPLLES GDCTLREAI 
      IIGSVVARN SIPILHSSA AILKIAEMD YTGANSIFL 
      RIFFDKRYA LPYRVVDAV VFHFLRFER DSRELPVLW 
      HQAFLTFVQ RYKADISSE QRDSLLELL KKQNHHAIT 
      PEIRREIQN AKCRDAETA EPKMDFD
    CDS sequence
    • >TC013235 RA
      atgggcaaa gcaaagcgc ctgaagccg tctcagatg 
      ggcaccaag gtgcccctg gaccaggac atcgaggac 
      gcgaagttt gccaagtcg aaaaaccgc aacaaaatc 
      cgtctaaga caagacgac gatgaacaa tttgtggac 
      tcgaacctc tcgcggaag attctctca gccgcccga 
      gagcagcaa cgcgagctg gactcaagt ttcggcccc 
      acgccgtcc gaggcaagt tcaagcaag tttaaagtg 
      tccaaactg ggggacgat gactccgaa ccggaagag 
      gaagacacc ctcgaaccg gacacgttt tacgaaaac 
      attgaaatc aatgaagag gatgagaaa gctttagag 
      aagttcatg agtagcaat ccggccccg agacgcact 
      ttgggggat attttggcc gagaagttt accgaagtt 
      aagacgcat tttagcgag gaaggctcg gtcaaaatg 
      caggagttg gacccacgg gtggaacaa atgtatcaa 
      ggggtgagg gatgttttg cggaaatat aggagcggc 
      aagttgccg aaagcgttc aaaatcatc cccaacttg 
      cggaattgg gaacaaatc ttatacatc acggatccg 
      ccaagttgg tcagctgct gccatgtac caagccacc 
      cgtattttc gcctcaaac ctcaaggaa aaaatggct 
      cagaggttc tataatttg gttttgttg ccccgaatt 
      cgcgacgac ttggccgaa tacaagcga ctcaatttc 
      cacttatat caagccttg aggaaagcc ctgttcaaa 
      cctgggggc ttcatgaag ggaattctc ctaccactg 
      cttgaaagt ggcgattgt acgctcagg gaagctatt 
      ataataggc tcagttgtc gcaagaaac tctattcct 
      attttgcat tcttctgcc gcgattttg aaaatcgcg 
      gaaatggat tacaccggc gccaattcg atttttttg 
      cgtattttc ttcgataaa agatacgcg ttgccgtat 
      cgtgttgtg gacgcagtt gtgttccac tttttgaga 
      ttcgagcga gattccagg gaattaccc gttttgtgg 
      catcaggct ttcctgaca tttgtacaa agatacaaa 
      gctgatatt tcaagcgag caaagggac agtttatta 
      gaattatta aagaagcaa aaccaccac gctatcaca 
      ccagagatc agaagagaa attcaaaat gcgaaatgt 
      cgagacgct gaaaccgcc gaaccaaaa atggacttt 
      gactaa
    mRNA sequence
    • >TC013235 RA
      aatttaatt ttaaattgc caatgtcgc caacccaaa 
      cctctgaca agtcctttt aggttacgt ccagatagt 
      ttgaagaca cgtgtggta aacaaatag ggataatgg 
      gcaaagcaa agcgcctga agccgtctc agatgggca 
      ccaaggtgc ccctggacc aggacatcg aggacgcga 
      agtttgcca agtcgaaaa accgcaaca aaatccgtc 
      taagacaag acgacgatg aacaatttg tggactcga 
      acctctcgc ggaagattc tctcagccg cccgagagc 
      agcaacgcg agctggact caagtttcg gccccacgc 
      cgtccgagg caagttcaa gcaagttta aagtgtcca 
      aactggggg acgatgact ccgaaccgg aagaggaag 
      acaccctcg aaccggaca cgttttacg aaaacattg 
      aaatcaatg aagaggatg agaaagctt tagagaagt 
      tcatgagta gcaatccgg ccccgagac gcactttgg 
      gggatattt tggccgaga agtttaccg aagttaaga 
      cgcatttta gcgaggaag gctcggtca aaatgcagg 
      agttggacc cacgggtgg aacaaatgt atcaagggg 
      tgagggatg ttttgcgga aatatagga gcggcaagt 
      tgccgaaag cgttcaaaa tcatcccca acttgcgga 
      attgggaac aaatcttat acatcacgg atccgccaa 
      gttggtcag ctgctgcca tgtaccaag ccacccgta 
      ttttcgcct caaacctca aggaaaaaa tggctcaga 
      ggttctata atttggttt tgttgcccc gaattcgcg 
      acgacttgg ccgaataca agcgactca atttccact 
      tatatcaag ccttgagga aagccctgt tcaaacctg 
      ggggcttca tgaagggaa ttctcctac cactgcttg 
      aaagtggcg attgtacgc tcagggaag ctattataa 
      taggctcag ttgtcgcaa gaaactcta ttcctattt 
      tgcattctt ctgccgcga ttttgaaaa tcgcggaaa 
      tggattaca ccggcgcca attcgattt ttttgcgta 
      ttttcttcg ataaaagat acgcgttgc cgtatcgtg 
      ttgtggacg cagttgtgt tccactttt tgagattcg 
      agcgagatt ccagggaat tacccgttt tgtggcatc 
      aggctttcc tgacatttg tacaaagat acaaagctg 
      atatttcaa gcgagcaaa gggacagtt tattagaat 
      tattaaaga agcaaaacc accacgcta tcacaccag 
      agatcagaa gagaaattc aaaatgcga aatgtcgag 
      acgctgaaa ccgccgaac caaaaatgg actttgact 
      aaattaatg gttacataa ttgtgataa ttatattga 
      aaaaaatcg gttacaaca agactatta ataaaaact 
      tggaaaaat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY