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Gene information
General information
TC033188
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013250
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11551286..11553309
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033188
    iBeetle screen iB_08365

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033188 RA
      MATEAVRRA VQIIRNDNN SISLDVNAL QNILLRDEI 
      RNKKICVLS ITGTFRQGK SFLLNFFLR YLKLKHGSS 
      PLDPNSDWL DPIHGSHLS DFLWDHGSN AHTNGILMW 
      PEIFTIYKP SSNEEIAVV LLDTQGSYD NQTTVENSA 
      TIFALSTMI SSVQIYNVM RNLNQNDLE SVEMFGQYG 
      RLLTNDDCP FQDLWILVR DWLAPYERP FGIPGGESL 
      LQDRLYGQH DALPDEINE QRNNVRNLF QTINCSLLP 
      FPGERVAAD HTFNGDLNE INDNFKRYV KQLVERIFE 
      PENLVIKRF NGEEITAND LFQCFGTYT EILGDGLPH 
      VETRLAATI NNQHNDAVE SSFELYDRQ MHHNFSSIL 
      LLNNHHESC KTRALDNYH NTRLLGSSL VYDRYEQRL 
      IRKMDERFS IYRERFDFD RDRQLERER RRNQQVSLQ 
      QFRPSQSSS LVSRESLST ETIILQSLR IATGLAAIG 
      FGIYEIIRQ SSRDTGNSN TNNSSSTRR YSTDDSDST
    CDS sequence
    • >TC033188 RA
      atggcaact gaagctgtt cggagggct gttcaaatt 
      attcgtaat gacaataat tcaattagc ctcgacgta 
      aatgccctc caaaatatt cttttgagg gacgaaata 
      aggaataaa aaaatctgt gtcctttca ataacggga 
      actttcaga caaggcaaa tctttctta ttaaacttt 
      tttttgaga tatctgaaa ctaaagcat gggagttct 
      ccacttgat cccaattct gattggttg gatcccata 
      catgggtca catttgtcg gattttttg tgggatcat 
      gggtctaat gctcatacc aatggtatt ttaatgtgg 
      ccagagatt tttactatt tataaaccc tcgtcaaat 
      gaagagatt gcggttgtt ttgctcgat actcaaggc 
      tcatacgac aatcaaaca accgtagaa aattcggca 
      acgattttt gctcttagt acaatgata agttccgta 
      caaatttat aacgtgatg cgcaatttg aatcaaaac 
      gacttggag tctgtggag atgtttggc caatatggg 
      agattactc acaaatgat gattgcccg tttcaagat 
      ctttggatt ttagtccga gattggctt gcaccctac 
      gaaaggcca tttggtatt ccagggggt gagtctttg 
      cttcaagac aggctttac ggtcaacat gatgcgctt 
      cccgacgaa ataaatgag caaagaaat aatgtgcgc 
      aatttattt caaacaatt aattgctcc ctgttacca 
      tttcctgga gagcgggta gcggccgat catactttt 
      aacggagat ctaaatgaa attaatgac aattttaag 
      cgatacgtt aagcaacta gttgaacgc atttttgaa 
      ccagaaaac ctggtaata aaacgcttt aacggagaa 
      gagatcacc gccaatgat ttatttcaa tgttttggc 
      acgtatact gaaatactt ggggatggt ttaccacat 
      gttgaaaca agactagcg gcgacaata aataatcaa 
      cataacgac gctgttgaa agttctttt gaactatat 
      gatcgacaa atgcatcat aatttttcc tcaatttta 
      ttacttaat aatcaccac gaatcttgt aaaacaaga 
      gcacttgac aattatcat aatactaga ttattagga 
      agcagtctc gtgtatgat agatacgaa caaaggctt 
      atcaggaaa atggacgaa cggttttcg atttacagg 
      gaaagattc gattttgat agagatcgc caacttgaa 
      cgtgaacga cgaagaaat caacaagtg agtcttcag 
      cagttcaga ccatcacag tcttcgagc ctcgtttca 
      cgagaatcc ctttcaaca gaaacgata attcttcaa 
      tccttgcgt attgcaaca ggacttgcg gcaattggt 
      tttggcatt tatgaaata attcgtcaa tcatctaga 
      gatacagga aattctaat acaaataat tcttcttcc 
      acaaggcgg tactcaact gatgactct gattctaca 
      taa
    mRNA sequence
    • >TC033188 RA
      ttagtagtg gatgtgtat tcgaaaact tttaggttc 
      atttatatt gtttgtatt tgtatgtaa taaggtatt 
      gtttgacac aatggcaac tgaagctgt tcggagggc 
      tgttcaaat tattcgtaa tgacaataa ttcaattag 
      cctcgacgt aaatgccct ccaaaatat tcttttgag 
      ggacgaaat aaggaataa aaaaatctg tgtcctttc 
      aataacggg aactttcag acaaggcaa atctttctt 
      attaaactt ttttttgag atatctgaa actaaagca 
      tgggagttc tccacttga tcccaattc tgattggtt 
      ggatcccat acatgggtc acatttgtc ggatttttt 
      gtgggatca tgggtctaa tgctcatac caatggtat 
      tttaatgtg gccagagat ttttactat ttataaacc 
      ctcgtcaaa tgaagagat tgcggttgt tttgctcga 
      tactcaagg ctcatacga caatcaaac aaccgtaga 
      aaattcggc aacgatttt tgctcttag tacaatgat 
      aagttccgt acaaattta taacgtgat gcgcaattt 
      gaatcaaaa cgacttgga gtctgtgga gatgtttgg 
      ccaatatgg gagattact cacaaatga tgattgccc 
      gtttcaaga tctttggat tttagtccg agattggct 
      tgcacccta cgaaaggcc atttggtat tccaggggg 
      tgagtcttt gcttcaaga caggcttta cggtcaaca 
      tgatgcgct tcccgacga aataaatga gcaaagaaa 
      taatgtgcg caatttatt tcaaacaat taattgctc 
      cctgttacc atttcctgg agagcgggt agcggccga 
      tcatacttt taacggaga tctaaatga aattaatga 
      caattttaa gcgatacgt taagcaact agttgaacg 
      catttttga accagaaaa cctggtaat aaaacgctt 
      taacggaga agagatcac cgccaatga tttatttca 
      atgttttgg cacgtatac tgaaatact tggggatgg 
      tttaccaca tgttgaaac aagactagc ggcgacaat 
      aaataatca acataacga cgctgttga aagttcttt 
      tgaactata tgatcgaca aatgcatca taatttttc 
      ctcaatttt attacttaa taatcacca cgaatcttg 
      taaaacaag agcacttga caattatca taatactag 
      attattagg aagcagtct cgtgtatga tagatacga 
      acaaaggct tatcaggaa aatggacga acggttttc 
      gatttacag ggaaagatt cgattttga tagagatcg 
      ccaacttga acgtgaacg acgaagaaa tcaacaagt 
      gagtcttca gcagttcag accatcaca gtcttcgag 
      cctcgtttc acgagaatc cctttcaac agaaacgat 
      aattcttca atccttgcg tattgcaac aggacttgc 
      ggcaattgg ttttggcat ttatgaaat aattcgtca 
      atcatctag agatacagg aaattctaa tacaaataa 
      ttcttcttc cacaaggcg gtactcaac tgatgactc 
      tgattctac ataatctac taaaaccga tctttgtta 
      atattttgt atcttatta cttttttgg tagttaaat 
      aacttgcac cagtgtcgg ccactttaa ataaaaaaa 
      taagcagaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY